1.Study on Antagonism of GSH and Taurine to Acute Oxidative Damage Induced by Mercury
Jiaming YU ; Zhaofa XU ; Zhongwei YIN
Journal of Environment and Health 1993;0(01):-
Objective To study the antagonism of glutathione(GSH) and taurine on acute oxidative damage caused by mercury(Hg). Methods 32 Wistar rats were randomly divided into four groups. The rats of control group were given 0.9% saline by subcutaneous injections. The rats in mercuric chloride (HgCl2) group were subcutaneously injected with 2.5 mg/kg HgCl2. Rats of the other two groups were pretreated with 3 mmol/kg GSH and 4 mmol/kg taurine, respectively and two hours later injected subcutaneously with 2.5 mg/kg HgCl2. Urine creatine and mercury contents were determined; mercury level, contents of GSH, MDA and GSH-Px in liver and kidney were evaluated. Results Compared with the control, urine mercury level, level of mercury in liver and in the renal cortex, contents of GSH, MDA and GSH-Px in kidney in the group treated with 2.5 mg/kg HgCl2 were significantly increased. In the rats pretreated with GSH and taurine, contents of MDA in kidney were significantly decreased compared with those treated with mercury only. The levels of GSH-Px in kidney in the group pretreated with GSH and taurine were significantly higher than that not only in the mercury group but also in the control. Compared with the mercury group, levels of mercury in urine and liver in GSH pretreated group were distinctly reduced. Conclusion Pretreated with GSH and taurine have certain protection for the acute oxidative damage caused by mercury.
2.A Meta-analysis of the accuracy of interferon-γrelease assays in differentiating intestinal tuberculosis from Crohn's disease in Asia
Hui XU ; Yue LI ; Jiaming QIAN
Chinese Journal of Internal Medicine 2016;55(7):535-540
Objective This meta-analysis assessed the value of interferon-γ release assays (IGRAs) in the differential diagnosis of intestinal tuberculosis (ITB) from Crohn's disease (CD).Methods Systematic search without language restriction was conducted in the main computerized databases until June 2015.Studies that have evaluated the performance of IGRAs (QuantiFERON-TB Gold or T-SPOT.TB) in distinguishing ITB from CD were eligible.Main outcome measures included sensitivity and specificity.Area under the curve (AUC) of the summary receiver operating characteristic (sROC) curve was used to evaluate the accuracy of IGRAs.Results Twelve studies (all from Asia) were finally included.The pooled sensitivity and specificity of IGRAs for the differential diagnosis of ITB from CD were 82.8% (95%CI 78.4%-86.6%) and 86.7% (95% CI 83.2%-89.6%) respectively.The positive likelihood ratio (PLR) and negative likelihood ratio (NLR) were 6.870 (95% CI 5.345-8.830) and 0.171 (95% CI 0.105-0.279).The diagnostic odds ratio was 44.030 (95% CI 27.964-69.325).And the AUC of sROC was 0.939.Conclusions IGRAs have a high sensitivity and specificity for the diagnosis of ITB,and specificity is consistent from study to study.IGRAs may be considered as a supplementary method in the differential diagnosis of ITB from CD.
3.Determination of the Serum Concentration of Buspirone by HPLC in Rabbits
Weijuan ZHAO ; Jiaming BIAN ; Jingfeng XU
China Pharmacy 2001;0(10):-
OBJECTIVE:To establish a HPLC method for determination of the concentration of Buspirone in serum.MET_ HODS:The chromatographic system consisted of ZORBA SB-C 18 column and mobile phase of methanol-acetonitrile-water-0.1mol/L NaAC buffer solution(pH4.5)(10∶38∶51∶1),with a detection wavelength of237nm,and the content was calcu?lated by peak area internal standard method.RESULTS:The linear range of Buspirone was1~80ng/ml,r=0.9979.CONCL_ USION:This method is simple and accurate for determination of Buspirone in serum.
4.Experimental Study of Percutaneous Absorption of Neostigmine Cream in Vitro
Weijuan ZHAO ; Jingfeng XU ; Jiaming BIAN
China Pharmacy 1991;0(06):-
OBJECTIVE:To study the in vitro percutaneous absorption of neostigmine cream.METHODS:Using isolated mouse skin as permeation barrier,the influence of different concentrations of azone on percutaneous permeation of neostigmine was studied.RESULTS:Azone could obviously enhance the percutaneous permeation of neostigmine,and the accumulative permeation quantity of neostigmine cream containing2%and5%azone increased7.17%and11.87%respectively compared with that of cream without azone.CONCLUSION:The percutaneous permeability of neostigmine cream is satisfactory and the best concentration of azone in enhancing permeation seems to be5%.
