Objective:To Construct a prokaryotic expression vector of cystatin C(Cys C)to provide a basis of purify Cys C protein produced by the expression system.Methods:Total RNA was isolated from HL-60 cells,and human Cys C gene was amplified with RT-PCR.The cDNA fragment was cloned into pET32a(+)vector,which was confirmed with sequencing and electrophoresis.Results:The result of electrophoresis showed that Cys C had been cloned into pET32a(+)vector,and the result of.DNA sequence analysis also showed the cloned Cys C gene sequence was completely correspondent to GeneBank data.Conclusion:The prokaryotic expression vector of cystatin C(Cys C)was obtained,providing a basis of purification of Cys C protein produced by the expression system.

Objective: To study the effect of acute hypoxia with simulated high altitude on the expression of orexins in rats hypothalamus. Methods: Rats were exposed to different high altitude(3 000 m,(4 000) m,5 000 m and 6 000 m for 8 h daily) in a hypobaric chamber.The expression of orexins in rat hypothalamus was determined by immunohistochemistry and the pathology changes of rat hypothalamus was observed with transelectricity. Results: The number of orexin-A immunoreactive positive cells in hypothalamus decreased obviously with hypoxia for 1 day(P

Objective To study the differences of electrolytes,C-reactive protein in different type,gender and recur- rent of febrile convulsion.Methods 174 cases of children febrile convulsion were collected.We detected the electro- lytes,C-reactive protein respectively and analyzed their relationship.Results In children febrile convulsion,the lev- els of the electrolytes,C-reactive protein had nothing to do with the type,gender and recurrent of the febrile convul- sion.Conclusion We should treat routinely the children who suffered from febrile convulsion without considering the type,gender and recurrent.

This study was aimed to further explore theory of small intestinal diseases and its principle of syndrome differentiation and treatment and lay the base for the clinical study, through the clinical research on the treatment of small intestinal diseases. Through the clinical survey among 584 patients, using Traditional Chinese Medicine (TCM) Clinical Multidimensional Relational Query System, which was established by the research on the clinical individual diagnosis and treatment evaluation system on the prevention and treatment of diseases, to conduct the multidimen-sional relational analysis on small intestinal diseases' disease-symptoms-syndromes-therapy-medicines-effects. Using data mining and statistical methods, such as WEKA, Business Objects, SPSS to mine and analyze the syn-dromes and symptoms. The results showed that commonly-used TCM methods on treatment of small intestinal dis-eases, therapeutic principles of main syndromes of small intestinal diseases were summarized. It was concluded that through scale-free network diagram analysis on the commonly-used treatment of small intestinal diseases, the main treatment methods included promotion the flow of qi, relieving pain, tonifying spleen, removing dampness, clearing away heat, mildly regulating cold and heat. And the treatment methods of dispersing cold, invigorating blood circula-tion and removing blood stasis, nourishing yin, moistening and loosening bowels, abductively dispersing food stagna-tion were also used. It corresponds with the results of clinical research.

Thyroidectomy provides rapid solution of hyperthyroidism and the goal of perioperative management is to make patient as close as possible to euthyroidism clinically and biochemically before surgery.It is recommended to target at the thyroid hormone synthetic,secretory,and peripheral pathway with concurrent treatment to correct any latent decompensation of normal homeostatic mechanisms.

Objective; To test the effect of fosinopril and valsartan on cardiomyocte apoptosis and expression of Bcl - 2 and Bax in myocardium of ischemia - reperfusion (I - R) rat model. Methods; SD rats were randomly divided into four groups as follows: Sham - operation group. Ischemia - reperfusion group . Fosinopril group and Valsartan group. The TdT - mediated dUTP nick end labeling (TUNEL) method was used to detect apoptotic myocytes in different groups. Bcl - 2 and Bax expression in myocardium were analyzed by immunohistochemical technique. Results ; The number of apoptotic cardiomytocytes in Ischemia 30min - reperfusion 48 hours group were significantly more (247. 2?25.9), than those in Ischemia 30min - reperfusion 4 hours group (P

