Cerebral palsy (CP) is a group of syndrome caused by a non-progressive brain injury that occurs from before birth to 28 days after birth. The principal symptoms of CP are abnormal centrocinesia and irregular posture, or accompanied by intelligence disorders, seizures, dystropy, sensory impairments and other dysfunctions. To evaluate the function of children with cerebral palsy can assess disfuction level,judge clinical classification, estimate prognosis, and more importantly guide to establish treatment plan and evaluate therapeutic efficacy. At present, there are no unified standards to evaluate CP at home and abroad, So this article will overview evaluation methods of CP commonly used in recent years.

Objective To compare the clinical effect and adverse reactions of different concentrations of dezocine epidural anesthesia for cesarean section.Methods According to digital table,120 cases of elective cesarean section were randomly divided into 4 groups with 30 cases in each group, the group D1 was given with dezocine 2.5mg,the group D2 with dezocine 5mg,the group D3 with dezocine 10mg,the group M with morphine 2mg.All the drugs used in postoperative epidural analgesia after cesarean section.To observe resting pain and conduct the VAS scoring at postoperative 4,8,12,24h and record various adveme reactions.Results The VAS scores at postoperative 4,8,12h in the group D1 were lower than those in the D2,D3,M with statistical difference(all P<0.05).The VAS scores at postoperative 4,24h had no statistical difference among D2,D3,M groups(all P>0.05).The VAS scores at postoperative 8h in the group D3 were lower than those in the D2,D3,M with statistical difference(all P<0.05). There were no statistical differences among 4 groups at postoperative 24h(all P>0.05).The incidence rates of nause-a and vomiting the group M were higher than that in the group D1,D2 and D3,showing statistical difference among them(P<0.05).Conclusion Appropriate concentrations of dezocine used in postoperative epidural analgesia after cesarean section has definite effect and less adverse reactions.

Objective To observe the influence of the insulation blanket applied in general anesthesia on the incidence of postoperative delirium.Methods 60 patients with elective laparoscopic cholecystectomy under generalanesthesia with ASA of grade Ⅰ-Ⅱ were selected.According to the random number table,the patients were divided into group A(without insulation blanket)and group B (with insulation blanket),each group in 30cases.The dosage and drug batch number of anesthetic drugs were same.Evaluated each patient using the confusion assessment method-chinese revised (CAM-CR).Recorded the number of cases of delirium for each group when 24 hours,48 hours, 72 hours after operation.Results The incidence of postoperative delirium in group A was 6.7%,which in group B was 0.0%.The incidence of postoperative delirium of group B was lower than group A (P <0.05 ).Conclusion Using insulation blanket to protect body temperature in perioperative operation can reduce the incidence of postopera-tive delirium in perioperative operation.

Objective We have examined tumor suppressor gene p53 from N- methyl - N- nitrosourea (MNU ) induced the col - orectal tumors in rats. Methods PCR, SSCP and DNA sequence analysis. Results In colorectal tumors induced by MNU, the mobil- ity shifts of p53 gene mutation were observed, p53 gene mutation was a deletion at third position C of codon 244 in exon7. Conclu- sion The present study suggested that MNU induces rat p53 mutations in the colorectal tumors.

Objective To explore the protective effect of bone marrow mesenchymal stem cells (MSC) on diabetic myocardium and anoxic pre-conditioning (AP).Methods Eight-week-- old male Sprague-Dawley rats were given with a single intraperitoneal injection of streptozotocin (STZ) (60 mg/kg)to induce diabetes mellitus (DM).Donor rats were 8-week-old male Sprague - Dawley rats.Before transplantation,MSC were incubated in CM-DiI at a concentration of 2 μg/mL for 20 min.AP-MSC were exposed to 3 hours of anoxia.At 4 months after STZ injection,diabetic rats were randomly given with an intramyocardial injection of one of the followings:150 μL of Dulbecco's modified Eagle's medium ( DMEM),5 ×106 MSC/150 μL,or 5 × 106 AP - MSC/150 μL (n =10 for each group).Three months after STZ injection and 2 weeks after transplantation,we evaluated the cardiac function by echocardiography,and also evaluated the cardiac conditions by alkaline phosphatase staining,western blot analysis for apoptosis related proteins and signal pathways.Results MSC,especially AP- MSC increased fractional shortening (FS) of diabetic heart (P ＜0.01 vs DMEM respectively).AP-MSC greatly increased the capillary density of diabetic myocardium (P ＜0.01 vs DMEM and MSC group respectively).AP-MSC are anti-apoptotic in the rat DCM model,possibly mediated through cardiac upregulation of Bcl-2/Bax ratio ( P ＜ 0.05 ) and inhibiting the expression and activation of caspase - 3 ( P ＜ 0.01 ).Conclusions Intramyocardial transplantation of APMSC has a protective effect on diabetic cardiomyopathy.

