1.Status of iodine content of common foods in different geographical areas in Fujian Province
Zhi-hui, CHEN ; Yu-gong, ZHAO ; Jia-ni, WU
Chinese Journal of Endemiology 2013;(3):276-280
Objective To determine the iodine content of common foods in different geographical areas (coastal city,coastal rural area,mountainous city and rural area) in Fujian Province and provide basic data for evaluation of dietary iodine intake.Methods In 2010,based on the types of food of the total diet study,one food sample(consumption rate is greater than 1%) was collected in coastal city(Taijiang),coastal rural area(Xiang'an),mountainous city (Sanyuan) and rural area(Mingxi).These foods including cereal,beans,potato,meat,eggs,milk,aquatic products,vegetables,fruits,sugar,beverages,liquor and seasoning,and so on 184 kinds of common foods.The iodine content of these food was tested by As (Ⅲ)-Ce4+ catalytic spectrophotometry.Results Among the 184 kinds of food tested,164 were not indicated food iodine content in the Chinese Food Composition Tables (2004).The iodine content of common food in Fujian Province in descending order were salt (30 000 μg/kg),aquatic products(341.4 μg/kg),eggs(255.9 μg/kg),dairy(106.7 μg/kg),meat(103.2 μg/kg),cereals (40.7 μg/kg),vegetables(30.7 μg/kg),beans(12.9 μg/kg),sugar(8.5 μg/kg),fruits(6.7 μg/kg),potatoes(2.4 μg/kg) and alcohol(2.1 μg/kg).The iodine content of kelp and laver was the highest in all the food,which was 314 780.1,176 956.5 μg/kg,respectively.The iodine content of food from animal(241.4 μg/kg) was much higher than that of the food from plant(25.4 μg/kg).The iodine content of common cereals,potatoes,beans,sea algae,meat,eggs and aquatic products was compared in the four areas,and the difference was not statistically significant (M =135.5,20.0,42.0,16.0,54.0,4.0,x2 =1.4,all P > 0.05).The iodine content of vegetables and fruits was compared,and the difference was statistically significant (x2 =8.5,M =204.5,all P < 0.05).Conclusion The iodine content of different foods is different.
2.Expression of osteopontin in renal tissue of rats with endemic fluorosis caused by exposure to coal burning and with low calcium
Ji-can, LIU ; Jia-qi, WANG ; Yan-ni, YU
Chinese Journal of Endemiology 2010;29(4):379-383
Objective To detect the osteopontin(OPN)expression in renal tissue of rats with fluorosis and low calcium diet,and study the role of OPN in renal injury of fluorosis.Methods Forty-eight aged 1 month Wistar rats,80-120 g,were randomly divided into 4 groups by 2×2 factorial design(the number of female and male in each group was equal):the control group,high-flluoride group,low-calcium group and low-calcium with high-fluoride group.All rats of the fluorosis groups were fed with feed containing corn exposed to coal-burning from endemic fluorosis areas with high fluoride(100 mg/kg,corn),the other two groups were fed with feed containing coru from nonendemic fluorosis areas(fluoride 5 mg/kg,corn).After 16 weeks,the rats were killed.The change of teeth was examined,and the incidence rate of dental fluorosis was calculated.The expressions of both protein and mRNA of OPN in rat renal tissue were determined by RT-PCR and immunohistochemistry after four-month experimentation.Results The growth of teeth was very well in the control group and the low-calcium group.The two high-fluoride groups showed evident dental fluorosis(100%).The results of immunohistochemistry showed that the OPN protein was localized in renal tubule cytoplasm.The OPN-positive cells from renal tissue were lightly and scatteredly stained in control and low-calcium groups.The OPN-positive cells had deeper color in high-fluoride group and low-calcium with high-fluoride group,widely distributed in the renal tubular epithelial cells.The protein expression of OPN in the two groups exposed to fluoride(168.64±13.21,169.26±8.92)was significantly higher than those of the corresponding control group(145.78±10.26,all P<0.01)and low-calcium group(149.60±16.84,all P<0.01).The mRNA expression of OPN in the two groups exposed to fluoride(1.89±0.37,1.94±0.22)was significantly higher than those of the corresponding control group(1.32±0.26,all P<0.05)and low-calcium group(1.30±0.186,P<0.05),respectively.High fluoride influenced the expression of protein and mRNA of OPN(F=13.821,4.24,all P<0.05).Low calcium did not affect the expression of protein and mRNA of OPN(F=2.164,0.58,all P>0.05).However,high fluoride and low calcium had a cross interaction on the expression of protein and mRNA of OPN(F=6.257,432,all P<0.05).Conclusions Over-dose fluoride enhances the expression of OPN.The higher expression observed in the cases exposed to high fluoride concentration is associated with serious renal injury.OPN may he a potential marker for renal injury in fluorosis.Moreover,over-dose fluoride and low calcium make the renal injury worse,indicating low calcium plays an important part in renal injury by fluoride.
