1.Thrombospondin 2 and tumors
Journal of International Oncology 2021;48(3):164-166
Thrombospondin (THBS) 2, as a member of the THBS family, is expressed in a variety of tumor cells and has important significance in the development and metastasis of tumors. In recent years, studies have reported that the specific role of THBS2 in different tumors is different, and it participates in a variety of biological processes, such as cell apoptosis, wound healing, angiogenesis and inflammation. At present, the role of THBS2 in tumorigenesis, development, and prognosis is not completely clear. Exploring the abnormal expression and mechanism of THBS2 in tumors is expected to provide a new method for tumor diagnosis and treatment.
2.Diagnosis and management of acute deep vein thrombosis of lower extremity
Zhengrong QIAO ; De SHI ; Ou JIAN ; Zhigang WANG
Chinese Journal of General Surgery 1993;0(01):-
ObjectiveTo explore the diagnosis and management of acute deep vein thrombosis(DVT) of lower extremity. Methods The clinical data of 96 patients with DVT were analysed retrospectively. ResultsThe diagnosis rate of DVT by vascular color ultrasounography was 97.8%, by MVC/SVO ratio combi ned with air plethysmagraphy was 97.2%, and by ECT was 100%. The MVO/SVC ratio, and diameter of calf and thigh in surgery group and in non surgery group showed significant difference in 7 days after operation (P
3.Effects of interferon-γ on expression of adhesion molecules in human umbilical cord mesenchymal stromal cells.
Rui-Yun QIAO ; Hai BAI ; Cun-Bang WANG ; Jian-Feng OU ; Hai-Ying ZHANG ; Qiang ZHAO
Journal of Experimental Hematology 2012;20(5):1195-1199
This study was purposed to investigate the effects of interferon (IFN)-γ on expression of adhesion molecules in mesenchymal stromal cells derived from human umbilical cord tissue (UC-MSC). The UC-MSC were isolated from human umbilical cord by tissue culture. The expressions of specific markers on UC-MSC were detected by flow cytometry in the physiological condition. The adipogenic and osteogenic induction of UC-MSC was detected by alizarin and Oil red O staining. UC-MSC were exposed to IFN-γ (100, 1 000, 10 000 U/ml) for 24 h, the expressions of CD54, CD58, CD44, CD49d, CD62p, CD62L, CD102 and CD106 on cell surface were detected using flow cytometry. The results showed that in physiological condition, UC-MSC extremely low expressed CD102, CD106, CD62P, CD62L, while the expression of CD54 was relatively high (41.58 ± 0.83)%. When stimulated by IFN-γ, the expression of CD102, CD106, CD62P, CD62L increased slightly, but still low (< 5%), while CD54 and CD58 upregulated concentration-dependently up to (59.66 ± 1.36)% and (43.96 ± 0.62)% respectively. The expression of CD49d upregulated to (51.33 ± 0.74)% when UC-MSC exposed to IFN-γ 100 U/ml. CD44 increased to (73.22 ± 1.93)% when UC-MSC exposed to IFN-γ 1 000 U/ml. It is concluded that IFN-γ can elevate significantly the expression of CD54, CD49d, CD44 and CD58, but has no significant effect on CD102, CD106, CD62P and CD62L expression on the surface of UC-MSC.
Cell Adhesion Molecules
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metabolism
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Cells, Cultured
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Humans
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Interferon-gamma
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pharmacology
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Mesenchymal Stromal Cells
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cytology
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drug effects
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metabolism
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Umbilical Cord
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cytology
4.Expressions of E-cadherin in non-small cell lung cancer and it correlation with prognosis.
Gui-bin QIAO ; Yi-long WU ; Wei OU ; Xue-ning YANG ; Wen-zhao ZHONG ; Jia-ying LIN ; Jian ZHAO ; Dan XIE ; Xin-yuan GUAN
Chinese Journal of Surgery 2005;43(14):913-917
OBJECTIVEThis study was to clarify E-cadherin expressions in non-small cell lung cancer (NSCLC) and its correlation with patients' prognosis.
METHODSTissue microarrays (TMAs) containing specimens from 365 different NSCLC were constructed, covering all stages and almost all histological types of this disease. Slides were immunohistochemically stained with antibodies against E-cadherin. Expression pattern of the protein was analyzed with relation to the clinicopathological. Correlations of the results with patients' overall survival were also examined.
