Objective To investigate the effects of gemcitabine,a cytotoxic drug,combined with photodynamic therapy (PDT) in treatment of human pancreatic cancer xenograft in nude mice.Methods The animal model of human pancreatic cancer was developed by suturing small pieces of SW1990 tumors into the dorsum of nude mice.Sixty animal models were randomly divided into five groups with 12 each:control group ( without any treatment ), photosensitizer group ( 2 mg/kg of Photosan, without illumination ),chemotherapy group (receiving 50 mg/kg of gemcitabine i.p. on day 0,3,6 and 9 after transplantation),photodynamic group (2 mg/kg photosan combined with laser irradiation) and combination group (50 mg/kg of gemcitabine and 2 mg/kg of photosan combined with laser irradiation). The tumor sizes were measured twice every week.All mice were sacrificed after 21 days.The tumor volume was calculated,and inhibitory effect and changes before and after treatment were analyzed.Results The tumor was grown bigger in control,photosensitizer and chemotherapy groups (all P value ＜0.05).On day 6,9,12,15,18and 21, the tumor size was significantly smaller in photodynamic group than those in control and photosensitize groups.While the tumor size on day 18 and 21 was smaller in combined group than those in photodynamic group(all P value ＜0.05). The tumor mass was (0.29 ± 0.20) g in combined group,which was lower than that in photodynamic,control,photosensitize and chemotherapy groups[(0.69±0.23) g,(1.65±0.21) g,(1.62±0.12) g,(1.37±0.19) g,respectively,P＜0.05].The inhibitory effect were 82.420%00 and 58.18% in combined group and photodynamic group,respectively(P＜0.05),while there was 1.80% and 17.00% in photosensitize and chemotherapy groups,respectively.Conclusions Photodynamic therapy has significant and short anti-tumor effect,but it can be significantly enhanced by combined with small dose of gemcitabine.

Objective To investigate the anti-tumor effects of photodynamie therapy(PDT)on human pancreatic cancer xenograft in nude mice and the time-effect relationship of PDT.Methods The animal model of human pancreatic cancer was developed by suturing small pieces of SW1990 tumors into the dorsum of nude mice.When the size of the tumors increased to 0.8～1.0 cm.36 mice were randomly divided into three groups:control group(no treatment),photosensitizer group(Photosan 2mg/kg,abdominal cavity injection),photodynamic group(Photosan 2 mg/kg injection+laser irradiation).Each group included 12 mice.The tumor sizes were measured twice per week and the weight8 and volumes of the tumors were measured,and the tumor inhibitory rate was calculated three weeks later.Results The tumor volume of photodynamic group was (0.22±0.12)mm3 at the 6th day,which was significantly smaller than(0.43 s0.18)mm3 of control group and(0.39±0.15)mm3 of photosensitizer group(P＜0.05).15 days later,the tumor volumes of PDT groups increased.21 days later.the weight of the tumors in photodynamie group was(0.69±0.23)g,which was significantly lower than(1.65 ±0.21)g of control group and(1.62±0.12)g of photosensitizer group(P＜0.05).The tumor inhibitory rate in photodynamic group was 58.18%,which was significantly higher than that of photoseasitizer group(1.8%,P＜0.05).Conclusions Photodynamie therapy had significant anti-tumor effect on human pancreatic cancer with quick-acting efficacy,but photodynamie therapy alone exeaed efficacy only in the shoa term.

Objective To evaluate the clinical value of preserving the intercostobrachial nerve after radical operation for breast carcinoma.Methods The clinical data of patients with breast cancer were analyzed retrospectively,all cases were divided into the observation group and the control group.all cases were followed up after operation from six months to six years in order to observe the abnormal sensation of the skin and the recurrence rate.Results The abnormal sensation of the skin:1 case in the observation group(4.5%),17cases in the control group(73.9%),Two groups have significant difference(x2=20.79 P＜0.01),both groups had not found partial recurrence.Conclusion Preseving the intereostobmchial nerve in modified radical mastectomy for patients with breast cancer,dichat increase the partial recurrence,can increase the life quality of patients after operation,decrease the abnormal sensation of the skin.promote the health both body and mind.

