Objective To explore the application of combined penile and scrotum skin flap in the reoperation process for operation failure cases with hypospadias.Methods A retrospective analysis was performed with the inclu-sion of 1 5 failure cases with hypospadias who received combined penile and scrotal skin flap treatment for reoperation from January 201 3 to December 201 5 in the First Affiliated Hospital of Guangxi Medical University.These cases aged from 7 to 1 5 years old,with a median age of 1 0.2 years old.Among them,a 3 rd operation was performed in 4 cases,4th operation in 5 cases,5 th operation in 4 cases,and 6 th operation in 2 cases.The procedure of Onlay island flap urethro-plasty +tubularized incised plate (Onlay +TIP)urethroplasty was performed in 4 patients,and the other 1 1 cases were treated with urethral mouth basal vascular skin tube urethroplasty (Mathieu)+TIP urethroplasty.F8 or F1 0 silicone balloon catheter were used as stent in new urethra,outer dressing after operation was replaced every 2 to 3 d,the outer dressing was demolished 6 -8 d after operation.Results All patients were followed up after surgery until March 20, 201 6,the follow -up time was 4 -26 months.In these 1 5 patients,the success rate of operation was 86.7%(1 3 /1 5 ca-ses),among them,3 cases were success and 1 case was failed in Onlay +TIP group,and 1 0 cases were success and 1 case was failed in Mathieu +TIP group.The patients with successful operation were with beautiful appearance of the pe-nis,normal position of the meatus urinarius and normal urination.No peristome of urethra,urethrovaginal fistula or ure-thral diverticulum was found after surgery.However,urethral cracking of the glans of penis appeared in 2 operation failed patients,and the orificium urethrae externum was low -set,and urethroplasty was needed 1 year postoperatively. Conclusions For some failure cases with hypospadias showing suitable anatomy conditions,Onlay +TIP or Mathieu +TIP combined with penile and scrotal flap may be a good choice for the completion of reoperation.

Objective To discuss the influence of drug compatibility on the components of the volatile oil in Flos Lonicerae and Fructus Forsythiae. Methods GC-MS system was used to analyze the volatile oil in Flos Lonicerae, Fructas Forsythiae and their combination. The effect of compatibility on the components and content of volatile oil was evaluated. Results The results of GC-MS showed that gerani]o, tetradecanoic acid methyl ester, hexadecanoic acid ethyl ester, fluoranthene, 9, 12, 15-Octadeeatrienoic acid ethyl ester and linoleic acid was detectable in Flos Lonicerae but was undetectable in the drug pair; camphene, alpha-terpinene, perillaalcohol, 2-beta-pinene, beta-hexadecanal, phellandrene was de-tectable in Fructus Forsythiae but was undetectable in the drug pair; limonene, isolongifolene, nonadecane was detectable in the drug pair but was undetectable in the two single drugs. Conclusion The compatibility of the drug pair has an effect on components and contents in the volatile oil of Flos Lonicerae and Fructus Forsythiae.

BACKGROUND:Non-heart beating donors have become the most promising source of donor in recent years. Tofurther improve donor preservation solution, on the basis of conventional storage solution hypertonic citrate adenine solution, artificial cordyceps was added to prepare artificial cordyceps compound solution. It is hoped to reduce donor injury and to elevate donor quality by antioxidation. OBJECTIVE: To investigate the preservation effect of artificial cordyceps compound solution on isolated renal from non-heart-beating rats. METHODS: Medulas of 39 Sprague-Dawley rats were injured. Withoutin vitro respiratory and circulatory supports, non-heart-beating models were established and randomly divided into three groups: artificial cordyceps compound group (n=15), hypertonic citrate adenine solution group (n=15) and physiological saline group (n=9). The kidneys of rats in different groups were stored in artificial cordyceps compound solution, hypertonic citrate adenine solution and physiological saline at 4℃. RESULTS AND CONCLUSION: Compared with the hypertonic citrate adenine solution group, after 6 and 12 hours of preservation, morphological injury was mild, apoptotic index, malondialdehyde, Bax and Caspase-3 expression levels decreased, superoxide dismutase activity and Bcl-2 expression increased; after 24 hours, the degree of injury was similar, malondialdehyde content decreased, and superoxide dismutase activity increased in the artificial cordyceps compound group. These findings indicated that the preservation effect of artificial cordyceps compound on antioxidation and inhibition of apoptosis was better than that of hypertonic citrate adenine solution in non-hearting beating rats.