RESULTSImmunohistochemical staining revealed that E-cadherin protein was localized mainly on membranes and the cytoplasm of NSCLC tumors cells. Reduced E-cadherin expression was evident in 32.1%. Reduced E-cadherin expression significantly correlated with lymph nodes metastasis (chi(2) = 16.430, P = 0.001), histological dedifferentiation (chi(2) = 9.243, P = 0.010) and advanced clinical stage (chi(2) = 9.421, P = 0.024). There was no significant difference in E-cadherin expression between squamous cell carcinoma and adenocarcinoma. E-cadherin reduced expression correlated with a poor prognosis (P < 0.0001) in univariate analysis. Multivariate analysis showed a significantly lower survival probability for patients with reduced E-cadherin (P < 0.001), and E-cadherin was an independent prognostic factor for survival of NSCLC patients.
CONCLUSIONSIt suggests that dysfunction of E-cadherin has an important impact in the progression of lung cancer. As an independent prognostic factor, expression of E-cadherin can predict outcome of different group, together with conventional prognostic factors, and subsequently make appropriate management.
Adult ; Aged ; Aged, 80 and over ; Cadherins ; biosynthesis ; Carcinoma, Non-Small-Cell Lung ; metabolism ; mortality ; secondary ; Female ; Follow-Up Studies ; Humans ; Immunohistochemistry ; Lung Neoplasms ; metabolism ; mortality ; pathology ; Lymphatic Metastasis ; Male ; Middle Aged ; Neoplasm Staging ; Prognosis ; Survival Rate
5.Expression of miR-140-5p and prediction of its target gene in human mesenchymal stem cells during adipogenic differentiation.
Tao WANG ; Rui-Qiao YAN ; Jun CAO ; Ling-Ling CAO ; Xuan-Pu ZHANG ; Xing-Nuan LI ; Ping WU ; Xiao-Ou ZHOU ; Jian-Fang WU ; Xiao-Yuan XU
Journal of Southern Medical University 2016;37(2):199-203
OBJECTIVETo screen the differentially expressed miRNAs and their target genes in adipogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs) to better understand the mechanism for regulating the balance between osteoblast and adipocyte differentiation.
METHODSCultured hMSCs were induced for adipogenic differentiation, and at 0, 7, 14, and 21 days of induction, the cells were examined for miRNA and mRNA expression profiles using miRNA chip and transcriptome sequencing (RNA-seq) techniques. Correlation analysis was carried out for the miRNAs and mRNAs of potential interest. The databases including TargetScan, PicTar and miRanda were used to predict the target genes of the differentially expressed miRNA.
RESULTSThe expression of miR-140-5p was down-regulated and leukemia inhibitory factor receptor (LIFR) expression increased progressively during adipogenic differentiation of hMSCs, showing a negative correlation between them. Target gene prediction using the 3 databases identified LIFR as the target gene of miR-140-5p.
CONCLUSIONmiRNA-140-5p may play an important role by regulating its target gene LIFR during adipogenic differentiation of hMSCs.
Adipocytes ; cytology ; Adipogenesis ; Cell Differentiation ; Cells, Cultured ; Down-Regulation ; Humans ; Leukemia Inhibitory Factor Receptor alpha Subunit ; metabolism ; Mesenchymal Stromal Cells ; cytology ; MicroRNAs ; genetics ; Oligonucleotide Array Sequence Analysis ; Osteoblasts ; cytology ; RNA, Messenger ; Transcriptome
6.'s experience in treating dry eye syndrome with fire needling.
Jian-Hong LI ; Wen-Jia RUAN ; Qiao-Lin LI ; Kun LIU ; Ying-Fei OU ; Guo-Hua LIN
Chinese Acupuncture & Moxibustion 2019;39(9):983-986
Professor - is the representative inheritor of fire needling therapy. He proposed that the clinical application of fire needling is mainly based on pattern differentiation according to eight priciples, as well as in coordination with the theories of meridians and - organs. He believes that dry eye syndrome can be treated from the aspects of deficiency of liver and kidney, heart deficiency, and and deficiency. Through deeply digging the connotation of ancient classics, and combined his clinical practice using fire needling, professor created a unique therapy that select "Neichengqi"as the main acupoint combined with Xialeidian, Dagukong (EX-UE 5), Xiaogukong (EX-UE 6) and Shaoze (SI 1) in the treatment of dry eye syndrome. In this paper, from the etiology and pathogenesis of dry eye syndrome and therapeutic characteristics of fire needling, the thoughts of syndrome differentiation and characteristics of acupoints selection of professor were analyzed by a typical case, and it is expect to enrich the clinical treatment plan for dry eye syndrome.
Acupuncture Points
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Acupuncture Therapy
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Dry Eye Syndromes
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therapy
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Humans
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Meridians
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Vascular Surgical Procedures
7. Effect of pimecrolimus on interleukin-33 level in mice with atopic dermatitis and asthma
Xueying LI ; Feng WANG ; Zhaoqing HAN ; Jian′ou QIAO
Chinese Journal of Postgraduates of Medicine 2019;42(11):999-1005
Objective:
To explore the potential mechanism of pimecrolimus in airway remodeling of atopic dermatitis (AD) and asthma mice.
