Objective To investigate the relationship between body mass index(BMI) and the outcomes of liver function of patients with hepatocarcinoma after surgery. Methods We divided the patients into three groups: normal, overweight and obese groups based on BMI, then we recorded their preoperative and the postoperative liver functions: albumin, total bilirubin, alanine aminotrasferase(ALT), aspertate aminotransferase (AST), alkaline phosphatase(ALP), γ-glutamyltranoferase(γ-GGT), triglycerides(TG), total cholesterol (TC), high density lipoprotein(HDL) and low density lipoprotein(LDL). Results The mean values of ALT change between the preoperative and the postoperative were (14.9 ± 1.98) U/L, (15.4 ± 2.27) U/L,(22.2±1.32) U/L; Mean values of γ-GGT change were(16.2 ± 1.33) U/L, (18.7 ±0. 97) U/L,(22.3 ± 1.27) U/L; mean values of TG change were (0. 18 ± 0. 44) mg/dL, (0. 21 ± 0.08) mg/dL,(0.25 ± 0.03) mg/dL. Compared with the results of control groups, there were no significant differences of recovery of liver functions between normal and overweight groups(P ＞ 0. 05), but the obese group' s recovery of liver functions did lag behind the normal group (P ＜ 0.05). It is showed that the obese group' s shared same recovery of albumin, total bilirubin, AST, ALP, TC, HDL and LDL. The difference was significant between the normal and obese groups in terms of recovery of ALT, γ-GGT, TG(P ＜ 0. 05). Conclusion BMI is associated with the recovery of liver function of patients with hepatocarcinoma, the obese patients have poor prognosis of recovery of liver function.

Objective To investigating the application of serum carbohydrate antigen 199(CA199) level increase in the patients with different characters of obstructive jaundice .Methods 21 patients with benign obstructive jaundice (benign group) and 24 pa‐tients with malignant obstructive jaundice (malignant group) were selected .The CA199 level in these two groups was higher than the reference value .Serum CA199 level was all higher than average value .The changes of serum CA199 level and direct bilirubin (DBIL) before and after treatment were dynamically monitored and their correlation was analyzed .Results There was no statisti‐cally significant difference in serum CA199 and DBIL levels before treatment between the benign group and the malignant group (P> 0 .05) .The serum CA199 and DBIL levels after treatment in the benign group were obviously decreased compared with before treatment ,the difference was statistically significant (P< 0 .05) ,however ,which in the malignant group had no statistical difference between before and after treatment (P > 0 .05) .Conclusion CA199 is not a marker of very high tumor specificity ,and could not serve as the sole marker for judging benign and malignant diseases .Dynamically monitoring the change of serum CA199 before and after treatment and its correlation with DBIL is conducive to differentiate between benign with malignant diseases .

Objective: Interleukin-10 (IL-10) is an anti-inflammatory helper T cell type 2 (Th2) cytokine that modulates Th1-type cytokine production. The purpose of present study was to detect the expression and changes of IL-10 in the rats heart transplantation, as determine the role of IL-10 in the acute rejection of rats heart transplantation. Methods: Hearts from DA rats were transplanted into the necks of Wistar recipients. Allografts was divided into five groups: group A (control, n=12), group B (DST treated, n=12), group C (IL-2 Mab treated, n=12), group D (IL-4 Mab treated, n=12), group E (IL-2 Mab treated, n=12). The local expression of IL-10 was analyzed at day 1,3,5,7,9,11,14 by reversed transcripition polymerase chain reaction (RT-PCR). Results: Allografts survival time was (8.3±1.7) days, (29.2±7.1) days, (26.4±5.7) days, (10.2±1.9) days and (55.0±10.6) days (P＜0.05， P＜0.01), respectively. When compared with group A, the level of IL-10 was upregulated in group B, group C and group E, especially in group E. The upregulation of IL-10 was related to the grafts survival time. Conclusion: IL-10 plays an important role in the development of acute transplantational rejection, suggests that Th-2 type deviation may contribute to the prolongation of graft survival.

