ObjectiveTo study the potential of using temporal clustering analysis(TCA)technique in localizing an epileptogenic zone.MethodsTwelve patients with epilepsy were examined using resting functional MRI(fMRI). The patients had detectable focal lesions on cranial MRI.TCA was performed to analyze resting fMRI data in order to identify the timing of interictal epileptiform discharges (IEDs).Standard event-related fMRI analysis in SPM99 was used to generate maps of the activation induced by epileptic brain activities.Comparisons were made between TCA Resultsand SPM motion trochoid.ResultsEight of the twelve subiects showed activations in the brain regions that were consistent with those lesions determined on anatomic MRI.The remaining four subiects showed no clear activation in the areas of detectable lesions. In addition, correlation was found between TCA Resultsand motion trochoids.ConclusionsTemporal cluster analysis,an exploratory data-driven technique,may provide the timing information about interictal epileptiforill discharges.However,the Resultsfrom this novel fMRI analytical technique need to be interpreted with caution as it is vulnerable to motion artifact.

Objective To explore the short and long term result and the impact on cell immunity of splenic radiofrequence ablation in patients with hypersplenism.Methods In this study 43 patients were divided into the RFA treatment group(22 cases)and open splenectomy control group(21 cases).Blood samples were collected for blood routine,lymphocyte transformation efficiency,NK cell activity and T lymphocyte subpopulations at 1 week,1 month,6 month,1 year,2 years and 3 years before and after operation respectively and the results were compared between the two groups.Results The hypersplenism of the patients in both groups were relieved.There were no significant difierences in cell indices between the postoperative and preoperative values in the two groups.In the control group,compared with the preoperative values in the trial group,the postoperative NK cell activity and lymphocyte transformation efficiency were significantly reduced(P＜0.05).Even though,with time,the NK cell activity and lymphocyte transformation efficiency were on an increasing tendency after operation but still significantly lower than that before operation.In the control group,compared with the preoperative values,CD4+T cell count and CD4+/CD8+ratio decreased significantly(P＜0.05).Moreover.with time.these indices were on an increasing tendency.CIM+T cell count and CD4+/CD8+ ratio restored to the preoperative values within 30 months(P=0.078)and 36 months(P=0.103)respectively.Conclusion RFA treatment for hypersplenism preserves a part of the spleen and thus preserves patient's cell immunity.This treatment exhibits microinvasiveness and few complications and being easy to operate.Hence,RFA treatment for hypersplenism merits wide clinical application.

Objective To investigate the effects of early hemofiltration combined with traditional Chinese medicine on nasal feeding on severe acute pancreatitis (SAP) patients. Methods Seventy four patients were divided into traditional Chinese medicine group (group A, 35 cases) and traditional Chinese medicine+hemofiltration group (group B, 39 cases). In group A, patients were given a serious of procedure including fasting, gastrointestinal decompression, fluid resuscitation, inhi-bition of pancreatic secretion, antibiotic prophylaxis, parenteral nutrition and traditional Chinese medicine on nasal feeding;In group B, patients received continuous veno-venous hemofiltration treatment(also called Continuous Renal Replacement Therapy,CRRT) in addition to the procedures receiving by group A. On admission and the first, 3rd, 7th days post-treatment, the scores of acute physiology and chronic health evaluation (APACHEⅡ), tumor necrosis factor-α(TNF-α), interleukin-6 (IL-6), interleukin-8 (IL-8) levels were recorded. Length of hospital stay, local and systematic complications, surgical inter-vention, mortality and hospitalization expenses were compared between two groups. Results On admission, no statistical significance was seen in the hematocrit, white blood cell count, lactic acid dehydrogenase, blood urea nitrogen, blood glu-cose, APACHEⅡscore, Ranson’s score and classification of etiology between these two groups (P>0.05). But APACHEⅡ, TNF-α, IL-6, IL-8 were decreased significantly in group B than in group A, after the first, 3rd, 7th days post-treatment (P＜0.05). Compared with group A, the length of hospital stay, local complications, systemic complications, surgical interven-tion, mortality and hospitalization expense were lower in group B. Conclusion Traditional Chinese medicine on nasal feed-ing combined with early hemofiltration could effectively reduce complications, incidence of organ dysfunction and could im-prove the prognosis of SAP patients.

