Objective To investigate the relationship between body mass index(BMI) and the outcomes of liver function of patients with hepatocarcinoma after surgery. Methods We divided the patients into three groups: normal, overweight and obese groups based on BMI, then we recorded their preoperative and the postoperative liver functions: albumin, total bilirubin, alanine aminotrasferase(ALT), aspertate aminotransferase (AST), alkaline phosphatase(ALP), γ-glutamyltranoferase(γ-GGT), triglycerides(TG), total cholesterol (TC), high density lipoprotein(HDL) and low density lipoprotein(LDL). Results The mean values of ALT change between the preoperative and the postoperative were (14.9 ± 1.98) U/L, (15.4 ± 2.27) U/L,(22.2±1.32) U/L; Mean values of γ-GGT change were(16.2 ± 1.33) U/L, (18.7 ±0. 97) U/L,(22.3 ± 1.27) U/L; mean values of TG change were (0. 18 ± 0. 44) mg/dL, (0. 21 ± 0.08) mg/dL,(0.25 ± 0.03) mg/dL. Compared with the results of control groups, there were no significant differences of recovery of liver functions between normal and overweight groups(P ＞ 0. 05), but the obese group' s recovery of liver functions did lag behind the normal group (P ＜ 0.05). It is showed that the obese group' s shared same recovery of albumin, total bilirubin, AST, ALP, TC, HDL and LDL. The difference was significant between the normal and obese groups in terms of recovery of ALT, γ-GGT, TG(P ＜ 0. 05). Conclusion BMI is associated with the recovery of liver function of patients with hepatocarcinoma, the obese patients have poor prognosis of recovery of liver function.

Objective To investigating the application of serum carbohydrate antigen 199(CA199) level increase in the patients with different characters of obstructive jaundice .Methods 21 patients with benign obstructive jaundice (benign group) and 24 pa‐tients with malignant obstructive jaundice (malignant group) were selected .The CA199 level in these two groups was higher than the reference value .Serum CA199 level was all higher than average value .The changes of serum CA199 level and direct bilirubin (DBIL) before and after treatment were dynamically monitored and their correlation was analyzed .Results There was no statisti‐cally significant difference in serum CA199 and DBIL levels before treatment between the benign group and the malignant group (P> 0 .05) .The serum CA199 and DBIL levels after treatment in the benign group were obviously decreased compared with before treatment ,the difference was statistically significant (P< 0 .05) ,however ,which in the malignant group had no statistical difference between before and after treatment (P > 0 .05) .Conclusion CA199 is not a marker of very high tumor specificity ,and could not serve as the sole marker for judging benign and malignant diseases .Dynamically monitoring the change of serum CA199 before and after treatment and its correlation with DBIL is conducive to differentiate between benign with malignant diseases .

Objective To evaluate the effect of pantoprazole and norepinephrine injection on serum levels of inflammatory factors and hemostasis in the treatment of patients with peptic ulcer bleeding .Methods 102 cases of patients with peptic ulcer bleeding from September 2014 to September 2016 in our hospital were selected and randomly divided into observation group ( n =51 ) and control group ( n =51 ) .Observation group were received pantoprazole and norepinephrine injection combination therapy, the control group received only pantoprazole therapy.The treatment lasted 3 days.The efficacy, bleeding time, hospitalization and blood transfusion, serum IL-6 and IL-10 levels, the incidence of rebleeding and incidence of adverse reactions were compared between two groups.Results The overall response rate ( 98.02%) was significantly higher ( 82.35%) ( P<0.05 );observation group bleeding time was significantly faster than the control group ( P<0.05 ) , length of hospital stay was significantly shorter than the control group (P<0.05), blood transfusion was significantly less than the control group (P<0.05); levels of serum IL-6, IL-10 after treatment were lower than those before treatment (P<0.05); the serum levels of IL-6, IL-10 after treatment were significantly lower than those in control group (P<0.05); rebleeding rate in observation group was significantly lower than that in control group (P<0.05);there was no significant difference in adverse reactions between two groups.Conclusion Pantoprazole and norepinephrine injection in the treatment of digestive bleeding ulcer bleeding has the obvious effect, can reduce serum levels of inflammatory factors, while also reduce the incidence of rebleeding, safe and reliable.

