This study was aimed to establish the TLC identification method of Radix Mirab ilis himalaic a. The β-sitosterol and daucosterol were used as the reference substances. The single-factor test was used. A variety of factors which affected TLC were systematically investigated to filter out the best TLC conditions for identification of different batches of medicines. The results showed that the best TLC conditions were as follows: silica gel G plates, extraction solvent (methanol), reagent (5% sulfuric acid in ethanol), extraction method (ultrasonic extraction with methanol), ex-tracted time (30 min), the agent (petroleum ether-ethyl acetate-acetone (5:2:1)) and sample volume (6 μL). It was concluded that the method, which had high separation degree, was reproducible and simple. It can be used as the quality control of Radix Mirab ilis himalaic a.

Objective To compare differences and similarities of the content of trigonelline in Himalaica mirabilis of either wild or cultivated materials from different places. Methods An HPLC method was established to determine the content of trigonelline in Himalaica mirabilis. ZORBAX XDB-CN column (4.6 mm×250 mm, 5 μm) was used, with mobile phase of acetonitrile-0.03%acetic acid (85∶15), flow rate of 0.8 mL/min, detection wavelength of 265 nm, determination wavelength of 360 nm, and column temperature of 30 ℃. Results Regression calculation was made on peak area with the reference solution concentration, and then got the regression equation A=23.409C-26.398, r=0.999 8. Trigonelline showed good linear relation with peak area among the range of 2.004-200.400 μg/mL. The average recovery of trigonelline was 99.57%, RSD=1.11%. Conclusion There was no significant difference in the content of trigonelline of either wild or cultivated materials from different places. This study laid the foundation of application of the cultivated Himalaica mirabilis.

Objective Fructus Amomi(Sharen) is derived from the dry ripe fruit of Amomum villosum Lour., A.villosum Lour. var. xanthioides T.L. Wu et Senjen and A.longiligulate T.L.Wu, which is widely utilized for its clinic effects on digestive system. However, Fructus Amomi from different species and habitats, possessing different quality, is difficult to identify. In this study, we aim to develop a simple, rapid and reliable method for authentication of Fructus Amomi. Methods Twenty-five batches of samples of Fructus Amomi were collected and electronic nose was introduced into analyzing their odor with multiple mathematical statistics methods. Na?ve bayes network (NBN), radical basis function (RBF) and random forest (RF) were applied to establish different classifiers while BestFirst+CfsSubsetEval (BC) was used to screen the attributes for searching sensor array with higher contributions. Results Firstly, after attribute-screening via BC, the established discriminative models via NBN, RBF and RF could successfully identify genuine and non-genuine samples, presenting correct judging ratios of 78% and 84% through ten-fold cross validation and external test set validation, respectively. Besides, quantity predictive models were constructed as well. In case of content of bornyl acetate, one of the effective components in Fructus Amomi, values were higher than 3.5 mg/g and lower than 1.8 mg/g with sensor response of 0.04 and 0.03, respectively. Conclusion In this paper, quality discriminative model and quantity predictive model of Fructus Amomi were established via electronic nose and multiple mathematical statistics methods. It indicates that electronic nose could be a promising method for quality evaluation of Chinese material medica.

This study was aimed to identify Chrysanthemi Flosbefore and after sulfur fumigation based on its different odour by the electronic nose technology.It was expected to explore a new method for the Chrysanthemi Flos identification according to the odour.The electronic nose technology was used in the detection of peak response values of Chrysanthemi Flos on sensor array.The principal component analysis (PCA) and 10 machine learning (ML) ways were used in the analysis of response values and establishment of optimized identification models.The results showed that there was a significant difference in the odour between sulfur fumigated Chrysanthemi Flos and non-sulfur fumigated ones.The identification models were successful with high correct judge rate by PCA and 6 ML ways including BF Tree,J48 and Random Tree.It was concluded that the electronic nose technology can be used for the accurate identification of sulfur fumigated Chrysanthemi Flos and non-sulfur fumigated ones.The electronic nose technology combined with multiple ML methods can be introduced in the quality evaluation of Chrysanthemi Flos.It provided more ideas for the application of electronic nose in data mining for traditional Chinese medicine (TCM) studies.

AIM: A new HPLC-MS method was developed to determine finasteride in human plasma. METHODS: Two formulations of finasteride tablets were given to 20 healthy male volunteers according to a randomized 2-way cross-over design. The samples were extracted by ethyl acetate under basic conditions, then were separated by C18 column and determined by mass detector. RESULTS: The calibration curve of finasteride was linear and intra-day and inter-day RSD were less than 10 %. The pharmacokinetics parameters of the two formulations (4.5 ± 0.5) h for t1/2; (3.0 ± 0.7) and (2.8 ± 0.9) h for tmax, respectively. The results indicated that there was no significant difference on cmax, A UC0-24, t1/2 or tmax values between the two formulations. CONCLUTION: The relative bioavailability of tablets I with respect to tablets Ⅱ is (99.3 ± 9.2) % by the A UC0-24 measurement, and bioe quivalence is observed between the two tablets.

