Objective:To observe the effect of etanercept on bleomycin-induced pulmonary fibrosis in mice and its possible mechanisms.Methods:Thirty male SPF Kun ming mice were randomly assigned to control group ( intratracheal saline injection ) or model group ( intratracheal injection of bleomycin 5 mg/kg ) or intervention group ( intratracheal bleomycin injection followed by etanercept 3 mg/kg intraperitoneal administration twice a week )(n=10 group).Lung tissue were taken after the sacrifice of the mice with 28 days of molding.The content of hydroxyproline of the right lung samples was measured by alkaline hydrolysis method ,while the left lungs were fixed in 4%paraformaldehyde and embedded in paraffin with the sections stained with hematoxylin -eosin or Masson′s trichrome for pathological examination .Total tissue protein was extracted from lung homogenates to detect the expression of NF -κB and phosphorylation of NF-κB( P-NF-κB) by Western blot.Results:The content of hydroxyproline in lung tissue of mice of intervention arm was decreased significantly compared with the model arm ( P<0.01 ).The alveolar structure of the intervention arm maintained integrity with alveolar septum slightly thickened.The inflammation and fibrosis scores were decreased significantly with noticeable greater improvement of pulmonary fibrosis than the model arm (P<0.01).In addition,the protein phosphorylation of NF-κB in the lung tissues was remarkably decreased in the intervention arm ( P<0.01 ).Conclusion: Etanercept can inhibit the phosphorylation of NF-κB and reduce lung inflammation and fibrosis caused by bleomycin in mice ,which may be useful in the treatment of pulmonary fibrosis.

[Objective]To investigate the correlation of sagittal parameters in L5~S1 isthmic spondylolisthesis and its clinical manifestation.[Method]Seventy-six patients with L5~S1 isthmic spondylolisthesis were treated between January 2000 and December 2005,there were 32 males and 44 females with the age between 12 and 68 years.The lateral standing radiographs of the spine and pelvis were analyzed retrospectively.Radiographic parameters measured including pelvic incidence(PI),sacral slope(SS),lumbar lordosis(LL),lumbosacral angle(LSA)and grade of spondylolisthesis.All measurements were done by the same individual and compared to those of a cohort of 30 normal subjects.Students test was used to compare the parameters between the curve types and Pearsons correlation coefficients were used to investigate the association between all parameters(P

The purpose of this paper was to evaluate the relationship between the thrombosis and secretory duct dilation, lesion size, clinical types, nature (primary or recurrent) and duration of illness in the development of ranula. A total of 229 cases of sublingual gland cysts were treated with surgical resection from Jan. 1990 to Feb. 2010. The patients' data were investigated on histopathological findings, size of ranula, the clinical types, nature of ranula (primary or recurrent) and duration of illness. Sections from the paraffin-embeded blocks were HE-stained. CK expression was immunohistochemically detected. Among 229 cases the incidence of venous thrombosis was 58.52%. The incidence of venous thrombosis with or without duct dilation was 73.25% and 26.39% respectively, with a significant difference between the two groups (P<0.005). The incidence of venous thrombosis of ranulas with diameter larger or less than 3 cm was 72.22% and 46.28% (P<0.005). The incidence of venous thrombosis of oral ranula, plunging ranula and mixed ranula was 49.37%, 77.19% and 85.71% respectively, with a significant difference found between oral and plunging or mixed ranula (P<0.01). The incidence of venous thrombosis in ranula patients with duration of illness longer or less than 3 months was 69.77% and 51.75% (P<0.01). The incidence of venous thrombosis with recurrent and primary ranulas was 51.85% and 64.85%, without a significant difference noted between them (P>0.05). It is concluded that the formation of venous thrombosis was related to the dilation of secretory duct, lesion size, clinical types, duration of lesion but formation of venous thrombosis was not related to the nature (primary or recurrent) of ranulas.

Objective To construct a mifepristone inducible recombinant adenovirus carrying enhanced green fluorecence protein for future gene therapy in vivo.Methods Full length red fluorescent gene and mifepristone regulator cassette was subcloned into PDC313 shuttle plasmid.The positive clone was identified by sequencing.Recombinant adenovirus were produced after cotransfection of the shutter vector PDC-EGFP and adenovirus DNA helper plasmid pBHGloxE1,3Cre into 293 cells.The recombined adenovirus was purified by CsCl density gradient centrifugation and its titer was determined by end point dilution assay.Fluorescence microscopy and FCM were confirmed by the activation of this regulatable recombinant adenovirus vector in infected cells.Results The adenovirus vector containing enhanced green fluorecence protein gene was identify by PCR.The virus titer of Ad-RUEGFP was 6.6?1010pfu/ml.Without the absence of mifepristone,no significant enhanced green fluorecence protein activation was observed,whereas in the presence of mifepristone activation of the enhanced green fluorecence protein was positive correlation with the inducer under definite range.Conclusion A replication-defective recombinant adenovirus vector containing enhanced green fluorecence protein was successfully constructed,which can be inducible by mifepristone.

