[Objective]To observe the effect and mechanism of cortex phellodendron on uric acid nephropathy.[Methods]32 male SD rats were divided into four groups including control group,model group,cortex phellodendron treated group and allopurinol treated group.Uric acidnephropathy model rats were established by using yeast and adenine,then treated with Sodium Chloride,cortex phellodendron or allopurinol respectively for 30 days.Blood urea nitrogen(BUN),creatinine(Cr),uric acid(UA)in blood were examined,and the expressions of tumor necrosis factor alpha(TNF-?)and cyclooxygenase 2(COX-2)in renal tissue were detected by immunohistochemistry technique.[Results]Compared with model group,the levels of BUN,Cr,UA in blood and the expression of COX-2 and TNF-? in renal tissue in cortex phellodendron treated group decreased significantly.[Conclusion]Cortex phellodendron has a beneficial effect on uric acid nephropathy in rats.It could decrease the expression of TNF-? and COX-2 in renal tissue of uric acid nephropathy rats so as to alleviate the damage of kidney and protect renal function.

Kushenhuangqi Injection is made from Chinese herb medicine flavescent sophora and astragalns roots.Seventy-one patients with malignant pleural effusion were randomly divided into two groups: 36 patients were treated with both intrathoracic Kushenhuangqi Injection and chemotherapeutic agent cisplatin (treatment group) and 35 patients were treated with cisplatin alone.The response rate of treatment group was significantly higher than that of control group (31/36 vs 20/35,u=3.020,P<0.01).The rate of adverse reaction (leucopenia,nausea,vomiting etc.) in treatment group was significantly lower that that of control group.The results indicate that Kushenhuangqi Injection can enhance the therapeutic effect of chemotherapeutic agent and reduce its adverse reaction in treatment 0f malignant pleural effusion caused by carcinoma of the lungs.

Long non-coding RNAs (lncRNA) play important roles in many biological processes.However,compared with other types of RNAs,limited research has been conducted on lncRNA,mostly concerning its relationship with tumor,and there are much fewer studies on its relationship with atherosclerosis.In this article,we summarized the latest research findings in this field,with an emphasis on the importance of IncRNA in atherosclerosis.The value of lncRNA in targeted therapy of atherosclerotic disorders is also discussed.

Objective To investigate the expression of TGF-?1 and Smad 3, 7 in rats renal tissue after unilateral ureteral obstruction(UUO) and the effect of Shentong capsule on their expressions. Methods Sixteen rats underwent UUO and were devided into operation group and Shentong treatment group, additional 8 rats were shame operation group. The pathological change of renal tissues were examined by light microscopy 8 days after operation. The extent of renal fibrosis was determined by measuring the area of renal interstitial fibrosis. Immunhistochemistry was applied to detect the expression of TGF-?1 and Smad 3,7. Results In comparison with shame operation group, the expression of TGF-?1 and Smad 3 in operation group significantly increased, and the expression of Smad 7 significantly decreased(P

Objective To explore the value of combining pulmonary rehabilitation with heat and magnetic vibration (HMV) therapy and tiotropium for patients with stable but severe chronic obstructive pulmonary disease (COPD). Methods This was a paralleled, controlled, randomized study. Thirty-seven patients with stable severe COPD were enrolled and divided into two groups at random. One was the tiotropium group (T group) , while the other combined tiotropium therapy with HMV (the T + HMV group). The time span was 4 weeks. The examinations were performed at week 0, week 2 and week 4. The examinations included pulmonary function tests, arterial blood gas analysis, the 6 minute walking test (6MWT) , Borg's score and St George's Respiratory Questionaire (SGRQ). Results Inspiratory capacity (IC) increased in both groups. Forced expiratory volume in one second ( FEV1.0) , percent predicted FEV1.0 and FEV1.0/forced vital capacity ( FVC) increased significantly only in the T + HMV group. The average parameters of the pulmonary function test in the T + HMV group were significantly higher than in the T group. In both groups, alveolar PO2 ( PaO2) improved but alveolar PCO2 ( PaCO2 ) did not change and in this there was no significant difference between the groups. The 6 minute walking distance increased and the average Borg score decreased in both groups, and there was no difference between the groups. SCRQ dropped more than 4 scores in both groups, but the decrease in the T + HMV group was significantly greater. Conclusions Tiotropium can play an important role in the rehabilitation of patients with stable severe COPD. The combination of tiotropium with HMV therapy is superior to tiotropium alone in pulmonary rehabilitation for stable but severe COPD patients.

