Objective To investigate the changes of disease activity and clinical significance in the course of treatment in patients with SLE.Method 286 cases of SLE were reviewed and compared the changes of SLEDAI scores in different disease duration.Results The SLEDAI scores of patients whose first treatment courses less than 1 month and 1 to 3 months were significantly lower than those patients whose were 4 to 6 months and more than 6 months. After treatment for 2 months to 3 years, the SLEDAI scores were not correlated with cumulated dosage of corticosteroids.Conclusions For the patients of short first treatment course, the treatment could relieve SLE disease activity rapidly and effectively to some extent; while for the patients whose first treatment courses were relatively long ,the relif of disease activity was relatively slow. After treatment for 2 to 3 months, the disease of SLE patients was more active than other periods, and it was inclined to produce visceral damage. As mentioned above ,we should pay attention to this phenomenon.

Objective To investigate the effect of modified Taohe Chengqi Decoction(TCD)and its separated prescriptions on tissue plasminogen activator(tPA)and plasminogen activator inhibitor-1(PAI-1)content in human umbilical vein endothelial cells(HUVEC)with diabetic damage.Methods Purified HUVEC were divided into 10 groups: normal group,model group,rosiglitazone group,high-,middle-and low-dose modified TCD groups,blood-activating prescription group,heat-clearing and purgation prescription group,Qi-strengthening and yin-nourishing group,and TCD group.The serum containing herbal medicine was prepared through seropharmacological method,and was used for co-culture of HUVEC with high glucose and high insulin in vitro.The tPA and PAI contents in the HUVEC culture of different groups were detected.Results The content of tPA was increased in blood-activating prescription group and rosiglitazone group(P0.05).The PAI-1 content in the model group was higher than that in the normal group(P0.05).Conclusion The effect of modified TCD on improving the function of endothelial cells is probably related to the regulatory effect of blood-activating,heat-clearing and purgation herbs on PAI-1.

Objective To investigate the conrelation between neutrophils lymphocytes ratio (NLR) and disease activity of ulcerative colitis (UC).Methods Case-control study was used to compare NLR differences of 82 active UC patients,45 inactive UC patients,and 254 healthy controls.The multivariate analysis was applied to analyze the correlation between the NLR,erythrocyte sedimentation rate (ESR),Creactive protein (CRP),white blood cells (WBCs),and UC activity.The sensitivity and specificity of NLR to identify UC activity was evaluated.Results NLR of active UC group was 2.45 ± 1.22,which was significantly higher than that of inactive UC group and healthy control group,their NLR were 1.92 ± 0.68 and 1.83 ±0.75,respectively (H =9.991,P =0.007).The multivariate analysis showed that only CRP was correlated with UC activity (OR =1.396,95％ CI:1.086 ～ 1.795,P =0.009).Receiver operating characteristic (ROC) curve analysis showed that the optimum NLR cut-off point for UC activity was 2.23,the sensitivity and specificity were 55.82％ and 62.75％,respectively.Conclusions NLR has a certain reference value for the evaluation of UC activity,but it cannot be independent as a clinical index to evaluate the UC activity.

OBJECTIVETo investigate the reconstruction of 1/4 defect on upper-lip vermilion with a lower-lip vermilion compound tissue flap pedicled at oral commissure.

METHORDSAt the first stage, the lower lip mucosal flap pedicled by inferior labial artery was transposed to reconstruct the defect on upper lip vermilion and tubercle. The defect at the donor site was closed directly. At the second stage, the flap pedicle was cut off and revised.

RESULTS6 patients were treated with satisfactory aesthetic results. All the flaps survived completely. The oral commissure kept normal with no obvious scar at the donor sites.

CONCLUSIONSThe modified crosslip vermilion flap pedicled at oral commissure has the advantages of avoiding inconvenience in feeding, speaking and cleaning. The procedure is simple with available blood supply. Both aesthetic and functional results are satisfactory.