As a progressive and chronic intractable disease, chronic pancreatitis (CP) is generally manifested as early chronic pain, and then exocrine and endocrine insufficiency, and various complications in clinical course. The complex clinical manifestations lead to the controversies over treatment strategies at present, involving a multidisciplinary treatment (MDT) approach. It can enable different professional medical experts to discuss the diagnosis and treatment for patients together during a specific period (online or offline), which is an effective mode for diagnosing and treating complex diseases nowadays. MDT for CP usually begins with lifestyle intervention and drug therapy, and then goes with endoscopic interventions and surgical resection, or their combination. This article reviewed the current status on MDT approaches for CP and shared the MDT experience from Changhai Hospital in order to improve the management of CP course.

Objective:To analyze the research hotspots and evolution trends of diabetes patients with sarcopenia in the past decade, and to provide ideas for domestic research on diabetics patients with sarcopenia.Methods:Relevant literature on diabetics patients with sarcopenia included in the Web of Science from 2011 to 2021 was retrieved. CiteSpace software was used to analyze country, cited journals, cited literature and high-frequency keywords.Results:A total of 606 papers were included, and the number of papers published increased in the past decade. The country with the most publications was Japan. Research hotspots included the use of diabetic sarcopenia assessment tools, pathogenesis, and the prediction of other disease-related factors. However the number of intervention research was small.Conclusions:In clinical work, health care professionals and related workers should pay attention to the importance of disease care and self-management of diabetic patients, provide personalized care and treatment to reduce or prevent the occurrence of sarcopenia, so as to promote the research of diabetic sarcopenia in China.

Objective To analyze the publication profile and research hotspots of studies at home and abroad on postoperative cognitive dysfunction by bibliometric methods.Methods Litera-tures related to postoperative cognitive dysfunction published in the China National Knowledge Infra-structure(CNKI)and Web of Science databases from 2013 to 2023 were included,and statistical a-nalysis was conducted by CiteSpace software.Results A total of 2 705 Chinese literatures and 2 412 English core literatures were included.The number of literatures on postoperative cognitive dysfunc-tion showed an annual increase from 2013 to 2023;China was the country with the highest number of publications internationally,with Capital Medical University ranking the top among Chinese institu-tions;domestically,postoperative cognitive dysfunction researches predominantly focused on clinical studies,while overseas researches emphasized pathological mechanisms and basic investigations.Re-search hotspot analysis revealed an enhanced interest in the inflammatory mechanisms and anesthetic as-pects of postoperative cognitive dysfunction.Conclusion From 2013 to 2023,the overall research at home and abroad interest in postoperative cognitive dysfunction has shown an upward trend,yet its patho-physiological mechanisms need further exploration.The risk factors for postoperative cognitive impairment and their correlations with anesthesia and mortality are being actively explored,and new methods for prevention and treatment such as percutaneous electrical stimulation are gradually emerging.

Objective To analyze the publication profile and research hotspots of studies at home and abroad on postoperative cognitive dysfunction by bibliometric methods.Methods Litera-tures related to postoperative cognitive dysfunction published in the China National Knowledge Infra-structure(CNKI)and Web of Science databases from 2013 to 2023 were included,and statistical a-nalysis was conducted by CiteSpace software.Results A total of 2 705 Chinese literatures and 2 412 English core literatures were included.The number of literatures on postoperative cognitive dysfunc-tion showed an annual increase from 2013 to 2023;China was the country with the highest number of publications internationally,with Capital Medical University ranking the top among Chinese institu-tions;domestically,postoperative cognitive dysfunction researches predominantly focused on clinical studies,while overseas researches emphasized pathological mechanisms and basic investigations.Re-search hotspot analysis revealed an enhanced interest in the inflammatory mechanisms and anesthetic as-pects of postoperative cognitive dysfunction.Conclusion From 2013 to 2023,the overall research at home and abroad interest in postoperative cognitive dysfunction has shown an upward trend,yet its patho-physiological mechanisms need further exploration.The risk factors for postoperative cognitive impairment and their correlations with anesthesia and mortality are being actively explored,and new methods for prevention and treatment such as percutaneous electrical stimulation are gradually emerging.

Objective:To conduct the visual analysis of emergency palliative care based on the Web of Science database.Methods:The literature on emergency palliative care collected from 2012 to 2022 was systematically searched in the Web of Science core collection. CiteSpace software was used for visual analysis.Results:A total of 630 articles were included, and the number of publications was on the rise. The country with the highest number of publications was the United States, with 8 out of the top 10 institutions in terms of publication volume coming from the United States. The author with the highest number of publications was Grudzen. The top 10 journals in terms of citation frequency were mostly specialized journals on palliative care and first aid. The results of keyword analysis showed that a total of 15 clustering labels and 20 emergent words were output, reflecting the changes of key research objects, main research types and areas of concern.Conclusions:At present, nursing research in this field is in the development stage in China. We can draw on the practical experience of foreign countries to further explore the emergency palliative care model and service system based on China's national conditions.