Objective To explore the feasibility of laparoscopic subtotal colectomy on the basis of lesion identification with the combined use of laparoscopy and fibrocolonoscopy.Methods The operation was carried out under general anesthesia.The patients were maintained at a supine position.Four 10 mm trocars were introduced at the upper and lower borders of the umbilicus,and the left and right lower quadrants of the abdomen,respectively,and a 5 mm trocar was introduced at the right upper quadrant of the abdomen.The laparoscope was placed at the hypogastrium when performing right hemicolectomy,and at the left lower abdomen when left hemicolectomy.During the operation,a fibrocolonoscope was inserted by way of the severed end of the right colon for lesion identification.The colon was disconnected from the cecum to the sigmoid colon.Then the incision at the left lower abdomen was extended to 4 cm in length,and an extracorporeal ileosigmoidostomy was conducted. Results Pathologic changes of thickening and hardening intestinal walls were clearly observed under laparoscope.Fibrocolonoscopic examinations revealed that the false polyps and ulcers on the colonic mucosa had involved the descending colon and the part of the sigmoid colon.The operation time was 170 min and 190 min,respectively,and the intraoperative blood loss was 150 ml and 200 ml,respectively.Pathological examinations after the operation verified the presence of intestinal tuberculosis.No short-term complications occurred after the operation.The frequency of defecation was 5~6 times daily at short-term postoperative period and 1~2 times daily at 5~6 months after the operation.The patients' body weight increased by 2.5 kg and 4 kg,respectively. Conclusions Combined use of laparoscopy and fibrocolonoscopy can accurately evaluate the affected extent of the lesion.Laparoscopic subtotal colectomy is safe and feasible.

Objective To explore the feasibitity of endoscopic thyroidectomy through armpit or breast approach. Methods 14 patients aged from 21 to 36 years.There were 11 cases of thyroid adenoma(9 cases of single side and 2 cases of double sides)and 3 cases of nodular goiter (1 case of single side and 2 cases of double sides).The mini-mcision was made at armpit or breast.A percutaneous chennel into thyroid gland was performed and the focus was removed with ultrasonic scalpel wnder endoscopy. Results All cases were operated on under endoscopy,in cluding 11 cases through armpit approach and 3 ones through breast approach.The mean operative time was 135(105～335)min.The average blood loss was 65ml through armpit approach and 135ml through breast approach respectively.There was no operative complications. Conclusions Thyroidectomy under endoscopy is a new secure method.

Objective To explore the feasibility of laparoscopic splenectomy and cholecysterctomy in the treatment of patients with congenital hemolytic anemia and cholecystolithiasis. Methods 8 patients with congenital hemolytic anemia and cholecystolithiasis were operated on by single-stage splenectomy and cholecysterctomy. Results The procedures were successfully accomplished in all patients. The average hospitalization was 7.5 days. No intraoperative and postoperative complications occurred in all of the 8 patients. Conclusions Single-stage laparoscopic splenectomy and cholecysterctomy is the therapy of choice for patients with congenital hemolytic anemia and cholecystolithiasis. The kind of operation is a minimally invasive approach and has the advantages of less injury and short hospital stay.

Objective To explore the feasibility of laparoscope combined with cholangioscope in the management of bile duct calculus. Methods 100 cases of extra- and/or intrahepatic bile duct underwent laparoscopic choledocholithotomy and T-tube drainage or intra- or postoperative cholangioscope was performed from July 1997 to December 2000. Results 42 out of 100 cases obtained intraoperative complete clearance of the bile duct calculus and other 58 cases achieved thorough clearance of calculus postoperatively once to six times. No residual calculus was found in 3 years of follow-up. Conclusions Laparoscopic and cholangioscopic surgery is an effective method for the treatment of bile duct calculus.

Objective To evaluate the feasibility of endoscopic thyroidectomy via an incision under the armpit. Methods A skin incision was made under the armpit and a channel was established to thyroid gland and endoscopic thyroidectomy was performed in 32 cases suffering from unilateral thyroid lesion including 24 cases of solitary adenoma, 5 cases of multiple adenoma, 3 cases of cystic goiter. Results The average operative time was 125 min and the average blood loss was 55 ml. There were not any postoperative complications. Conclusion Endoscopic thyroidectomy is a mini-invasive surgery. The operative field was clear and ultrasonic scalpel is capable of dealing with blood vessels during the procedure.