3.The use of dental papillae cells and millipore filter for bioengineered dentin.
Wei-jia NI ; Yu-cheng LI ; Jia-yuan WU ; Long-xing NI
Chinese Journal of Stomatology 2010;45(11):678-683
OBJECTIVETo observe the ability of SD rat dental papillae cells forming dentin-like structure induced by millipore filter combined with transforming growth factor-β(1) (TGF-β(1)).
METHODSThe first passage SD rat dental papillae cells were enzymatically dissociated and centrifuged to obtain a cell mass. The cell mass was seeded on the millipore filter combined with TGF-β(1). The complex was incubated for 6 d in vitro or transplanted under the renal capsule for 2 weeks. Then the differentiation of dental papillae cells on the filter and the formation of mineral tissue on the implant were analyzed.
RESULTSA layer of polarized columnar cells were observed along the surface of the millipore filter, with cell processes extending into the porous media. Dentin sialoprotein (DSP) and dentin matrix protein-1 (DMP-1) were positive in these cells. After 2 weeks, tubular dentin matrix was deposited on the surface of the aligned cells. Scanning electron microscopy showed that the thickness of newly formed tubular dentin was consistent. DSP and DMP-1 were expressed in columnar cells, tubular matrix and the dental papillae cells adjacent to the filter.
CONCLUSIONSThe millipore filter combined with TGF-β(1) could effectively recruit progenitors onto its surface and induce odontoblast differentiation, secrete matrix in a homogenous manner, leading to dentinogenesis.
Animals ; Cell Differentiation ; Cells, Cultured ; Dental Papilla ; cytology ; Dentin ; Dentinogenesis ; Extracellular Matrix ; Extracellular Matrix Proteins ; Micropore Filters ; Odontoblasts ; Phosphoproteins ; Rats ; Sialoglycoproteins ; Tissue Engineering ; Transforming Growth Factor beta ; Transforming Growth Factor beta1 ; pharmacology
4.Angiomyolipoma of the kidney with lymph node involvement.
Chuan-Zhen WU ; Feng-Hua WANG ; Cheng-Mei LI ; Wen-Chang FANG ; Jia-Ni YU
Chinese Journal of Pathology 2005;34(11):715-715
Adrenalectomy
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Adult
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Angiomyolipoma
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pathology
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surgery
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Follow-Up Studies
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Humans
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Kidney
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pathology
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Kidney Neoplasms
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pathology
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surgery
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Lymph Node Excision
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Lymph Nodes
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pathology
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Lymphatic Metastasis
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Male
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Nephrectomy
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Ureter
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surgery
5.Association of red blood cell damage with arachidonic acid.
Tao YUAN ; Jian-ning ZHAO ; Jia MENG ; Yu CONG ; Shuang-shuang CHEN ; Ni-rong BAO
China Journal of Orthopaedics and Traumatology 2016;29(2):179-183
OBJECTIVETo study the correlation between arachidonic acid (AA) and acute red blood cells damage in rats, and to build a model with hidden blood loss in vivo, and to explore the pathological mechenism of hidden blood loss.