BACKGROUND: Recently, researchers separate neural stem cells (NSCs) from neural system. As the progenitor of nerve cell and glia cells, NSCs have strong ability of self-renewal and proliferation, and play a very important role in repair process after nerve injury. OBJECTIVE: To review the repairing effect of NSC transplantation on hypoxic-ischemic encephalopathy. RETRIEVAL STRATEGY: Using the "Neural stem cells, brain, hypoxic-ischemic", we searched PubMed database for related articles published in English between 1993 and 2007. 131 articles were collected, and after the first trial, only articles ① related to neural stem cell transplantation for brain injury, and ②published in recently or in authoritative journals were selected. Repetitive researches were excluded. LITERATURE EVALUATION: The articles were about the studies of NSCs and randomized and controlled experiment for hypoxic-ischemic brain injury. Thirty-three English articles were included involving the biological characteristics of NSC, distribution, separation and culture in vitro, and the basic experiment or clinical research about the repair of hypoxic-ischemic brain injury by NSC. Of these articles, there are 7 review articles, and the others were about clinic or basic experiment researches. DATA SYNTHESIS: Researches show that after transplantation NSC can survive in host, furthermore, it proliferates and differentiates to repair the configuration and function of injured brain area. Even though we have achieved much in NSC transplantation for brain injury, yet there are many problems to be resolved, such as NSC source and conservation, the control mechanism of differentiation and proliferation when NSC is cultured in vitro. In addition, the amount or functional activity of NSCs acquired is lower, and the molecular biological features of cells in repaired tissue are uncertain. CONCLUSION: As a kind of ideal seed cell, NSC provides an approach for the repair of hypoxic-ischemic brain injury, and starts a new era of clinic therapy by stem cells.

Objective To evaluate the effect of pantoprazole and norepinephrine injection on serum levels of inflammatory factors and hemostasis in the treatment of patients with peptic ulcer bleeding .Methods 102 cases of patients with peptic ulcer bleeding from September 2014 to September 2016 in our hospital were selected and randomly divided into observation group ( n =51 ) and control group ( n =51 ) .Observation group were received pantoprazole and norepinephrine injection combination therapy, the control group received only pantoprazole therapy.The treatment lasted 3 days.The efficacy, bleeding time, hospitalization and blood transfusion, serum IL-6 and IL-10 levels, the incidence of rebleeding and incidence of adverse reactions were compared between two groups.Results The overall response rate ( 98.02%) was significantly higher ( 82.35%) ( P<0.05 );observation group bleeding time was significantly faster than the control group ( P<0.05 ) , length of hospital stay was significantly shorter than the control group (P<0.05), blood transfusion was significantly less than the control group (P<0.05); levels of serum IL-6, IL-10 after treatment were lower than those before treatment (P<0.05); the serum levels of IL-6, IL-10 after treatment were significantly lower than those in control group (P<0.05); rebleeding rate in observation group was significantly lower than that in control group (P<0.05);there was no significant difference in adverse reactions between two groups.Conclusion Pantoprazole and norepinephrine injection in the treatment of digestive bleeding ulcer bleeding has the obvious effect, can reduce serum levels of inflammatory factors, while also reduce the incidence of rebleeding, safe and reliable.

Objective: To determine magnolol and honokiol in Huoxiangzhengqi Capsule(oil of Herba Agastachis, oil of Perillae, Pericarpium Citri Reticulatae, Cortex Magnoliae Officinalis, etc.).Methods: A supercritical Fluid Extraction (SFE) has been used to extract magnolol and honokiol in Huoxiangzhengqi Capsule, and CGC was used to analyse the extracts.Results: The average rccovery was 96.81% ( RSD =2.81%, n =5) for magnolol, and 97.75% ( RSD =2.36%, n =5) for honokiol, respectively. Conclusion: The SFE CGC offline method for the analysis of Huoxiangzhengqi capsule is simple, quick and accurate.

