Objective To explore the effects of directive game in preoperative visit of preschool children with strabismus.Methods 109 children were randomly assigned into the control group and the experimental group.The control group was given conventional preoperative interview,based on the general preoperative visit,the experimental group was given preoperative visit-playing directive game.The nursing effect was compared be4wecn two groups.Results Induction compliance scores of the experimental group were obviously higher than those of the control group.Preoperative anxiety scores of the experimental group were obviously lower than those of the control group.Restlessness during recovery of the experimental group was obviously lower than that of the control group.Conclusions The application of directive game can reduce preoperative anxiety of the children with strabismus,and make children cooperate better with the induction of anesthesia.

Colorectal cancer (CRC) belongs to multiple diseases of traditional Chinese medicine (TCM).CRC can exhibit sophisticated clinical manifestations and various TCM syndromes (Zheng).Modem studies have revealed that syndromes (Zheng) of CRC are related to pathological characteristics,cell types,staging and prognosis.Surgery,chemotherapy and other modem treatments can influence the manifestations of syndromes (Zheng) of CRC.Modern TCM doctors have extensively studied the biomedical basis of syndromes (Zheng) of CRC,including accumulation of damp-heat (Shi-Re-Yun-Jie),blood stasis (Yu-Xue-Nei-Zu),spleen (Pi) deficiency (Pi-Qi-Kui-Xu),deficiency of qi and blood (Qi-Xue-Liang-Xu),deficiency of yin of liver (Gan) and kidney (Shen) (Gan-Shen-Yin-Xu) and other syndromes (Zheng).These investigations may contribute to the objective and standardization study of syndromes (Zheng) in CRC.

Objective:To investigate the effect of circ_0084927 as a molecular sponge adsorbing miR-623 to target and regulate EZH2 gene on immune escape in breast cancer (BC) cells.Methods:Bioinformatics websites were used to predict the circ_0084927/miR-623/EZH2 regulatory axis, which was then validated with the aid of dual luciferase reporter assay. BC cells were co-cultured with CD8+ T cells and theflow cytometry assays were performed to detect apoptotic rate and the proportion of T cells in the co-transfected microenvironment.Results:The expression level of EZH2 and circ_0084927 were significantly upregulated in BC patient tissue samples compared to paracancerous tissue, while miR-623 expression was dramatically diminished ( P<0.05) . circ_0084927 can be used as an effective diagnostic index of BC (AUC= 0.813 8, P<0.000 1) . Compared with apoptosis rate of CD8+ T cells (34.17±3.06) and percentage of T cells (26.55±2.83) , circ_0084927 inhibition caused a decrease in the apoptosis rate of CD8+ T cells (22.37±2.05) and an increase in the proportion of CD8+ T cells (31.88±3.03) in the co-transfected environment, but this effect was partiallycounteractedby overexpression of EZH2. miR-623 inhibited CD8+ T cell apoptosis. Circ_0084927 attenuated the inhibitory effect of miR-623 on immune escape (both P<0.05) . Conclusion:circ_0084927 regulates the miR-623/EZH2 axis to promote immune escape of BC cells, suggesting targeting circ_0084927 may be a potential way to improve the efficacy of immunotherapy for BC.

Objective To observe the effect of Renshen(ginseng)-Yuzhu(fragrant solomonseal rhizome)on inflammatory factors and inflammasomes in depression rats,and to explore the antidepressant mechanism of Renshen-Yuzhu.Methods Fifty male SD rats were divided into the blank group,model group,fluoxetine group(2.1 mg/kg),Renshen-Yuzhu low-dose group(1.89 g/kg),and Renshen-Yuzhu high-dose group(5.67 g/kg),with ten rats in each group.Except for the blank group,the rats in the other groups were treated with chronic unpredictable mild stress to establish a depression rat model.On the second day after the end of modeling,the rats in each group were given the corresponding drugs once daily for 14 days.After modeling and dosing,body weight,forced swimming immobility time,and sucrose preference rate were measured.After dosing,hematoxylin-eosin staining was used to detect neuronal damage in the cerebral cortex,enzyme-linked immunosorbent assay was used to detect the contents of interleukin-4(IL-4),interleukin-23(IL-23),and interleukin-27(IL-27)in cortex,real-time PCR was used to detect the mRNA expression of interleukin-24(IL-24)in cortex,and the protein expressions of nucleotide-binding oligomerization domain-like receptor protein 1(NLRP1),absent in melanoma 2(AIM2),and nucleotide-binding oligomerization domain-like receptor protein 4(NLRC4)were detected by Western blotting.Results After dosing,compared with the blank group,the body weight of the model group decreased,the sucrose preference rate decreased,the swimming immobility time was prolonged,the neuronal tissue in cortex was destroyed,the content of IL-4 in cortex decreased,the contents of IL-23 and IL-27 in cortex increased,and the protein expressions of NLRP1,AIM2 and NLRC4 in cortex increased(P＜0.01).Compared with the model group,the body weight of rats in each administration group increased,the sucrose preference rate increased,the swimming immobility time was shortened,the damage of neuronal tissues in cortex improved,the content of IL-4 in cortex increased,the contents of IL-23 and IL-27 in cortex decreased,and the protein expressions of NLRP 1,AIM2 and NLRC4 in cortex decreased(P＜0.05).Conclusion Renshen-Yuzhu couplet medicines can improve the depressive-like behavior and exert antidepressant effect in chronic stress rats,and its mechanism may be related to the inhibition of NLRP 1,NLRC4,AIM2 inflammasome activation and its mediated inflammatory response in cortex,reducing pro-inflammatory cytokines,and increasing the level of antiinflammatory cytokines.

