Acupuncture Therapy ; Humans ; Meridians ; Muscles ; physiopathology ; Nervous System Diseases ; physiopathology ; therapy

Objective: To study the chemical constituents of Eleutherine americana. Methods: The compounds were isolated by various chromatographic techniques, including silica gel, TLC, sephadex LH-20, and semi-preparative HPLC, and their structures were identified by their physicochemical properties and 1H-NMR and 13C-NMR data. Results: Fifteen compounds were isolated from E. americana, which were germacrenin B (1), phaffiaol (2), 4,8-dihydroxy-3-methoxy-l-methylonthra-9,10-quinone-2-carboxylic acid methyl ester (3), 3-heptadecyl-5-methoxyphenol (4), ethyl linoleate (5), 3,5-dimethoxybiphenyl-4’-ol (6), karwinaphthol A (7), 5-hydroxylkarwinaphthol A (8), 3,4-dimethoxy-8-hydroxy-1-methyl-anthra-9,10-quinone-2-carboxylic acid methyl ester (9), isoeleutherin (10), eleutherin (11), isoeleutherol (12), naphtho-γ-lactone (+)-9-hydroxyeleutherol (13), senkyunone (14), and palmitoleic acid (15). Conclusion: Compounds 2, 4-7, and 13-15 were isolated from the genus for the first time. The compounds 3, 8, and 13 had strong vasodilator effects with diastolic rate of 85.3%, 81.8%, and 89.5%, respectively, which were basically equivalent to the positive drug of tanshinone IIA (86.3%).

Objective To determine the iodine content of common foods in different geographical areas (coastal city,coastal rural area,mountainous city and rural area) in Fujian Province and provide basic data for evaluation of dietary iodine intake.Methods In 2010,based on the types of food of the total diet study,one food sample(consumption rate is greater than 1％) was collected in coastal city(Taijiang),coastal rural area(Xiang'an),mountainous city (Sanyuan) and rural area(Mingxi).These foods including cereal,beans,potato,meat,eggs,milk,aquatic products,vegetables,fruits,sugar,beverages,liquor and seasoning,and so on 184 kinds of common foods.The iodine content of these food was tested by As (Ⅲ)-Ce4+ catalytic spectrophotometry.Results Among the 184 kinds of food tested,164 were not indicated food iodine content in the Chinese Food Composition Tables (2004).The iodine content of common food in Fujian Province in descending order were salt (30 000 μg/kg),aquatic products(341.4 μg/kg),eggs(255.9 μg/kg),dairy(106.7 μg/kg),meat(103.2 μg/kg),cereals (40.7 μg/kg),vegetables(30.7 μg/kg),beans(12.9 μg/kg),sugar(8.5 μg/kg),fruits(6.7 μg/kg),potatoes(2.4 μg/kg) and alcohol(2.1 μg/kg).The iodine content of kelp and laver was the highest in all the food,which was 314 780.1,176 956.5 μg/kg,respectively.The iodine content of food from animal(241.4 μg/kg) was much higher than that of the food from plant(25.4 μg/kg).The iodine content of common cereals,potatoes,beans,sea algae,meat,eggs and aquatic products was compared in the four areas,and the difference was not statistically significant (M =135.5,20.0,42.0,16.0,54.0,4.0,x2 =1.4,all P ＞ 0.05).The iodine content of vegetables and fruits was compared,and the difference was statistically significant (x2 =8.5,M =204.5,all P ＜ 0.05).Conclusion The iodine content of different foods is different.

The genetic diversity of three Tibetan herbs, i. e., Sang-Di, E-Dewa and Ye-Xingba (Tibetan names), was studied based on the field collection, specimen identification and DNA sequence analysis. Swertia hispidicalyx, Gentiana lhassica and Scrophularia dentata, as the original plants of the three Tibetan herbs, were collected and identified. The regions of ITS, matK, rbcL, rpoC1, trnL(UAA), psbA-trnH, atpB-rbcL, trnS (GCU)-trnG(UCC), rpl20-rps12, trnL(UAA)-trnF(GAA) and nadl 2nd intron were amplified and sequenced. The ITS regions of S. hispidicalyx and S. dentata were cloned and sequenced, and the sequences were classified into different genotypes. All the sequences were analyzed and compared with those of closely related species. Our studies may provide reference for the genetic diversity analysis and molecular identification of the three Tibetan herbs.
Genetic Variation ; Gentiana ; classification ; genetics ; Phylogeny ; Plant Proteins ; genetics ; Plants, Medicinal ; classification ; genetics ; Scrophularia ; classification ; genetics ; Swertia ; classification ; genetics ; Tibet