To investigate the therapeutic effect of peroxiredoxin-6(PRDX6)on ultraviolet-induced corneal injury in rats and explore the mechanism.The rat model of corneal injury was established by exposing to ultravio-let.Male wister rats were randomly divided into control groups,dexamethasone (DXM)groups and PRDX6 groups,the rats were administered four times a day and for 12 days.The corneal opacity was observed with a slit-lamp microscope.Histopathologic changes were observed with light microscopy.The content of corneal malonalde-hyde(MDA)was determined by thiobarbituric acid test and the total antioxidative capacity(TAOC)was detected by chemical colorimetric test.P38 MAPK signal pathway was detected with the method of Western blot and the gene expression of cytokines were measured by RT-PCR method.Compared with the control group,PRDX6 treat-ment significantly reduced corneal opacity,improved corneal pathology injury,decreased the MDA content and in-creased the TAOC.In the PRDX6 group the level of phosphorylated p38 protein was significantly lower than that in the control group.The gene expression of cytokine were different between control and PRDX6 groups(P <0.05).PRDX6 showed therapeutic effect in the rat model of ultraviolet-induced corneal injury.This maybe be concerned with that it could alleviated the oxidative damage,suppressed p38 MAPK phosphorylation and regulate the gene expression of cytokine.

BACKGROUND:With the social and economic development and aging, coronary heart disease has become the primary cause of death endangering human life and health, while cardiovascular intervention (especial y stenting) in the treatment of cardiovascular disease is at a growing status. OBJECTIVE:To describe the development, research progress and latest clinical application of heart interventional materials as wel as advantages and disadvantages of biodegradable stents, and meanwhile to prospect for its future development and improvement. METHODS:A computer-based online search was conducted in PubMed for English language publications from January 1999 to April 2014 using the key words of“stent, scaffold, bioabsorbable, bioresorbable, biodegradable, biocompatibility, material properties”in English. RESULTS AND CONCLUSION:The new ful y biodegradable stents are considered as another major progress in the cardiac intervention, which brings a new gospel for patients with coronary heart disease. In the initial stage of implantation, biodegradable stents exhibit the same mechanical support as bare metal stents;after implantation, anti-proliferative drugs are released to prevent thrombosis and restenosis, and the stent is completely degraded within a specified period, which significantly reduces the late-stage and very late-stage thrombosis and the risk of in-stent restenosis. However, long-term safety and efficacy of biodegradable stents need further studies. Innovation of stent design and materials science is the key to overcome the current shortcomings of biodegradable stents.

Objective To evaluate the image deformation and measurement error of rotational DSA by model experiment. Methods Five steel balls (diameter: 20 mm) were placed in a horizontal space of 30 mm, the middle of which was as the isocenter for rotational DSA acquisition. Another 5 steel balls (diameter: 10 mm) were placed in a vertical space of 30 mm with the same alignment for rotational DSA acquisition. The screen was divided into five regions and the morphological changes of the ball image were observed at each rotation angle. The experimental images of the left anterior oblique ball images at 20°, 40°and 60° among each two groups were selected and measured by automatic isocenter calibration and sphere calibration respectively, and compared with the actual diameters of balls to calculate the image magnification. Results Except the center one, other balls were of deformation at different rotation angles due to the distance to the detector leading to enlargement and reduction. With automatic isocenter calibration, only the diameter of the center ball corresponded with the actual one, the distance away from the center or the rotation angle was inversely proportional to measurement error (maximum magnification 12.42%).With sphere calibration, the measurement results in various regions at different rotation angle were basically the same, with smaller measurement error (maximum magnification 3.41%). Conclusion Placing the organ of interest in the center area can reduce image distortion in rotational DSA imaging. Selecting reasonable measuring technology according to the lesion location is helpful to control measurement errors.

