Objective To research the effect of nimodipine in the prevention and treatment of delayed facial palsy after microvas-cular decompression .Methods A retrospective analysis was taken from January 2009 to March 2012 ,193 cases of patients with fa-cial spasm undergoing MVD in our department .According to whether applied vasodilators after operation ,all cases were divided into two groups :the experimental group(72 cases)with the treatment of nimodipine for two weeks ;the control group(121 cases) ,and not treated with nimodipine .The follow-up time was 6-12 months .Observe and compare the incidence ,level of delayed facial paraly-sis onset time and disease duration time between two groups .Results In the experimental group had 6 cases of late facial paralysis , the incidence was 8 .3% (6/72) ,onset time was (14 .5 ± 5 .2)days ,disease duration was (41 .3 ± 14 .4)days .The incidence of control group was 9 .1% (11/121) ,onset time was (12 .2 ± 7 .4)days ,disease duration was (55 .7 ± 36 .4)days .There was no significant difference between groups(P>0 .05) .According to House-Brackman classification ,there were 4 cases of grade Ⅱ and 2 cases of grade Ⅲ in experimental group .In control group ,there were 2 cases of grade Ⅱ ,6 cases of grade Ⅲ ,3 cases of grade Ⅳ .The differ-ence was statistically significant (P<0 .05) .Conclusion The delayed facial paralysis is influenced by a variety of causes .Vasodila-tor drugs can reduce the incidence of delayed facial paralysis ,and play a certain role in promoting facial paralysis restored .

AIM:To compare the effect of recombinant human epidermal growth factor eye drops and deproteinized calf blood extract eye drops on corneal edema after phacoemulsification.METHODS:Totally 72 cases (72 eyes) of patients undergoing phacoemulsification were selected and divided into the observation group and the control group by random number table method.After surgery,the observation group were treated with deproteinized calf blood extract eye drops while the control group were treated with recombinant human epidermal growth factor eye drops.The degree of corneal edema,subjective symptom score,corneal endothelium count,changes of corneal thickness and postoperative visual acuity recovery were compared between the two groups at different time points after surgery.RESULTS:Corneal edema in the two groups was significantly milder at 1wk after surgery than that on the 1st day after surgery (P<0.001).There was no significant difference in the degree of corneal edema and test results of visual acuity between the two groups at 1wk after surgery (P>0.05).Compared with 1d after surgery,the subjective symptom score and corneal thickness of the two groups significantly decreased on the 7th day after surgery (P<0.05).At 7d after surgery,the above indexes were significantly better in the observation group than the control group(P<0.05).At 1mo after surgery,the corneal endothelium count was significantly larger in the observation group than the control group (P<0.001).CONCLUSION:Both of recombinant human epidermal growth factor eye drops and deproteinized calf blood extract eye drops can significantly relieve corneal edema and improve visual acuity of patients after phacoemulsification.However,the latter has obvious advantages over the former in the repair of corneal endothelial cell injury after surgery.

Objective:To establish the experimental nude mouse model of endometriosis for human eutopic endometrium.Methods:Female nude mouse aged 6~8 weeks were selected.Nude mouse in experimental group(n=20)were implanted with the endometrium of patients with endometriosis or without endometriosis into pelvic and abdominal cavities.Nude mouse in control group(n=3)were implanted with the greater omentum.Nude mouse were killed at 5th,15th,and 30th day after implantation for observ ation of the growth of endometriotic lesions.The morphological and histological changes of endometriotic lesions from diferent sources and at diferent time points of growth were examined by light microscopy.Results:The achievement ratio of transplantation was 100%.At the 5th day,endometriotic lesions were found in the pelvic and abdominal cavities,and the adhesion to mice tissues was already tight.There were nascent vascular net between endometriotic lesions and mice mesepithelium.Endometrial glands around with stroma cells were found under light microscope.At the 15th day,endometriotic lesions mixed with mice tissues.Endometriotic lesions presented parenchymatous and cystiform.A lot of endometrial glands and stroma cells with rich blood supply were seen under light microscope.At the 30th day,endometriotic lesions became atrophied,and the adhension was serve partly.Endometriotic lesions presented severe fibrosis and some endometrial cells were not in integrity under light microscope.In the control group,some of the greater omentum adhered to the incisal opening of abdominal wall,while the others were free and atrophied or merged gradually.There was no change in the constructed greater omentum except for some inflammatory reaction.Conclusions:Nude mouse model of endometriosis was good for studying the endometria cell proliferation,neovascularization,hormone,immune response and so on,since the implanted endometrium remain the original morphological and histological features.It is a perfect model of endometriosis because of its significant and steady character,low cost,simple operation and short observation cycle.

