1.Quality standard of processed products of Rhizoma Atractylodis Macrocephalae
Yongming YU ; Changyi SONG ; Tianzhu JIA
Chinese Traditional Patent Medicine 1992;0(06):-
AIM: To research the processed products of Rhizoma Atractylodis Macrocephalae at the level of sesquiterpene lactones from it. METHODS: Sesquiterpene lactones were separated by the silica gel column and layer preparation, and the active substances were determined by HPLC. RESULTS: Atractylone、atractylolide Ⅰ, atractylolide Ⅲ, and biepiasterolid were separated from Rhizoma Atractylodis Macrocephalae. Atractylone was (0.535 8)%, atractylolide Ⅰ was (0.044 0)% and actractylolide Ⅲ was (0.081 4)% in Atractylodis stir-fried with wheat bran determined by HPLC. CONCLUSION: The method has good accuracy and repeatability and it can be used for the quality control of Rhizoma Atractylodis Macrocephalae.
3.Isolation and activity of an α-amylase inhibitor from white kidney beans
Xiaoqi ZHANG ; Mingyan YANG ; Yu MA ; Jia TIAN ; Jirong SONG
Acta Pharmaceutica Sinica 2007;42(12):1282-1287
An α-amylase inhibitor (α-AI) was isolated from white kidney beans (Phaseolus vulgaris. L) by ethanol fractional precipitation, ion exchange chromatography and gel filtration column chromatography. It was a homogeneity glycoprotein demonstrated by SDS-PAGE and gel filtration on CL-6B. The glycoprotein contained 88.2% protein and was rich in aspartic acid, glutamic acid, leucine, threonine and serine. The carbohydrate moiety was consisted of Man, Glc, Gal and Xyl in a mole ratio of 2.42∶1.50∶1.52∶1.00. The glycan and the core protein backbone was connected by O-linkage as determined by β-elimination reaction. The continuous oral administration of the α-AI (150 mg·kg-1·d-1 ) for 7 days can lower fasting blood glucose and 300 mg·kg-1 ·d-1 α-AI for 7 days can improve the sugar tolerance on alloxan-dependent diabetic model rats. The result showed the α-AI obtained from white kidney beans had good hypoglycemic effect on alloxan induced diabetic rats and may have high potential pharmaceutical value as a regulative digestive-starch degradation in patients suffering from diabetes.
4.The cut-point for glycosylated hemoglobin in different populations in the plateau region
Ya LI ; Demei JIA ; Ying ZHAO ; Zijie LIU ; Yu SONG ; Dianping SONG ; Yong DUAN
Chinese Journal of Laboratory Medicine 2013;(2):142-145
Objective To explore the optimal HbAlc diagnostic cutpoint in different glucose tolerance populations in the plateau region.Methods (1) 472 diabetes mellitus (DM) patients and highrisk groups accepting diabetes screening in the First Affiliated Hospital of Kunming Medical College (217 males and 255 females,≥20 years old,median age 54 years old) were collected,oral glucose tolerance test (OGTT) and HbAlc were tested.(2) the research subjects were divided into normal glucose adjustment group (NGT),Impaired fasting glucose group (IFG) and (or) Impaired glucose tolerance IGT group and diabetes mellitus (DM) group.The receiver-operating characteristic curve (ROC) was explored to determine the optimal HbA1c diagnostic cut point for IFG,IGT and DM status respectively.Results The average HbA1 c values of NGT,IFG and (or) IGT,DM groups were (6.06 ± 0.11) %,(6.63 ± 0.11) %,(8.70 ± 2.08)% respectively,for IFG and IGT groups,the optimal HbA1c diagnostic cut points were 6.7% and 6.6%,respectively; If use either FBG or 2 h PG to diagnose DM,the corresponding optimal HbA1 c diagnostic cut point was 7.1% ; If use anyone of FBG or 2hPG to diagnose DM,the corresponding optimal HbA1c diagnostic cut point was 7.0% ; If both FBG and 2hPG were used to diagnose DM,the corresponding optimal HbA1 c diagnostic cut point was 7.1%.Conclusion Preliminarily confirm the optimal HbA1c diagnostic cut point in different glucose tolerance populations in the plateau region of Kunming,and provide the evidence for further clinical application of HbA1c.
5.Synthesis and anti-SARS-CoV-2 activity and mechanism research of lycorine derivatives
Yu-heng MEI ; Jia-yu LI ; Dan-qing SONG ; Zong-gen PENG ; Ying-hong LI
Acta Pharmaceutica Sinica 2024;58(2):395-403
We designed and synthesized eighteen lycorine derivatives with five different structural types, and evaluated their antiviral activities on a HCoV-OC43-infected H460 cell model. Structure-activity relationships suggested that the introduction of appropriate substituents on the 6N atom of lycorine was beneficial to activity. Compound
6.Antagonistic effect of baicalin on oxidative stress injury in neurons and astrocytes of rats.