The examinations of optical coherence tomography (OCT) images were obtained in 136 patients. Lactated Ringer's solution or physiological saline was used randomly as perfusate. Their electrocardiogram and cardiac enzymes were acquired to compare the safety and image quality of two perfusates.The incidence of ventricular arrhythmia and rise of cardiac enzymes were lower in the lactated Ringer's solution group than those in the physiological saline group.Yet the image quality of OCT had no difference.The use of lactated Ringer's solution as perfusate instead of physiological saline in OCT examination may protect the ischemic myocardium and reduce the incidence of complications.

In 1989, Fitz defined and classified severe acute pancreatitis (SAP) and he thought that surgical treatment of SAP is feasible. Since then, the strategy of treating SAP was a controversial issue over past decades. Currently, relevant literatures reported that medical or minimally invasive treatments are superior to surgical treatment, while it is too early to make the final conclusion because of the complexity of SAP. From 1989 to 2008, 1852 patients with acute pancreatitis were treated at the General Hospital of PLA, and the clinical data of 18 patients who died of SAP were retrospectively analyzed. Based on the analysis, we concluded that medical conservative therapy and surgical operative therapy should not be opposed to each other. Selecting ideal timing and appropriate operation on the basis of pathological changes of retroperitoneum and pancreatitis should be considered seriously, and the new concept of "miniaturization of trauma" should also be introduced in treating SAP.

Objective Through studying the apoptosis induced by stichopus japonicus acid mucopoly saccharide in the hepatocellular carcinoma cell line HepG2 in vitro, analysing the expression of Bcl-2 and nm-23in HepG2, to provide the theory foundation and its feasibility on whether it can be used for the chemotherapy of hepetocellular carcinoma. Methods The cells of HepG2 were cultured in vitro and treated with SJAMP at different doses(0.25,0. 5,1.0,2.0,4.0 g/L). MTT was used to observe the inhibitory effects of SJAMP on cell growth, Western blotting was used to detect apoptosis, and the apoptosis related change of expression of protein Bcl-2 and nm23-H1. Results (1) MTT identified that SJAMP produced an obvious time-and-dose-dependent inhibitory effect on the HlepG2 cells. (2) Western blot showed that SJAMP could induce the apoptosis of HepG2 cells through changing the expression of the protein of Bcl-2 and nn23-H1 (P<0.05). Conclusion (1)SJAMP produced obvious inhibitory effects on HepG2 cells and induce HepG2 apoptosis. (2)SJAMP can enduce the anti-tumor function in the method of changing the expression of protein Bcl-2 and nm23-H1.

Objective To analyze the clinical characteristics and therapeutic effects of cutaneous metastasis from cholangiocarcinoma. Methods From 2006 to 2009, clinical data of 6 patients found with cutaneous metastasis from cholangiocarcinoma were reported. Perineural and vascular invasion were all found in the primary cholangiocarcinoma in these 6 cases with cutaneous metastasis and results were compared with 60 cases of cholangiocarcinoma without cutaneous metastasis. Follow-up to the 6 patients with cutaneous metastases was conducted at an interval of three months. Results One patient with mixed hepatocellular and cholangiocellular carcinoma developed cutaneous metastatic tumor and the pathological diagnosis was cholangiocellular carcinoma. Three patients with hilar cholangiocarcinoma and two patients with distal cholangiocarcinoma presented cutaneous metastases. All six cases of the primary cancers and cutaneous metastases were diagnosed as moderately- poorly differentiated adenocarcinoma. And there was perineural and vascular invasion in the primary tumors. Perineural and vascular invasion of primary cancer was found in 17of 60 patients with cholangiocarcinoma and without cutaneous metastasis. The cutaneous metastasis was positively correlated to the perineural and vascular invasion in the primary tumor (χ2 = 12. 7288, P ＜0. 001 ). Two of 6 cases presenting solitary nodular cutaneous metastasis received skin tumor excision. These two patients survived 8 and 10 months respectively. Among four patients with multiple cutaneous metastases,two cases received chemotherapy and survived 3 and 6 months respectively, the other two cases refusing treatment survived 3 and 4 months respectively. Conclusions Cutaneous metastasis usually occurred at cholangiocarcinoma with lower differentation and perineural and vascular invasion in the primary tumor.Solitary nodular cutaneous metastasis could be excised, multiple cutaneous metastases had poor prognosis.