Objective Through testicular biopsy and detection of serum sex hormone levels in patients with azoospermia,to explore the relationship between spermatogenic function of testis,testicular volume and serum LH and FSH levels.Methods 80 cases of azoospermia patients adimitted from Jan.2012 to Dec.2014 were collected as the research object.Immunization method was used to detect the serum sex hormone leve,testis model was adopted to detect testicular volume,and routine disinfection was given to make testicular biopsy.According to testicular biopsy results,patients were divided into normal spermatogenic function group (group A),spermatogenic dysfunction group (group B),sertoli-cell-only syndrome group (group C);percutaneous sperm group (group D),and puncture azoospermia group (group E).At the same time,50 cases of healthy male were selected as the control group and they received sperm routine examination and sex hormone detection.Correlation between spermatogenic function of testis,testicular volume and serum LH and FSH levels were studied.Results Serum levels of LH and FSH had no significant difference among group A,group B and the control group (P＞0.05).Serum level of LH and FSH was significantly higher in group C than that in group A,group B and the control group,and the differences had statistical significance (P＜0.05).Serum level of LH and FSH in group D had no significant difference compared with that of the control group (P＞0.05).Serum level of LH and FSH in group E was significantly higher than that in group D and the control group,and the difference had statistical significance (P＜0.05).The level of LH and FSH was significantly higher in abnormal testicular group than that in normal testis group,and the difference had statistical significance (P＜0.05).Spearman correlation analysis showed that the spermatogenic function of testis was negatively correlated with serum LH and FSH levels (r=-0.473,-0.441,P＜0.05).Testicular volume was negatively correlated with serum LH and FSH levels(r=-0.643,-0.715,P＜0.05),and the difference had statistical significance.Conclusion The determination of serum LH and FSH levels has important clinical value for predicting testicular spermatogenic function in azoospermia patients,and can be used to guide clinical puncture and prognosis judgement.

Objective To study the causes, technique, selection and results of revision surgery in the patients with cervical spondylosis after anterior decompression. Methods Twenty nine patients with cervical spondylosis were treated with revision surgery after anterior decompression. Among them, there were 19 patients with cervical myelopathy, 8 with combination of myelopathy and radiculopathy, and 2 with radiculopathy only. The procedures included removal of the residual compressive elements, migrated bone grafts and implants, and refusion with bone graft and internal fixation. Depending on the clinical symptoms and findings of physical examination, X ray films and MRI were used to analyze the causes and the results of the revision surgery. Results There were 11 patients with incomplete initial decompression, 4 patients with the migration of the bone graft, 4 patients with new neural compression after cages interbody fusion, 2 patients with loose and migration of the plates and 8 patients with the degenerative changes in adjacent segments. Solid fusion in 23 cases and delayed fusion in 6 cases were observed during follow up. The neural recovering rate after the revision surgery was 52.4% in the patients with cervical myelopathy and 46.7% in the patients with combination of myelopathy and radiculopathy. Conclusion The main causes for the reoperation after anterior decompression were residual or newly formed neural compression. The complete decompression and segmental stabilization in the cervical revision surgery was essential for obtaining the good results.

Objective To evaluate the diagnostic value of the nerve root sedimentation sign for lumbar spinal stenosis and improve morphological classification.Methods This is a retrospective radiologic study.From November 2012 to November 2013,511 patients who carried out MR examination due to backache together with intermittent claudication were selected.Cross-section MRI images at the narrowest layer of canalis spinalis were chosen.The patients were further divided into 7 groups,A1,A2,A3,A4,B,C,and D based on Schizas.The dural sac cross-sectional area (DSCA) of each group was measured.The groups without difference were merged.A new method to grade nerve root sedimentation was developed.Then,the correlation and consistency of nerve root sedimentation dichotomy,Schizas classification,new nerve root sedimentation classification and DSCA classification were compared.The differences of patients' Oswestry disability index (ODI) which adopted new classification method were compared.Results Results show there are no statistical significance between groups A1 and A2,groups A4 and B,or groups C and D in DSCA.After the merger,new nerve root sedimentation classification includes negative,a,b,and c.The correlation between nerve root sedimentation dichotomy and DSCA method is rs=0.579,and the consistency is κ=0.254.The correlation between new nerve root sedimentation classification method and DSCA method is rs=0.722,and the consistency is κ=0.381.The scores improve compared with the first two classification methods.ODI score difference of each group which adopted new nerve root sedimentation classification method has statistical signiftcance (F=17.972,P=0.000).Pairwise comparison shows only group c differs significantly from other groups (P＜0.05).Conclusion The new nerve root sedimentation classification method is more effective than nerve root sedimentation dichotomy and Schizas grading.It reflects the severity degree of patients'clinical symptoms to some extent.

