Objective:This paper aimed to investigate citizens' cognition and willingness to living organ dona-tion and to provide advice for the development of living organ donation.Methods:A sample of 1500 ordinary citi-zens in Shanghai was randomly selected and a self-administered questionnaire survey was conducted anonymously to investigate their cognition and willingness to living organ donation.Results:The citizens' awareness rate of liv-ing organs was not high;citizens obtained the information mainly through the network,television,newspapers and other mainstream media;citizens' s willingness rate of living organ donation was 14.6％,and the willingness wasin-fluenced by various factors.Conclusion:To promote citizens' correct understanding of living organ donation re-quires various efforts of governments,societies,families,hospitals and schools.

Objective:To discuss the ethical issues of living organ donation in China systematically and comprehensively,from the perspective of society,institution,family,individual and other level.Methods:Literature research and expert interviews were used as main research methods,supplemented by group discussion,in-depth interviews and other methods.Results:In the development of living organ donation in China,from the social level,the relevant laws and regulations,civic education and supporting measures had problems.From the institutional level,hospital human organ transplant ethics committee also had some deficiencies in staff composition,review capacity and supervision.On the individual level,donors and receptors of living organ donation and related medical personnel also had their own ethical dilemma.Conclusion:It is beneficial to fully promote living organ donation and fully realize the purpose of living organ transplantation for the benefit of mankind through solving the ethical issues from social,institutional and individual levels.

Objective:To systematically and comprehensively expound the features and problems of ethics com-mittee for human organ transplantation in the ethical review work of living organ transplantation. Methods:We took interviews with insiders,literature research as the main research methods,supplemented by group discussion and other methods. Results:The ethical review of living organ transplantation has the characteristics of high require-ments of promptness,the need of protection for the rights of life and health for both parties,the complicated prepara-tion work,the need of weighing the risk and benefit of both parties at the same time and the lack of final judgment. In terms of the problems existing in the ethical review of living organ transplantation,it mainly had that the ethical review of living organ transplantation lacked supervision and guidance,the feasibility and scientificity of ethical re-view mode need to be improved,and the self - construction of ethics committee for human organ transplantation was insufficient. Conclusion:The ethics review of human organ transplant ethics committee should follow the principle of prudence,and improve the standardization and normalization of ethical review of human organ transplantation. Re-gional human organ transplant ethical review center should better conduct the ethical review work of living organ transplantation.

Space-occupying brain edema is a life-threatening complication in patients with cerebellar infarction, which occurs in 17-54% of the patients. The level of consciousness should be monitored closely and the changes of CT/MRI should be followed up for cerebellar infarction patients with a higher risk of deterioration. Ventriculostomy/extraventricular drainage and suboccipital decompressive craniectomy should be performed in space-occupying cerebellar infarction patients with clinical deterioration.

December 2019 witnessed the outbreak of COVID-19 in Wuhan and spread of the epidemic across the country. As a provincial designated hospital for critical patients, the First Affiliated Hospital of Zhejiang University responded rapidly since then by advocating the four-concentration principles, namely " concentrating patients, experts, resources and treatment" . In its rescue of critical patients, the hospital formulated comprehensive emergency plans, optimized hospital-wide resources, effectively arranged rescue spacing, established medical echelons, and implemented multi-disciplinary strategy. These efforts ensured efficient rescue and treatment, achieving a cure rate up to 98.7% of such patients, with no deaths.

Objective To investigate the possible associations of two polymorphisms (5-HTTLPR and STin2VNTR)of the serotonin transporter gene with alcohol use disorders (AUD).Methods 281 AUD cases (AUDIT score≥ 10) and 277 healthy controls (AUDIT score ≤5) were recruited in this study.All participants were genotyped using the PCR technique.Results The frequency of the L-allele of the 5-HTTLPR was 39.01％,and the 10-allele of STin2VNTR was 8.42％ in this population,the allele frequencies of both polymorphisms were consistent with Asian normal populations.No significant association was observed between 5-HTTLPR and AUD,but the genotypic and allele frequencies of the STin2VNTR were significant different between two groups even after Bonferroni adjustment,the 12 repeat allele of the STin2VNTR was significantly associated with the risk effect for AUD.Haplotype analysis for those two polymorphisms revealed no association between 4 haplotype combinations and AUD.Conclusion There is no relationship between 5-HTTLPR and AUD.The STin2VNTR polymorphism of 5-HTT may play a role in the pathogenesis of AUD.