Yuan-zong SONG ; Yu-jia YANG ; Yan-jie JIA
Chinese Journal of Integrated Traditional and Western Medicine 2004;24(4):339-342
OBJECTIVETo explore the experimental conditions for H2O2 to injure astrocytes and the effect of baicalin in protecting neurons and astrocytes from oxidative stress injury.
METHODSNeurons and astrocytes from forebrain of rats were cultured in vitro and treated with H2O2, baicalin and combination of the two, respectively. The cell viability or survival rate was determined using MTT.
RESULTSEffects of H2O2 in different concentrations on survival rate of astrocytes showed significant difference (F = 28.569, P < 0.01) in a dose-dependent manner. Degrees of H2O2 injury, with the same concentration of H2O2, on cells with different seeding density were also significantly different (F = 5.439, P < 0.01), and dose-dependently. Baicalin didn't influence the survival rate of neurons and astrocytes when the concentration was within 2.5-40 mumol/L (for neurons, F = 0.49, P > 0.05; for astrocytes, F = 1.001, P > 0.05), but baicalin showed significant antagonism to the injury of oxidative stress (for neurons, F = 24.384, P < 0.01; for astrocytes, F = 5.000, P < 0.01). The higher the concentration of bainalin, the higher the cell survival rate.
CONCLUSIONA model of astrocytes oxidative injury induced by H2O2 is established. Baicalin shows no toxicity on neurons and astrocytes when the concentration is within 2.5-40 mumol/L, but could antagonize the H2O2 caused oxidative injury on cells in a dose-dependent manner.
Animals ; Animals, Newborn ; Astrocytes ; pathology ; Cells, Cultured ; Flavonoids ; pharmacology ; Hydrogen Peroxide ; Neurons ; pathology ; Neuroprotective Agents ; pharmacology ; Oxidative Stress ; Rats ; Rats, Sprague-Dawley
7.Effects of Wenjing Tongluo Formula on GFAP Level in Perpheral Neuropathy Rats
Yuanyuan WANG ; Liqun JIA ; Bo DENG ; Honglei ZHANG ; Aiping SONG ; Jiping DA ; Lili YU
Chinese Journal of Information on Traditional Chinese Medicine 2015;(4):70-73
Objective To discuss the treatment action and mechanism of Wenjing Tongluo Formula on oxaliplatin-induced perpheral neurotoxicity in rats. Methods Intraperitoneal injection was used to inject oxaliplatin 4 mg/kg to establish oxaliplatin-induced peripheral neurotoxicity rat models. Female Wistar rats were randomly divided into normal group, model group, and TCM group. TCM group was given Wenjing Tongluo Formula to soak rats’ limbs and tails. Rats in the model group were soaked with deionized water for comparison. Rats in the normal group received intraperitoneal injection with 5%glucose. Algesia hypersensitivity and anaphylaxis were detected under the mechanical stimulation and temperature. Immunohistochemical method was used to detect the expression of GFAP in L4-L6 spinal dorsal horn of rats. Explore the level of GLT-1 in L4-L6 dorsal root ganglia by RT-PCR. Results Rats in model group showed obvious behavioral changes compared with normal group (P<0.05);Rats in the TCM group improved in behavioristics compared with model group (P<0.01);number of positive cells in GFAP of rats in the model group increased compared with normal group (P<0.05);the increase in the TCM group was not obvious. Compared with normal group, astrocytes in spinal dorsal horn of model group were enlarged, protuberances increased, became coarse, and GLT-1 mRNA is decreased (P<0.05, P<0.01). Compared with model group, active cells and protuberances in the TCM group decreased (P<0.01), GLT-1 mRNA is increased (P<0.01). Conclusion Wenjing Tongluo Formula can improve behavioral changes of model rats under temperature and mechanical stimulation, probably related to harmful signal transmission induced by inhibition of astrocyte in spinal dorsal horn.