METHODSA total of 50 male adult Sprague-Dawley rats weighing (200 ± 20) g were randomly divided into five groups (n = 10): control group and four experimental groups. The rats in the experimental groups were given 0.5 ml different concentrations of AA dilu- ents, 5, 10, 20, 40 mmol/L respectively. The blood samples were collected from orbital venous at the beginning and 24, 48, 72 hours after administration. Then the changes of hemoglobin (Hb) ,red blood cell count (RBC), glutathione peroxidase (GSH- PX) activity, total superoxide dismutase (T-SOD) activity and hydrogen peroxide (H202) in the blood samples were tested.
RESULTSSignificant hidden blood loss occurred when the concentration was 10 mmol/L in the experimental group, with the RBC and Hb sharply reduced in blood samples. The Hb and RBC were reduced in all the experimental groups and control group at 24 hours after administration, while in the experimental groups they changed more obviously. The GSH-PX activity, T-SOD activity and H₂O₂were also significantly reduced in all groups, and the changes showed significant differences. The Hb and RBC were relatively stable in the control group and the experimental groups at 48 hours after administration; while GSH-PX activity, T-SOD activity and H₂O₂were all significantly decreased, and the changes in the experimental groups were more notable.
CONCLUSIONElevated levels of AA in the blood causes oxidative stress in the red blood cells, leading to the damage of red blood cells and hemoglobin, which is responsible for hidden blood loss.
Animals ; Arachidonic Acid ; toxicity ; Erythrocytes ; drug effects ; metabolism ; Glutathione Peroxidase ; blood ; Hemoglobins ; analysis ; Male ; Rats ; Rats, Sprague-Dawley ; Superoxide Dismutase ; blood
6.Analysis of monitoring results of patients with iodine deficiency disorders in Fujian Province in 2011
Jia-ni, WU ; Zhi-hui, CHEN ; Mu-hua, WANG ; Yu-gong, ZHAO
Chinese Journal of Endemiology 2013;32(4):404-407
Objective To analyze the monitoring results of patients with iodine deficiency disorders,and to find out the eliminating process of iodine deficiency disorders and the iodine nutritional status of population before adjustment of iodine level in edible salt in Fujian Province.Methods Thirty counties(cities,districts) were sampled by population proportion probability sampling in the whole Province in 2011; one primary school was selected from each of those 30 counties(cities,districts) ; 40 children aged 8-10 were selected from each of those 30 primary schools; thyroid volume of these children was examined by type-B ultrasound and the iodine level in edible salt from those 40 households was tested by the method of direct titration.Twelve urine samples of those 40 children were collected randomly,and urinary iodine level was tested by arsenic cerium catalytic spectrophotometry.Resident's per capita salt intake was calculated by the three-day-weighing method.In the village(where the school was in),5 drinking water samples were collected in the north,the south,the east,the west and the center of the village.If the water supply was centralized in the village,then 2 tap water samples were collected.Water iodine level was determined by arsenic cerium catalytic spectrophotometry.Three townships(towns,street offices)were selected in the vicinity of those schools; 5 pregnant and 5 lactating women were selected in each of those 3 townships (towns,street offices).Their urinary iodine level was determined by arsenic cerium catalytic spectrophotometry.Results Totally 1219 children aged 8 to 10 were examined,and their goiter rate was 4.92%(60/1219).Three hundred and sixty three urine samples were tested,and the median urinary iodine level was 223 μg/L.Among them,urinary iodine < 50 μg/L accounted for 5.2% (19/363),and < 100 μg/L accounted for 14.6% (53/363).The median urinary iodine level of pregnant women was 147.2 μg/L,and 52.0%(235/452) of them were less than 150 μg/L.The median urinary iodine level of lactating women was 134.1 μg/L.The consumption rate of qualified iodized salt was 94.4% (1143/1211).Per capita daily salt intake was 6 g,and 81.4% (293/360) of the residents' intake was less than 9 g.The median iodine content of drinking water was 6.2 μg/L,and 89.5% (68/76) of them was less than 10 μg/L.Conclusions All indicators have met the national standard of eliminating iodine deficiency disorders in Fujian Province in 2011.But there is an iodine deficiency problem in pregnant women,and these women should be given extra iodine supplement.