Aims Tocomparethepharmacokineticsof ephedra alkaloids,cinnamic acid and cinnamic alcohol in Ephedrae, Cinnamomi and Ephedrae-Cinnamomi herb couple through UPLC-MS/MS in rats respective-ly,and to investigate the effect of combination on phys-iologicaldisposition.Methods Plasmasampleswere collected at different times after oral administration of Ephedrae,Cinnamomi and Ephedrae-Cinnamomi herb couple extracts.The concentrations of ephedra alka-loids,cinnamic acid and cinnamic alcohol in plasma samples were determined by UPLC-MS/MS.DAS 3. 0 was used to calculate pharmacokinetic parameters.The differences of samples in two groups were conducted with univariate statistical analysis using SPSS 13. 0. Results ComparedwithEphedraegroup,theCmaxof norephedrine hydrochloride, norpseudoephedrine hydrochloride,ephedrine hydrochloride,pseudoephed-rine hydrochloride,and methylephedrine hydrochloride in Ephedrae-Cinnamomi herb couple group were signif-icantly greater (P<0. 05 );the AUC0-t of norpseudo-ephedrine hydrochloride was significantly greater (P<0. 05 );the MRT0-t of norephedrine hydrochloride, phedrine hydrochloride, pseudoephedrine hydrochlo-ride,and methylephedrine hydrochloride were signifi-cantly less (P <0. 05 );the T1/2z of norephedrine hydrochloride,phedrine hydrochloride,and methyl-ephedrine hydrochloride were significantly less (P <0. 05 ).The AUC0-t and MRT0-t of cinnamic acid and cinnamic alcohol were significantly greater than those inCinnamomigroup(P<0.05).Conclusion The combination of Ephedrae and Cinnamomi improves the absorption concentration of five ephedra alkaloids, Slows down the elimination of norephedrine hydrochlo-ride,phedrine hydrochloride,pseudoephedrine hydro-chloride,and methylephedrine hydrochloride,and in-creases the bioavailability of cinnamic acid and cin-namic alcohol.

Objective To evaluate the pharmacological effect of Zhixie Tuire Tablets(ZTT) on human rotavirus RV709 in host cells in vitro.Methods By improved methods of methyl thiazolyl tetrazolium colorimetry assay(MTT) and cytopathic effect(CPE),the anti-rotavirus effect of ZTT on RV709 was evaluated.Results The half toxic concentration(TC50) of ZTT for MA104 was 0.49699 mg/mL.ZTT neither can directly kill human rotavirus,nor prevent human rotavirus from invasion of host cells.The halft inhibitory concentration of ZTT was 0.00519 mg/mL for RV709.Conclusion In-vitro anti-rotavirus mechanism of ZTT is related to the inhibition of the biological synthesis of rotavirus in host cells.

Objective To probe into the therapeutic mechanism of Zhixie Tuire Tablet (ZTT) for rotavirus-induced diarrhea. Methods We established the 4-day old Kunming neonatal mice model by infecting group A G3 type of rotavirus,and then gave the inoculated mice with ZTT at the dose of 0.78 g/kg for three days. Fluorescent quantification PCR method was used to detect the changes of sodium glucose transporter SGLT1 mRNA level and radio-immunity method was used for the examination of PGE2 level in neonatal mice small intestine. Results SGLT1mRNA level was lower in ZTT group than that in the normal group,the difference being significant (P 0.05). PGE2 level in ZTT group was higher than that in the normal group,but lower than that in the model group (P