Aim To explore the antitumor active metabolites of marine-derived Streptomyces flavorectus Z4-007. Methods The separation procedure was guided by a flow cytometric bioassay using tsFT210 cells to examine cell cycle inhibitory activity, and various column chromatography using silica gel, Sephadex LH-20, and ODS were employed for the isolation and purification of bioactive compounds. Chemical structures were investigated by spectroscopic methods and biological activities were evaluated by flow cytometry using mouse cancer tsFT210 cells. Results Four bioactive compounds were isolated from the fermentation broth of Streptomyces flavorectus Z4-007, one of them had been identified as 1-(2,4-Dihydroxy-3,5-dimethyl-phenyl)-hexa- (E,E)-2,4-dien-1-one (1) and the other three compounds were considered to be peptide, Compound Ⅰ inhibited the cell-cycle of tsFT210 cells at the G_0/G_1 phase at higher concentrations, while at the lower concentrations compound Ⅰ inhibited the cell-cycle mainly at the G_2/M phase, accompanied with induction of apoptosis in the tsFT210 cells.Conclusion Compound Ⅰ was isolated from the metabolites of the genus Streptomyces for the first time and provided as a new cell-cycle inhibitor.

Objective:To assess clinical value of grading scale for intracerebral hemorrhage(ICH).Methods:By using the grading scale for ICH suggested by Hemphill and colleagues, 205 patients with acute ICH presenting to our department during 2000～2001 were studied retrospectively.Results:Thirty-mortality of ICH patient increased steadily with ICH Score.Conclusion:The grading scale for ICH is a simple amd reliable method of ICH score,worthy of clinical application.

Objective To set a RFID(Radio Frequency Identification) system for Gold Immunochromatographic Assay by using RFID-reading module,RFID tags and the master-MCU.Methods Radio frequency technology is characterized by the automatic identification and large dada-storage capacity.Electronic tags were packed with Gold Immunochromatographic Assay(GICA) strips and such data were stored in electronic tags as strip information,the test results,the identity of the tested,the test date,etc.The information were transmitted to PC through the RS-232 serial port,thus realizing the rapid and accurate recording and management of data.Results The application of RFID guaranteed the proper use of Gold Immunochromatographic Assay strips and improved the efficiency of sample management and data storage.Conclusion The application of RFID to Gold Immunochromatographic Assay can be used in general investigation of epidemic situation,biomedical detection and other relevant fields.

Objective To study the adhesion and proliferation of rabbit bone marrow mesenchymal stem cells(BMSCs) on the HA/TCP bone graft scaffold.Methods BMSCs were harvested from the iliac bone and obtained by using adhesive culture method.BMSCs were induced with osteogenic medium,at the 7th day,the cells were stained by the calcium cobalt method to show the activities of alkaline phosphatase(ALP).At the 10th day,the mineralized nodules of osteoblasts stained with chinalizarin.Otherwise BMSCs were induced with adipogenic medium,and at the 21st day the cells stained with oil red O.We cultured the BMSCs on the surface of a matrix scaffold with osteogenic medium.The morphologic characters were checked by light microscopy,fluorescence microscopy and scanning electronic microscopy,and the proliferation of BMSCs were assayed using the MTT test.Results At the 7th day after osteoblastic induction,ALP was strongly positive,and at the 10th day,mineralized nodules stained with chinalizarin were jacinth.At the 21th day after adipogenic induction,the adipocytes were stained with oil red O.BMSCs grew well around or in pores of the HA/TCP bone graft scaffold and could be seen using both fluorescence microscopy and scanning electron microscopy.The MTT test assay showed that the HA/TCP couldn't inhibit the proliferation of BMSCs.Conclusion BMSCs have a good biocompatibility with a HA/TCP bone graft scaffold.