In this study collagen was applied locally for the treatment of burn wound in the rat. Twenty rats weighing 18-22g were made into 7% [1 degree burn model and divided into two groups: collagen group and control group. After burn, collagen (0.4ml, once daily) was smeared on the rat wound in the treatment group, whereas saline (0.4ml, once daily) in the control group. It was found that collagen can significantly accelerate burn wound healing by comparing the wound healing time and histological changes.

Objective Delve into the applied value of CDFI to diagnose deep vein thrombosis(DVT)in lower limbs.Methods Analyze the diagnosis of 65 chronic cases of DVT with a two-dimensional CDFI,and contrast it with the operation,X-ray angiography and clinical features.Results 36 acute cases of DVT;29 chronic cases of DVT;38 cases of lesion in left lower hmb(58.5%);21 in right lower limb(32.3%);and 6 in both lower limbs(9.2%).In the chronic cases,11 have formed collateral branch circulation.Conclusion CDFI has a safe and rapid effect on vein illness in lower limbs and can show the state of blood streams and the condition around the veins.It Can explain the unknown swollen in limbs.Realizing DVT in early stage has good value for its occurring,expanding and treating.

Objective To investigate the echocardiographic determination of uremic patients with myocardial weight and in the evaluation of cardiac function in patients with uremia heart the significance of the damage.Methods 70 cases of uremic patients and 30 normal physical were examined by routine echocardiography,cardiac measurements through the menu of uremic patients with myocardial weight and heart function.The patient group and the normal control group were compared.Results 70 patients with uremic echocardiography resuits of the analysis showed,respectively,12.8%and 84.3%existence of left ventricular systolic dysfunction and left ventricular diastolic dysfunction.left ventricular mass in patients with significant increase are significantly higher than those in healthy control group(P＜0.01).Conclusion Uremic patients left ventricular diastolic dysfunction and left ventricular hypertrophy most of echocardiography is the detection of cardiac damage in patients with uremia practical,sensitive,non-invasive examination will be able to clinical diagnosis and treatment provide guidance in the clinical widely used.

Objective: To observe the effect of Tuina (Chinese therapeutic massage) on creatine kinase (CK), mitochondrial Ca2+ concentration, and ultrastructure of skeletal muscle in delayed onset muscle soreness (DOMS) model rats.Methods: A total of 130 healthy male Sprague-Dawley rats were randomly divided into a blank group, an exercise control group, a pre-exercise Tuina group, and a post-exercise Tuina group. According to the time points for sample collection, the exercise control group was divided into a 0 h exercise control group, a 24 h exercise control group, a 48 h exercise control group, and a 72 h exercise control group; the pre-exercise Tuina group was further divided into a 0 h pre-exercise Tuina group, a 24 h pre-exercise Tuina group, a 48 h pre-exercise Tuina group, and a 72 h pre-exercise Tuina group; and the post-exercise Tuina group was divided into a 0 h post-exercise Tuina group, a 24 h post-exercise Tuina group, a 48 h post-exercise Tuina group, and a 72 h post-exercise Tuina group. Rats in all groups except for the blank group received DOMS modeling. Professionals performed Nie-Pinching manipulation and finger Nian-Twisting manipulation on the lower limbs of the rats. The samples were collected at 0 h, 24 h, 48 h, or 72 h after exhaustive exercise for each pre-exercise Tuina group. The samples were collected at 0 h, 24 h, 48 h, or 72 h after Tuina for each post-exercise Tuina group. The changes in serum CK, skeletal muscle mitochondrial Ca2+ concentration, and Ca2+-adenosine triphosphatase (ATPase) were determined. The ultrastructure changes of skeletal muscles in each group were observed by a transmission electron microscope. Results: The electron microscope showed that compared with the exercise control group, the skeletal muscle structures of the pre-exercise Tuina group and the post-exercise Tuina group were significantly improved, and the overall performance of skeletal muscle in the pre-exercise Tuina group was more similar to that of the blank group. The level of serum CK in the pre-exercise Tuina group and the post-exercise Tuina group was significantly lower than that in the exercise control group (P<0.01). The Ca2+ concentration of skeletal muscle in the 24 h, 48 h, and 72 h pre-exercise Tuina groups was lower than that in the post-exercise Tuina group at the same time point (P<0.01). The Ca2+-ATPase concentration of skeletal muscle in the 24 h and 72 h pre-exercise Tuina groups was lower than that in the post-exercise Tuina group at the same time point (P<0.05).Conclusion: Tuina effectively prevents muscle damage caused by heavy exercise and long-term exercise, which may be related to the increase of skeletal muscle Ca2+-ATPase activity and mitochondrial Ca2+ transport.