Objective: Interleukin-10 (IL-10) is an anti-inflammatory helper T cell type 2 (Th2) cytokine that modulates Th1-type cytokine production. The purpose of present study was to detect the expression and changes of IL-10 in the rats heart transplantation, as determine the role of IL-10 in the acute rejection of rats heart transplantation. Methods: Hearts from DA rats were transplanted into the necks of Wistar recipients. Allografts was divided into five groups: group A (control, n=12), group B (DST treated, n=12), group C (IL-2 Mab treated, n=12), group D (IL-4 Mab treated, n=12), group E (IL-2 Mab treated, n=12). The local expression of IL-10 was analyzed at day 1,3,5,7,9,11,14 by reversed transcripition polymerase chain reaction (RT-PCR). Results: Allografts survival time was (8.3±1.7) days, (29.2±7.1) days, (26.4±5.7) days, (10.2±1.9) days and (55.0±10.6) days (P＜0.05， P＜0.01), respectively. When compared with group A, the level of IL-10 was upregulated in group B, group C and group E, especially in group E. The upregulation of IL-10 was related to the grafts survival time. Conclusion: IL-10 plays an important role in the development of acute transplantational rejection, suggests that Th-2 type deviation may contribute to the prolongation of graft survival.

BACKGROUND: Recently, researchers separate neural stem cells (NSCs) from neural system. As the progenitor of nerve cell and glia cells, NSCs have strong ability of self-renewal and proliferation, and play a very important role in repair process after nerve injury. OBJECTIVE: To review the repairing effect of NSC transplantation on hypoxic-ischemic encephalopathy. RETRIEVAL STRATEGY: Using the "Neural stem cells, brain, hypoxic-ischemic", we searched PubMed database for related articles published in English between 1993 and 2007. 131 articles were collected, and after the first trial, only articles ① related to neural stem cell transplantation for brain injury, and ②published in recently or in authoritative journals were selected. Repetitive researches were excluded. LITERATURE EVALUATION: The articles were about the studies of NSCs and randomized and controlled experiment for hypoxic-ischemic brain injury. Thirty-three English articles were included involving the biological characteristics of NSC, distribution, separation and culture in vitro, and the basic experiment or clinical research about the repair of hypoxic-ischemic brain injury by NSC. Of these articles, there are 7 review articles, and the others were about clinic or basic experiment researches. DATA SYNTHESIS: Researches show that after transplantation NSC can survive in host, furthermore, it proliferates and differentiates to repair the configuration and function of injured brain area. Even though we have achieved much in NSC transplantation for brain injury, yet there are many problems to be resolved, such as NSC source and conservation, the control mechanism of differentiation and proliferation when NSC is cultured in vitro. In addition, the amount or functional activity of NSCs acquired is lower, and the molecular biological features of cells in repaired tissue are uncertain. CONCLUSION: As a kind of ideal seed cell, NSC provides an approach for the repair of hypoxic-ischemic brain injury, and starts a new era of clinic therapy by stem cells.

Objective: To determine magnolol and honokiol in Huoxiangzhengqi Capsule(oil of Herba Agastachis, oil of Perillae, Pericarpium Citri Reticulatae, Cortex Magnoliae Officinalis, etc.).Methods: A supercritical Fluid Extraction (SFE) has been used to extract magnolol and honokiol in Huoxiangzhengqi Capsule, and CGC was used to analyse the extracts.Results: The average rccovery was 96.81% ( RSD =2.81%, n =5) for magnolol, and 97.75% ( RSD =2.36%, n =5) for honokiol, respectively. Conclusion: The SFE CGC offline method for the analysis of Huoxiangzhengqi capsule is simple, quick and accurate.

The examinations of optical coherence tomography (OCT) images were obtained in 136 patients. Lactated Ringer's solution or physiological saline was used randomly as perfusate. Their electrocardiogram and cardiac enzymes were acquired to compare the safety and image quality of two perfusates.The incidence of ventricular arrhythmia and rise of cardiac enzymes were lower in the lactated Ringer's solution group than those in the physiological saline group.Yet the image quality of OCT had no difference.The use of lactated Ringer's solution as perfusate instead of physiological saline in OCT examination may protect the ischemic myocardium and reduce the incidence of complications.