Objective To investigate the role of nuclear factor-kappa B(NF-?B) and monocyte chemoattractant protein-1(MCP-1) in the pathogenesis of uric acid nephropathy.Methods Twenty male SD rats were divided into control group(group C) and model group(group M).The model rats with uric acid nephropathy were made using adenine.Immunohistochemistry was used to detect the expressions of NF-?B and MCP-1 in 18 days and the serum uric acid and renal fuction were measured respectively.Results In comparison with group C,the expressions of NF-?B and MCP-1 in the rats of group M were significantly increased(all P

Objective To evaluate the standardized drug treatment and its influence factors of patients,with coronary artery disease,in hospital and one year after discharge.Methods The study enrolled sequentially 165 patients who were firstly diagnosed of coronary artery disease,61 cases with stable angina,67 cases with unstable angi-na and 37 cases of acute myocardial infarction,by coronary artery angiography from 2010 to 2012.The standardized drug treatment and its influence factors of patients were analyzed at hospital and 1 year after discharge in the present study.Results Fifty five percent patients with coronary artery disease at hospital regularly took the four drugs,anti-platelet agents,statins,beta blockers and angiotensin converting enzyme inhibitors/angiotensin II receptor antagonist. The ratio decreased to forty five percent one year after discharge(χ2 =81.04,P <0.01).The reasons of the irregular medication taken were optional withdrawal(61%),following the doctors′advice(15%),economic hardship(20%) and the drugs′adverse reaction(4%).Conclusion The results of the present study showed that the rate of regular medication in patients with coronary artery disease is low in hospital and 1 year after discharge.The major reasons of the irregular medicine taken were the patients′optional withdrawal and the consciousness of second prevention was lack in doctors in our hospital.Therefore,the increase of the consciousness of regular standardized treatment in prima-ry care physicians and strengthen the management of the patients after discharge form hospital.

Objective : To explore the effects of different implant sites combined with pterygomaxillary implantation on the biomechanics of implant and bone tissue，and to provide a basis for clinical selection of implant design in accordance with biomechanical principles． Methods: The cone-beam CT scan data of an edentulous maxillary with inadequate posterior bone were selected to complete the establishment of the three-dimensional entity model of the maxilla．The prosthesis and implant-abutment integrated three-dimensional entity models were established using the prosthesis and implant data ，and five groups of three-dimensional finite element models of different implant sites were designed．A unilateral vertical load of 200 N and an oblique load of 100 N were applied to the bilateral posterior dental area，respectively．ANSYS finite element analysis software was used to calculate the stress distribution on the surface of the implant and surrounding bone tissue．SPSS 26. 0 software package was used to statistically analyze the data. Results : ① All the five models showed that the maximum stress was concentrated in the neck of the implants and cortical bone. ② The maximum stress value of the implant under oblique loading was greater than that under vertical loading (P＜0. 05) ，but there was no significant difference in the maximum stress value around bone tissue in the 5 groups of models. ③ There was no significant difference in the maximum stress of the implant and bone between the different implant sites combined with pterygomaxillary implants． Conclusion In the fixed restoration of maxillary edentulous implants，pterygomaxillary implants implanted symmetrically on both sides and changing the position point of the anterior implant do not affected the stress distribution of the whole design．In clinical practice，suitable sites can be selected according to the residual bone mass of patients，and combined with pterygomaxillary implantation for implant design.

