Objective:To make use of the characteristics of presenting and processing tumor antigen of dendritic cell to enhance generation of isotypical but xenogenous T lymphocyte.Methods:Autologous dendritic cells were obtained by culturing peripheral blood monocyte of patients with cervical cancer in the presence of granulocyte-macrophage colony stimulating factor(GM-CSF) and interleukin-4(IL-4).Dendritic cells were loaded with tumor cell lysate and co-cultured with heterogenous PBMCs from patients to induce generation of tumor specific cytotoxic T cell.The generation rate was measured by MTT.Results:Tumor lysate-loaded DC which was isolated from peripheral blood monocyte of patients with cervical cancer could induce the generation of isotypical but xenogenous T lymphcyte.There was no significant difference between tumor tissue lysate and tumor cell strain lysate(P

objective: To examine the potential of ANP63 and HPV16 E7 as one of biomarkers for cervical premalignant lesions in Thi-nPrep smears, and to search for the identification of specific biomarkers for cervical intraepithelial neoplasia (CIN) as a new examining method to evaluate evolution of CIN. Method; Streptavidin peroxidase (SP) method was used. Immunocytochemical analysis of ANP63 expression was performed in 71 ThinPrep smears. RT - PCR was used to detect the mRNA of HPV16 E7 in 71 ThinPrep liquid - based smears specimens. Result; Positive expression rate of ANP63 in CIN was higher than that in inflammation (P

Objective To study the infection rate of of cytomegalovirus in donor of Qinghai province. The anti-CMV-IgG of 4709 donors were detected by ELISA.Methods The CMV-IgG were detected by ELISA.Results The infection rate of CMV in Qinghai province was 57.7% .This meanings the anti CMV antibody was positive in 2614 donors.The positive rate in femaie(60.7%) was higher than that in male(53.3%).Among diffent race of people,the lowest positive rate was is Tu nationaiity(49.8%) and the highst positive rate was is monglia(64.8%).The CMV infection rate in Qinghai province was nearly to the in Europe(40%~60%)but lower than that in other provinces in our country(90%).Conclusion The CMV infection rate in Qinghai province was lower.The quality of blood in Qinghai was better than that in other provinces in our country.

Objective To understand the status of the improper avademic behavior in medical journal,and to explore the prevention measures.Methods Total of 1 321 article in Zhongguo Yishi Jinxiu Zazhi were included in this study.Similarity detection system of Wanfang data and Google and baidu search engine were used to duplicate checking.The suspected existence of contributions for the improper avademic behavior,to check the whole paper of relevant original manuscript,and analyzed the characteristics of improper avademic behavior.Results One hundred and forty-five improper avademic behavior were found in 1 321 articles,accounted for 11.0％.The similarity of 58 paper were higer than 50.00％,accounted for 40.0％.Among them,the similarity of 10 papers were more than 90.00％,8 papers were 80.00％-89.99％,12 papers were 70.00％-79.99％,13 papers were 60.00％-69.99％,15 papers were 50.00％-59.99％,13 papers were 40.00％-49.99％,accounted for 9.0％,24 papers were 30.00％-39.99％,accounted for 16.6％,50 papers were 20.00％-29.99％,accounted for 34.5％.In 145 papers,copying accounted for 35.2％ (51/145),data forge or tamper accounted for 28.3％(41/145),breach of medical ethics and academic moral accounted for 28.3％ (41/145),fraud accounted for 6.9％ (10/145),a draft cast more accounted for 1.4％ (2/145).Original articles in the clinic were 92 papers,accounted for 63.4％;case reports were 28 papers,accounted for 19.3％;review were 25 papers,accounted for 17.2％.76 papers with a single author,accounted for 52.4％;38 papers with two authors,accounted for 26.2％,31 papers with more than three authors,accounted for 21.4％.Conclusions Contributions of suspected improper avademic behavior ratio is higher,and have certin characteristic.Medical journal editors should strengthen the responsibility consciousness,improve the ability to identify misconduct papers.

Objective To investigate the changes in serum leptin and adiponectin levels and their significance in patients with chronic hepa-titis B virus (HBV)infection.Methods One hundred and twenty -five patients with chronic HBV infection (44 HBeAg -positive cases, 31 HBeAg -negative cases of chronic hepatitis B,23 chronic HBV carriers,and 27 inactive HBsAg carriers)were recruited in the present study.Fifty -five healthy people served as the control group.Blood samples were collected at 8 to 9 AMof the next morning after overnight fasting.Blood glucose,blood lipids,renal and liver function parameters,fasting insulin,serum HBV markers,and HBV DNA concentration were determined,and HOMA -IR was calculated.Serum levels of leptin and adiponectin were measured by ELISA.Continuous data were expressed as mean ±SD,and Student′s t -test,analysis of variance,linear regression analysis,and multiple regression analysis were ap-plied in the analysis of all experimental data.Results There were no significant differences in serum levels of leptin and adiponectin be-tween patients with chronic HBV infection and the control group (P =0.480;P =0.321),even after stratification by gender (males:P =0.210 /0.895;females:P =0.404 /0.066).After patients with chronic HBV infection were divided into subgroups by HBeAg status,HBV DNA,or ALT level,there were no significant differences in serum levels of leptin and adiponectin between groups (leptin:P =0.820 /0.296 /0.212;adiponectin:P =0.268 /0.760 /0.224).Conclusion For patients with chronic HBV infection,infection status and viral replication level are not significantly correlated with serum levels of leptin and adiponectin.