Measuring the innovation capacity and competitive strength of the department mainly depends on the quality and quantity of its independent intellectual property.Here we discussed intellectual property issues occurring in the different stages of the application,approval,implementation and concluding,including the theses written at the application stage,the novelty retrieval of patent documentation,periodic achievement protection during implementation,the association between the patent application and paper publication,etc.And the intellectual property ownership was expounded according to Contract Law of PR China.The strategy of intellectual property protection was put forward from the view points of the mechanism,regulation,and personnel.The helpful tips are expected to the scientific researcher and the administrative staff.

Objective To retrospectively analyze the clinical classification ,laboratory and imageological examinations of Wil‐son′s disease for strengthening the cognition on this disease and increasing the diagnosis rate .Methods The clinical data of the pa‐tients with WD in our hospital from January 2011 to September 2014 ,were collected and performed the statistical analysis .Results Among different ages ,nationalities and genders of WD patients ,first onset age ,clinical classification and laboratory examination had some difference ,the average age of first onset in male WD patients was earlier than that in female WD patients ,the low density shadow change of nervous type WD existed in MRI .Conclusion Heredity is not a unique factor affecting WD ,gender ,age and na‐tionality may be one of factors affecting the first onset age and clinical symptoms in the WD patients .

OBJECTIVETo observe the improvements in constipation differentiated as intestine system syndrome and lung system syndrome treated with electroacupuncture (EA) and explore their correlation.

METHODSSeventy cases of severe functional constipation were randomized into an EA group and a pseudo-EA group, 35 cases in each one. In the EA group, the needles were inserted deeply at Tianshu (ST 25) and Fujie (SP 14) on bilateral sides, and directly went to parietal peritoneum. EA was attached to the needles, dense-disperse wave, 2 Hz/15 Hz, and 0.1 to 1.0 mA. The perpendicular insertion was done at Shangjuxu (ST 37), 25 mm in depth. After qi arrival, the needle was lifted, thrusted and rotated once every 10 min, for 3 times totally. The needles were retained for 30 min. In the pseudo-EA group, the pseudo-points lateral to Tianshu (ST 25), Fujie (SP 14) and Shangjuxu (ST 37) on bilateral sides were punctured shallowly. The electric stimulation was pretended to connect but with the electric wire cutting off. The needles were retained for 30 min. The treatment was given 5 times weekly in the first two weeks and 3 times weekly in the later 6 weeks. Totally, 28 treatments were required. TCM intestine and lung syndrome scale was used for evaluation. The changes in TCM syndromes were observed before and after treatment in the patients of the two groups.

RESULTSThe total score of intestine and lung syndrome and the score of individual syndrome were all reduced after treatment as compared with those before treatment in the two groups (both P<0.01). The improvements of the EA group in the total score of intestine system syndrome, the scores of large. intestine syndrome and stomach syndrome, the total score of lung system syndrome and the score of lung dysfunction in dispersing and descending syndrome were superior to those of the pseudo-EA group (P<0.01, P<0.05). The differences in the scores of lung qi deficiency syndrome and throat syndrome were not significant between the two groups (all P>0.05). Simultaneously, the very strong positive correlation (P<0.01) and positive linear correlation (P<0.01) were presented in the total score of intestine and lung syndrome of the two groups.

CONCLUSIONEA obviously improves in intestine system syndrome (including large intestine syndrome and stomach syndrome), as well as lung system syndrome (lung dysfunction in dispersing and descending) in the treatment of constipation. But the improvements are not apparent in the treatment of lung qi deficiency and throat disorder. Additionally, the obvious correlation is displayed in the improvements in intestine and lung syndrome.

Acupuncture Points ; Adult ; Aged ; Constipation ; physiopathology ; therapy ; Electroacupuncture ; Female ; Humans ; Intestines ; physiopathology ; Lung ; physiopathology ; Male ; Middle Aged ; Young Adult