Objective To explore the feasibility of thoracic sympathectomy under video mediastinoscopy (VM) for palmar hyperhidrosis. Methods Operations on 55 patients with palmar hyperhidrosis were performed under general anesthesia with double lumen endotracheal tube placement. The patients were maintained in the 30?～45? semi-sitting position, with abduction of both arms to 90?. The VM entered the thoracic cavity via the third intercostal space on the anterior axillary line. The T 2～T 4 sympathetic ganglions were resected by electrocautery. Results Hyperhidrosis disappeared postoperatively in all the cases. The temperature of patients' both hands increased by 1.5～3 ℃,with their both hands becoming dry and warm. The operation time was 20～40 min (mean, 30 min) and the hospital stay was 3～7 d (mean, 4 d). No severe complications occurred. Mild subcutaneous emphysema along with pneumothorax was found in 3 cases and compensatory hyperhidrosis in the trunk happened in 5 cases. Conclusions Thoracic sympathectomy under VM is less invasive and simpler than that under video-assisted thoracoscopic surgery (VATS) in the treatment of palmar hyperhidrosis, being a feasible alternative.

ObjectiveTo investigate the clinical appli cation of ultrasound in Peyronie disease. MethodsSixty-nine patients with Peyronie disease were examined by conventional ultrasound, and compared with pathology. ResultsUltrasonographic imaging showed the size and morphological of Peyronie plague,and located the position of the plagues.The ultrasound figures of plagues directly related to pathological components and case history,and they were roughly divided into twe types:hypoechoic type and hyperechoic type.ConclusionsUltrasound examination is the best and reliable method in choice for Peyronie plagues and evaluation of the therapeutic effects.

Objective: To establish the quality standard of Fushukang Granules (Radix Astragali, Radix Angelicae sinensis, Rhizoma Chuanxiong, Radix Paeoniae Alba, etc.). Methods: TLC was used in qualitative identification of the Radix Astragali, Radix Angelicae Sinensis, Rhizoma Chuanxiong, Radix Paeoniase Alba. And TLC-scanning was used for assay of astragaloside I in the preparation. Results: Radix Astragali, Radix Angelicae Sinensis, Rhizoma Chuanxiong, Radix Paeoniae Alba could be detected by TLC. Astragaloside I had a good linear relationship within the range of 1.001～5.005?g(r=0.9998). The average recovery was 92.46%, and the RSD was 2.82%, respectively. Conclusion: The method established is stable and reliable. It can be used for quality control of the preparation.

Objective To investigate the gene polymorphism of inhibitory killer cell immunoglobulin- like receptor (iKIR) in patients with inflammatory bowel disease (IBD) and whether the iKIR gene polymorphisms were associated with IBD.Methods Peripheral blood DNA samples were isolated from 100 patients with ulcerative colitis (UC),52 patients with Crohn's disease (CD) and 106 randomly ethnically matched healthy controls.The iKIR gene polymorphisms were analyzed by sequence specific primer polymerase chain reproduction (PCR-SSP).Phenotypic frequency and gene frequency of iKIR gene were calculated,and differences were compared between IBD patients and healthy controls.Results iKIR genes (including KIR2DL1,KIR2DL2,KIR2DL3,KIR2DL4,KIR2DL5,KIR3DL1,KIR3DL2, KIR3DL3) were found to be present in all subjects at different levels.Interestingly,phenotypic frequencies of KIR2DL1 and KIR2DL3 were significantly lower in UC patients than those in healthy controls (P = 0.001),while phenotypic frequencies of KIR2DL2,KIR2DL4,KIR2DL5,KIR3DL1, KIR3DL2 and KIR3DL3 were no difference between UC patients and healthy controls (P>0.05).The phenotype frequency of KIR2DL1 was significantly decreased in CD patients compared with healthy controls (P = 0.007),while phenotypic frequencies of other iKIR were observed to be no significant change between CD patients and healthy controls (P>0.05 ).Conclusions The KIR2DL1 and KIR2DL3 gene phenotype frequencies are decreased in UC patients,which suggests that these gene polymorphisms are associated with the susceptibility of UC,and the polymorphism of KIR2DL1 gene is involved in the susceptibility of CD.