Objective To explore the relationship between impulsiveness and risky decision-making among college students with pathological internet use ( PIU),as well as the moderating role of self-control. Methods Totally 542 undergraduates anonymously completed the Internet Addiction Test (IAT),Chinese Version of Barratt Impulsiveness Scale,Risk Preference Questionnaire,Self-Control Scale. Results ( 1) In loss frames,the scores of risky decision-making with high (20. 26±4. 83) and medium (20. 40±5. 53) level of impulsiveness among college students with PIU significantly were higher than those with low ( 18. 92 ± 5. 03) level of impulsiveness ( F(2,323)= 3. 79,P<0. 05,η2=0. 02). (2) There was a significant negative correlation between impulsiveness (60. 68±10. 40) and self-control (55. 56±9. 07) (r=-0. 60,P<0. 01). And there was a significant positive correlations between impulsiveness and risky decision-making in loss frames (20. 12±5. 26)(r=0. 13,P<0. 05). The risky decision-making in gain frames ( 17. 17±4. 38) was positively correlated with that in loss frames (r=0. 52,P<0. 01). (3) Self-control played a moderating role in the association between impulsiveness and risky decision-making in loss frames (β=0. 13,t=2. 31,P<0. 05). Conclusion In loss frames,impulsiveness is positively related with risky decision-making,and self-control plays a moderating role between impulsiveness and risky decision-making in loss frames among col-lege students with PIU.

OBJECTIVETo study the clinical presentations of Kennedy disease (KD) and compare the neurophysiological features between KD and amyotrophic lateral sclerosis(ALS).

METHODSNine patients with KD, 13 patients with ALS and 26 normal control subjects were recruited. The clinical presentations of KD were analyzed, and the results of nerve conduction studies and electromyography were compared among the 3 groups.

RESULTSThe rates of tongue atrophy and facial fasciculation were 100% and 88.9%, respectively, in the early course and mid-course of KD, sensory damages might be perceived. 2)The sural nerve sensory nerve action potential (SNAP) was not elicited in 56.3% of the patients with KD, and sural nerve SNAP amplitudes were significantly lower in KD (7.9. ± 3.4 µV) than in ALS patients (20.0 ± 5.2 µV) and normal control subjects (26.1 ± 16.8 µV) (P<0.05).

CONCLUSIONB The onset of clinical presentations mimicking motor neuron disease, appearance of tongue atrophy and facial fasciculation in the early and mid-course, and presence of sensory impairment with a decreased sural nerve SNAP amplitude may suggest the diagnosis of KD and should prompt a genetic test for KD.

Objective: To investigate the antibiotic resistance spectrum and genetic characteristics of multidrug-resistant Staphylococcus aureus(MDRSA) nasal isolate among primary school students, and to provide a scientific basis for the prevention and control of masal MDRSA resistance and the selection of clincal drugs in children. Methods: Antibiotic susceptibility experiments were performed on all SA isolates of 1 705 primary school students from 8 primary schools in Guangzhou selected by using multistage cluster stratified sampling method. MDRSA antibiotic susceptibility spectrum was analyzed, and the resistant, virulence and immune evasion cluster(IEC) genes detected by polymerase chain reaction(PCR). Results: The prevalence of MDRSA nasal carriage was 20.76%(354/1 705), and the proportion of multidrug resistance among SA isolates was 96.20%(354/368). The predominant resistant antibiotics of MDRSA isolates were penicillin(99.72%), erythromycin(96.33%), clindamycin(90.96%) and teicoplanin(90.11%). Notably, 240(67.80%, 240/354) MDRSA isolates were resistant to more than six antimicrobial categories. And the predominant detection rates of resistant genes were BlaZ(92.66%), Tet(M)(49.72%), virulence genes Tst(25.42%) and IEC genes Sak(92.09%), Hlb(61.58%). Conclusion We found high prevalence of nasal colonization MDRSA from healthy children. Moreover, MDRSA isolates has a high resistant rate to multiple antibiotics, and the proportion of resistant to ≥6 antimicrobial categories is high.

