1.Influence of Zidian Granule on the Peripheral Blood Platelet in Mouse Model of Idiopathic Thrombocytopenic Purpura
Ying FAN ; Ji MA ; Shuchun TAO ; Jiahui WANG ; Ying REN
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(03):-
Objective To investigate the changes of the peripheral blood platelet counting and function the mouse model of idiopathic thrombocytopenic purpura (ITP) and to observe the effect of Zidian Granule (ZG). Methods The peripheral platelet counting was conducted with the automatic hemocyte analyzer; CD41 andCD61 level was measured with flow cytometry. Results The amount of the peripheral blood platelet in ITP model mouse was decreased after injection of antiplatelet serum (APS) and CD41 and CD61 were also decreased.(P
2.Methodological Research on TLC Identification of Radix Mirabilis himalaica
Hui LIN ; Ting ZHAO ; Qingjia REN ; Huiqin ZOU ; Jiahui LI ; Lian PENG ; Zhiyu REN ; Yonghong YAN
World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(12):2725-2730
This study was aimed to establish the TLC identification method of Radix Mirab ilis himalaic a. The β-sitosterol and daucosterol were used as the reference substances. The single-factor test was used. A variety of factors which affected TLC were systematically investigated to filter out the best TLC conditions for identification of different batches of medicines. The results showed that the best TLC conditions were as follows: silica gel G plates, extraction solvent (methanol), reagent (5% sulfuric acid in ethanol), extraction method (ultrasonic extraction with methanol), ex-tracted time (30 min), the agent (petroleum ether-ethyl acetate-acetone (5:2:1)) and sample volume (6 μL). It was concluded that the method, which had high separation degree, was reproducible and simple. It can be used as the quality control of Radix Mirab ilis himalaic a.
3.Content Determination of Trigonelline in Wild and Cultivated Himalaica Mirabilis of Different Districts
Hui LIN ; Ting ZHAO ; Qingjia REN ; Huiqin ZOU ; Zhiyu REN ; Jiahui LI ; Lian PENG ; Yonghong YAN
Chinese Journal of Information on Traditional Chinese Medicine 2014;(12):76-78,79
Objective To compare differences and similarities of the content of trigonelline in Himalaica mirabilis of either wild or cultivated materials from different places. Methods An HPLC method was established to determine the content of trigonelline in Himalaica mirabilis. ZORBAX XDB-CN column (4.6 mm×250 mm, 5 μm) was used, with mobile phase of acetonitrile-0.03%acetic acid (85∶15), flow rate of 0.8 mL/min, detection wavelength of 265 nm, determination wavelength of 360 nm, and column temperature of 30 ℃. Results Regression calculation was made on peak area with the reference solution concentration, and then got the regression equation A=23.409C-26.398, r=0.999 8. Trigonelline showed good linear relation with peak area among the range of 2.004-200.400 μg/mL. The average recovery of trigonelline was 99.57%, RSD=1.11%. Conclusion There was no significant difference in the content of trigonelline of either wild or cultivated materials from different places. This study laid the foundation of application of the cultivated Himalaica mirabilis.
4.Role of NO signal in ABA-induced phenolic acids accumulation in Salvia miltiorrhiza hairy roots.