7.Expression of PURA gene and its protein in nephridial tissue of rats with fluorosis of coal-burning
Jia-qi, WANG ; Ji-can, LIU ; Yan-ni, YU ; Chang-wu, WAN
Chinese Journal of Endemiology 2010;29(2):135-138
Objective To investigate the meaning of PURA gene and its protein in nephridial tissue of the rats with endemic fluorosis of coal burning. Methods Thirty-six SD rats of 80 - 100 g, body weight were randomly divided into control group, low fluorosis group and high fluorosis group according to body weight, 12 in each group, the number of female and male in each group was the same respectively. The control group, Low fluorosis group and high fluorosis group rots were fed with 1.5,25.0,60.0 mg/kg fluoride content in feedstuff, to establish the animal model of fluorosis. Expressions of both mRNA and its protein of PURA gene in rat nephridium tissue, were determined by RT-PCR and immunohistochemistry after four-month experimental period. Results The expressions of PURA mRNA[(2.74± 1.06),(4.29 ± 2.11)] and its protein[ (28 827.91 ± 4801.94),(61 146.96 ± 4997.55)] in low fluorosis group and high fluorosis group was higher than that in the control group[ ( 1.13 ± 0.87), (7131.95 ± 1524.54), all P < 0.05]. And the expressions of PURA mRNA and protein in high fluorosis groups was higher than that in low fluorosis greup(all P < 0.05). Conclusion High fluoride can lead to the high expression of PURA gene mRNA and protein in the rat nephridium tissue exposed to sodium fluoride.
8.The awareness rate, treatment rate and control rate of mineral and bone disorder in patients with moderate or advanced stage chronic kidney disease
Jia-yi YAN ; Min-fang NG ZHA ; Zhao-hui Ni ; Rong JIANG ; Hai-fen ZHANG ; Yu-cheng YAN ; Wei-ming ZHANG ; Jia-ying HUANG ; Wei FANG ; Shan MOU ; Qin WANG ; Jia-qi QIAN
Chinese Journal of Nephrology 2012;28(1):10-15
Objective To investigate the awareness rate,treatment rate and control rate of mineral and bone disorder in patients with moderate or advanced stage chronic kidney disease (CKD). Methods The awareness rate,treatment rate and control rate of mineral and bone disorder were evaluated based on a questionnaire and related laboratory examinations in 503 CKD stage 3 to 5 patients. Results The awareness rate of mineral and bone disorder in patients with moderate or advanced stage CKD was highest in hemodialysis patients,moderate in peritoneal dialysis patients and lowest in non-dialyzed patients (all P <0.01).The total scores of the questionnaire were lowest in non-dialyzed patients [6 (5,8)] and were significantly higher in peritoneal dialysis [11 (9,12)] and hemodialysis patients [13 (11,15)] (P<0.01).The extent of awareness was negatively correlated with age (r=-0.11,P<0.05),and positively correlated with educational background (r=0.226,P<0.01),duration of CKD (r=0.597,P<0.01) and duration of dialysis (r=0.366,P<0.01).The source of knowledge was mainly from publicity and education made by medical staff,which accounted for 94.0%,79.5% and 69.4% respectively in nondialyzed,peritoneal dialysis and hemodialysis patients.The treatment rate was significantly higher in peritoneal dialysis (88.6%) and hemodialysis patients (96.9%) than that in non-dialyzed patients (58.2%) (all P<0.01).According to K/DOQI guideline,the control rate of serum calcium,phosphorus,calcium and phosphorus product and parathyroid hormone (PTH) were much better in non-dialyzed patients as compared to dialyzed ones.The percentage of number of lab indicators meeting the standard was significantly higher in non-dialyzed patients as compared to dialyzed ones (P<0.01).According to KDIGO guideline,the control rate of serum phosphorus was significantly lower in hemodialysis patients (23.6%) than that in peritoneal dialysis (36.9%) and non-dialyzed patients (46.7%) (P<0.01). Conclusions In non-dialyzed patients with moderate or advanced stage CKD,the awareness rate and treatment rate of mineral and bone disorder are relatively low,and the control rate is relatively high.Whereas in dialyzed patients,the awareness rate and treatment rate are relatively high,and the control rate is relatively low.