Objective To explore the effects of spot-inspection form on self-care ability of postoperative patients with diabetic retinopathy .Methods By the random sampling method , we chose 86 postoperative patients with diabetic retinopathy .All the patients were randomly assigned into two groups-the control group (n=42) and the experimental group (n=44).The control group was given healthy guidance and medication directions.The experimental group was given extra education of spot-inspection form.The Exercise of Self-care Agency Seale ( ESCA ) and self-designed questionnaire were used to assess the postoperative patients with diabetic retinopathy on the second day after operation , one month after operation and three months after operation.Results The second day after the operation and one month after operation , the self-care ability of postoperative patients with diabetic retinopathy had no significant differences between patients in the two groups (P>0.05).Three months after the operation , the scores of self-care, self-concept, self-care responsibility, health knowledge level and self-care ability in the experimental group were (32.18 ±4.99), (21.98 ±2.71), (20.50 ±2.17), (53.73 ±6.73) and (128.39 ±14.00), respectively.Those scores in the control group were (30.60 ±5.04), (20.64 ±3.15), (19.36 ±2.30), (50.83 ±7.39) and (121.43 ±15.53).The scores in the experimental group were significantly higher than those in the control group ( t=2.109,2.367, 1.903,2.184, respectively;P<0.05), except for the self-nursing skills (P<0.05).Conclusions The spot-inspection form applied to postoperative patients with diabetic retinopathy has practical significance to improve the self-care ability, promote the rehabilitation of patients and improve the quality of life .

Objective To explore the effects of training evaluation cycle mode applied for enhancement of core competence of nurses in Ophthalmic Operating Room .Methods A total of 60 nurses were assessed evaluation of training requirements , training plans , training implementation and training results by using training evaluation cycle mode , and promptly revised scheme based on the assessment results .The investigation had been carried out for nurses′professional knowledge , skills and comprehensive ability , satisfaction of nurses , doctors and patients in 2011 , 2012 and 2013 .Results Professional knowledge and skills , comprehensive response ability gained (84.63 ±4.69),(83.27 ±3.79) and (88.67 ±4.66),which were all statistically higher than those in 2011 and 2012 (F=20.24, 14.30, 32.45;P<0.01).The satisfactions of nurses, doctors and patients had no statistical difference between 2011 and 2012 (P >0.05), but had statistical difference between 2012 and 2013 (P<0.05).Conclusions Training evaluation cycle mode is feasible to improve the core competence of nurses in Ophthalmology Operation Room .

Objective:To explore the effect of emotion control game based on Snyder hope theory on anxiety of children and their parents.Methods:From June to December 2021, 92 preschool children who received operation and their parents in the Second Affiliated Hospital of Wenzhou Medical University were selected as the study subject. The subjects were divided into two groups according to the time of admission, among which 46 children and their parents admitted from June to September 2021 were taken as the control group, with routine nursing. A total of 46 children and their parents admitted from October to December 2021 were taken as the study group, and emotion control game based on Snyder hope theory was implemented on the basis of the control group. The scores of the Chinese version of the modified Yale Perioperative Anxiety Scale (Cm-YPAS) one day before operation and at the time of entering the Operating Room were compared between the two groups of children, and the scores of the Generalized Anxiety Disorder-7 (GAD-7) and the Newcastle Satisfaction with Nursing Scale (NSNS) of the parents of preschool children one day before operation and at the time of discharge of the two groups were compared.Results:During the intervention, there were 3 cases of dropout children in the study group and two cases of dropout children in the control group, and 43 and 44 children completed the study respectively. The Cm-YPAS scores of children in the two groups at the time of entering the Operating Room were statistically higher than those of children one day before operation; When entering the Operating Room, the Cm-YPAS score of children in the study group was lower than that in the control group, with a statistically significant difference ( P<0.05) . The GAD-7 scores of parents of children in the study group were lower than those in the control group one day before operation and at the time of discharge, and the difference was statistically significant ( P<0.05) . At the time of discharge, the NSNS scores of parents in the study group were higher than those in the control group, with a statistically significant difference ( P<0.05) . Conclusions:In preoperative nursing, emotion control game based on Snyder hope theory for preschool children can effectively reduce their preoperative anxiety, and the anxiety of their parents during perioperative period, and improve parents' satisfaction with nursing.