Lihong SHEN ; Jiahui REN ; Wenfang JIN ; Ruijie WANG ; Chunhong NI ; Mengjiao TONG ; Zongsuo LIANG ; Dongfeng YANG
Chinese Journal of Biotechnology 2016;32(2):222-230
To investigate roles of nitric oxide (NO) signal in accumulations of phenolic acids in abscisic.acid (ABA)-induced Salvia miltiorrhiza hairy roots, S. miltiorrhiza hairy roots were treated with different concentrations of sodium nitroprusside (SNP)-an exogenous NO donor, for 6 days, and contents of phenolic acids in the hairy roots are determined. Then with treatment of ABA and NO scavenger (2-(4-carboxy-2-phenyl)-4,4,5,5-tetramethylimidazoline-1- oxyl-3-oxide, c-PTIO) or NO synthase inhibitor (NG-nitro-L-arginine methyl ester, L-NAME), contents of phenolic acids and expression levels of three key genes involved in phenolic acids biosynthesis were detected. Phenolic acids production in S. miltiorrhiza hairy roots was most significantly improved by 100 µmoL/L SNP. Contents of RA and salvianolic acid B increased by 3 and 4 folds. ABA significantly improved transcript levels of PAL (phenylalanine ammonia lyase), TAT (tyrosine aminotransferase) and RAS (rosmarinic acid synthase), and increased phenolic acids accumulations. However, with treatments of ABA+c-PTIO or ABA+L-NAME, accumulations of phenolic acids and expression levels of the three key genes were significantly inhibited. Both NO and ABA can increase accumulations of phenolic acids in S. miltiorrhiza hairy roots. NO signal probably mediates the ABA-induced phenolic acids production.
Abscisic Acid
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pharmacology
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Benzofurans
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metabolism
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Free Radical Scavengers
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pharmacology
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Hydroxybenzoates
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metabolism
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Nitric Oxide
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metabolism
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Phenylalanine Ammonia-Lyase
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metabolism
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Plant Roots
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metabolism
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Salvia miltiorrhiza
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metabolism
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Tyrosine Transaminase
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metabolism
5.Quality and quantity classification models of Fructus Amomi applying electronic nose with multiple mathematical statistics methods
Huiqin ZOU ; Jianting GONG ; Liying ZHAO ; Ou TAO ; Jiahui LI ; Zhiyu REN ; Yonghong YAN
Journal of International Pharmaceutical Research 2015;(4):513-518
Objective Fructus Amomi(Sharen) is derived from the dry ripe fruit of Amomum villosum Lour., A.villosum Lour. var. xanthioides T.L. Wu et Senjen and A.longiligulate T.L.Wu, which is widely utilized for its clinic effects on digestive system. However, Fructus Amomi from different species and habitats, possessing different quality, is difficult to identify. In this study, we aim to develop a simple, rapid and reliable method for authentication of Fructus Amomi. Methods Twenty-five batches of samples of Fructus Amomi were collected and electronic nose was introduced into analyzing their odor with multiple mathematical statistics methods. Na?ve bayes network (NBN), radical basis function (RBF) and random forest (RF) were applied to establish different classifiers while BestFirst+CfsSubsetEval (BC) was used to screen the attributes for searching sensor array with higher contributions. Results Firstly, after attribute-screening via BC, the established discriminative models via NBN, RBF and RF could successfully identify genuine and non-genuine samples, presenting correct judging ratios of 78% and 84% through ten-fold cross validation and external test set validation, respectively. Besides, quantity predictive models were constructed as well. In case of content of bornyl acetate, one of the effective components in Fructus Amomi, values were higher than 3.5 mg/g and lower than 1.8 mg/g with sensor response of 0.04 and 0.03, respectively. Conclusion In this paper, quality discriminative model and quantity predictive model of Fructus Amomi were established via electronic nose and multiple mathematical statistics methods. It indicates that electronic nose could be a promising method for quality evaluation of Chinese material medica.
6.Identification of Sulfur Fumigated Chrysanthemi Flos with Electronic Nose Technology
Zhiyu REN ; Jianting GONG ; Liying ZHAO ; Huiqin ZOU ; Jiahui LI ; Yonghong YAN
World Science and Technology-Modernization of Traditional Chinese Medicine 2015;(11):2405-2409
This study was aimed to identify Chrysanthemi Flosbefore and after sulfur fumigation based on its different odour by the electronic nose technology.It was expected to explore a new method for the Chrysanthemi Flos identification according to the odour.The electronic nose technology was used in the detection of peak response values of Chrysanthemi Flos on sensor array.The principal component analysis (PCA) and 10 machine learning (ML) ways were used in the analysis of response values and establishment of optimized identification models.The results showed that there was a significant difference in the odour between sulfur fumigated Chrysanthemi Flos and non-sulfur fumigated ones.The identification models were successful with high correct judge rate by PCA and 6 ML ways including BF Tree,J48 and Random Tree.It was concluded that the electronic nose technology can be used for the accurate identification of sulfur fumigated Chrysanthemi Flos and non-sulfur fumigated ones.The electronic nose technology combined with multiple ML methods can be introduced in the quality evaluation of Chrysanthemi Flos.It provided more ideas for the application of electronic nose in data mining for traditional Chinese medicine (TCM) studies.