Background and purpose: MORC2 (microrchidia family CW-type zinc finger 2, MORC2) is a newly identified chromatin remodeling protein that plays key roles in DNA-based biological processes including gene transcription and DNA damage repair. However, its functional role in breast cancer development and progression re-mains unknown. ALDH1A3 (aldehyde dehydrogenase 1 family member A3), a member of the aldehyde dehydrogenases (ALDH) superfamily, is a putative breast cancer stem cell marker, but its regulatory mechanism in breast cancer is poor-ly characterized. This study aimed to investigate the effects of knockdown of endogenous MORC2 on the expression levels of ALDH1A3 and the breast cancer stem-like phenotype in MCF-7 cells. Methods: Human breast cancer MCF-7 cells were infected with negative control short hairpin RNAs (shNC) and specific shRNAs targeting human MORC2 (shMORC2), followed by selection with puromycin to generate stable MORC2 gene knockdown cell lines. Western blot and real-time fluorescent quantitative polymerase chain reaction (RTFQ-PCR) were used to examine the protein and mRNA levels of ALDH1A3 in MCF-7 cells stably expressing shNC and shMORC2. Microsphere formation and fluo-rescence-activated cell sorting (FACS) assays were used to analyze the effects of knockdown of MORC2 on the breast cancer stem-like phenotype. Results: Western blot and RTFQ-PCR analyses revealed that the protein and mRNA levels of ALDH1A3 were significantly down-regulated in shMORC2 expressing cells as compared with shNC -transfected control cells. Moreover, mammosphere formation assay showed that knockdown of endogenous MORC2 in MCF-7 cells significantly reduced the ability of cells to form microspheres. Consistently, FACS assays demonstrated that shMORC2-transfected cells had a lower proportion of ALDH-positive stem cells as compared with shNC expressing cells. In contrast, knockdown of MORC2 did not significantly affect the CD44+CD24- stem cell population. Conclusion:MORC2 promotes a breast cancer stem-like phenotype through, at least in part, regulating ALDH1A3 expression.

Objective:To investigate the balance of Th1/Th2 and Tc1/Tc2 in peripheral whole blood from SLE patients and find out the relationship between disease activity and the balance of type 1 and type 2 cytokines.Methods:Peripheral blood cells from SLE patients(active,n=15,inactive,n=20) were stimulated and normal subjects(n=20) with phorbol myristate acetate and ionomycin(PMA/I) for 4 h,then IFN-? and IL-4 in CD3~+CD8~-T cells(Th) and CD3~+CD8~+T cells(Tc) were detected by recently developed intracytoplasmic cytokine-staining techniques,anti-dsDNA antibodies in serum was detected with enzyme linked immunosorbant assay(ELISA) and immunoglobulin in serum and protein in urine were analyzed with rate nephelometry.Results:In SLE patients with active disease,the percent of IFN-?-secreting Th cells significantly increased compared with SLE patients with inactive disease and normal after in vitro PMA/I stimulation,the percent of IFN-?-secreting Tc cels significantly increased compared with normal,but IL-4-secreting Th cells and Tc cells did not vary significantly.In SLE patients with inactive diseases,only the percent of IFN-?-secreting Tc cells significantly increased compared with control.The percent of IFN-?-secreting Th and Tc cells in patients with anti-dsDNA antibody(+) in serum decreased significantly compared with patients with anti-dsDNA antibody(-).Although the percent of IL-4-secreting Th and Tc cells in patients with aberrantly high level of immunoglobulin had no change,but the mean fluorescent intensify(MIF) of IL-4-secreting Th and Tc cells increased significantly compared with patients with normal level of immunoglobulin.There was no correlation between the percent of IFN-? or IL-4 in Th or Tc cells and the content of protein in patients' urine.Conclusion:The balance between type 1 and type 2 cytokine-secreting T cells is disrupted in SLE patients,especially IFN-?-secreting T cells,which is significantly in favour of Th2 cells.The level of IFN-? or IL-4 in Th or Tc cells varied partly with disease activity,which deserved further study.