8.The relationship of regular exercise and coronary collateral of patients with acute coronary syndrome
Pengli XI ; Yunqiang ZHANG ; Mu GUO ; Zhi JIA ; Haiqing LIANG ; Yu SONG
Clinical Medicine of China 2014;30(2):127-131
Objective To investigate the relationship between regular exercise habit and coronary collaterals of patients with acute coronary syndrome (ACS).Methods TWo hundred and thirty-night patients diagnosed ACS and operated coronary angiography (CAG) showing severe coronary stenosis were enrolled hospitalized from May 2012 to October 2012.They were divided into regular exercise group (n =102) and irregular exercise group(n =137) according to the exercise frequency.The information of the general data,the information of CAG and other relevant index were collected.The coronary artery score was recorded according to the Censini and the coronary collateral class was made according to the Rentrop.Other characters in clinical and laboratory were recorded.Multi-factor regression analysis was used to analysis the influence factors of coronary collateral.Results The proportion of coronary collaterals (41.2% (42/102)) in the regular exercise group was higher than that in the irregular exercise group (24.1% (33/137)),and the difference was statistically significant(x2 =7.929,P =0.005).Lg(Gensini score) was (1.89 ± 0.18) the and (1.94 ± 0.19) in the regular exercise group,The left ventricular ejection fraction was 57.0% (52.0%,60.0%) in the regular exercise group and 50.0% (45.0%,57.0%) in the irregular exercise group,and the difference was statistically significant (Z =-5.152,P =0.000).Multi-factor regression analysis showed that regular exercise (OR =3.423,95% CI:1.790-4.578),diabetes mellitus (OR =0.451,95% CI:0.212-0.962),B-type natriuretic peptide (OR =2.412,95 % CI:1.271-4.578),non-ST-segment elevation ACS (OR =2.383,95% CI:1.185-4.791),chest pain history (OR =2.207,95% CI:1.175-4.145),Gensini score (OR =1.538,95% CI:1.141-2.073) were independent influence factors of coronary collateral(P < 0.05).After adjusting other factors,the patients with regular exercise had better coronary collaterals than that with irregular exercise (OR=3.423,95%CI:1.790-6.548,P <0.001).Conclusion The regular exercise can promote coronary collateral emergence for the patients with ACS.
9.Efficacy of levosimendan on cardiac function of patients with decompensated heart failure
Zhi JIA ; Mu GUO ; Liyuan ZHANG ; Yunqiang ZHANG ; Haiqing LIANG ; Shuguang TIAN ; Yu SONG
Clinical Medicine of China 2014;30(2):113-118
Objective To evaluate the improvement effect of levosimendan by vein injection on short term cardiac function of patients with decompensated heart failure.Methods One hundred and sixty patients admitted due to heart failure were randomly divided into levosimendan group and control group (80 subjects for each group).Patients in control group were given a regular therapy including diuretics,vasodilators (including the recombinant human brain natriuretic peptide),angiotensin converting enzyme inhibitor(ACEI) or angiotensin Ⅱ receptorantagonists(ARB),β blockers,spironolactone and stain.Patients in levosimendan were administered levosimendan for 24 hours plus regular therapy.The improvements of dyspnoea in 9 days and cardiac function classification in 30 days after therapy were assessed.Mortality of 1 month and 3 month in two group were calculated and compared during follow-up.Results The dyspnoea improvement rate was superior than that of control group during 9 days (OR =1.956,95% CI:1.156-3.310,P =0.013).The improvements in the levosimendan group were better than in the control group at 1 st day (OR =2.261,95 % CI:1.280-3.999,P =0.005),at 3rd (OR =2.002,95 % CI:1.111-3.607,P =0.021) and 5th day (OR =1.846,95 % CI:1.009 -3.377,P =0.047).However,there was no significant difference in term of improving dyspnoea between the levosimendan group and the control group at 9th day (P =0.126).Similarly,the improvement of cardiac function classification in the levosimendan group was superior than the control group during 30 days (OR =1.933,95% CI:1.229-3.040,P =0.004).Although no significant difference was seen regarding of improving cardiac function classification between the two groups at 30th day after treatment (P =0.115),the improvements in the levosimendan group were better than in the control group at 3rd (OR =1.986,95% CI:1.195-3.300,P =0.008),5th (OR =2.268,95 % CI:1.329-3.873,P =0.003),9th (OR =2.627,95 % CI:1.419-4.860,P =0.002) and 14th day(OR =2.212,95% CI:1.189-4.112,P =0.012).Moreover,there was a nonsignificant reduction in terms of mortality in levosimendan group during 1-month and 3-month follow-up compared with control group (P > 0.05).Condusion Levosimendan can effectively improve the short-term cardiac function in patients with decompensated heart failure.
10.Mutation of the Strain Producing Higher Xylanase
Hong-Ge CHEN ; Xin-Yu LIU ; Shi-Min ZHANG ; An-Dong SONG ; Xin-Cheng JIA ;
Microbiology 1992;0(06):-
A.niger M1, the initial strain, was treated by UV and a mutant with 30% higher xylanase activity was obtained. Zymogram for detecting xylanase showed there are three different xylanases in the mutant mature culture, while two xylanases in initial strain. After orthogonal experiment, the optimum fermentation conditions of the mutant were obtained as follows: concentration of the major carbon resource 4 %, ratio between bran and corncob 5:5, concentration of glucose 0.1%, concentration of ammonium oxalate as supplemental nitrogen resource 2.0%, the initial pH of liquid medium 5.0, 100mL/250mL flask.