Objective To observe the ability of SD rat dental papillae cells forming dentin-like structure induced by millipore filter combined with transforming growth factor-β1 (TGF-β1). Methods The first passage SD rat dental papillae cells were enzymatically dissociated and centrifuged to obtain a cell mass.The cell mass was seeded on the millipore filter combined with TGF-β1. The complex was incubated for 6 d in vitro or transplanted under the renal capsule for 2 weeks. Then the differentiation of dental papillae cells on the filter and the formation of mineral tissue on the implant were analyzed. Results A layer of polarized columnar cells were observed along the surface of the millipore filter, with cell processes extending into the porous media. Dentin sialoprotein(DSP) and dentin matrix protein-1 ( DMP-1 ) were positive in these cells.After 2 weeks, tubular dentin matrix was deposited on the surface of the aligned cells. Scanning electron microscopy showed that the thickness of newly formed tubular dentin was consistent. DSP and DMP-1 were expressed in columnar cells, tubular matrix and the dental papillae cells adjacent to the filter. Conclusions The millipore filter combined with TGF-β1 could effectively recruit progenitors onto its surface and induce odontoblast differentiation, secrete matrix in a homogenous manner, leading to dentinogenesis.

Objective: To study the chemical constituents from Desmodium triquetrum and their antihyperlipidemic activities. Methods: The constituents of D. triquetrum were isolated and purified using various column chromatographies. Their chemical structures were elucidated using extensive spectroscopic methods. The lipid-lowering effects of the isolates were evaluated in HepG2 cells. Results: Nine compounds were obtained from the ethanol extract of D. triquetrum and determined to be 6'-. O-cis-p-coumaroyl-3,5-dihydroxyphenyl-β-. D-glucopyranoside (1), tadehaginoside (2), rutin (3), quercetin-3-O-β-. D-glucopyranoside (4), quercetin-3-O-β-. D-galactopyranoside (5), 6-. O-(E)-. p-hydroxy-cinnamoyl-β-glucose (6), 6-. O-(E)-. p-hydroxy-cinnamoyl-α-glucose (7), kaempferol-3-O-β-. D-rutinoside (8), and 3-. O-β-D-galacopyranosyl (6-1)-α-. L-rhamnosyl quercetin (9). Compounds 1 and 2 significantly reduced the intracellular content of total cholesterols and triglycerides. Conclusion: Compound 1 is a new phenolic compound and exhibits potent anti-hyperlipidemic activity. Additionally, compounds 6 and 7 are isolated from D. triquetrum for the first time.

ObjectiveTo investigate the occurrence of new cretinism cases and the prevalence of endemic goiter in iodine deficiency disorders(IDD) high-risk areas of Fujian province,so as to put forward target prevention and control measures for these areas.Methods Twelve counties from Xiuyu,Xiangan,Pingtan,and Dongshan were chosen into the survey by simple random sampling,searching for new cretinism cases were carried out in children under 10 years old.Two schools were chosen in every county and the thyroid volume of forty children aged 8 - 10 were determined by B-ultrasonography methods and their urinary iodine(UI) was determined by As3--Ce4+catalytic spectrophotometry in each school.Twenty women of child-bearing age aged 18 - 40 were chosen for collecting edible salt and urine samples,and the salt iodine content was determined using self-quantitative kit and their UI was also determined by As3--Ce4+ catalytic spectrophotometry.Results In the 4 high-risk counties,no cretinism cases were found.The goiter rate of children aged 8 - 10 was 3.6％(37/1027),and that in Dongshan county was 5.4％(13/240),which was higher than the national standards for eliminating IDD( ＜ 5％).The median urinary iodine(MUI) of children aged 8 - 10 was 175.3 μg/L,and the MUI of women aged 18 -40 was 152.7 μg/L.The coverage rate of iodized salt was 82.7％(382/462).ConclusionsNew case or suspected new case of cretinism is not discovered in the high-risk areas of IDD of Fujian province,and median urinary iodine level of people is in the adequate range.