This paper was to explore the application of case based learning method in medical psychology course for medical undergraduates.According to curriculum standard,nine cases were compiled which matched six articles,forming the complete instructional design.We followed the five steps of scientific teaching stage.The preliminary results showed that the case teaching was helpful to improving the medical students' knowledge acquisition,and their ability to master and solve practical problems.

Objective:To establish a method for the separation and purification of haemocoagulase from agkistrodon blomhoffii in Changbai Mountain. Methods: An enzyme component with clotting activity was isolated from the venin of agkistrodon blomhoffii in Changbai Mountain by gel filtration, anion-exchange chromatography and heparin-Sepharose affinity chromatography. SDS-Page and RP-HPLC were used to determine its purity, SDS-Page was applied to study its action mode with bovine fibrinogen, HPSEC was used to determine the molecular weight, an IEF method was employed to detect its isoelectric point, and Lowry method was used for the deter-mination of protein concentration. Results:One haemocoagulase was purified from agkistrodon in Changbai Mountain. SDS-Page dis-played one band, and HPLC showed one single chromatographic peak. The haemocoagulase acted only on α chain of fibrinogen. Its molecular weight was 32. 2kD with isoelectric point of 5. 21. The enzyme had clotting activity in vitro. Conclusion:The method can be used for the separation and purification of haemocoagulase from agkistrodon blomhoffii in Changbai Mountain.

Objective To evaluate the safety and effectiveness of robotic surgery in surgical staging of endometrial cancer. Methods Searched English and Chinese databases, including Cochrane library, PubMed, Embase, Web of Science, China National Knowledge Internet, data base of Wanfang, China Science and Technology Journal(CSTJ), and relevant journals and magazines by hand from Jan. 2000 to Oct. 2016.(1)In accordance with the inclusion criteria,two independent investigators screened databases and extracted the relevant data respectively, then evaluated the quality of including studies in Newcastle-Ottawa Scale(NOS).(2)Meta-analysis was performed with RevMan 5.3 software. Heterogeneity inspection was done for each study and different effect model included the random effect model and fixed effect model was chose according to the results of the inspection. At last, the related parameters of the robotic surgery and laparoscopic surgery was analysed. Results (1) Thirteen articles were ultimately included. All of them were written in English and included a total of 1554 patients, included 739 cases of robotic surgery and 815 cases of laparoscopic surgery. Thirteen articles were all cohort study, four of them were prospective cohort study, while others were retrospective cohort study. After quality assessment, all studies had more than 5 stars and illustrated the higher quality.(2)Meta-analysis results showed:compared with laparoscopic surgery in surgical staging of endometrial cancer, robotic surgery had less estimated blood loss [standard deviation (SD)=-72.31 ml,95%CI:-107.29 to-37.33,P<0.01], less time for hospital stay (SD=-0.29 days,95%CI:-0.46 to-0.13,P=0.001), less need for blood transfusion [risk ratio (RR)=0.57,95%CI:0.33 to 0.97,P=0.040], and conversion to open surgery (RR=0.41,95%CI: 0.26 to 0.65,P=0.000),less intraoperative complications (RR=0.43,95%CI: 0.24 to 0.76,P=0.004) in surgical staging of endometrial cancer. There was no statistically significant difference in aspects of operative time (SD=10.26 minutes, 95%CI:-13.62 to 34.13,P=0.400), postoperative complications (RR=0.87, 95%CI: 0.67 to 1.12,P=0.280), the total number of lymph nodes removed (SD=-0.04,95%CI:-3.99 to 3.91,P=0.980), the number of pelvic lymph node dissection (SD=0.48,95%CI:-1.76 to 2.71,P=0.680) and the number of para-aortic lymph node dissection (SD=0.46,95%CI:-1.42 to 2.34,P=0.630). Conclusions Compared the robotic surgery with laparoscopic surgery in surgical staging of endometrial cancer, robotic surgery has less estimated blood loss, less need for blood transfusion and conversion to open surgery,less intraoperative complications and other advantages. While its cost is so expensive that restrict clinical application.