5.The Preventive and Therapeutic Effect of Keshu on Acute Alcoholism Mice
Ming ZENG ; Juan XU ; Jinping WANG ; Jiaming BIAN ; Jingfeng XU
China Pharmacy 2001;0(09):-
OBJECTIVE:To observe the preventive and therapeutic effect of Keshu on acute alcoholism mice.METHODS:Acute alcoholism mice model was established,and randomly divided mice into model group and high,middle,low dosage of Keshu group;the tolerance time,lasting time and the concentration of alcohol in blood were compared among each group.RE-SULTS:Compared with the model group,the tolerance time has been prolonged,the lasting time has been shortened and the blood concentration of alcohol has been lowered in Keshu group.CONCLUSION:Keshu has an obvious preventive and thera-peutic effect on acute alcoholism,the preventive effect of which outweighs the therapeutic effect.
6.Value of detection for serum VEGF levels in clinical diagnosis and curative effect on colon cancer
Jiaming ZHANG ; Jingning XU ; Yan LI ; Ben WEN ; Chaoyang XIE
Cancer Research and Clinic 2013;(4):230-233
Objective To investigate the clinical value of serum VEGF levels in the diagnosis and treatment of colon cancer.Methods Serum VEGF was detected by ELISA,and CEA and CA199 concentration were detected by CLEIA in 66 patients with colon cancer,55 patients with colon benign diseases and 50 health persons.The value of individual and joint detection for VEGF in colon cancer was evaluated.Analysis had been done on relationships between serum VEGF and pathology,treatment effects and prognosis.Results The levels of serum VEGF in colon cancer group [(318.5±148.6) ng/L] were significantly higher than those in control group [(125.7±49.4) ng/L] and benign colon diseases [(136.9±52.6) ng/L] (t =8.830,8.805,all P < 0.01).There was a positive correlation between serum levels of VEGF and depth of tumor size,tumor invasion,lymph node metastasis and TNM stage (P < 0.01).The susceptibilities of VEGF,CEA,CA199 were 61%,45 %,53 %.The sensitivity of detection was improved to 86 % when the combined detection of VEGF,CEA and CA199 (x2 =11.237,P < 0.01).The serum levels of VEGF in patients with colon cancer was significantly decreased after treatment in the 3,7,10 day compared with that before operation [(272.3±88.1),(236.8±77.4),(173.1±59.9) vs (318.5±148.6) ng/L,t=2.173,P < 0.05; t =3.961,P < 0.01; t=7.464,P < 0.01],respectively.Conclusion The VEGF was related to the onset and progression and metastasis of colon cancer.It has clinical significancy for diagnosis of colon cancer and judgment of curative effect and prognosis.
7.Research progress of the central mechanism of acupuncture-moxibustion for simple obesity
Jiaming XING ; Xueyan SHENG ; Xuan XU ; Zhongting ZHAO ; Xingke YAN
Journal of Acupuncture and Tuina Science 2016;14(6):438-442
Obesity often results when regular caloric intake exceeds the energy expenditure. Hypothalamus is the center that regulates feeding and energy balance inside the body, especially the lateral hypothalamic area (LHA) and ventromedial nucleus (VMN). Acupuncture-moxibustion has achieved significant progress in treating simple obesity, and the relevant studies have revealed the central action mechanism underlying the treatment. By reviewing the literatures associated with the mechanism studies on acupuncture-moxibustion for simple obesity, this article is going to provide clinical and scientific guidance for the treatment of simple obesity with acupuncture-moxibustion.
8.Application of HC visual laryngoscope in emergency tracheal intubation for severe craniocerebral trauma patients with basilar fracture
Jiaming ZHANG ; Jinyu XU ; Guorong HUANG ; Jun SHI ; Dongdong JI ; Jiong GENG ; Qingmiao DONG
Journal of Regional Anatomy and Operative Surgery 2017;26(6):442-445
Objective To assess the clinical application values of HC visual laryngoscope in emergency tracheal intubation for severe craniocerebral trauma patients with basilar fracture.Methods Retrospective analysis was used to review 60 patients of severe traumatic brain injury with basilar fracture in emergency resuscitation room who needed the hospital emergency tracheal intubation.There were 30 patients operated with visual HC laryngoscope (the observation group), and the other 30 patients underwent common direct laryngoscope (the control group).The index of the degree of exposure,intubation time,success rate of intubation were compared between the two groups.ResultsThere were 28 cases (93.33%) of Cormark-Lehane grading Ⅰ to Ⅱ in observation group,which was significantly higher than 20 cases (66.67%) in the control group(P< 0.05).The average intubation time in observation group was (34.3±6.7) seconds,and it was shorter than (44.5±5.7) seconds in the control group (P<0.05).In observation group,26 cases (86.67%) were of one-time successful intubation,which was higher than 19 cases (63.33%) in the control group,and the difference was statistically significant(P<0.05).ConclusionHC visual laryngoscope in emergency tracheal intubation for severe craniocerebral trauma patients with basilar fracture can improve the success rate of intubation,shorten the intubation time,reduce adverse reactions,and it is worthy of clinical application.
