Doctor-patient trust is the core and foundation of harmonious doctor-patient relationship, because our country is at the stage of social transformation and deepening medical reform, various problems make the doctor-patient relationship unoptimistic, and there appeared crisis of confidence between doctors and patients. This arti-cle analyzes the current situation of doctor -patient trust in our country, based on the clarification of doctor -patient trust. It also comb the researches focused on doctor-patient trust evaluation on the basis of domestic and foreign literature. It is expected to provide a reference to the future evaluation of doctor-patient trust, to improve the level of trust between doctors and patients, and to build a harmonious doctor-patient relationship.

Objective To reevaluate Shanghai Criteria of liver transplantation (LT) for hepatocellular carcinoma (HCC).Method We evaluated the outcome of 433 consecutive patients with HCC who underwent LT from 2001 to 2013 at Zhong shan Hospital,Fudan University.The Kaplan Meier product-limit method with log rank test was used to evaluate survival rate according to different Criteria.Result The 5-year overall survival (OS) rate and relapse-free survival (RFS) rate were as follows:Milan Criteria (n =169; 72.5％ and 79.0％),UCSF Criteria (n =223; 68.2％ and 75.7％) ;Shanghai Criteria (n =255; 65.9％ and 70.6％) ; Pittsburgh Criteria (n =419,55.6％ and 61.2％).There was no significant difference in the 5 year OS and RFS between Shanghai Criteria group and Milan Criteria group (P＞0.05).Conclusion Shanghai Criteria,which modestly expanded current criteria while still preserving similar long term survival,could be more fit for the situation of China.

Precision medicine is currently being paid much attention and greatly promotes the diagnosis and treatment of various diseases,especially cancer.Surgery plays a pivotal role in the treatment of hepatocellular carcinoma (HCC) and precision hepatectomy stands as the most updated surgical idea.Application of the concept and techniques of precision medicine to further improve HCC surgery,consummate the skills and modalities of HCC surgical treatment,enrich the nature of precision hepatectomy,so as to make precise evaluation,early prediction,reduce postoperative recurrence and metastasis,find biomarkers of molecular targeted therapy and improve the prognosis is the prime task of HCC surgery.

0.05).Lymph node metas- tasis,macroscopic vascular invasion,TNM classification,Edmondson pathologic classification,tumor location and capsule,carbohydrate antigen 19-9 (CA19-9) might affect the outcome post-LT.Conclu- sion The overall survival of LT for CCC was dismal.We should select the recipients very carefully and strictly.Those CCC patients with large tumor,TNM class-Ⅲ,bi-lobe distribution or who can not be ruled out lymph node or vascular invasion,should be contraindicated for LT.

Manganese peroxidase (MnP), a crucial enzyme in lignin degradation, has wide potential applications in environmental protection. However, large-scale industrial application of this enzyme is limited due to several factors primarily related to cost and availability. Special attention has been paid to the production of MnP from inexpensive sources, such as lignocellulosic residues, using solid-state fermentation (SSF) systems. In the present study, a suitable SSF medium for the production of MnP by Schizophyllum sp. F17 from agro-industrial residues has been optimized. The mixed solid medium, comprising pine sawdust, rice straw, and soybean powder at a ratio of 0.52:0.15:0.33, conferred a maximum enzyme activity of 11.18 U/g on the sixth day of SSF. The results show that the use of wastes such as pine sawdust and rice straw makes the enzyme production more economical as well as helps solve environmental problems.
Culture Media ; Fermentation ; Industrial Microbiology ; methods ; Oryza ; Peroxidases ; biosynthesis ; Schizophyllum ; enzymology ; Wood

Animals ; Dose-Response Relationship, Drug ; Hydroxylamine ; toxicity ; Male ; Rats ; Rats, Wistar ; Spermatozoa ; drug effects ; Testis ; cytology ; drug effects

Humans ; Nanoparticles ; toxicity ; Skin ; drug effects ; Skin Absorption

Objective To investigate the anti-apoptotic mechanism of tanshinone ⅡA and the function of prohibitin(PHB)onmyocardial cells apoptosis induced by hydrogen peroxide(H2O2).Methods Myocardial cells were primary culturedneonate rat were cultured in medium with 200 μmol/L H2O2,and the medium was supplemented with tanshinone ⅡA(1 × 10-4 mol/L)in advance for 24 h.PHB in myocardial cells was knocked down by RNA interference,and theexpression level of PHB was determined by Western blotting analysis.Flow cytometric analysis was used to detectapoptosis rate,intracellular calcium concentration([Ca2+]i),and mitochondrial membrane potential(MMP).ResultsH2O2-mediated cell apoptosis resulted in activation of PHB,increasing of[Ca2+]i,and decreasing of MMP.TanshinoneⅡA profoundly inhibited myocardial cell apoptosis induced by H2O2,decreased[Ca2+]i,and increased MMP.Specificsilence of PHB by siRNA down-regulated the expression level of PHB,increased apoptosis rate and[Ca2+]i,and decreasedMMP.Conclusion The results demonstrate that tanshinone ⅡA could attenuate apoptosis induced by H2O2,and theactivation of PHB induced by H2O2 is the major regulatory pathway of cyto-protective gene expression against oxidativestress.

0.05). Conclusion Comparedwith DSA and operation,there is excellent correlation among 3D contrast-enhanced moving-bed MRA,DSA and operation.MRA canaccurately and entirely assess occlusive disease of the arteries of the lower extremity.It is a ono-invasive,reliable and potential newtechnique.

Islet transplantation is an effective method for diabetic therapy. This article reviews the recent achievments in the study of induction differentiation of embryonic and adult stem cells into pancreatic islets, and expects that the breakthrough in this field would provide a new method for diabetic therapy.