Arteries ; Esthetics ; Humans ; Lip ; surgery ; Mouth Mucosa ; transplantation ; Surgical Flaps ; blood supply ; Transplant Donor Site ; surgery

PurposeHeroin addiction is a chronic and recurrent functional brain disease, there are some functional changes in specific brain regions, but the network character remains unclear. The aim of this paper is to explore the network character of brain resting-state functional network in heroin addicts, to identify the potential neuromechanism of heroin addiction from the perspective of brain network.Materials and Methods Thirty heroin addicts (HA group) and twenty-nine healthy controls (control group) underwent resting-state functional MRI scanning using GE 3.0T MRI scanner. The brain functional networks were constructed based on graph theory, the small-world properties and node properties were calculated and compared between the two groups, the correlation between the total dosage of heroin and node degree was analyzed.Results Compared with control group, the small world characteristics of HA group was altered with statistically significant difference (P<0.05, corrected by false discovery rate); the node degrees in orbit frontal regions increased, while those in occipital brain regions decreased (P<0.05, corrected by false discovery rate). No correlation was found in HA group between node degree and the total dosage of heroin.Conclusion These results suggest that topology of functional brain networks were altered in heroin addicts which tends to random networks; increased motivational driving to the salience of drug and decreased visuospatial attention in heroin addicts may provide a strategy for identifying the neuromechanism of heroin addiction.

Objective To investigate the effects of different abstinent duration on brain functional network of heroin addicts at resting state.Methods Sixteen heroin addicts during protracted abstinence for 11-13 months (PA12 group) and twenty heroin addicts during protracted abstinence for 5-7 months (PA6 group) were recruited in the resting-state functional MRI study.Graph theoretical methods were applied to construct topological organization of whole brain network and nodes betweenness of the networks in all subjects,and the between-group differences were analyzed.The correlation of the node betweenness with the abstinence duration was conducted.Results There was no significant difference (P＞0.05) in the small world characteristic (γ≈1,λ》1) between two groups.Compared with the PA6 group,the PA12 group demonstrated significantly decreased nodal betweenness in regions of left parahippocampal gyrus,left precentral gyrus,and significantly enhanced nodal betweenness in regions of the left cuneus,left temporal and right middle occipital gyrus (all P＜0.05).Moreover,the betweenness of the left precentral gyrus (r =-0.52,P =0.001) and parahippocampal gyrus (r=-0.49,P=0.002) were negatively related with the abstinence duration,the betweenness of the right middle occipital was positively correlated with the abstinence duration (r=0.49,P=0.003).Conclusion The brain network small world topology of heroin addicts tend to be stable after 5-7 months of abstinence.Long-term abstinence may minimize the addicfs memory of drugs and potential drug seeking behavior,and recover addicts' visual spatial attention function.

Objective To analyze the clinical characteristics of new onset diffuse type ulcerative colitis.Methods From May 2007 to March 2012,clinical data of hospitalized patients diagnosed in the People's Hospital of Xinjiang Autonomous Region as new onset ulcerative colitis were retrospectively analyzed,and constituent ratios of nationality,gender,age at diagnosis,residential environment,severity of disease,clinical symptoms,results of laboratory examination,results of colonoscopic examination and results of histopathological examination were compared among the diffuse type,the type of proctosigmoiditis and the type of left-sided colitis.Normal distribution data were analyzed by one-way analysis of variance,skew distribution data and ordinal data were analyzed by Kruskal-Wallis H test,and count data were analyzed by chi-square test in the comparison among the three groups.Results A total of 67 patients with new-onset diffuse type ulcerative colitis were enrolled.The constituent ratios of Han nationality,Uygur nationality and Kazak nationality were 32.8％(22/67),58.2％(39/67),and 9.0％(6/67),respectively.The ratio of male to female was 1.48∶ 1.The age at diagnosis was (43.27 ± 16.91) years old.The ratio of city to countryside was 3.19∶ 1.To the severity of the disease,mild was more common,the proportion of mild,moderate and severe were 56.7％(38/67),20.9％(14/67) and 22.4％ (15/67),respectively.Abdominal pain,diarrhea and purulent stools were the predominant symptoms.Some patients might have fever and tenesmus,a few patients accompanied with abdominal distension,nausea,vomiting and peripheral arthritis.The percentage of increasesed erythrocyte sedimentation rate,hypersensitive Creation protein and plasmatic fibrinogen was 37.3％(25/67),44.8％(30/67) and 31.3％ (21/67),respectively.To compare with patients with proctosigmoiditis and left-sided colitis,the patients with new-onset diffuse type colitis had higher Mayo score and were more prone to develop abdominal pain,diarrhea,severe colitis,and were more common in Uygur nationality.There were statistical differences in abdominal pain,diarrhea,severity,ratio of Uygur nationality and Mayo score in pairwise comparison among the three groups (all P＜0.017).Conclusion Abdominal pain,diarrhea and Uygur nationality are more common in new onset diffuse ulcerative colitis in Xinjiang Region,with relatively high Mayo score and mild to moderate degree,while severity significantly increased compared to the type of proctosigmoiditis and the type of left-sided colitis.