By means of joint research of new advanced technology project,joint development and application of appropriate health technology project,comprehensive prevention and control of chronic diseases between municipal hospitals and community clinics project and research supporting platform project,Shanghai Hospital Development Center has effectively improved the clinical science and technology innovation capability, promoted the development of medical disciplines and talents and improved the discipline influence of such hospitals.

This paper introduced the design ideas and general information of Shanghai in building theHospital-Link Project.Thanks to innovative information technology and service model,it achieved real-time connectivity and clinical information sharing between the 38 municipal hospitals,which enables the general reform of such hospitals citywide.

Objective To assess the effectiveness and safety of non-insufflation intra-abdominal compression as a prehospital hemostatic technique in treatment of liver injury.Methods Twenty-nine male Wistar rats were enrolled and randomly assigned into four experimental groups:IAP0 group ( n =8 ),IAP5 group (n =8),IAP10 group (n =8) and IAP15 group (n =5).Then,the anticoagulated rat models of severe liver injury were established with different intra-abdominal pressures ( 0,5,10 and 15 mm Hg) by using a non-expansible insufflated gas bag.Once the mean artery pressure (MAP) was less than 95 mm Hg,Lactated Ringer' s solution was infused through the right jugular vein at speed of 3.3 ml · min-1 · kg-1 until MAP reached 100 mm Hg.Thirty minutes later,the animals were killed by intravenous injection of 0.2 ml saturated potassium chloride solution to measure the intraperitoneal total blood loss,liver weight,volume of infused solution and MAP.Results No death was observed in IAP0,lAP5 and IAP10 groups and four rats (4/5) died at 10-15 minutes following exposure to the 15 mm Hg intra-abdominal pressure in IAP15 group.Total blood loss was reduced to ( 54.20 ±11.30)ml/kg in IAP0 group,(43.98 ±9.2) ml/kg in IAP5 group,(32.49 ±7.40) ml/kg in IAP10group and (25.77 ± 14.16) ml/kg in IAP15group ( P ＜0.01 ).The infused volume of resuscitation solution was (31.06 ± 3.14) ml in IAP10 group,which was higher than (24.94 ±6.67) ml in IAP0 group,(23.06 ± 7.98) ml in IAP5 group and (16.50 ±7.27) ml in IAP15 group (P ＜0.05).Liver weight was ( 11.18 ± 1.45) g in IAP5 group,( 12.13 ± 0.96) g in IAP10 group and ( 11.41 ± 1.20) g in IAP15 group,which was significantly higher than (10.03 ±0.58) g in IAP0 group (P＜0.05).The MAP was (64.81 ± 19.65) mm Hg in IAP5 group and (65.80 ± 15.36) mm Hg in IAP10 group ( insignificant difference between groups,P ＞ 0.05 ),which was higher than (41.22 ± 10.00) mm Hg in IAP0 group and (44.50 ±28.60) mm Hg in IAP15 group (P ＜0.05).Conclusions Non-pneumatic intra-abdominal compression can effectively control bleeding in rat models of severe liver injury,while the adverse effect of intra-abdominal hypertension should be avoided.

The non-human primates have been profoundly being used to study the human reproductive models .It is the prerequisites to set up the operating rooms including hardware and software on reproductive experiments and transgenic manipulation.Thus, Combined with the practice of our center , both the construction and the associated problems about the IVF-ET operating rooms were discussed in this paper .It will provide some help for the related researches .

Objective To understand the distribution of Oncomelania hupensis snails in source areas of the east route of Southto-North Warter Diversion Project and evaluate the effects of the snails on the safety of water transfer.Methods The investigation of snail distribution was carried out in the source areas of the east route of South-to-North Warter Diversion Project every spring.The method of the random sample combined with environmental sample was used for the field investigation.The beach land in the stilling pool of Jiangdu Pumping Station was selected as a surveillance site to observe the snail spread.Results The areas of the snail habitats and infected snails were 256.11,184.55,164.92,121.16 hm~2 and 8.27,1.0,1.0,0 hm~2 respectively in the source areas of the east route of South-to-North Wafter Diversion Project from 2006 to 2009.The densities of living snails had a downtrend,too.Google Earth showed that the areas of snail habitats distributed mainly in the Jiajiang River and Mangdao River in the source areas.The snail habitats were detected in the beach land in the stilling pool of Jiangdu Pumping Station.The research results showed that the snail spread related to the wastes from the river of drawing water.Conclusion There are the risks of snail spread in the source areas of the east route of South-to-North Wafter Diversion Project,so that the long-term surveillance and control on the snails is very necessary.