Objective To explore the early diagnostic marker and mechanism of the injury of in- testinal mucosal barrier induced by intestinal ischemia-reperfusion in rats.Methods Sixty male Wistar rats were randomly divided into six groups:the sham operation group(SO),the ischemia 15 minutes group(A),the ischemia 45 minutes group(B),the ischemia 45 minutes plus reperfusion 2 hours group (C),the ischemia 45 minutes plus reperfusion 6 hours group(D),and the ischemia 45 minutes plus reperfusion 12 hours group(E).Using clamping and then releasing superior mesenteric artery the model of intestinal ischemia-reperfusion in rats was made.The sham operation group only underwent laparotomy. At different time points after ischemia and reperfusion the levels of serum CK,LDH,D-lactate and intes- tinal fatty acid binding protein(IFABP)in each group were examined.The morphological changes of in- testinal tissues were observed with light microscopy.Results Compared with group SO,the level of se- rum IFABP in group A was(374.74?48.85)pg/ml,significantly higher(P＜0.01),but the level of CK,LDH and D-lactate had no significant difference(all P＞0.05).In group B,the level of CK was (1090.40?187.51)u/L,peaking at 45 minutes after ischemia,meanwhile,D-lactate and IFABP levels were significantly increased(P＜0.01,respectively).In group C,D-lactate and IFABP were (2.51?0.19)?g/ml and (1601.42?286.81 )pg/ml,respectively,peaking at 2 hours after reperfusion (P＜0.01).At 6 hours after reperfusion,compared with ischemia 45 minutes,CK level was significantly de- creased(P＜0.01),LDH had no significant difference(P＞0.05),but the levels of D-lactate and IF- ABP were(2.03?0.24)?g/ml and(1443.76?174.52)pg/ml,respectively,all sustained a high lev- el(P＜0.01 ).At 12 hours after reperfusion,D-lactate and IFABP levels were gradually decreased(P＜0.01).At 45 minutes after ischemia the morphological changes of intestinal mucosa could be observed. At 6 hours after reperfusion part of the mucous layer appeared necrotic,some intestinal mucosal cells shed to enteric cavity,and submucous layer had hyperemia and edema obviously.Injury scores of intestinal mucosa were significantly correlated to the serum level of D-lactate and IFABP,correlation coefficients were 0.456,0.612(P＜0.01).Conclusion The monitoring of serum IFABP combined with D-lac- tate is a early,sensitive and specific biochemical marker in the diagnosis of intestinal mucosal barrier in- jury after ischemia-reperfusion.

Object To study the anti-arrhythmia effect and acute toxicity of flavonoid from Erysimum cheiranthoides L. Methods To study the anti-arrhythmia of the flavonoid by determination of the ECG of rabbits. To study the acute toxicity effect of the flavonoid by Karber's method. Results The results showed that the flavonoid could remedy to tachycardia caused by Adriamycin and it could remedy to bradycardia caused by Verapamil. Acute toxicity showed that LD 50 of flavonoid is 311.7 mg/kg, 95% CI is 289.9—339.5 mg/kg. Conclusion The flavonoid can rectify tachycardia due to various reasons with very little toxicity.

AIM: To establish HPLC fingerprint of Ramulus cinnamomi.This fingerprint was expected to be standards of quality control and identification of the Chinese crude drug. METHODS: The HPLC method was used,chromatography condition were Kromasil C_(18)(250 mm?4.6 mm,5?m) column with gradual mobile phase of acetonitrile-0.5% acetic acid,UV detection wavelength at 280 nm and column temperature at 25(?C) with the flow rate of 1 ml?min~(-1). RESULTS: The retention time of Cinnamaldehyder,Cinnamic acid,o-Methoxycinnamic acid and Coumarin in Ramulus cinnamomi were determined.The fingerprint showed high similitude in samples from different regions. CONCLUSION: The HPLC fingerprint of Ramulus cinnamomi.can be used as a standard of quality control and identification of the Chinese crude drug.