Objective To analyze the monitoring results of patients with iodine deficiency disorders,and to find out the eliminating process of iodine deficiency disorders and the iodine nutritional status of population before adjustment of iodine level in edible salt in Fujian Province.Methods Thirty counties(cities,districts) were sampled by population proportion probability sampling in the whole Province in 2011; one primary school was selected from each of those 30 counties(cities,districts) ; 40 children aged 8-10 were selected from each of those 30 primary schools; thyroid volume of these children was examined by type-B ultrasound and the iodine level in edible salt from those 40 households was tested by the method of direct titration.Twelve urine samples of those 40 children were collected randomly,and urinary iodine level was tested by arsenic cerium catalytic spectrophotometry.Resident's per capita salt intake was calculated by the three-day-weighing method.In the village(where the school was in),5 drinking water samples were collected in the north,the south,the east,the west and the center of the village.If the water supply was centralized in the village,then 2 tap water samples were collected.Water iodine level was determined by arsenic cerium catalytic spectrophotometry.Three townships(towns,street offices)were selected in the vicinity of those schools; 5 pregnant and 5 lactating women were selected in each of those 3 townships (towns,street offices).Their urinary iodine level was determined by arsenic cerium catalytic spectrophotometry.Results Totally 1219 children aged 8 to 10 were examined,and their goiter rate was 4.92％(60/1219).Three hundred and sixty three urine samples were tested,and the median urinary iodine level was 223 μg/L.Among them,urinary iodine ＜ 50 μg/L accounted for 5.2％ (19/363),and ＜ 100 μg/L accounted for 14.6％ (53/363).The median urinary iodine level of pregnant women was 147.2 μg/L,and 52.0％(235/452) of them were less than 150 μg/L.The median urinary iodine level of lactating women was 134.1 μg/L.The consumption rate of qualified iodized salt was 94.4％ (1143/1211).Per capita daily salt intake was 6 g,and 81.4％ (293/360) of the residents' intake was less than 9 g.The median iodine content of drinking water was 6.2 μg/L,and 89.5％ (68/76) of them was less than 10 μg/L.Conclusions All indicators have met the national standard of eliminating iodine deficiency disorders in Fujian Province in 2011.But there is an iodine deficiency problem in pregnant women,and these women should be given extra iodine supplement.

Objective To investigate the meaning of PURA gene and its protein in nephridial tissue of the rats with endemic fluorosis of coal burning. Methods Thirty-six SD rats of 80 - 100 g, body weight were randomly divided into control group, low fluorosis group and high fluorosis group according to body weight, 12 in each group, the number of female and male in each group was the same respectively. The control group, Low fluorosis group and high fluorosis group rots were fed with 1.5,25.0,60.0 mg/kg fluoride content in feedstuff, to establish the animal model of fluorosis. Expressions of both mRNA and its protein of PURA gene in rat nephridium tissue, were determined by RT-PCR and immunohistochemistry after four-month experimental period. Results The expressions of PURA mRNA[(2.74± 1.06),(4.29 ± 2.11)] and its protein[ (28 827.91 ± 4801.94),(61 146.96 ± 4997.55)] in low fluorosis group and high fluorosis group was higher than that in the control group[ ( 1.13 ± 0.87), (7131.95 ± 1524.54), all P < 0.05]. And the expressions of PURA mRNA and protein in high fluorosis groups was higher than that in low fluorosis greup(all P < 0.05). Conclusion High fluoride can lead to the high expression of PURA gene mRNA and protein in the rat nephridium tissue exposed to sodium fluoride.

Objective:To evaluate the effects of periodontitis on the expression of apoptosis-related proteins in pancreas of rats with Type 2 Diabetes Mellitus.Methods:Spontaneously type 2 diabetic OLETF rats were randomly divided into 2 groups:diabetes with or without periodontitis(diabetes group and combination group).LETO rats with the same germline and the same age but having normal glucose tolerance were randomly divided into control group and periodontitis group.20 weeks after periodontitis were established,all the rats were sacrificed and the pancreas were pathologically examined by HE staining.The expression of Bax,Bcl-2 and Caspase-3 in the pancreas islet were detected by immunohistochemistry staining and semi-quantitative analysis.Results:The expression of Bax, Bcl-2 and Caspase-3 in the pancreas islet was no significant difference between control and periodontitis groups(P=0.324,P=0.091,P=0.852).Compared with diabetes group,the expression of Bax and Caspase-3 in combination group showed a significant increase(P=0.000,P=0.000),and the expression of Bcl-2 was significantly decreased(P=0.022).Conclusion:Under healthy conditions,periodontitis has no effect on the expression of apoptosis-related proteins in rat pancreas islet.However,in rats with diabe-tes,periodontitis may affect the expression of apoptosis-related proteins in pancreas islet.