Objective:To prepare loxoprofen sodium sustained release pellets, and investigate the in vitro drug release behavior. Methods:Loxoprofen sodium loaded pellets were prepared by extrusion-spheronization technology, and the sustained release pellets were prepared with Eudragit RL 30D and Eudragit RS 30D as the sustained release coating film materials. The drug release behavior of loxoprofen sodium sustained release pellets in vitro was studied. Results:Eudragit RL 30D and Eudragit RS 30D with the ratio of 20 ∶80 was used as the sustained release coating film materials, the coating weight was 20%, the plasticizer content was 10%, and the content of talc was 45%. The in vitro release of loxoprofen sodium from the sustained release pellets was steady and entire in 12 h. Conclusion:The release behavior of loxoprofen sodium sustained release pellets is quite satisfactory. And the preparation technology may be used in the industrial production.

Animals ; Burns ; physiopathology ; therapy ; Genetic Engineering ; Humans ; Hyperbaric Oxygenation ; Tissue Engineering ; Wound Healing

Anterior Cruciate Ligament ; surgery ; Anterior Cruciate Ligament Injuries ; Anterior Cruciate Ligament Reconstruction ; methods ; Humans ; Knee Injuries ; surgery

Type 1 diabetes, a complex genetic disease, is determined by both genetic and environmental factors. After excluding the five susceptibility loci discovered by genetic linkage studies and candidate-gene association studies, more than 30 new susceptibility loci have been found to be related with type 1 diabetes as a result of genome-wide association study.

Objective This study was performed to find the association of the severity of coronary artery disease with ordinary risk factors for CHD and left ventricle heart function in patients with CHD. Methods Selective coronary angiography were carried on 305 consecutive patients admitted to our hospital from December 2008 to April 2009. The severity of coronary disease was accessed by the Gensini scoring system. These patients were divided into two groups, patients with CHD group (251 cases) and control group (patients without CHD) (54 cases) , to compare the difference of ordinary risk factors for CHD between the two groups. Multiple linear regression analysis was used to analyze the association of the severity of coronary artery disease with ordinary risk factors for CHD. We also used linear correlation analysis to investigate the association of the severity of coronary artery disease with left ventricle ejection fraction in patients with CHD. Results The two groups had statistically difference on age, sex, smoking index, hypertension prevalence rate, serum glucose abnormal prevalence rate, diabetes prevalence rate, and metabolic syndrome prevalence rate( P < 0.05). We found that age (β_1 =0.251, P <0.01), sex (β_2 =0.235, P < 0.01), LDL-C concentration (β_3 = 0.241,P <0.01), duration of diabetes (β_4 =0.226, P <0.01) went into multiple linear regression model. We also found the severity of coronary artery disease had a negative correlation ( r = -0. 362, P <0.01) with left ventricle ejection fraction in patients with CHD. Conclusion When we decide to cany out coronary angiography on susceptible patients, we should consider their age, sex, smoking index, whether had hypertension, serum glucose abnormal, diabetes, metabolic syndrome or not. Age, sex, LDLC concentration, duration of diabetes affect severity of coronary artery stenosis independently. Left ventricle ejection function will decrease as coronary atherosclerosis continues.

Infectious mononucleosis(IM) was one of the clinical common diseases, caused by epstein-barr virus infection in more than 90%patients. Changes of the surface antigen of the B cells with EB virus infection caused strong immune response of T lymphocytes, besides, helper T lymphocyte, dendritic cells, NK cells also played an important role in the pathogenesis of IM. The incidence of IM was not only closely related with social environment in childhood, but also closely with the number of siblings, birth order, and sunshine factor. In addition to the typical clinical manifestations, IM also can cause a variety of complications, such as spleen rupture and necrotizing mediastinitis , and could be life-threatening. Auxiliary examination means such as state of peripheral blood lymphocyte apoptosis, plasma proteomics, cerebrospinal fluid analysis, load test of EB virus can help to diagnose. Application of antiviral drugs was wider, but hormone was still controversial. Vaccine research progress had been slow, but there were vaccines for human test, which will provide effective measures for the prevention of IM.

This paper introduced the medical device classification in China and USA. Through the comparison between the two systems, several problems in Chinese classification system were exposed. To the end, some suggestions were proposed referred to the classification system of USA.
China ; Device Approval ; standards ; Equipment and Supplies ; classification ; United States

Burns ; Evidence-Based Medicine ; Humans

Evidence-Based Medicine ; Humans ; Resuscitation ; Shock ; therapy

Objective To investigate the effects of intravenous injection of α-crystallin on retinal ganglion cells (RGC) and some important organs of the Long Evans rats. Methods RGC were retrogradelabeled by fluorogold through bilateral superior colliculus and lateral geniculate body for seven days before optic nerve crush injury. Twenty-three Long Evans rats were used for this study, including three rats of normal control group and 20 rats of experimental group. Twenty rats were randomly divided into saline control group and three α-crystallin injection groups, which received tail vein injection of 1.25 ml isotonic saline and three different concentrations (1 × 10-2 , 1 × 10-1 and 1 g/L) of α-crystallin respectively, once every two days and totally seven times. After two weeks, the labeled RGC were counted, and the pathological changes on liver, kidney, brain, spleen and the lungs were investigated. Results Compared with the normal control group, although the number of RGC markedly decreased after two weeks of optic nerve crush injury in every group, the number of RGC in α-crystallin-treated groups was more than those in the saline control group. There were 2074± 150 RGC per mm2 in normal control group, 85 ± 15 RGC per mm2 in saline control group, 124±26 RGC per mm2 in 1 × 10-2 g/L α-crystallin group, 128± 31 RGC per mm2 in 1 × 10-1 g/L α-crystallin group, 164 ± 20 RGC per mm2 in 1 g/L α-crystallin group (F= 18. 660,P＜0. 01). No congestion, swelling, inflammation and other pathological changes were found in liver,kidney, brain, spleen and lung. Conclusions Intravenous injection of α-crystallin protein has protective effects on RGC after the optic nerve crush injury, and no significant effects on important organs.