7.Clinical analysis of type A aortic dissection with acute lower limb ischemia
Wei REN ; Zhiwei WANG ; Zhiyong WU ; Jinxing CHANG ; Yongle RUAN ; Jiahui WANG
Chinese Journal of Thoracic and Cardiovascular Surgery 2019;35(3):154-158
Objective To summarize the clinical experience and efficacy of surgical treatment for Stanford type A aortic dissection leading to acute lower limb ischemia.Methods From January 2014 to January 2018,12 patients with severe lower limb ischemia caused by acute type A aortic dissection were treated with Suns surgery.Among them,11 patients were treated with restoration of lower limb blood supply preferentially,including 10 cases of femoral artery bypass and 1 case of abdominal aorta-iliac artery stent graft implantation.Another case was treated with ascending aorta-femoral artery bypass after Sung surgery.Results 3 cases died of ischemia and necrosis of the lower extremities.Two of them died of multiple organ failure due to amputation and one died of low cardiac output due to refractory acidosis.Acute renal failure performed bedside CRRT in 5 patients and ECMO in 1 patient.The remaining 9 patients were discharged from the hospital and the symptoms of lower limb ischemia disappeared.After an average follow-up of 23 months,the re-examination of the aorta CTA showed that the bypass artery was unobstructed and the distal femoral artery was well developed.One patient infecting vascular prosthesis was cured by taking out the unit.Conclusion For acute lower limb ischemia caused by type A aortic dissection,blood flow of lower extremities should be restored as soon as possible to reduce mortality and complications.Femoral artery bypass and abdominal aorta-iliac arterial repair are simple and effective in reconstructing lower limb blood supply.
8.Study on the mechanism of Danpi-Chishao in the treatment of sepsis based on network pharmacology
Jiahui SU ; Caijun WU ; Fuyao NAN ; Huan XIA ; Yang REN ; Linqin MA
Journal of Chinese Physician 2023;25(2):178-185
Objective:To analyze the mechanism of Danpi-Chishao in treatment of sepsis based on network pharmacology.Methods:The corresponding targets of Danpi-Chishao and sepsis were carried out through TCMSP database, OMIM database and Genecards database. Cystoscope 3.8.2 software was used to construct the " Chinese medicine-active components-target-disease" network diagram. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were carried out by DAVID database. Weisheng cloud platform was used to draw bubble map.Results:A total of 36 effective components of Danpi-Chishao was obtained, mainly including quercetin, kaempferol, baicalin, β-sitosterol, stigmasterol, paeoniflorin and so on. There were 96 potential common key targets between Danpi-Chishao and sepsis, such as prostaglandin-endoperoxide synthase 2 (PTGS2), transcription factor p65 (RELA), phosphatidylinositol-4, 5-bisphosphate 3-kinase catalytic subunit gamma (PIK3CG), B-cell lymphoma 2 (BCL-2)-associated X (BAX), BCL-2, Caspase-3 (CASP3) with a degree value>4.9. The result of protein-protein interaction (PPI) network analysis showed that there were 10 important target proteins, including alpha serine/threonine-protein kinase (AKT1), interleukin-6 (IL-6), tumor necrosis factor (TNF), interleukin-1β (IL-1β), vascular endothelial growth factor A (VEGFA), cellular tumor antigen p53 (TP53), matrix metalloproteinase-9 (MMP9), CASP3, PTGS2, C-C motif chemokine ligand 2 (CCL2). The pathways obtained by GO and KEGG enrichment analysis included atherosclerosis pathway, advanced glycation end products (AGE)-receptor for advanced glycation end products (RAGE) signal pathway, cancer pathway, tumor necrosis factor signal pathway, hypoxia-inducible factor (HIF) signal pathway, IL-17 signal pathway and other pathway.Conclusions:The mechanism of the intervention effect of Danpi-Chishao on sepsis may be that the active components such as quercetin, kaempferol, paeoniflorin act on target proteins such as PTGS2, RELA, PIK3CG, BAX, BCL2, CASP3, and through TNF-related signal pathway, HIF-1 signal pathway, IL-17 signal pathway, etc. Nonetheless, the conclusion needs further experimental verification.