Objective To investigate the self-perceived burden (SPB) of diabetic retinopathy (DR) patients after diabetic retinopathy and its influencing factors, and analyze the correlation between them. Methods Self-designed general questionnaire and self-rating questionnaires including SPB scale, etc. were used in the survey. All survey information was imported into Excel database. SPSS 18.0 statistical software was used for the descriptive analysis, single-factor analysis and multiple-factor analysis. Results The average SPB score of DR patients after surgery was (28.22±7.11) points, and was in a mild level. Working condition, children condition, the time span since diagnosis and the knowledge of vitrectomy all had positive effect on SPB. Conclusions The SPB of DR patients after surgery is at a mild level. The SPB of patients after DR surgery is mainly influenced by their working condition, children condition, the time span since diagnosis and the knowledge of vitrectomy.

Objective : investigate the correlation between the ossification of the styloid hyoid ligament and Bell’s facial paralysis and provide a reference for clinical diagnosis and treatment. Methods: A case of ossification of the bilateral stylohyoid ligament with Bell's facial palsy caused by ossification of the bilateral stylohyoid ligament was diagnosed by clinical manifestations, differential diagnosis and imaging examination. The surgical plan was determined, and combined surgical resection of the ossified area of the styloid hyoid ligament and the greater horn of the hyoid was performed. Postoperative cefoxitin sodium anti-inflammatory treatment, methylprednisolone hormone treatment, acyclovir antiviral treatment, mecobalamin nutritional neurotherapy, and the relevant literature were analyzed. Results: The patient experienced pain when swallowing before surgery, disappearance of right frontal ridges, incomplete eyelid closure, and ptosis of mouth corners. An MRI scan of the brain excluded intracranial space-occupying lesions and resulted in the diagnosis of Bell’s facial paralysis. High-resolution CT of the styloid process confirmed ossification of the styloid hyoid ligament. Styloid process shortening and partial hyoid resection were performed under general anesthesia. Half a month after discharge, the symptoms of sore throat and pain in swallowing disappeared, facial nerve function recovered well, right eyelid closure function recovered well, and right mouth droop improved. The facial nerve function basically returned to normal after 1 month of follow-up. A review of the relevant literature showed that ossification of the stylohyoid ligament to form pseudojoint dilation can locally stimulate the peripheral facial nerve and lead to facial paralysis symptoms. Conclusion Ossification of the styloid hyoid ligament is usually characterized by pharyngeal pain, which can be confirmed by imaging examination. Ossification of the styloid hyoid ligament with facial paralysis is rare in the clinic, so it is necessary to make a clear diagnosis and treat the symptoms.

A new definition of metabolic dysfunction-associated fatty liver disease (MAFLD) has recently been proposed. We aim to examine the associations of MAFLD, particularly its discordance from non-alcoholic fatty liver disease (NAFLD), with the progression of elevated brachial-ankle pulse wave velocity (baPWV) and albuminuria in a community-based study sample in Shanghai, China. After 4.3 years of follow-up, 778 participants developed elevated baPWV and 499 developed albuminuria. In comparison with the non-MAFLD group, the multivariable adjusted odds ratio (OR) of MAFLD group for new-onset elevated baPWV was 1.25 (95% confidence interval (CI) 1.01-1.55) and 1.35 (95% CI 1.07-1.70) for albuminuria. Participants without NAFLD but diagnosed according to MAFLD definition were associated with higher risk of incident albuminuria (OR 1.77; 95% CI 1.07-2.94). Patients with MAFLD with high value of hepamet fibrosis score or poor-controlled diabetes had higher risk of elevated baPWV or albuminuria. In conclusion, MAFLD was associated with new-onset elevated baPWV and albuminuria independently of body mass index, waist circumference, and hip circumference. Individuals without NAFLD but diagnosed as MAFLD had high risk of albuminuria, supporting that MAFLD criteria would be practical for the evaluation of long-term risk of subclinical atherosclerosis among fatty liver patients.
Humans ; Pulse Wave Analysis ; Albuminuria ; Ankle Brachial Index ; Non-alcoholic Fatty Liver Disease/diagnosis* ; Vascular Stiffness ; Prospective Studies ; Risk Factors ; China/epidemiology*