Objective To research the effect of nimodipine in the prevention and treatment of delayed facial palsy after microvas-cular decompression .Methods A retrospective analysis was taken from January 2009 to March 2012 ,193 cases of patients with fa-cial spasm undergoing MVD in our department .According to whether applied vasodilators after operation ,all cases were divided into two groups :the experimental group(72 cases)with the treatment of nimodipine for two weeks ;the control group(121 cases) ,and not treated with nimodipine .The follow-up time was 6-12 months .Observe and compare the incidence ,level of delayed facial paraly-sis onset time and disease duration time between two groups .Results In the experimental group had 6 cases of late facial paralysis , the incidence was 8 .3% (6/72) ,onset time was (14 .5 ± 5 .2)days ,disease duration was (41 .3 ± 14 .4)days .The incidence of control group was 9 .1% (11/121) ,onset time was (12 .2 ± 7 .4)days ,disease duration was (55 .7 ± 36 .4)days .There was no significant difference between groups(P>0 .05) .According to House-Brackman classification ,there were 4 cases of grade Ⅱ and 2 cases of grade Ⅲ in experimental group .In control group ,there were 2 cases of grade Ⅱ ,6 cases of grade Ⅲ ,3 cases of grade Ⅳ .The differ-ence was statistically significant (P<0 .05) .Conclusion The delayed facial paralysis is influenced by a variety of causes .Vasodila-tor drugs can reduce the incidence of delayed facial paralysis ,and play a certain role in promoting facial paralysis restored .

Objective:To establish the experimental nude mouse model of endometriosis for human eutopic endometrium.Methods:Female nude mouse aged 6~8 weeks were selected.Nude mouse in experimental group(n=20)were implanted with the endometrium of patients with endometriosis or without endometriosis into pelvic and abdominal cavities.Nude mouse in control group(n=3)were implanted with the greater omentum.Nude mouse were killed at 5th,15th,and 30th day after implantation for observ ation of the growth of endometriotic lesions.The morphological and histological changes of endometriotic lesions from diferent sources and at diferent time points of growth were examined by light microscopy.Results:The achievement ratio of transplantation was 100%.At the 5th day,endometriotic lesions were found in the pelvic and abdominal cavities,and the adhesion to mice tissues was already tight.There were nascent vascular net between endometriotic lesions and mice mesepithelium.Endometrial glands around with stroma cells were found under light microscope.At the 15th day,endometriotic lesions mixed with mice tissues.Endometriotic lesions presented parenchymatous and cystiform.A lot of endometrial glands and stroma cells with rich blood supply were seen under light microscope.At the 30th day,endometriotic lesions became atrophied,and the adhension was serve partly.Endometriotic lesions presented severe fibrosis and some endometrial cells were not in integrity under light microscope.In the control group,some of the greater omentum adhered to the incisal opening of abdominal wall,while the others were free and atrophied or merged gradually.There was no change in the constructed greater omentum except for some inflammatory reaction.Conclusions:Nude mouse model of endometriosis was good for studying the endometria cell proliferation,neovascularization,hormone,immune response and so on,since the implanted endometrium remain the original morphological and histological features.It is a perfect model of endometriosis because of its significant and steady character,low cost,simple operation and short observation cycle.

AIM:To compare the effect of recombinant human epidermal growth factor eye drops and deproteinized calf blood extract eye drops on corneal edema after phacoemulsification.METHODS:Totally 72 cases (72 eyes) of patients undergoing phacoemulsification were selected and divided into the observation group and the control group by random number table method.After surgery,the observation group were treated with deproteinized calf blood extract eye drops while the control group were treated with recombinant human epidermal growth factor eye drops.The degree of corneal edema,subjective symptom score,corneal endothelium count,changes of corneal thickness and postoperative visual acuity recovery were compared between the two groups at different time points after surgery.RESULTS:Corneal edema in the two groups was significantly milder at 1wk after surgery than that on the 1st day after surgery (P<0.001).There was no significant difference in the degree of corneal edema and test results of visual acuity between the two groups at 1wk after surgery (P>0.05).Compared with 1d after surgery,the subjective symptom score and corneal thickness of the two groups significantly decreased on the 7th day after surgery (P<0.05).At 7d after surgery,the above indexes were significantly better in the observation group than the control group(P<0.05).At 1mo after surgery,the corneal endothelium count was significantly larger in the observation group than the control group (P<0.001).CONCLUSION:Both of recombinant human epidermal growth factor eye drops and deproteinized calf blood extract eye drops can significantly relieve corneal edema and improve visual acuity of patients after phacoemulsification.However,the latter has obvious advantages over the former in the repair of corneal endothelial cell injury after surgery.

This paper was to explore the application of case based learning method in medical psychology course for medical undergraduates.According to curriculum standard,nine cases were compiled which matched six articles,forming the complete instructional design.We followed the five steps of scientific teaching stage.The preliminary results showed that the case teaching was helpful to improving the medical students' knowledge acquisition,and their ability to master and solve practical problems.