9.Intralesional bleomycin injection treatment for 44 cases of pharyngolaryngeal haemangioma.
Guo-jun LIU ; Qi-jun FAN ; Xue-jun LIU ; Li-yan NI ; Jin-jian GAO ; Sai-yu HUANG ; Bo-bei CHEN ; Jia-yun HUANG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2013;48(10):843-845
Adolescent
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Adult
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Bleomycin
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therapeutic use
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Child
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Child, Preschool
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Female
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Hemangioma
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drug therapy
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Humans
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Laryngeal Neoplasms
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drug therapy
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Male
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Middle Aged
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Pharyngeal Neoplasms
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drug therapy
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Young Adult
10.Effects of long-term oral administration of lanthanum nitrate on the liver of rats.
Ying LIU ; Dong CHEN ; Ai-jun CHEN ; Yu-xiu NIE ; Ran LU ; Jia-zuan NI
Chinese Journal of Preventive Medicine 2003;37(4):263-265
OBJECTIVETo probe the effects of long-term oral administration of lanthanum nitrate [La(NO(3))(3)] on morphological change in the liver, aftereffect of deposited La in the liver and their mechanism in rats.
METHODSYoung Wistar rats were divided into two groups, one fed with 0.1, 0.2, 2.0, 10.0 and 20.0 mg/kg of La(NO(3))(3) for six months and the other for the control. Changes in ratio of liver to body weight were observed after exposure to La(NO(3))(3) at varied doses for six months and one month after six-month exposure, as well as morphology of the liver in the rats with routine histochemistry and transmission electron microscopy (TEM) technique. Content of La in the liver was measured with inductively coupled plasma-mass spectrometry (ICP-MS).
RESULTSRatio of liver to body weight was significantly higher in the male rats exposed to 20.0 mg/kg of lanthanum for six months than that in the control group. Ratio of liver to body weight restored to normal in the rats exposed to 20.0 mg/kg of La one month after six-month exposure. Infiltration of inflammatory cells in the portal region of the liver, small amount of fat drops in hepatocytic cytoplasm, increased density of mitochondria stroma, lysosome containing highly-electronic-density bodies and dense granules, normal nucleus and slightly deformed nucleus of hepatocytes could be found in the rats exposed to 20.0 mg/kg. Areas of the liver deposited with glycogen after six-month exposure to 20.0 mg/kg of La accounted for (26.1 +/- 1.5)% and (4.1 +/- 1.4)%, respectively for male and female rats, significantly lower than those in the control group [(31.3 +/- 1.4)% and (39.4 +/- 0.9)%, respectively], with a statistical significance and very statistical significance, respectively. There was a little infiltration of inflammatory cells in the portal region of the liver one month after six-month exposure to 20.0 mg/kg of La, and amount of the dense bodies was lower in the rats exposed to La for six months. Liver contents of La in the rats of all experimental groups were lower one month after six-month exposure than those in the rats exposed for six months.
CONCLUSIONSExposure to a dose of 20.0 mg/kg La(NO(3))(3) for a long term could damage the liver structure to certain extent, but lanthanum deposited in the liver could be eliminated from the body gradually.
Administration, Oral ; Animals ; Female ; Lanthanum ; toxicity ; Liver ; drug effects ; metabolism ; pathology ; Male ; Organ Size ; Rats ; Rats, Wistar