Object To develop the quantitative method of cinnamaldehyde in Mahuang Decoction (MHD) by GC-MS and study the influence of various combinations of MHD on the contents of cinnamaldehyde in the decoctions. Methods By L_8(2~7) orthogonal design and statistic analysis (SPSS 10.0), eight decoctions with Ramulus Cinnamomi were prepared and analyzed. Contents of cinnamaldehyde were determined by GC-MS. The pH value of decoctions was determined by pH meter. Results For Herba Ephedrae and Semen Armeniacae Amarum, the influences on the contents of cinnamaldehyde were significant (P0.05). The change of pH value not obvious. Conclusion The content of cinnamaldehyde is only reduced significantly by Herba Ephedrae and Semen Armeniacae Amarum. But it is insignificant for Radix Glycyrrhizae and its combinations.

Objective To develop a gas chromatography-mass spectrometry (GC-MS) method for the determination of ephedrine (E) and pseudoephdrine (PE) and to study the pharmacokinetics features of E and PE in healthy human volunteers. Methods The serum concentrations of E and PE were measured by GC-MS. The pharmacokinetic parameters were computed by using WinNonlin program. Results A good linearity was obtained in E from 5 ng/mL to 1000 ng/mL (r2=0.9985) and in PE from 2.5 ng/mL to 500 ng/mL (r2=0.9994). The recoveries were in the range of 107.5 %to 96.2 %and 102.5 %to 93.9 %for E and PE respectively. The interday RSD and intraday RSD of E and PE were both less than 5 %. The E and PE plasma solution were steady within 60 days. Conclusion This GC-MS method is a accurate and reliable method for the determination of E and PE in human plasma.

Object To establish a quantitative method for ephedrine and pseudoephedrine in the aqueous decoctions of MAHUANG TANG (ADMHT) by GC MS, and to determine the content of the two components in decoctions of the different recipes consisted of Herba Ephedrae, and other herbs by using the quantitative method will be established, so as to study the effect of compatible medicinal herbs on the release of the two components in Herba Ephedrae to decoctions Methods The quantitative method by the help of characteristic ion was used in GC MS Results The content of ephedrine and pseudoephedrine in decoction prepared from Herba Ephedrae and Semen Armeniacae Amarum evidently decreases, the content of two components in decoction prepared from Herba Ephedrae and Ramulus Cinnamomin evidently increases, the content of two components in decoction prepared from Herba Ephedrae and Radix Glycyrrhizae has no obvious change, as compared with that of two components in decoction from Herba Ephedrae alone Conclusion The method is good in the aspect of separable effect, sensitivity and reproducibility The method not only can quantify the two components, but also provide the bases of quality control for the pharmacokinetic study and therapeutic detection of Herba Ephedra recipes

Objective To investigate the changes of cortical thickness and surface area in patients with bipolar depression(BD),and to explore the relationship between abnormal changes in gray matter and clinical symptoms.Methods 28 BD patients and 28 healthy controls underwent T1-weighted MRI.The Freesurfer software was used to process the T1 images,which used a set of automated sequences to analyze cortical thickness and surface area on 66 regions (33 regions of each hemisphere),and the correlation with clinical features was also calculated.Results Compared with controls,BD patients showed thinner cortical thickness in left medial orbitofrontal cortex((2.40±0.12) mm vs (2.55 ±0.18) mm,P=1.2× 10-3) and left rostral anterior cingulate((2.66±0.21) mm vs (2.88±0.27) mm,P=3.1 × 10-4),and smaller area of left cuneus((1 443.13± 131.00) mm2vs (6 634.70±600.16) mm2,P=2.7× 10-4) and right superior frontal gyrus ((6 634.70±600.16) mm2vs (7 300.50±653.39) mm2,P=1.3× 10-3).In addition,the negatively correlation was found between the cortical area of left cuneus and effective illness duration (r=-0.471,P=0.018),and the cortical thickness in left rostral anterior cingulate and total score of HAMD-17(17-item Hamilton Rating Scale for Depression) (r=-0.508,P=0.009).Conclusion There are abnormal altertion of cortical thickness and cortical areas of emotional circuit in bipolar depression,but the brain areas are not completely overlapping.Correlation analysis suggests that cortical thickness and area is related to different clinical features.