Objective To observe the effect of puerarin injection (PI) on insulin resistance in gestational diabetic mellitus(GDM) rats. Methods Rats with gestation for 5 days received onedose injection of STZ 35 mg/kg into subaortic caudal vein. After injection for 72 hours, rats with blood sugar over 13.5 mmol/L indicated the modeling being successful. Then the model rats were divided into treatment group (TG, using Puerarin), westernmedicine control group(WCG, using rosiglitazone) and model group (MG, using 0.9 % NaCl), 10 rats in each group .Ten normal gestational rats were used as normal control group (NCG, using 0.9 % NaCl).Two weeks later, the changes of blood sugar, Creactive protein (CRP) and tumor necrosis factor- ? (TNF- ? ) were observed. Results Compared with MG, the above indexes were improved in TG and WCG after treatment( P

Objective To evaluate the effectiveness of aerobic exercise on quality of life in breast cancer survivors.Methods Electronic databases were searched.Randomized controlled trials in which aerobic exercise was compared with controls regarding quality of life in breast cancer survivors were included.Results We analyzed 12 RCT studies.Aerobic exercise significantly improved quality of life in breast cancer survivors.Seven studies which used FACT-B scale concluded that aerobic exercise could significantly improve psychological emotion,social/family function and breast-related function.The aerobic exercise aiming at breast cancer survivors who had already finished all the therapies except endocrine therapy of breast cancer,mainly comprised of short-period intervention programs (within 24 weeks),and was advised to be executed under professionals' supervision to ensure safety.Conclusion Aerobic exercise could effectively improve quality of life in breast cancer survivors,especially in aspects of psychological emotion,social/family function and breast-related function.

Objective To study the effect of anti-hepatitis B virus of Lamivudine combined with Saikosaponins in vivo. Methods Chongqing duck hepatitis B model was selected and given to Lamivudine combined with Saikosaponins(combined group) oral treatment 28d, then the ducks were observed 7d after drug withdrawal. The levels of serum HBV DNA, HBsAg and transaminase(ALT, AST) were detected before and after treatment, and compared with that in Lamivudine group and Saikosaponins group as control. Results In combined group,the levels of serum HBV DNA and HbsAg were significantly decreased(P ＜ 0.05), and 7d after drug withdrawal the levels of serum HBV DNA and HBsAg showed no" anti-jump" compared with that at drug treatment 28d(P ＞ 0.05). In Lamivudine group and Saikosaponins group the levels of those increased again 7d after drug withdrawal (P ＜ 0. 05). In each group the levels of serum ALT and AST did not change within the same phase during drug treatment 28d and 7d after drug withdrawal(P ＞ 0. 05). Conclusion Lamivudine combined with Saikosaponins in vivo had the effect of anti-hepatitis Bvirus, and the effect was stable and lasting.

Objective To explore the manufacture methods of a new nasal ice compress cover,and to observe its clinical application effect.Methods The nasal ice compress cover body is a silica gel cover,which contains the upper cover body and the lower cover body.The shape of the upper cover body is consistent to the shape of frontal temporal while the shape of the lower cover body is consistent to the shape of the nose root,and the upper cover body and the lower cover body are integrally formed.The inner surface of the cover body is equipped with a ice pack,and the ice pack is of the same shape with the inner surface.And the cover body is provided with two elastic bands.Patients of the experimental group use the nasal ice compress cover and patients of the control group use the traditional ice hockey ice so as to detect the clinical effect of the new nasal ice compress cover and satisfaction rate and adverse reaction of patients.Results The new nasal ice compress cover has good clinical application effect,and patients consider that it has the advantages of convenient,effective and highly satisfactory. Conclusion The new nasal ice compress cover improves patients’comfort degree and satisfaction rate,and it is worthy of wide promotion.

Objective To optimize the best techniques of preparing baicalin gelatin microspheres with orthogonal experimental design.Methods Baicalin gelatin microspheres were prepared by emulsion chemical-cross linking method with degradable gelatin as carrier,liquid paraffin as oil phase,Span-80 as emulsifier.The particle size,containing efficiency of microspheres were determined and technique reproducibility was studied.Results Microspheres with good shape,smooth surface and narrow size distribution were prepared.The average diameter of microspheres was 27.47 ?m,the loading amount of medicine was 8.18% and encapsulation efficiency was 70.21%.Conclusion The preparation procedure established was stable and practical.