OBJECTIVE To prepare Neuritic acid oral emulsion ，to optimize its formulation and preparation technology ，and to investigate its stability. METHODS Neuritic acid oral emulsion was prepared by mechanical method. On the basis of single factor experiment ，the appearance ，centrifugal stability ，centrifugal stability constant （Ke）and particle size of the emulsion as indexes，the formulation was optimized by orthogonal design ，taking the dosage of oleic acid ，octylphenol polyoxyethylene ether-10 and propylene glycol as factors ，the preparation technology was optimized by taking emulsification temperature ，shear time，pressure of high-pressure homogenization and cycle times of high-pressure homogenization as factors. The content of neuritic acid was determined by high performance liquid chromatography. The stability of Neuritic acid oral emulsion was investigated by high temperature test ，accelerated test and long-term test. RESULTS The optimal formulation and preparation technology were as follows：neuritic acid of 1 g，oleic acid of 5％ ，octylphenol polyoxyethylene ether- 10 of 4％ ，propylene glycol of 2％ ， emulsification temperature of 60 ℃ ，shear time of 2 min，homogenization pressure of 40 MPa and cycle times of twice. After three experiments ，the average particle size of Neuritic acid oral emulsion was 158.05 nm（RSD＝1.58％，n＝3），the average Ke was 0.39（RSD＝1.49％，n＝3），and the appearance was uniform milky white ，there was no stratification. The results of high temperature test showed that Neuritic acid oral emulsion was prone to stratification in high temperature environment ，and the content of neuritic acid increased. The results of accelerated test and long-term test showed that there was no significant change in the appearance or the content of neuritic acid when Neuritic acid oral emulsion was placed at room temperature for 6 months. CONCLUSIONS The formulation and preparation technology are stable and feasible ，and can be used for the preparation of Neuritic acid oral emulsion. Neuritic acid oral emulsion should not be placed in high temperature environment. It has good stability at room temperature for 6 months.

BACKGROUND: The new emerging avian influenza A H7N9 virus, causing severe human infection with a mortality rate of around 41%. This study aims to provide a novel treatment option for the prevention and control of H7N9. METHODS: H7 hemagglutinin (HA)-specific B cells were isolated from peripheral blood plasma cells of the patients previously infected by H7N9 in Jiangsu Province, China. The human monoclonal antibodies (mAbs) were generated by amplification and cloning of these HA-specific B cells. First, all human mAbs were screened for binding activity by enzyme-linked immunosorbent assay. Then, those mAbs, exhibiting potent affinity to recognize H7 HAs were further evaluated by hemagglutination-inhibiting (HAI) and microneutralization in vitro assays. Finally, the lead mAb candidate was selected and tested against the lethal challenge of the H7N9 virus using murine models. RESULTS: The mAb 6-137 was able to recognize a panel of H7 HAs with high affinity but not HA of other subtypes, including H1N1 and H3N2. The mAb 6-137 can efficiently inhibit the HA activity in the inactivated H7N9 virus and neutralize 100 tissue culture infectious dose 50 (TCID50) of H7N9 virus (influenza A/Nanjing/1/2013) in vitro, with neutralizing activity as low as 78 ng/mL. In addition, the mAb 6-137 protected the mice against the lethal challenge of H7N9 prophylactically and therapeutically. CONCLUSION The mAb 6-137 could be an effective antibody as a prophylactic or therapeutic biological treatment for the H7N9 exposure or infection.
Animals ; Antibodies, Monoclonal/therapeutic use* ; Antibodies, Neutralizing/therapeutic use* ; Antibodies, Viral ; Hemagglutinins ; Humans ; Influenza A Virus, H1N1 Subtype ; Influenza A Virus, H3N2 Subtype ; Influenza A Virus, H7N9 Subtype ; Influenza Vaccines ; Influenza in Birds ; Influenza, Human/prevention & control* ; Mice