BACKGROUND:Surgical management of sternoclavicular joint dislocation takes relatively larger risks for its adjacent mediastinal and other important structures. However, the surgical methods are varied and remain controversial.
OBJECTIVE:To investigate the feasibility and validity of intramedul ary fixation of the proximal clavicle and sternum for sternoclavicular joint dislocation.
METHODS:On 22 adult cadaver upper limb specimens, the anatomical features of the sternoclavicular joints and associated ligaments, the size of articular surface between the medial clavicle and manubrium and the morphology of articular disc, costoclavicular ligament and sternoclavicular ligament were observed and measured. From January 2009 to July 2012, three patients with sternoclavicular joint dislocation received the intramedul ary fixation with wire rope or absorbable suture in figure-of-eight shape. They were males, aged 9, 45 and 62 years, with an average age of 38.6 years. The outcome was evaluated by upper limb function DASH score.
RESULTS AND CONCLUSION:Anatomic results demonstrated that the anteroposterior and coronal diameters of the surface of the medial clavicle were greater than that of the sternum, so they did not match each other. The minimum thickness of the manubrium sterni was at the superior margin of articular surface and the largest was at the sternal angle. The minimum width of the manubrium sterni was at the superior margin of articular surface and the largest was at the inferior margin. The articular disc, liked an el ipse and matched with the articulating surface of the manubrium sterni more, whose anteroposterior diameter was greater than its coronal diameter. Central thickness was greater than the edge thickness. The costoclavicular ligament was thicker and denser than the anterior and posterior sternoclavicular ligament. The anterior and posterior sternoclavicular ligaments were close in length, width and thickness. Clinical trial results demonstrated that al cases were fol owed up for 14 to 36 months. DASH score was 10-16, 13.3 in average. Effects of internal fixation were satisfactory. Surgical management of sternoclavicular joint dislocation by the fixation of the proximal clavicle and sternum through the intramedul ary cavity with wire rope or absorbable suture is reliable and effective.

Objective To evaluate the clinical efficacy of a combination of Ilizarov technique, bone grafting and ankle traction for treatment of nonunion of the distal tibia within 2 cm around the ankle joint. Methods A retrospective study was performed on the patients who had been treated at Department of Orthopaedics, Sir Run Run Shaw Hospital from September 2014 to January 2018 for nonunion of the distal tibia within 2 cm around the ankle joint. They were 8 men and 4 women, aged from 32 to 56 years ( average, 41 years). Their injury was all unilateral, involving 5 left and 7 right ankles. They had all received internal fixation at other hospitals for 6 to 18 months. After we treated them simultaneously with Ilizarov technique, bone grafting and ankle traction, they could walk with crutches as soon as 3 days after surgery in some cases. Their affected limbs were then subjected to gradual weight-bearing walking until they could walk normally without any aid 3 to 6 months after surgery. Their postoperative ankle functions were evaluated according to the ankle-hindfoot scores of the American Orthopedic Foot and Ankle Society ( AOFAS) system. Results The 12 patients were effec-tively followed up for 12 to 24 months ( average, 19 months). All their incisions healed well with no incision or pin-tract infection. All their bone nonunions healed after circular external fixation for 12 to 36 weeks. Their AOFAS scores ranged from 75 to 96 points, showing good functional recovery of the ankle joint. Conclusion A combination of Ilizarov technique, bone grafting and ankle traction is an effective treatment for nonunion of the distal tibia within 2 cm around the ankle joint, leading to preservation of the ankle function and prevention of posttraumatic ankle osteoarthritis.

Vestibular migraine （VM） is a type of paroxysmal vertigo with headaches. The condition is very common in clinical practice，but its pathophysiological mechanism is not fully understood. The clinical manifestations of VM vary greatly and are similar to or overlap with peripheral vertigo diseases such as benign paroxysmal positional vertigo （BPPV） and Meniere's disease （MD），and the neurological examinations of VM usually have no special findings. Therefore，there is no objective gold standard for diagnosis，and exclusive diagnoses are required. Due to the lack of high-level evidence-based studies on the treatment of VM，previous reviews may have been at serious risk of bias due to poor study quality and significant heterogeneity. Despite the poor quality of the overall evidence base for VM treatment，we have systematically performed a clinical practicability-oriented evaluation in this review on the evidence for various treatment options for VM. At the same time，we make treatment recommendations based on this evaluation，hoping to help clinicians in treating patients with VM.

Liver cholesterol metabolism disorder plays an important role in the development of non-alcoholic fatty liver disease (NAFLD).In order to reveal the molecular mechanism of cholesterol homeostasis imbalance induced by saturated fatty acids, HepG2 cells were stimulated with palmitic acid (PA).Lipids accumulation was analyzed by Oil Red O staining, intracellular triglyceride and cholesterol quantification.The level of genes and proteins related to cholesterol homeostasis was measured by RT-qPCR and western blotting.Additionally, intracellular bile acids and mitochondrial oxysterols were detected by LC-MS/MS.The results demonstrated that intracellular lipids such as TG and TC were significantly increased in the model with PA stimulation.Although no significant difference was detected in genes related to cholesterol synthesis and uptake, the protein expression of ABCG5 and LXRα were significantly down-regulated, indicating a decrease in cholesterol efflux.Meanwhile, the gene expression of STARD1 and CYP7B1, which are responsible for bile acid alternative synthesis, were markedly enhanced, along with a significant increase of cholesterol and 27-OHC in mitochondria and CDCA in cells.These results suggested that PA overload may disrupt cholesterol homeostasis by inhibiting cholesterol efflux and promoting bile acids synthesis.