Objective To investigate the influences of Tangbikang on the oxidative stress in rats with diabetic penipheral neuropathy (DPN). Methods Diabetes rats were induced by intraperitoneal injection of STZ, 55 mg/kg and randomly divided into five groups: model group, methycobal treatment group, and Tang Bi Kang low, medium and high dosage treatment group. Body weight was examined every week. All rats were sacrificed at week 8 and the values of blood serum, superoxide dismutase, malondialdehyde, nitric oxide synthase and nitric oxide were detected. Results After treated with Tang Bi Kang, the eighth week's body weight, the levels of SOD, NOS and NO in the blood serum and ATP of sciatic nerve were increased greatly (P＜0.01 or P＜0.05) and the contents of MDA and AGEs and AR of sciatic nerve were decreased (P＜0.01), compared with the model group. Conclusion Tang Bi Kang may lighten the oxidative stress reaction of diabetic penipheral neuropathy and slow down the course of the diabetic penipheral neuropathy.

Objectives: To study the correlation between the dynamic expression of aquaporin-4 (AQP-4) around hematoma and the blood-brain barrier permeability after intracerebral hemorrhage in rats. Methods: Forty-two Sprague-Dawley rats were randomly allocated into sham-operation group, and groups of 6 hours, 12 hours, 1, 3, 7 and 14 days (n=6 in each group) after intracerebral hemorrhage. The intracerebral hemorrhage model in rats was established by injecting autologous arterial blood into caudate nucleus. Immunohistochemical method and Evans blue method were used respectively to detect the dynamic changes of AQP-4 around hematoma and the blood-brain barrier permeability in different time periods after intracerebral hemorrhage in rats. Results: (1) The expression of AQP-4 around hematoma increased gradually 12 hours after intracerebral hemorrhage, it increased significantly at day 1 and reached a maximum at day 3. It was slightly higher than normal level at day 7 and almost returned to normal at day 14 (P

Objective To investigate the significance of serum cortisol and high‐sensitivity C‐reactive protein (hs‐CRP) test in elderly patients with neurasthenia .Methods A total of 160 elderly patients with neurasthenia in our hospital from January 2012 to June 2015 were selected as the research subjects (observation group) and 160 individuals undergoing the physical examination as the control group .The differences of serum BDNF ,CORT and hs‐CRP levels were compared between the two groups .The patients were divided into the obvious anxiety group and anxiety group according to the Hamilton Anxiety Scale (HAMD) .The differences of serum BDNF ,CORT and hs‐CRP levels were compared between these two groups ;meanwhile serum BDNF ,CORT and hs‐CRP levels after treatment in the patients with ideal curative effect were detected and the detection results were statistically compared with those before treatment .Results The BDNF and CORT levels in the observation group were significantly lower than those in the control group ,while the hs‐CRP level was significantly higher than that in the control group ;the BDNF and CORT levels in the obvious anxiety group were significantly lower than those in the anxiety group ,while the hs‐CRP level was significantly higher than that in the anxiety group ;the treatment outcome also showed that the BDNF and CORT levels after treatment in the patients obtai‐ning the ideal effect were significantly increased compared with the pre‐treatment levels ;while the hs‐CRP level was significantly decreased .Conclusion BDNF ,CORT and hs‐CRP are significantly correlated with neurasthenia occurrence and its severity ,which deserves to pay attention to in clinical practice .

Objective]To study Xin'an physician Wu Cheng asthenic diseases related theory of ideas in Bujuji, in order to more comprehensively understand asthenic diseases,to broaden the thinking, enhance the clinical curative effect. [Method]From the academic source of asthenic diseases , syndrome differentiation and treatment,prescription medication,clinical academic value, make research on Wu's thought about asthenic diseases treatment. [Result]Wu Cheng combined with their own perception and experience of clinical evidence, first put forward“external damage caused by asthenic diseases”;Aiming at the asthenic diseases of the treatment,Wu Cheng creatively put forward“support solution method”, “supplementary care method”, “treatment of spleen yin”;Wu Cheng's life was exploring the asthenic diseases,the theory of asthenic diseases had good clinical academic value,not only for the development of the cause of disease theory,but also enriching the theory of health prevention.[Conclusion]Wu Cheng Bujuji rich in content and its syndrome differentiation of asthenic diseases, with much experience, as today, treatment based on the thought of clinical asthenic diseases treatment still has important guiding significance.