0.05),(5.45 ? 0.58)% and (11.60 ? 0.87)%,(P 0.05)of the control team and test team. Conclusions 125Ⅰ seeds implantation can induce tumor cell apoptosis, beginning at 72 h and reached peak at 2 w and kept the high level here afterword. The apoptosis rate descended rapidly along with the increase of distance away from the 125Ⅰ seedling.

Objective To explore the classical clinical features of mitochondrial encephalomyopathy with lactic acidosis and stroke like episodes (MELAS) syndrome.Methods The clinical data of 1 MELAS syndrome patient was analyzed retrospectively.Results This patient suffered from the symptoms of stroke such as speech disorder and abnormal behavior at the beginning.She had a medical history of maternal family.There were headache and seizure in the process of the disease.The result after a physical examination showed that the patient had hearing loss, visual field defect and decrease of muscle strength.The CT and MRI scan of head showed that the patient had a stroke like lesion which did not follow the distribution of blood vessels.The blood and cerebrospinal fluid examination has ruled out viral and autoimmune encephalitis.The final diagnosis of MELAS syndrome was confirmed by gene analysis.The gene mutation was the m.3243A>G mutation.The clinical symptoms of the patient were comprehensive and the imaging findings were typical.Conclusions Main clinical manifestations of MELAS syndrome are stroke like seizures, epilepsy, headache, dementia, hearing impairment, peripheral neuropathy, myopathy, lactic acidosis, diabetes and so on.Main imaging features of the disease are stroke like lesions, basal ganglia calcification and brain atrophy.

Objective:To investigate the role of apoptosis-associated speck-like protein(ASC) in non-apoptotic,non-necrotic cell death of neutrophils induced by ONO-AE-248.Methods:The morphological changes of neutrophil nuclei and DNA fragmentation of the cells were examined by confocal microscopy and terminal-deoxynucleotidyl transferase mediated nick end labeling(TUNEL).Neutrophils were stimulated with medium alone,LPS,TNF-? or ONO-AE248 respectively.Then expression of ASC was detected by Western blot.Results:ONO-AE-248-induced neutrophil death was negative in TUNEL assay.Western blot showed that the expression of ASC protein was down-regulated by ONO-AE-248.Conclusion:The DNA degradation of the neutrophils when stimulated by ONO-AE-248 is probably different from that of the typical apoptosis.ASC might be a crosslink point between the ONO-AE-248-induced neutrophil death and spontaneous apoptosis of neutrophils.

Objective To explore the association between the minimum temperature in different seasons and the hospital emergency room visits for cardiocerebrovascular diseases(International Classification of Diseases,tenth vision ICD-10:I00-I99) in Beijing,China.Methods The data of the daily hospital emergency room visits for cardiocerebrovascular diseases(ICD-10:I00-I99)from one of Peking university hospitals,the data of relevant ambient air pollution from the Beijing Municipal Environmental Monitoring Center and the data of meteorological index from China meteorological data sharing service system between 2004— 2006 were collected The time-stratified case-crossover design was used to analyze the data.Results After adjusting SO2,NO2,and PM10,the results showed that each 1 ℃ increase in the daily minimum temperature in summer was associated with the intraday hospital emergency room visits(OR=1.039,P

Objective To obtain anamorphosis of Shiraia bambusicola producing the component,hypocrellin A effectively.Methods A strain was isolated from the fruit of S.bambusicola by separating its hypha and was cultured by solid-state fermentation.One of the pure chemical compounds isolated from the stroma of wild S.bambusicola was studied on the basis of UV/VIS,IR,MS spectrometric analysis and physicochemical constants.Results The pigment is proved to be hypocrellin A.The isolate was determined as the stable anamorphosis strain 0258 of S.bambusicola.Conclusion A namorphosis strain of S.bambusicola is obtained and precisely appraised for the first time.This study will provide basis for the future massively industrialized hypocrellin production.