Objective To investigate the quantitative expression of mRNA levels of human NOV and WT1 genes in renal cell carcinoma ( RCC). Methods Using quantitative real-time RT-PCR analysis, we quantified NOV and WT1 mRNA levels in the samples from 57 patients (40 men and 17 women; mean age,65 years) with RCC. Of them,39 cases had T1 stage tumor, 13 had T2 stage and 5 had T3a stage by UICC pathologic staging;and 14 cases had G1 tumor,23 had G2 and 20 had G3 by Fuhrman grading. The expression levels of NOV and WT1 genes,and their association were analyzed. Results The median level of NOV mRNA expression was significantly lower in RCC ( 1. 17) than in normal kidney tissue (4. 32, P

We presented a new method for vessel segmentation and vascular structure recognition for coronary angiographic images. During vessel segmentation, a new vessel function was proposed to attain vessel feature map. Then the region growing algorithm was implemented with an automatic selection of seed point, extraction of main vessel branch, and vessel detail repairing. In the algorithm of vascular structure recognition, a fuzzy operator was used, which can detect the structures of vascular segments, bifurcations, crosses, and tips. The experimental results indicated that there was about 5 percent larger vessel region which was extracted by the proposed segmentation method than that by the simple region growing algorithm, and several thinner vessels were resumed from the lower gray region. The results also indicated that the fuzzy operator could correctly infer the simulative and real vessel structure with 100% and 90.59% correctness rate on the average, respectively.
Algorithms ; Coronary Angiography ; Humans ; Radiographic Image Enhancement ; methods ; X-Rays

Objective To analyze the epidemiological characteristics,clinical manifestations and treatment of hallucinogenic boletus poisoning patients,and to provide reference for clinical diagnosis and treatment.Methods We retrospectively analyzed the epidemiological data,clinical manifestations,laboratory tests,treatment and outcomes of 36 patients with hallucinogenic boletus poisoning in our hospital from 2015 to 2022,and identified the species of mushrooms responsible for poisoning the patients.Results Of the 36 cases,32 cases were poisoned from June to September,24 cases were poisoned in towns and 12 cases in the countryside.Twenty-three patients were poisoned after eating multiple meals.Thirty-five patients ate Boletus lanmao identified by using genetic fingerprint and 7 ate Lanmaoa asiatica by using genetic sequence comparison.The incubation period of poisoning ranged from 1 to 48 h,with a median(quartile)of 19.0(12.0,24.8)h.All 36 cases had hallucinatory symptoms.Other symptoms were agitation and irritability in 21 cases,consciousness disorders in 29 cases,motor disorders in 21 cases,speech disorders in 19 cases and gastrointestinal symptoms in 18 cases.Laboratory tests showed that 16 patients had increased white blood cells(WBC)and percentage of neutrophils(NEUT%)and decreased percentage of lymphocyte(LY%).After fluid resuscitation combined with sedation and antipsychotic medication,all patients were cured and discharged from the hospital,of which 33 cases were discharged within 7 days.Conclusion Hallucinogenic boletus mushroom poisoning mainly occurs in summer and fall,the poisoned mushrooms are mainly Lanmaoa asiatica mushrooms,the incidence of poisoning is high in patients eating multiple meals,and the typical symptom is hallucination,and the patients can be cured through active symptomatic supportive treatment.

A new proposed data mining technique, support vector machine (SVM), is used to predict the degree of malignancy in brain glioma. Based on statistical learning theory, SVM realizes the principle of data dependent structure risk minimization, so it can depress the overfitting with better generalization performance, since the prediction in medical diagnosis often deals with a small sample. SVM based rule induction algorithm is implemented in comparison with other data mining techniques such as artificial neural networks, rule induction algorithm and fuzzy rule extraction algorithm based on fuzzy max-min neural networks (FRE-FMMNN) proposed recently. Computation results by 10 fold cross validation method show that SVM can get higher prediction accuracy than artificial neural networks and FRE-FMMNN, which implies SVM can get higher accuracy and more reliability. On the whole data sets, SVM gets one rule with the classification accuracy of 89.29%, while FRE-FMMNN gets two rules of 84. 64%, in which the rule got by SVM is of quantity relation and contains more information than the two rules by FRE-FMMNN. All the above show SVM is a potential algorithm for the medical diagnosis such as the prediction of the degree of malignancy in brain glioma.
Algorithms ; Artificial Intelligence ; Brain Neoplasms ; pathology ; Diagnosis, Computer-Assisted ; methods ; Female ; Glioma ; pathology ; Humans ; Magnetic Resonance Imaging ; Male ; Models, Statistical ; Neural Networks (Computer) ; Predictive Value of Tests