Objectives: To investigate the treatment effect of enteral nutrition rich in diet fiber in diarrhea because of bacteria disorder after severe brain injuries.Methods:20 cases with severe brain injuries were supported with enteral nutrition rich in diet fiber after the occurrance of diarrhea because of bacteria disorder as clinical treatment group.20 cases with the same conditions were treated with traditional methods as control.Results: Diarrhea of all patients in clinical treatment group had been controlled during 7-10 days,and the feeding quantities reached total calories.The nutrition state was improved,and the body internalenvironment was stable during the 20 days. Conclusion: Enteral nutrition rich in fiber can treat diarrhea caused by bacteria disorder after severe brain injuries.

[Objective]To analyse the operative complications in total hip arthroplasty.[Method]Sixty-two patients (62 hips) undergone total hip arthroplasty from March 1998 to November2009,were grouped according to the different causes of the operative complications.[Result]Based on the analysis of all cases,it was found that the most common causes resulting in the failure of the surgery were surgical technichque and fracture,anesthesia,osteoporosis,diabetes mellitus,and cerebral hemorrhage.Complications in all 5 cases were sudden death in 1,femural fracture in 1,unequal-length lower timbs in 1,and postoperative dislocation in 2.[Conclusion]Total hip arthroplasty has been extensively used as an effective procedure for the reconstruction of hip joint.Long-term excellent clinical results are related to preoperative preparation,patient matched fact,and the experience and surgical technique of the surgeon are also of importance to the final outcome.

Objective To prove into the effect of parental involvement in nursing to discharged children with metabolic syndrome.Methods Forty-six children with metabolic syndrome were equally divided into the observation group and the control group according to the order of admittance.Both groups accepted health education,guide to lifestyle and so on.After leaving hospital,the observation group accepted parental involvement in nursing,while the control group accepted physical examination regularly.Then the lifestyles,physical indicators,blood pressure and biochemical indicators of both groups were compared.Results The lifestyle of the observation group was significantly greater than that of the control group(All P<0.01).The observation group had greater declines in waist,blood pressure,TC,TG and LDL-C and a greater increase in HDL-C than those of the control group.Conclusions Parental involvement in nursing can improve the lifestyle of children patients,which is good for the declines of their weigh and blood lipid.This treatment can achieve the goal of control and treatment of children metabolic syndrome.

Objective: To evaluate the quality consistency of the original product ( DIOVAN ) and generic valsartan capsules ( VSC) . Methods:The dissolution of the two capsules in four media was monitored by the UV method described in Chinese Pharmaco-poeia ( ChP 2010 edition) . The f2 similarity factor approach was used to evaluate the dissolution similarity between DIOVAN and VSC and the production quality of the enterprises. Meanwhile, the homogeneity in the same batch was studied by dissolution precision and the reproducibility in the different batches were also evaluated by a similar equivalent limit method to show the production quality of the manufacturers. Results:The dissolution of VSC and Diovan in pH 6. 8 phosphate buffer medium was both above 85% in 15 min. The f2 similarity factors in the other three media ( water, pH 4. 5 acetate buffer and 0. 1 mol·L-1 HCl) were all above 50. The relative standard deviation ( RSD) of precision in the same batch was less than 10%. Among the different batches, dissolution limit value ( Q) was within the range of the upper and lower limit value of probability levels (δ) . Conclusion:The f2 similarity factor results indicate the in vitro dissolution of the reference drug ( DIOVAN) and generic drug ( VSC) is consistent in the four media. The production quali-ty of generic manufacturer is also good with promising homogeneity and reproducibility evaluation results.