?Retinoblastoma is a common eye malignant tumor which has high degree of malignancy.The traditional treatment methods are destructive, and the prognosis is poor. Hypoxia-inducible factor-1αhighly express in retinoblastoma and regulate the development and progression of retinoblastoma ( by influencing the proliferation and differentiation of tumor cells and involving in the generation of vascular mimicry ) . This article reviewed the role of hypoxia inducible factor-1αin the development of retinoblastoma which will initiate a new avenue for the treatment of retinoblastoma.

The sulfonylureas as insulin secretagogues,have formed a cornerstone of pharmacotherapy for type 2 diabetes over 50 years.Although sulfonylureas are effective antihyperglycemic agents,individual variability exists in drug response (i.e.,pharmacodynamics) and adverse effects which may be related to some genes.This article mainly reviews the advance of recent studies about drug metabolism and pharmacogenomics of sulfonylureas.

186 serum samples from 81 patients with gastric cancer were determined for circulatingimmune complexe (CIC) by using the polyethylene glycol turbidity assay. Compared with 105healthy blood donors, the mean value of CIC were significantly increased in pretreatment pati-ents (47.46?24.52vs.27.67?12.09), and the corresponding figures for stage Ⅰ+Ⅱ, Ⅲ and stage Ⅳpatients were 38.60?14.24, 42.04?13. 18 and 54.31?29. 15 respectively. The incidence of positivityof CIC in pretreatment patients was 32.1%. The mean value of CIC is significantlyhigher in patients whose disease could not be treated with radical surgery than in patientswhose disease could.CIC levels increased significantly during 11～45 days after radical operation.CIC levels were positive correlated with levels of serum IgA, IgM, C_3, and negative corre-lated with the rate of lymphocyte transformation. The patients found positive for ANA hadhigher incidence of positivity of CIC than those found negative for ANA.The clinical signifi-cance of CIC in patients with gastric cancer was also discussed.

AlM:To discuss the application of prism alternate cover test ( PACT) and perimeter strabismometry in strabismic operation.METHODS: A total of 145 patients with concomitant esotropia or exotropia, aging 2 ~ 50 years old, were included. They were examined by PACT and perimeter strabismometry before operation. The strabismic operations were performed, the orthotropia rate of postoperation was assessed.RESULTS:Using PACT and perimeter strabismometry, there was a significantly difference of deviating angle ( P<0. 05) in mild and severe strabismic group. But there was no significantly difference of deviating angle (P>0. 05) in moderate strabismic group. The functional cure rate of postoperation was respectively 70. 83%, 67. 01%; the clinical cure rate was 20. 84%, 24. 74%; invalid rate was 8. 33%, 8. 25% .CONCLUSlON: The application of PACT and perimeter strabismometry in strabismic operation design, referring to the eye axial length, this can be helpful of improving the cure rate postoperation and decreasing the danger of reopreation.

OBJECTIVE: To develop a rapid and sensitive fluorescent immunochromatographic strip for the detection of cefalexin residue. METHODS: The Eu-silica nanoparticles were synthesized by using RP-microemulsion technology. The Eu-silica nanoparticles conjugated with cefalexin monoclonal antibody was used as a fluorescent label. The fluorescent immunochromatographic strip for the detection of cefalexin based on competitive inhibition immunoassay format was developed. The cefalexin-ovalbumin and goat anti-mouse IgG were immobilized on the nitrocellulose membrane as the test line and control line. RESULTS: After experiment optimization, the developed strip could provide detection results within 30 min and the limit of detection of cefalexin was 25 ng·mL-1. There was no cross-reactivity with 12 kinds of antibiotics including cephalosporins and penicillin. CONCLUSION: The fluorescence immunochromatographic strip is rapid, sensitive, and easy to operate, which may be a powerful tool to detect antibiotics in the future.