Aim To determine the effective compo-nents of Semen Ziziphi Spinosae for sedative-hypnotic and its mechanism. Methods The extraction of Se-men Ziziphi Spinosae and the rat brain homogenates were prepared. High concentrations of Diazepam com-petitively replaced the ligand compounds of Semen Ziz-iphi Spinosae combining BDZ receptor in brain tissue, and all the compounds with sedative and hypnotic effects were collected and identified by HPLC and LC-MS technique, as the compounds extracted from the brain tissue were administered with Semen Ziziphi Spi-nosae. The brain tissue was administered with Diaze-pam, and with Semen Ziziphi Spinosae and Diazepam. Results The HPLC chromatograms show that the peak time of BDZ receptor ligand compounds was 2. 71 min and 46. 87min, when compared with Diazepam. And the LC-MS chromatograms display the relative molecu-lar weight of the ligand compounds was 274. 28 m/z, 453. 34 m/z,496. 34 m/z and 608. 38 m/z respective-ly. According to the fingerprint of Semen Ziziphi Spi-nosae, these compounds may be fatty acid substances and lupine pill triterpene compounds. Conclusions On the basis of the principle of receptor ligand bind-ing, we established a way to quickly analyze and iden-tify the role of natural products in the same drug target compounds. The method not only can clearly define the effective components of natural products, but also clar-ify the mechanism of action of the compounds. The ac-tive ingredient of calm hypnosis in Semen Ziziphi Spi-nosae may be fatty acid substances Palmitic acid ( C16 H32 O2 ) and lupine pill triterpene compounds Alphitolic acid( C30 H48 O4 ) and Spinosin( C28 H32 O15 ) . They exert their sedative and hypnotic effects by combining with BDZ receptor, and the research has laid a theoretical foundation for the further study about mechanism of Se-men Ziziphi Spinosae.

Objective To analyze the characteristics and changes of Oncomdania snail distribution in regions with different epidemic types and degrees of Jiangsu Province,so as to provide the evidence for implementing the feasible schistosomiasis control strategy.Methods A retrespeetive survey was performed,and the 5-year database of snail status in Jiangsu Province was established based on the collected reports of snail habitat cards and schistosomiasis control in Jiangsu Province from 2004 to 2008.The changes of snail areas,infected snail aroas,infection rates of snails in different years were analyzed,and tIIe annual vailations of snail areas,infected snail areas and infection rates of snails in areas with different epidemic types and degrees were eomparedI Results The actual snail areas and infected snail areas decreased by 3 799.86 hm~2 and 2 006.35 hm~2.respectively in Jiangsu Province.2006-2008.with the annual meaan decreasing rates of 9.64% and 18.83%.Among them,the actual snail areas in marshland and lake regions,water net regions and mountainous regions decreased by 3 546.10.209.19 hm~2 and 44.57 hm~2,respectively,with the annual meall decreasing rates of 9.59%,10.29% and 11.18%,respectively.An area of 22.02 hm~2 of new snail habitatswas detected during the 5 years,and thewater net regions accounted for 89.24%.Theproportions of actual snail areas in historical snail ale,as were between 2.77% and 5.36%.Among them.the proportions of actual snail areas in historical accumulative snail area8 in transmission-uncontrolled.trailsmission-controlled and transmission-interrupted areas were 13.29%-22.82%,0.88%-3.54% and 0.14%-0.32%,respectively,and there were significant differences among them(P<0.01).The constituent ratios of the actual snail areaswere 93.79%-95.60%.3.67%-5.45% and 0.73%-1.01%, respectively in marshland and lake regions,water net regions and mountainous regions from 2004 to 2008.The infection rate of snails in Jiangsu Province reduced from 0.15% in 2004 to 0.02% in 2008,with a reduction rate of 85.50%(P<0.01).The constituent ratios of infected snail areas in transmission-uncontrolled, transmission-controlled and transmission-interrupted areas were 98.15%-100%,0-0.81%, and 0-1.43%,respectively.Conclusions As a whole,there is a decreasing tendency of snail areas and infected snail areas in Jiangsu Province.and the snail control has been consohdated step by step in transmission-interrupted areas.The effective control of snails in marshland and lake regions and infected snails in transmission-uncontrolled areas are the key points in the future schistosomiasis control of Jiangsu Province.