The histological lesions of toxic hepatitis of wistar rat induced by carbon tetrachloride (CCl_4) were observed with electron microscope. We found that in sinusoids or inflamatory infiltrated areas. there were classical matured plasma cells and 4 typesof plasmablast cells with characteris tic morphological criteria. cccrrrsss,,,According to the direct injuring effect of CCl_4 on liver, we deduced that the humoral immunological reaction was the result of toxic hepatitis and not its cause.

Hepatic fibrsosis was induced by carbon tetrachloride in mice C 57.Then the feed containing Dangshen pollen was given to the animals. It was demonstrated under the electron microscope that the Dangshen pollen lowered the activity of the fibroblasts around the central vein and the fatstoring ceils of sinusoids, and lowered the grade of collagenous fibers of Disse's spaces (P

It was observed under an electron microscope that in the hepatic piecemeal necrotic area of CAH patients,there were T lymphocytes,plasma cells,monocytes,fatstoring cells and proliferating Hering's tubules.The more severe the patient's condition was,the much more the sorts and numbers of these cells were.We consider that the above observations by electron microscopy afford valuable morphological information about the severity of CAH.

It has been repored that the Biopsy positive rate of HBsAg in the liver specimens of the patients of hepatitis B was only 70% or so.We have observed with electron miroscopy the liv- er biopsy tissues and found that its positive rate was as high as 93.8%.Under electron microscopy,we could identify the HBsAg filaments as tubular,branching-tubular and tubu- lar-vesicular.We have also found that it was not easy to find the HBsAg filaments in the pa- tients of CAH(only 9% was found)and it was easy to be found(78.9%)in the patients of CPH or CLH. It had a high significant difference in the two groups.

Specimens from 12 cases of pancreatic endocrine tumors were investigated by light and electron microscopy and immunohistochemical staining. Pancreatic stem cell-like cells with special characteristics of pancreatic stem cell were found in the proliferating small ductules and dispersed at the margins of pancreatic endocrine tumors in all the cases studied.

By electron microscopic observation,we found few pit cells in liver,spleen.lungs,and small intestine in Wistar rat.The pit cells show several morphologicalcharacteristics as follows:1.electron dense granules in cytoplasm,which aresurrounded by a limiting membrane;2.vesicles with rod shaped bodies incytoplasm;3.eccentric nucleus with marginal heterochromatin;4.well developedpseudopodia.In addition,the pit cells have also been found in breast carcinoma in3 patients.According to their distribution and morphology,we preliminarilydeduced they probably belong to natural killer cells and may act as a cytotoxicagent and an immunological defender against carcinoma.

【Objective】 To establish a method for detecting human immunoglobulin Fc function based on surface plasmon resonance technology. 【Methods】 Based on the characteristic that FcγRI can be binded to the Fc segment of IgG, the affinity constant of the sample was detected by surface plasmon resonance, and its Fc function was the KD ratio of the sample to the standard. The method was validated for specificity/specificity, precision and robustness. The method and the pharmacopoeia method were used to detect the Fc function of 30 human immunoglobulins, and the correlation and consistency of the detection results were analyzed. 【Results】 The method validation results showed that this method has strong specificity/specificity (t values were 0.15, 0.22, both P>0.05), good precision (CV value 5.37%~10.69%) and good robustness (CV value 10.06%). The detection results of this method and the pharmacopoeia method have high correlation (r=0.96, P<0.05) and high consistency (Bias-2.060, 95% Limits of Agreement-5.628~1.508). 【Conclusion】 A method for detecting human immunoglobulin Fc function based on surface plasmon resonance has been successfully established.

Based on randomized block design of experiment, agronomic traits and yields of 14 F1 generations of Dendrobium officinale were determined. The results showed that the differences in agronomic traits and yields among families were significant, and the hybrid vigor was obvious. Families of 6b x 2a, 9 x 66 and 78 x 69 were selected with the remarkable superiority of yields, agronomic traits and product customization. Correlation analysis between agronomic traits and yields showed that plant height, stem diameter, leaf number, blade length and blade width were all significantly correlated with biological yields and economic yields. Among which, stem diameter, leaf number and blade length were the most significant, and an optimal linear regression model could be established. When the number of shoots was fewer than 4.5, both biological yields and economic yields increased with the increasing number of shoots, but it could not much affect yields when the number of shoots was larger than 4.5. Shoots number, stem diameter and leaf index were basic stability when compared biennial traits to annual, which could be used for early selection.
Biomass ; Dendrobium ; genetics ; growth & development ; Hybridization, Genetic ; Plant Leaves ; genetics ; growth & development ; Plant Stems ; genetics ; growth & development ; Quantitative Trait Loci

Using phenol-sulfuric acid method and hot-dip method of alcohol-soluble extracts, the contents of polysaccharides and alcohol-soluble extracts in 11 F1 generations of Dendrobium officinale were determined. The results showed that the polysaccharides contents in samples collected in May and February were 32.89%-43.07% and 25.77%-35.25%, respectively, while the extracts contents were 2.81%-4.85% and 7.90%-17.40%, respectively. They were significantly different among families. The content of polysaccharides in offspring could be significantly improved by hybridization between parents with low and high polysaccharides contents, and the hybrid vigor was obvious. Cross breeding was an effective way for breeding new varieties with higher polysaccharides contents. Harvest time would significantly affect the contents of polysaccharides and alcohol-soluble extracts. The contents of polysaccharides in families collected in May were higher than those of polysaccharides in families collected in February, but the extracts content had the opposite variation. The extents of quantitative variation of polysaccharides and alcohol-soluble extracts were different among families, and each family had its own rules. It would be significant in giving full play to their role as the excellent varieties and increasing effectiveness by studying on the quantitative accumulation regularity of polysaccharides and alcohol-soluble extracts in superior families (varieties) of D. officinale to determine the best harvesting time.
Breeding ; China ; Dendrobium ; chemistry ; classification ; genetics ; Plant Extracts ; chemistry ; isolation & purification ; Polysaccharides ; chemistry ; isolation & purification