Objective: To establish a method for the determination of quercetin and kaempferol in Ginkgo biloba extract. Methods: A simple, rapid and reproducible supercritical fluid extract (SFE) and capillary electrophoretic method were developed for it. SFE was selected by orthogonal design method. SFE conditions: temperature 60 ℃, pressure 42 mPa, static extraction 4 min, dynamic extraction volume 4 ml and 0.2 ml ethanol as modifier. MECC conditions were 55 cm?75 ?m fused silica capillary column. The running buffer contained 25 mmol/L sodium dihydrophosphate, 6.25 mmol/L sodium borate (pH= 8.5) and 35 mmol/L sodium dodecyl sulfate(SDS). The detection wave length was at 254 nm. Results: The influence of modifier on extraction rate in SFE was the most. The linear range for quercetin and kaempferol was 21.2 106.0, 20.0 100.0 ?g/ml, respectively, when cinnamic acid was in an internal standard. The recovery of quercetin for 40, 60 and 80 ?l was 93.87%, 94.02% and 94.10%, respectively. The recovery of kaempferol for 40, 60 and 80 ?l was 94.50%, 94.17% and 94.25%, respectively. Conclusion: SFE MECC is convenient and accurate method, and can be used as a measure of quality control for Ginkgo biloba extract. [

Objective To assess the feasibility of hepatolithiasis treatment using laparoscopic liver resections. Methods A prospective study of laparoscopic liver resections was undertaken in selected patients with left intrahepatic stone, and these patients were operated by laparoscopic left lobectomy. Results Two cases were operated successfully. Mean blood loss was 400～600 ml. Conclusion Laparoscopic resections are feasible in selected patients with left intrahepatic stone and bring up a new method for hepatolithiasis.

Objective To investigate the effects of structured triglyceride (STG) and physical mixed medium chain/long chain triglycerides (MCT/LCT) on hepatic and renal function and lipometabolism of patients with acute necrotizing pancreatitis (ANP).Methods The clinical data of 30 patients with ANP who were admitted to the PLA General Hospital between January 2012 and June 2014 were prospectively analyzed.A double-blind,randomized,controlled study was performed in 30 patients who were allocated into the experimental group (15 patients received STG) and the control group (15 patients received physical mixed MCT/LCT).All the patients received isometrical nitrogen and isocaloric parenteral nutrition more than 5 days.The levels of alanine transaminase (ALT),aspartate transaminase (AST),glutamyl-transpeptidase (GGT),alkaline phosphatase (ALP),creatinine (Cr),blood urea nitrogen (BUN),triglyceride (TG) and total cholesterol (TC) were assayed before nutritional support treatment and at day 1,3 and 5 after nutritional support therapy.The measurement data with normal distribution was presented as (x) ± s.The skew distribution data were described as M (range).The comparison between groups were evaluated with an independent sample t test or one-way ANOVA.The count data were analyzed using the chi-square test.Results A total of 30 patients were screened for eligibility.The levels of ALT,AST,GGT,ALP,Cr,BUN,TG and TC were changed within a certain range at day 1,3 and 5 after nutritional support treatment.The levels of ALT,AST,GGT,ALP,Cr,BUN and TC before treatment and at day 5after treatment were changed from 29.0 U/L,25.4 U/L,83.2 U/L,(193 ± 115) U/L,(124 ± 97) μmol/L,(8±6)mmol/L and (2.4±1.1)mmol/L to 29.4 U/L,33.0 U/L,77.7 U/L,(172±74)U/L,(117 ±103)μmol/L,(8 ± 5) mmol/L and (2.3 ± 1.0) mmol/L in the experimental group,and from 23.8 U/L,22.9 U/L,96.2 U/L,(148 ± 108) U/L,(82 ± 57) μmol/L,(9 ± 7) mmol/L and (2.5 ± 0.7) mmol/L to 21.3 U/L,24.5 U/L,127.4 U/L,(179 ± 126) U/L,(80 ± 54) μmol/L,(10 ± 6) mmol/L and (2.4 ±0.8) mmol/L in the control group,respectively.There were no significant differences in the changing trends of the levels of ALT,AST,GGT,ALP,Cr,BUN and TC between the 2 groups (F =0.647,1.186,0.282,0.553,0.862,0.182,0.369,P＞0.05).The level of TG in the experimental group from pre-treatment to day 5 after treatment was changed from (1.5 ± 0.6) mmol/L to (1.5 ± 0.7) mmol/L,with increasing trend from pre-treatment to day 1 after treatment and reaching the normal level at day 3 and 5 after treatment.The level of TG in the control group from pre-treatment to day 5 after treatment was changed from (1.5 ± 0.6) mmol/L to (2.4 ± 0.6) mmol/L,with increasing trend from pre-treatment to day 1,3 and 5 after treatments.There were significant differences in the changing trends of TG before and after nutritional support therapy between the 2 groups (F =7.940,P ＜ 0.05).Conclusion STG and physical mixed MCT/LCT don't influence the hepatic and renal function of patients with ANP undergoing parenteral nutritional support therapy,while STG has a better effect of lipometabolism compared with physical mixed MCT/LCT.Registry This study was registered with the UMIN Clinical Trial Registry with the registry number of UMIN000016958