Objective: To explore the effects of dendritic epidermal T cells (DETC) on proliferation and apoptosis of epidermal cells in wound margin of mice and its effects on wound healing. Methods: Twenty-eight healthy specific pathogen free (SPF) C57BL/6 wild-type (WT) male mice aged 8-12 weeks and 60 SPF T lymphocyte receptor δ-knockout (TCR δ^-/-) male mice aged 8-12 weeks were selected to conduct the following experiments. (1) Eight WT mice were selected to isolate epidermal cells and primarily culture DETC according to the random number table. Morphological observation and purity identification of DETC by flow cytometer were detected immediately after culture and on culture day (CD) 15 and 30, respectively. (2) According to the random number table, 5 WT mice and 5 TCR δ^-/- mice were selected and enrolled into WT control group and TCR δ^-/- group. Round full-thickness skin defect with diameter of 6 mm was made on the back of each mouse. The wound healing condition was observed immediately after injury and on post injury day (PID) 2, 4, 6, 8, 10, and the percentage of residual wound area was calculated. (3) Mice were selected to group and reproduce model of full-thickness skin defect as in experiment (2). On PID 3, the tissue of wound margin was collected for hematoxylin eosin staining, and the length of new epithelium was measured. (4) Mice were selected to group and reproduce model of full-thickness skin defect as in experiment (2). On PID 3, epidermal tissue of wound margin was collected to determine expression of proliferating cell nuclear antigen (PCNA) using Western blotting for evaluation of proliferation of epidermal cell. (5) Mice were selected to group and reproduce model of full-thickness skin defect as in experiment (2). On PID 3, epidermal tissue of wound margin was selected and digested into single-cell suspension, and apoptosis of cells was detected by flow cytometer. (6) Forty TCR δ^-/- mice were selected to carry out the same treatment as in experiments (2)-(5). According to the random number table, these mice were enrolled into TCR δ^-/- control group and TCR δ^-/-+ DETC group, with 5 mice in each group for each experiment. Round full-thickness skin defect was made on the back of each mouse. DETC in the number of 1×10⁵ (dissolution in 100 μL phosphate with buffer purity above 90%) were injected through multiple points of wound margin of mice in TCR δ^-/-+ DETC group immediately after injury, and equal volume of phosphate buffer was injected into mice of TCR δ^-/- control group with the same method as above. Data were processed with one-way analysis of variance for repeated measurement, t test, and Bonferroni correction. Results: (1) Along with the culture time elapse, the number of dendritic structures of DETC increased gradually. The percentage of T lymphocytes was 4.67% and 94.1% of these T lymphocytes were DETC. The purity of DETC on CD 15 was 18.50% and the purity of DETC on CD 30 was 98.70%. (2) Immediately after injury, the wound healing condition of mice in WT control group and TCR δ^-/- group was similar. The wound healing speed of mice in TCR δ^-/- group was slower than that in WT control group on PID 2-10. The percentages of residual wound area of mice in TCR δ^-/- group on PID 2, 4, 6, 8, and 10 were increased significantly compared with those in WT control group (t=3.492, 4.425, 4.170, 4.780, 7.318, P<0.01). (3) The length of new epithelium of mice in TCR δ^-/- group on PID 3 was (359 ± 15) μm, which was obviously shorter than that in WT control group [(462±26) μm, t=3.462, P<0.01]. (4) Immediately after injury, wound condition of mice in TCR δ^-/-+ DETC group and TCR δ^-/- control group was similar. Compared with TCR δ^-/-+ DETC group, the wound healing speed of mice in TCR δ^-/- control group were obviously slower on PID 2-10. The percentages of residual wound area of mice in TCR δ^-/-+ DETC group on PID 2, 4, 6, 8, and 10 were decreased significantly compared with those in TCR δ^-/- control group (t=2.308, 3.725, 2.698, 3.707, 6.093, P<0.05 or P<0.01). (5) On PID 3, the length of new epithelium of mice in TCR δ^-/-+ DETC group was (465±31) μm, which was obviously longer than that in TCR δ^-/- control group [(375±21) μm, t=2.390, P<0.05]. (6) On PID 3, PCNA expression of epidermal cell in wound margin of mice in TCR δ^-/- group was 1.25±0.04, which was obviously lower than that in WT control group (2.01±0.09, t=7.415, P<0.01). (7) On PID 3, PCNA expression of epidermal cell in wound margin of mice in TCR δ^-/-+ DETC group was 1.62±0.08, which was significantly higher than that in TCR δ^-/- control group (1.05±0.14, t=3.561, P<0.05). (8) On PID 3, apoptosis rate of epidermal cell in wound margin of mice in TCR δ^-/- group was (16.1±1.4)%, which was higher than that in WT control group [(8.1±0.6)%, t=5.363, P<0.01]. (9) On PID 3, apoptosis rate of epidermal cell in wound margin of mice in TCR δ^-/-+ DETC group was (11.4±1.0)%, which was obviously lower than that in TCR δ^-/- control group [(15.4±1.4)%, t=2.377, P<0.05]. Conclusions DETC participates in the process of wound healing though promoting the proliferation of epidermal cells in wound margin and inhibit the apoptosis of these cells.