Adenoma ; diagnosis ; therapy ; Humans ; Male ; Middle Aged ; Parathyroid Neoplasms ; diagnosis ; therapy

Adult ; Humans ; Male ; Maxillary Sinus ; Tooth Eruption, Ectopic

Aim: To develop and compare the ultra-performance liquid chromatography( UPLC) and HPLC methods for the determination of dihydroflavonoids in Smilacis glabrae Rhizoma, and establish the quality evaluation system of the above-mentioned crude drug. Methods: Four dihydroflavonoids in the crude drugs collected from 15 localities were determined using the UPLC and HPLC methods, respectively. The resolution, sensitivity, precision, accuracy and the content determination results of the four compounds were compared between the two methods. Results: The UPLC method was more fast and sensitive than the HPLC method with no significant differences among the linearity range, precision, accuracy and the content determination results between the two methods. Conclusion: The developed HPLC method was proved practicable and reliable for the quality control of Smilacis glabrae Rhizoma. The UPLC method was provided to be a more sensitive, fast and solvent-saving method compared to HPLC and can be applied in the quality evaluation of Chinese medicines.

The core problem of the brain-computer interface (BCI) based on neural signal is estimating neural firing rate from a spike train and then using neural population decoding algorithm to decode movement trajectory.In this artical, we review the theoretical basis of both classic and current firing rate estimations and compare the advantages and drawbacks of these methods. At the same time we also review the decoding algorithm which using neural firing rate to decode movement trajectory in brain- computer interface: population vector algorithm, linear filter and kalman filter. At last, some results applying these estimators of firing rate to decode arm movement in BCI are introduced. The results show apparently different performance of the different firing rate estimators, while minimal differences are observed in the actual application of BCI.

Objective Herpes simplex virus is the pathogenic agent of human herpes simplex. There are two genotypes of herpes simplex virus, HSV-1 and HSV-2. The clinical manifestations of HSV-1 and HSV-2 overlap each other and it is difficult to differentiate them clinically. Methods developed based on genome differences are efficient ones to differentiate the two genotypes of HSV. In this study, we attempted to develop a new method to detection and genotyping human HSV in clinical samples. Methods Swab samples were collected from genital lesions of patients and placed in transport media. Samples were used to inoculate Vero cells. After appearance of the cytotoxicity, the infection mixtures were collected, and subjected to genomic DNA extraction. Based on the conservation and variation of gD of HSV-1 and HSV-2, a pair of primers amplifying both of them were designed and synthesized. Sequence of the virus were amplified and cloned into pMD-18T, and then the sequence was determined by DNA sequencing. The sequence was compared to all the known sequences in Genebank by using BLAST. According to the BLAST results, the genus and genotype of the virus was determined. Results A DNA fragment of about 200 bp was successfully amplified. This DNA fragment was cloned and sequenced. The sequence was compared with other known sequences. the results showed that this sequence had the highest homology to gD of HSV, indicating that the virus in the sample was HSV-2. The BLAST results also showed that there were some differences in the sequence of gD to those previously isolated. Conclusion DNA sequencing of PCR amplification products is an efficient and definite method to detect and genotype HSV-1 and HSV-2 which otherwise are difficult to differentiate clinically.

To study the changes in levels of tumor necrosis factor ? (TNF ?), interleukin 6 (IL 6) and interleukin 1? (IL 1?) produced by the peripheral blood mononuclear cells (PBMC) of patients with congestive heart failure (CHF), and to investigate the effects of captopril, one of angiotensin converting enzyme inhibitors in active form, on production of these cytokines. PBMC from 35 CHF patients and 12 healthy controls were isolated by density centrifugation with Ficoll Hypaque, and were cultured with captopril at 10 -4 ～10 -2 mol/L in the presence of lipopolysaccharide (LPS). After incubation for 24 hours, TNF ? and IL 6 contents in the culture supernatants were measured by an enzyme linked immunosorbent assay (ELISA), and IL 1? by radioimmunoassay (RIA). The results showed that PBMC from CHF patients released significantly more TNF ?, IL 6 and IL 1? compared with cells from controls ( P