9.Expression and location of hypoxia inducible factor-1α and -2α in the remnant kidney of 5/6 nephrectomy rats
Xiaofang YU ; Xiaoqiang DING ; Jiaming ZHU ; Yi FANG ; Jianzhou ZOU ; Xunhui XU ; Suhua JIANG
Chinese Journal of Nephrology 2010;26(9):689-695
Objective To investigate the location and expression of hypoxia inducible factor (HIF) subunits in the remnant kidney of 5/6 nephrectomy rats. Methods Remnant kidneys were produced in adult male SD rats by 5/6 nephrectomy. The renal function and histopathological changes were evaluated at week 1, 2, 4, 6, 8 and 12 after operation. Tissues of remnant kidneys were collected to detect the location and expression of HIF-1α and HIF-2α by immunohistochemistry staining and Western blotting. The mRNA levels of HIF targeted genes vascular endothelial growth factor (VEGF) and heme oxygenase-1 (HO-1) were determined by RTPCR. Results (1) 5/6 nephrectomy rats underwent one week of acute renal failure at first[Scr (122.8±22.1) μmol/L] and then developed compensative chronic renal failure [(66.0±3.7)-(66.4±8.4) μmol/L], but the level of Scr increased quickly after week 6 [(66.4±8.4)-(127.8±22.7) μmol/L],concomitantly with progressive tubulointerstitial fibrosis in remnant kidney cortex. (2) In cortex, HIF-1α was expressed only in the atrophic and dilated tubular cells while HIF-2α was located in endothelial, interstitial fibroblasts, and vascular smooth muscle cells. The semiquantitative results of imunohistochemistry and Western blotting revealed that HIF-1α and HIF-2α were both gradually up-regulated during the early stage of remnant kidney, peaked at week 4 and 6, and then gradually down-regulated. (3) The mRNA levels of HIF targeted genes VEGF and HO-1 transiently peeked at week 4 and 6, and then decreased gradually. Conclusions The increased stabilization of HIF-αprotein and transcription of HIF targeted genes at the early stage of this model is a compensation reaction towards hypoxia. The mechanism of decreased expression of HIF-α at the end stage of chronic kidney disease deserves further investigation.
10.Application of modified three-step fluorescence in situ hybridization in prenatal diagnosis
Yan ZENG ; Jiaming FAN ; Lifang ZHANG ; Feiyan QIAN ; Ping XU ; Weijun JIANG
Chinese Journal of Laboratory Medicine 2014;37(4):266-269
Objective To investigate a simple and efficient fluorescence in situ hybridization method for prenatal diagnosis.Methods Thirty-six cases of chorionic villus samples were hybridized with probes 18/X/Y,13/21 by using traditional cuhure methods and modified three-step methods in controlled experimentation during 2012 to 2013.The hybridization rate and fluorescence signals were analyzed.Results A total of 72 hybrid zone were detected.Probe 21/13 hybridization rate and fluorescence signals of three-step modified methods were higher than that of traditional methods(99.72% ±0.42% vs 85.90% ±4.15%,t =20.4,P <0.01; 2.58 ±0.50 vs 1.52 ± 0.55,t =7.53,P <0.01).Probe 18/X/Y has the same hybridization rate and fluorescence signals between three-step modified methods and traditional methods (99.57% ±0.53% vs99.70% ±0.42%,t=1.30,P>0.05; 2.22±0.42 vs2.36±0.48,t=1.57,P>0.05).The coincidence rate of two methods was 100% (36/36).Conclusion The modified fluorescence in situ hybridization three-step methods in the study was simple,rapid,effective and environment-friendly.At the same time,it has some defects such as the signals of 18/X/Y was not concentrated enough.Further exploration is needed.