9.Prevalence of loss of activities of daily living and influencing factors in elderly population in China
Jiahui QIAN ; Kan WU ; Huiqiang LUO ; Peiya CAO ; Xiaohui REN
Chinese Journal of Epidemiology 2016;37(9):1272-1276
Objective To investigate the prevalence of the loss of basic activities of daily living (ADL) and instrumental ADL (IADL) influencing factors in the elderly population in China and provide evidence for the effective keeping and improvement of the elderly daily living.Methods The information about demographic characteristics and activities of daily living of elderly Chinese aged ≥ 60 years were collected by using the data of 2013 China Health and Retirement Longitudinal Study.The elderly's ADL of taking bath,eating,getting in and out of bed,dressing,toilet use,and defecating and the IADL of doing housework,cooking,making phone call,taking medicine,shopping and money managing were evaluated.The differences in loss of ADL among different populations were compared by x2 test and logistic regression analysis was conducted to identify influencing factors for the loss of ADL and IADL.Results The overall ADL loss rate was 23.8% and the overall IADL loss rate was 35.4% in elderly Chinese.The proportion of having trouble in toilet use was highest among all ADL items,followed by bathing and getting in and out of bed.The proportion of having trouble in making phone call was highest among all IADL items,followed by doing housework and money managing.Female,older age,low educational level,living in central and western China,chronic diseases and disability were factors associated with ADL loss and IADL loss;the divorced or separated,widowed were more likely to have ADL loss;people living in rural area were more likely to have IADL loss.Conclusion It is necessary to take comprehensive measures to delay and reduce the ADL and IADL loss in elderly Chinese and provide timely and appropriate health care for the elderly with ADL or IADL loss.
10.Effects of Different Processing Methods on the Volatile Components of Amomi Fructus Based on GC-MS and Multivariate Statistical Analysis
Jiaxin LI ; Lingjuan ZHOU ; Lixia ZHANG ; Jing SU ; Jiahui REN ; Tianhao ZHOU ; Jinhui WANG ; Guang LI
Herald of Medicine 2024;43(4):607-613
Objective To analyze the influence of different processing methods,including frying,ginger frying,and salt frying,on the volatile components of A.fructus.Methods The volatile components in different processed products of A.fructus were detected and analyzed by gas chromatography-mass spectrometry(GC-MS)based on multivariate statistical analysis.After OPLS-DA analysis,the different components were screened under the conditions of VIP>1.5 and P<0.05 and were qualitatively searched using the NIST 11 spectral library.Results A total of 49 different components were identified,with 14 components only changing in the seed mass and 22 components changing in the peel.The content of camphor could be significantly reduced in the seed mass after A.fructus was processed and the content of bornyl acetate significantly increased in the peel of frying A.fructus.Salt frying had a great influence on the alkanes in A.fructus,and ginger processing did not only increase the volatile components in ginger,which reflected the complexity of the processing mechanism.Conclusion At present,the specific processing mechanism is not clear,but the experimental results provide theoretical data for the "detoxification and efficiency enhancement" effect of A.fructus processing,reflecting the scientific nature of the processing,enriching the processing theory of A.fructus,and providing a reference for further in-depth research on the activity of different processed products of A.fructus.