Objective: To investigate the contamination, antimicrobial resistance and virulence genes of S. aureus from toilets of primary schools in Guangzhou. Methods: The surface samples of toilets were collected from eight primary schools in Guangzhou from May to July 2016. The standard microbiological assays, disk diffusion methods and PCR technique were used for the isolation and identification, antimicrobial resistance and virulence genes of S. aureus . Results: The contamination rate of S. aureus and MRSA was 6.25% and 3.13%, respectively. There was significant difference in the contamination rate of S. aureus among different sampling sites ( χ 2=15.15, P <0.01) and the highest contamination rate was on the ground (15.00%).The most predominant antibiotic for S. aureus was penicillin (100.00%) and the proportions of resistant to teicoplanin, erythromycin,rifampicin, clindamycin and linezolid were more than 75.00%.The multidrug resistant rate of S. aureus was 85.00%.The detection rate of virulence genes of S. aureus was sea (50.00%), tst (30.00%), etb (15.00%), eta (10.00%), seb (10.00%) and pvl (5.00%), respectively. Conclusion The contamination rate of S. aureus from toilets of primary schools in Guangzhou is in a lower level among similar researches. However, the contamination of MRSA is serious, which accounts for half of S. aureus . In addition, S. aureus isolates show high multi-drug resistant rate and high detection rate of virulence genes.

Objective: To evaluate the effectiveness of varicella vaccine in varicella outbreaks and to analyze the influencing factors, and to provide a reference for making the targeted prevention and controlling measures. Methods: A total of 3 888 students with no history of varicella were selected from 2 schools with varicella outbreak in Guangdong Province in 2021, a retrospective cohort study was conducted by using questionnaire survey, rate ratio ( RR ) and vaccine effectiveness ( VE ) values were calculated and Logistic regression was uses to analyze the factors influencing the protective effect of varicella. Results: There were 138 confirmed cases of varicella among the participants. There was no significant sex difference in the vaccination rate( χ 2=1.36, P =0.51), but there was significant difference in the vaccinattion rate of different age groups( χ 2=555.82, P <0.01). The overall protective effect of VarV was 66.94%(95% CI =56.17%-77.71%), and the protective effect of 2 doses of vaccine( VE = 90.02% , 95% CI =83.13%-96.90%) was higher than that of 1 dose( VE =49.40%, 95% CI =32.36%-66.44%)( χ 2=24.93, P < 0.01 ). The high fever rates in the vaccinated and unvaccinated groups were 7.69% and 25.81%, with significant difference( χ 2= 6.29 , P <0.05). The rates of moderate and severe skin lesions of vaccinated and unvaccinated groups was 20.00% and 50.00%, respectively, and the difference was statistically significant( χ 2=11.32, P <0.01). The protective effects of varicella vaccine against high fever and moderate to severe rash were 70.19%(95% CI =42.11%-98.27%) and 60.00%(95% CI =38.15%-81.85%). Stratified analysis showed that there were significant differences in different years of vaccination( χ 2=37.87, P <0.05), while there were no significant differences in age of vaccination and vaccine manufacturer ( P >0.05). Conclusion Varicella vaccination can prevent chickenpox infection and reduce the severity of the disease. However, the efficacy of varicella vaccine was affected by vaccination years. It is recommended to improve the vaccination coverage of varicella vaccine to prevent the outbreak of the epidemic.

Neuromyelitis optica spectrum disorder (NMOSD) is a central nervous system inflammatory disease characterized by aquaporin-4 immunoglobulin G (AQP4-IgG). The core clinical manifestations include acute myelitis, optic neuritis and area postrema syndrome. AQP4-IgG-positive NMOSD patients have severe symptoms, frequent relapses, less complete recovery and early disability. In recent years, AQP4-IgG-positive NMOSD has gradually been recognized, but its exact mechanism is still unclear. This paper reviews the recent advance in pathogenesis of AQP4-IgG positive NMOSD.