Objective To deterine the expression of TNF-related apoptosis inducing ligand (TRAIL) and its receptor DR5 in murine myocardium with acute viral myocarditis(VM).Methods We builted the model of VM.Eight mice of the VM group and the normal control group were sacrificed on the 7th,10th,14th,21st,28th day after inoculation CVB3 virus.The myocardial histopathological scores were counted.The terminal reansferase-mediated dUPT-biotin nick end-labeling (TUNEL) assays was used to quantified apoptosis rate.The expression of TRAIL and DR5 protein and mRNA were determined by the method of immunohistochemistry and reverse-transcription polymerase chain reaction (RT-PCR).Results The expression of TRAIL and DR5 protein and mRNA were found in myocardium of both the normal control group and the VM group.The expression of TRAIL protein of the VM group(14th) were significantly higher than which in the normal group at the same time and the VM group(7th)(F=9.17,P<0.01).The DR5 protein of the VM group(10th,14th,21st)were significantly higher than which in the normal group at the same time and the VM group(7th)(F=13.32,P<0.01).The expression of TRAIL and DR5 protein were positive correlated with the myocardial histopathological scores and the apoptosis rate.The expression of TRAIL mRNA of the VM group(10th) were significantly higher than which in the normal group at the same time and the VM group(7th)(F=10.86,P<0.01).The DR5 mRNA of the VM group(10th,14th)were significantly higher than which in the normal group at the same time and the VM group(7th)(F=22.75,P<0.01).Conclusion High characteristic expressions of protein/mRNA TRAIL and DR5 were observed in the myocardium of mice with VM.The level was positive correlationed with the account of pathology and the rate of apoptosis.The apoptosis induced by TRAIL and DR5 may participate in the pathophysiological processes of VM.

Chronic kidney disease (CKD) has become a serious impact on global human public health, one major problem with high morbidity,low awareness, high cost of treatment, as well as the high mortality rate. In recent years, both at domestic and foreign have been carried out large-scale epidemiological study of CKD screening and in the incidence of CKD, detection methods and risk factors, etc. With a better understanding. In this paper the definition of CKD and staging, morbidity, detection methods, risk factors and causes of such areas as research progress v. Comprehensive guidance and services in clinical work.

OBJECTIVE: To establish an HPLC method for the determination of 8-O-acetyl-shanzhiside methylester in Tibet medicinal Lamiophlomis rotata.METHODS: HPLC analysis was performed on Symmetry C18(150 mm?4.6 mm,5 ?m) column with the mixture of acetonitrile-water(15∶85) served as the mobile phase.The detection wavelength was set at 234 nm.RESULTS:The linear range of 8-O-acetyl-shanzhiside methylester was 1.97～19.68 ?g?mL-1(r=0.999 6) and its average recovery was 99.53%(RSD=1.73%,n=9).CONCLUSION: The method is simple,accurate and specific,and it applies reference for quality evaluation and utilization of Tibet medicinal L.rotata.

The time-dependent changes in myocardio membrane system during ischemia/reperfusion injury were studied with ligature of the left ventricular branch of coronary artery in rabbit. The permeability of cardiac membrane system being tested by using lanthanum tracer technique and stereological methods. Lanthanum granules were observed in the sarplacosma and sarccpiasmic tubes especially at ischemia for 20min. The pecmeability of membrane system for La~(+++) was more marked when hearts were reperfused for 20min. With the increase in time of ischemia, the changes became more and more serious. A lot of lanthanum granules were entered into mitochondria when the hearts were reperfused after 40min ischemia. There were neither intact mitichondria nor lanthanum granules in mitochondria observed when the hearts were reperfused after 60min ischemia. The structural changes were irreversible at this stage of reperfusion The results of our study indicated that the changes of permeability of cardiac cell for La~(+++) in ischcmia/reperfusion are time-dependent, 40min ischemia is the demarcation line between the reversible and irreversible injury during reperfusion. On the basis of our experiment, we like to propose a new conception of reversible and irreversible injury of reperfusion.