Objective To explore the clinical value of tuberculous infection of T cells spot test (T-SPOT. TB)for diagnosing tuberculous pleurisy in immunocompromised patients. Methods The sensitivities of T-SPOT. TB,ADA, TB-DNA and tuberculosis antibodies to tuberculous pleurisy in 54 immunocompromised patients were compared.Among these patients,22 were retested by T-SPOT. TB after 2 months anti-tuberculosis treatment. Spot forming cells(SFCs) were compared before and after treatment. Results The diagnostic sensitivity of T-SPOT.TB (79.63%)was significantly higher than those of ADA (64.81%),TB-DNA (48.15%)and tuberculosis antibodies (55.56%) (P<0.05).22 patients were retested by the T-SPOT.TB after anti-TB treatment (2 months), the results showed that the positive rate of T-SPOT.TB was 90.91%,not significantly than previous tests (P<0.05).Both ESAT-6 and CFP-10 specific SFCs decreased significantly compared with those before anti-TB treatment (t=2.728 and 2.400,P<0.05 respectively). Conclusions T-SPOT.TB assay has satisfactory sensitivity and could be used in diagnosis of tuberculous pleurisy in immunocompromised patients.

Objective To analyze the condition of Rhodamine 123 staining of human bronchial epithelium in vitro during the wound-repair process induced by fluorouracil(5-FU). Methods The bronchial epithelial cells in normal and 5-FU injurious group were obtained by enzymatic digestion and analyzed by flow cytometry.1.PI staining to identify the percentage of cells in different cell phase;2.Rhodamine 123 staining in live cells was to contrast the percentage of negative cells in two groups. Results 1.Analysis of cell cycle:apoptotic cells in injurious group increased,cells in S+G-2/M phase almost disappeared,majority of the remain live cells of injurious group were in G-0/G-1 phase;with the recover of bronchial epithelium,cells in S+G-2/M phase increased;2.The percentage of Rhodamine 123 negative staining live cells in injurious group was higher than that of normal group,and with the recover of bronchial epithelium,this percentage lowered to nearly normal.Conclusion 5-FU can make the cycling cells apoptosis and reserve quiescent phase cells.Among them there were bronchial stem cells which had the capacity to efflux Rhodamine 123.Just the proliferation and differentiation of these stem cells regenerated bronchial epithelium.Rhodamine 123 staining to sort the remain cells after 5-FU treatment by FACS can be used to enrich and purify bronchial stem cells.

Objective To observe the application value of cardiac troponin I (cTnI),cardiac troponin T (cTnT) and creatine kinase MB (CK MB) for the early diagnosis of acute myocardial infarction (AMI).Methods The same serum sample was measured to detect the indexes of cTnI,cTnT and CK MB levels in 60 patients with AMI and 40 patients with UA.Comparison between AMI and UA was performed and all the indexes were analysed contrastively.Results The sensitivity of cTnI and cTnT was higher than that of CK MB,and their positive rates were 63.3%,46.7% and 18.3% respectively (P0.05) and the specificity of the three indexes were almost the same without significant difference.Conclusion cTnI and cTnT,as specific markers of cardiac damage,are more sensitive and specific in the early diagnosis of AMI.As a convenient,swift and accurate measurement method,cTnI has significant clinical value.

Objective To investigate the significance of serum cortisol and high‐sensitivity C‐reactive protein (hs‐CRP) test in elderly patients with neurasthenia .Methods A total of 160 elderly patients with neurasthenia in our hospital from January 2012 to June 2015 were selected as the research subjects (observation group) and 160 individuals undergoing the physical examination as the control group .The differences of serum BDNF ,CORT and hs‐CRP levels were compared between the two groups .The patients were divided into the obvious anxiety group and anxiety group according to the Hamilton Anxiety Scale (HAMD) .The differences of serum BDNF ,CORT and hs‐CRP levels were compared between these two groups ;meanwhile serum BDNF ,CORT and hs‐CRP levels after treatment in the patients with ideal curative effect were detected and the detection results were statistically compared with those before treatment .Results The BDNF and CORT levels in the observation group were significantly lower than those in the control group ,while the hs‐CRP level was significantly higher than that in the control group ;the BDNF and CORT levels in the obvious anxiety group were significantly lower than those in the anxiety group ,while the hs‐CRP level was significantly higher than that in the anxiety group ;the treatment outcome also showed that the BDNF and CORT levels after treatment in the patients obtai‐ning the ideal effect were significantly increased compared with the pre‐treatment levels ;while the hs‐CRP level was significantly decreased .Conclusion BDNF ,CORT and hs‐CRP are significantly correlated with neurasthenia occurrence and its severity ,which deserves to pay attention to in clinical practice .

Objective Toinvestigate the effect ofPBLmethod in the pediatrics'nurse teaching.Methods PBL teaching was practiced in trial group,while LBL teaching was practiced in control group.The writers made an analysis of this teaching by way of questionnaire and exam.Results Many students by PBL teaching accept PBL teaching,and improve their self-study ability,understanding ability,analyzing ability and so on.Conclusion PBL teaching method can effectively promote both the teaching quality in pediatric nursing and the students'study interest.