Objective To assess the radiofrequency ablation (RFA) lesions in canine prostate with real-time harmonic gray-scale contrast-enhanced ultrasonography and investigate the feasibility to predict these lesions.Methods A total of 5 male beagle dogs took RFA of the prostate.Radiofrequency (RF) was delivered under transrectal ultrasound (TRUS) guidance.A lesion was created in the right and left lateral lobes of canine prostate,respectively.The examination of conventional gray-scale ultrasonography,power Doppler ultrasonography,real-time harmonic gray-scale contrast-enhanced ultrasonography were performed immediately after RF procedure.All animals were subsequently sacrificed and the prostates were harvested for pathologic analysis.Thermal lesion volume was calculated based on expected shape of ellipsoid using equation.The volumes of the calculated thermal lesion on contrast-enhanced ultrasonography in dogs were compared with the volumes obtained from the pathological specimens.Results The RFA lesions could not be imaged accurately with real-time conventional gray-scale or power Doppler ultrasonography.However,contrast-enhanced low MI perfusion imaging was able to have real-time monitoring for the lesion (no blood flow).The volume of lesions was measured with contrast-enhanced ultrasonography without significant difference with those of pathologic results.Ultrasonic imaging(conventional gray-scale or power Doppler sonography)of the ablation areas also had its own characteristics.Conclusion The real-time harmonic gray-scale contrast-enhanced ultrasonography appears to be an accurate modality for assessment of RFA lesions.Conventional gray-scale and power Doppler sonography had their own characteristics to evaluate the RFA lesion.

Objective:To study the effect of edaravone on EAE rats and the underlying mechanism.Methods:Wistar rats were immunized with GPSCH,and randomly divided into control group,EAE group,dexamethasone group,low dose of edaravone group and high dose of edaravone group.The morbidity of disease and clinical signs were observed.The pathological changes of spinal cord tissue sections were observed under light microscopy after HE staining and trichrome staining.The expression of Nrf2 and HO-1 was observed by immunohistochemistry.Results:Morbidity of high dose of edaravone group (8.33%) and DXM group (0%) was significantly lower than in EAE group (58.3%) (P

Digitalization of ancient medical documents has become an important platform for the development and protection of medical literature and digitalization of ancient medical documents based on ancient medical documents themselves will lead to a series of intellectual property rights. After the relationship between ancient medical docu-ments and protection of intellectual property rights was analyzed, the major intellectual property rights involved in current digitalization of ancient medical documents were pointed out with their solutions elaborated from the reasona-bleness, rationality, legality and normality.

BACKGROUND:Isokinetic test system has been widely used to evaluate the muscle function levels Whereas.the reports concerning effect of sport training on the development of muscle strength of hip joint in college students using isokinetic testing are few.OBJECTIVE:To assess the extensor and flexor muscle strength of hip joints in college students with isokinetic testing system METHODS:Totally 40 volunteers,including 20 male and 20 female students were selected.Kinitech isokinetic testing system,produced by Australian Kylingk Company,was used to test the flexion and extension muscle of hip ioints as the sequence of centripetal followed by centrifugal.The test speed was 60(°)/s as slow speed,180(°)/a as middle speed,240(°)/s as high speed.Peak torque(PT)and relative values of extension and flexion muscles,total power(TP)and relative total power were measured.RESULTS AND CONCLUSION:At the respective speed,PT and relative values of female students were greater than that of female students(P＜0.01).Atthe speed of 60,120 and 240(°)/s,flexor PT was smaller than that of extensor PT in male students,which was opposite in the female students,but the difference had no significance(P＞0 05).At the respective speed.TP of males were greater than that of female students(P＜0.01).In male students,TP and relative values of flexor were smaller than extensorat speed of 60(°)/s and 120(°)/s(P＜0.01),and had difference at speed of 240(°)/s(P＜0.05).Infemale students,TP and relative values had significant difference between flexor and extensor at speed of 60(°)/s(P＜0 01)and at speed of 120(°)/sand 240(°)/s(P＜0 05).At the respective speed,PT and relative values,TP and relative values in male students are all greater than females.These values decrease with the speed increasing.At the respective values,values of male students are bigger than females.

Objective To preliminarily evaluate the value of color power doppler ultrasonography in the diagnosis of sacroiliitis in ankylosing spondylitis(AS). Methods Fifty-seven sacroiliac joints in 31 patients with active AS and 40 sacroiliac joints in 20 volunteers were detected by color power doppler ultrasonography.The color flow signs inside the sacroiliac joints were observed,and the resistance index(RI) was measured. Results In active AS,color flow signs were seen in 55 joints,and the mean RI value was 0.53?0.08 in 45 joints,while the other 10 color flow signs represented reversed phase in diastolic phase on pulse doppler ultrasonography.In the volunteers,color flow signs were seen in 16 joints,and the mean RI value was 0.97?0.01 in 6 joints,while the other 10 color flow signs represented reversed phase in diastolic phase on pulse doppler ultrasonography. Conclusion The abnormal flow signs at the sacroiliac joints can be detected by color power doppler ultrasonography.Low RI values provide diagnosis evidence for active AS.