Methods:
Female BALB/c mice were randomly divided into three groups: control group, atopic dermatitis group and pimecrolimus treatment group. The cell in bronchoalveolar lavage fluid (BALF) was calculated by Ray-Jimsa staining. The structural changes in lung tissue and skin tissue were observed by hematoxylin-eosin (HE) staining, and the level of interleukin (IL)-33, IL-5, IL-13 in serum was detected by enzyme-linked immunosorbent assay (ELISA). The expressions of type 1 collagen (Col1) in the airway of mice were detected by RT-PCR and Western blotting. And western blotting was also used to determine the activation of mitogen-activated protein kinase P38 (P38MAPK) and mitogen-and stress-activated protein kinase 1 (MSK1) in the lungs of mice.
Results:
Compared with those in the control group, the total number of leukocytes in bronchoalveolar lavage fluid (BALF) and the expression levels of IL-33, IL-13 and IL-5 in serum of atopic dermatitis group were significantly higher, and the difference was statistically significant (
8.β -Glucan Improves Protective Qi Status in Adults with Protective Qi Deficiency-A Randomized, Placebo-Controlled, and Double-Blinded Trial.
Jun-Rong WU ; Hao-Jie CHENG ; Jian-Pin SHI ; Wei-Dong YIN ; Jun WANG ; Xuan-Qiao OU ; Jin-Li CHEN ; Ira BERNSTEIN ; Mark LEVY ; Rolando MADDELA ; Robert SINNOTT ; Jun-Qiang TIAN
Chinese journal of integrative medicine 2022;28(5):394-402
OBJECTIVE:
To test the hypothesis that β -glucan enhances protective qi (PQi), an important Chinese medicine (CM) concept which stipulates that a protective force circulates throughout the body surface and works as the first line of defense against "external pernicious influences".
METHODS:
A total of 138 participants with PQi deficiency (PQD) were randomized to receive β -glucan (200 mg daily) or placebo for 12 weeks. Participants' PQi status was assessed every 2 weeks via conventional diagnosis and a standardized protocol from which a PQD severity and risk score was derived. Indices of participants' immune and general health status were also monitored, including upper respiratory tract infection (URTI), saliva secretory IgA (sIgA), and self-reported measures of physical and mental health (PROMIS).
RESULTS:
PQi status was not significantly different between the β -glucan and placebo treatment groups at baseline but improved significantly in the β -glucan (vs. placebo) group in a time-dependent manner. The intergroup differences [95% confidence interval (CI)] in severity score (scale: 1-5), risk score (scale: 0-1), and proportion of PQD participants (%) at finish line was 0.49 (0.35-0.62), 0.48 (0.35-0.61), and 0.36 (0.25-0.47), respectively. Additionally, β -glucan improved URTI symptom (scale: 1-9) and PROMIS physical (scale: 16.2-67.7) and mental (scale: 21.2-67.6) scores by a magnitude (95% CI) of 1.0 (0.21-1.86), 5.7 (2.33-9.07), and 3.0 (20.37-6.37), respectively, over placebo.
CONCLUSIONS
β -glucan ameliorates PQi in PQD individuals. By using stringent evidence-based methodologies, our study demonstrated that Western medicine-derived remedies, such as β -glucan, can be employed to advance CM therapeutics. (ClinicalTrial.Gov registry: NCT03782974).
Adult
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Double-Blind Method
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Humans
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Qi
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Risk Factors
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Self Report
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beta-Glucans/therapeutic use*
9.Does Percutaneous Coronary Intervention on Off Days have an Effect on Long-term Prognosis in Patients with Coronary Artery Disease in China?
Ru LIU ; Li Jian GAO ; Ou XU ; Ce ZHANG ; Si Da JIA ; Shu Bin QIAO ; Yue Jin YANG ; Run Lin GAO ; Bo XU ; Jin Qing YUAN
Biomedical and Environmental Sciences 2021;34(5):387-394
10.Consensus on clinical management of tumor-induced osteomalacia.
Yan JIANG ; Xiang LI ; Li HUO ; Yong LIU ; Wei LYU ; Lian ZHOU ; Wei YU ; Huan-Wen WU ; Xiao-Ping XING ; Mei LI ; Ou WANG ; Yue CHI ; Rui-Zhi JIAJUE ; Yu PEI ; Jian-Min LIU ; Jian-Ming BA ; Qiao ZHANG ; Zhi-Feng SHENG ; Zhen-Lin ZHANG ; Jia-Jun ZHAO ; Salvatore MINISOLA ; Wei-Bo XIA
Chinese Medical Journal 2021;134(11):1264-1266