[Objective] To investigate associations between the functional polymorphisms of signal transducer and activator of transcription 4 (STAT4) gene and unexplained recurrent spontaneous abortion (URSA) and between STAT4 protein expression and the genotypes of rs 10181656 locus.[Methods] PCR-restriction fragment length polymorphism was used to genotype rs 1 0181656 locus polymorphism in 332 URSA cases and 260 normal controls,in 86 URSA cases and 77 normal controls of which immunohistochemical technique was used to detect STAT4 protein expression.[Results] The frequencies of rs10181656 C/G were 36.45 ％,46.54％ in genotype C/C,46.99％,45.38％ in genotype C/G and 16.57％,8.08％ in genotype G/G between URSA patients and normal controls.They reached statistical difference (P ＜ 0.05).The carriers of rs 10181656 G allele increased the risk of URSA (OR =1.50,P ＜ 0.05).STAT4 protein expression in decidual tissues:①There was statistical difference in the STAT4 protein expression in decidual tissues between cases and controls (P ＜ 0.05).In URSA patients there was statistical difference in the STAT4 protein expression among genotype CC,CG and GG of rs10181656 locus (P ＜ 0.05).So was in normal controls (P ＜ 0.05).In genotype CC there was no difference in the STAT4 protein expression between cases and controls (P ＞ 0.05).Neither was In genotype CG and GG respectively (P all ＞ 0.05).[Conclusion] Functional polymorphisms of the rs10181656 locus could probably associate with the susceptibility of URSA via STAT4 protein expression increased by genotype G/G in maternal decidual tissue.

OBJECTIVE:To study the anti-oxidative effects of spirulina kinase (SPK) in vitro. METHODS:The methods of phenanthroine-Fe2+ oxidation method,DPPH and auto-oxidation of pyrogallol were used to measure the effects of different concen-trations of SPK on scavenging hydroxyl (OH-) free radical,DPPH free radical and superoxide anion (O2-) free radical;IC50 of SPK was calculated. Prussian blue reaction was used to determine total reducing ability(by absorbance)of different concentrations of SPK to Fe3+. Vitamin C(VC)was used as positive control in above trials. RESULTS:SPK could eliminate the OH-free radical, DPPH free radical and O2- free radical in concentration-dependant manner,and the maximum elimination rate of SPK to OH- free radical and DPPH free radical were 86.82% and 78.98%(IC50 were 54.31,0.636 g/L),which were higher than VC (64.77%, 73.49%). The maximum elimination rate of SPK to O2- free radical was 78.31%(IC50 was 3.918 g/L),which was lower than VC (94.14%). In reducing ability test,SPK improved absorbance in reducing ability test system,and maximum absorbance was simi-lar to VC in concentration-dependant manner. CONCLUSIONS:SPK has obvious anti-oxidant activities in vitro.

BACKGROUND: Classic isolation method of bone marrow mesenchymal stem cells (BMSCs) is Percoll density gradient centrifugation. Blood cell component was removed. However, this method is complicated. Preparation density was needed when isolating dog bone marrow. Moreover, centrifugation was frequent, which had a great damage to cells. OBJECTIVE: To establish methods of the isolation, proliferation, culture and osteoinduction of canine BMSCs, and observe the in vitro proliferation and ability to osteoinduction differentiation. METHODS: 10 mL bone marrow was extracted from dog posterior superior iliac spine, heparin anticoagulation, diluted using Hanks juice, treated with 1.077 g/mL Ficoll solution 3 mL, and centrifuged at 2 000 r/min for 20 minutes. Karyocytes were absorbed to form white cloudlike layering interface, and then centrifuged twice using DMEM supplemented with fetal bovine serum, incubated at 12×10~4/cm~2 at 37 ℃ in a 5% CO_2 incubator. Following subculture, cells were incubated in DMEM containing dexamethasone, β-sodium phosphoglycerol and ascorbic acid 2-phosphate. Immunocytochemical staining and immunofluorescence staining were utilized to detect osteocalcin, osteopontin and type Ⅰ collagen expression in osteoblasts. Alkaline phosphatase staining and alizarin red staining were performed. RESULTS AND CONCLUSION: 1.077 g/mL Ficoll density gradient centrifugation was used to isolate karyocytes that were significant compared with Percoll solution. Obtained BMSCs had high purity, good growth and the mean doubling time was 24 hours. Following in vitro osteogenic incubation of dog BMSCs, osteocalcin, osteopontin and type Ⅰ collagen showed positive expression. Alkaline phosphatase staining demonstrated bluish-green cytoplasm. Alizarin red staining showed red nodes in extracellular matrix, and could differentiate into osteoblasts in vitro.