Objective:To study the clinical effect of sacrospinous ligament fixation (SSLF) and traditional vaginal hysterectomy on pelvic organ prolapse (POP) .Methods:A retrospective analysis was performed on 68 patients with POP of degree II-IV admittedl from Jan. 2017 to Dec. 2019. Among them, 33 patients were treated with SSLF (observation group) and 35 patients were treated with vaginal total hysterectomy (control group). Intraoperative blood loss, operative time, postoperative indwelling catheter and average length of hospital stay were compared between the two groups. The patients were followed up for 6 months, and the scores of pelvic floor dysfunction questionnaire-20 (PFDI-20) and sexual quality questionnaire -12 (PISQ-12) were used to evaluate the subjective satisfaction degree of postoperative recovery.Results:In the observation group, the intraoperative blood loss (173.94±52.14) ml, postoperative indurating catheter time (2.72±0.45) d and average length of hospital stay (7.09±0.63) d were observed. There were statistically significant differences in intraoperative blood loss (228.86±53.40) ml, postoperative induration time (4.54±0.61) d and mean hospital stay (9.22±0.81) d in the control group ( P<0.05). There was no significant difference in the operation time between the observation group (99.57±9.50) min and the control group (101.06±8.64) min, ( P>0.05). The improvement of PFDI-20 and PISQ-12 in both groups was significant before and after treatment. The PISQ-12 score of the observation group was higher than that of the control group 6 months after surgery, and the difference was statistically significant ( P <0. 05). There was no significant difference in PFDI-20 score between the two groups ( P>0.05). There was statistical significance in the positions of pop-Q indicators in the two surgical methods ( P < 0.05) . Conclusions:SSLF with uterus preservation and total vaginal hysterectomy are both effective in treatment of moderate and severe POP. However, SSLF with uterus preservation has less intraoperative blood loss, and the postoperative recovery is significantly better than that with total vaginal hysterectomy. In addition, it satisfies patients’ desire to preserve uterus, improves the postoperative sexual life quality, which is worthy of promotion.

Objective:To analyze the correlation between the expressions of human epidermal growth factor receptor 2(HER2)and carbohydrate antigen 153(CA153)and the technical parameter of acoustic palpation tissue imaging quantification(VTIQ)in patients with breast cancer.Methods:A total of 80 female patients with breast cancer admitted to The Third People's Hospital of Hefei from May 2018 to May 2020 were selected,including 14 cases at WHO stage Ⅰ,22 cases at WHO stage Ⅱ,31 cases at WHO stage Ⅲ and 13 cases at WHO stage Ⅳ.Another 53 female patients with benign breast diseases who were treated during the same period were selected as controls.At first,all patients underwent routine ultrasound examination,and then they entered the ultrasound VTIQ imaging mode to obtain the mean value of shear wave velocity(SWV).An immunohistochemistry was used to detect HER2 expressions in breast tissues,and Roche E411 electrochemiluminescence immunoassay analyzer was used to detect serum CA153 levels of them.Pearson method was used to analyze the correlation between serum CA153 levels and SWV mean values in patients with breast cancer.Results:Compared with benign patients,the SWV mean value of VTIQ technical parameter,serum CA153 level and HRR2 positive expression rate in patients with breast cancer were significantly higher,and the difference was statistically significant(F=39.107,78.353,P<0.05),respectively.Compared with patients at stages Ⅰ + Ⅱ of breast cancer,the SWV mean value of VTIQ technical parameter,serum CA153 level and HRR2 positive expression rate of patients at stages Ⅲ and Ⅳ of breast cancer significantly increased(t=2.685,3.556,8.326,10.455,P<0.05),respectively.Compared with patients at stage Ⅲ of breast cancer,the SWV mean value of VTIQ technical parameters,serum CA153 level and HRR2 positive expression rate of patients at Ⅳ stage of breast cancer were significantly higher(t=4.632,8.659,P<0.05),respectively.Compared with the SWV mean value of patients with HER2 negative expression of breast cancer,that of patients with HER2 positive expression of breast cancer was significantly higher(x2=59.751,P<0.05).There was a positive correlation between serum CA153 levels and SWV mean values in patients with breast cancer(r=0.501,P<0.05).Conclusion:The SWV mean value of VTIQ parameters is closely related to the expression levels of biomarkers HER2 and CA153 in patients with breast cancer.