Cricoid Cartilage ; surgery ; Humans ; Laryngectomy ; methods ; rehabilitation ; Larynx ; Recovery of Function

High density lipoprotein ( HDL ) has been considered as an important mediator in favoring cardioprotective effects .However , recent studies revealed that HDL-C-raising therapeutics alone failed to reduce the risk of cardiovascular diseases , suggesting that the HDLfunctionalitymay be more critical for its cardioprotective properties than the simple HDL-C levels.microRNAs ( miRNAs) have been identified as the novel regulators of lipid metabolism and played essential roles in the key steps of reverse cholesterol transport, involving in HDL biogenesis , cellular cholesterol mobilization , hepatic HDL uptake and excretion.The intensive research on lipid metabolism-related miRNAs may provide further clarification on the molecular regulatory mechanisms for HDL-mediated reverse cholesterol transport and plasma HDL-C levels, advancing our knowledge on the effects of HDL in pathogenesis and progression of cardiovascular diseases.

Objective Through observing the effect of low-dose T-2 toxin on chondrocyte,to study the molecular mechanism of cartilage damage.Methods The primary chondrocytes were isolated from articular cartilage of d 1-2 Wistar neonate rats through enzymatic digestion.Different doses (0.005,0.010,0.100 μg/L) of T-2 toxin were added after 24 h in vitro culture.The survival rate of chondrocytes was detected with Trypan blue staining.Echylosis (matrix metalloproteinase,MMP1) was analyzed by immunohistochemistry.The damage of articular chondrocyte was observed by transmission electron microscope.Results ①Cell morphology of in vitro cultured chondrocyte:the newly isolated chondrocytes were spherical.After 24 hours,the adherent cells gradually began to stretch the triangle or polygon;the nucleus was large and round;the cell was clear and transparent,containing secretory granules.②Cell proliferation:T-2 toxin had a significant inhibitory effect on chondrocyte proliferation,the higher the concentration of T-2 toxin,the significant the inhibitory effects [0.000 μg/L (control) group:3.45 × 108/L,0.005 μg/L T-2 toxin group:3.45 × 108/L,0.010 μg/L T-2 toxin group:2.06 × 108/L,x2 =9.554,P ＜ 0.05].③Immunohistochemical observation:dysplasia,nucleus condensation and membrane rupture were observed in T-2 toxin treated group,brown staining was observed in all groups at varying degrees.The deepest staining was in 0.005 μg/L T-2 toxin group,with the strongest secretion of MMP1;with increasing doses of the toxin,the damage to cartilage cells was severe,MMP1 secretion was less,staining was weak,and the weakest staining was in the 0.100 μg/L T-toxin group.④Under transmission electron microscopy:in control group,cytoplasm was rich in rough endoplasmic reticulum,nuclear membrane and cell membrane were clear;in 0.005 μg/L T-2 toxin group,the cell nucleus showed pyknosis,organelles were decreased in cytoplasm;in 0.100 μg/L T-2 toxin group,the microvilli was dropped out of cartilage surface,nuclear changes were obvious,and mitochondria was myeloid degeneration;rough endoplasmic reticulum was degranulation and expansion into cystiform,chondrocytes were apoptosis occasionally,the cell nucleus showed pyknosis,and the formation of high-density plaque.Conclusion Low dose of T-2 toxin could damage the primary cultured articular chondrocyte in vitro.The results have showed that there are damaged cytostasis,chondrocyte degeneration,necrosis and apoptosis.

HA1 1 7,as a multiple drug resistance(MDR)gene,has recently been discovered.It may mediate the MDR of tumor through Bcl-2 anti-apoptotic pathways.HA1 1 7 gene exists high expression in leuke-mia,lymphoma,breast cancer,neuroblastoma,colon adenocarcinoma and so on,and its overexpression is closely associated with the MDR and poor prognosis of these tumors.

Extranodal NK/T-cell lymphoma,nasal type (ENKTL) is a aggressive lymphoma which arises from NK cells or T cells and has a poor prognosis.This article mainly reviewed and focused on hematological stem cell transplantation in treating ENKTL.

Objective To investigate clinical value of the emergency endoscopic sclerotherapy and tissue adhesives embolization on patients with gastroesophageal varices bleeding. Methods A retrospective analysis of 52 cases of esophageal variceal bleeding in patients with clinical data was performed. Of which,22 patients were given conservative treatment alone and they were served as conservative treatment group. Another 30 patients were given the emergency endoscopic sclerotherapy and/ or tissue adhesive embolization besides the basis of conservative treatment,and they served as endoscopic treatment group. The data of hospitalization and mortality,rebleeding rates were recorded during hospitalized time and 1 year off hospitalization. Results The indices of hospitalized periods in endoscopic treatment group was( 16. 2 ± 1. 5)d,shorter than that in conservative treatment group((17. 6 ± 2. 1)d,P = 0. 53). The mortality and rebleeding rate in endoscopic treatment group in followed up periods were 3. 3% and 6. 6%,lower than those in conservative treatment group (9. 1%,13. 6%),and the comparison of relative risk RR of two groups of mortality and rebleeding rate were 2. 76 and 2. 06. Conclusion The therapy of emergency endoscopic sclerotherapy and tissue adhesives embolization of bleeding esophageal varices shows a good effect and it is worth recommending.