Background Wearing of soft contact lens,as one of the important methods to correct ametropia,has been widely applied at present.The clinically deleterious effects of soft contact lens have been reported,but researches about influence of apoptosis of corneal epithelial cells are scant.Objective This study was to detect and compare the ratio of apoptosis of corneal epithelial cells between the patients with contact lens wearing and without contact lens wearing,and explore the influence of soft contact lens wearing for long term on growth of corneal epithelial cells after injury.Methods A retrospective nest case-controlled study was designed.Forty eyes of 20 myopic patients with wearing of soft contact lens for ≥5 years and 40 eyes of 20 myopic patients without wearing of soft contact lens were included in Affiliated Hospital of Guiyang Medical College.All the patients received the off-flap epipolis laser in situ keratomileusis (Epi-LASIK) from 2010 October to 2011 June.The corneal flaps were collected during the surgery.The apoptosis of corneal epithelial cells was detected by flow cytometry(FCM),and the difference of apoptotic proportion between the two groups was compared using independent sample t test.Results The FCM scatter plot showed that compared to the without contact lens wearing group,the early stage of apoptotic cells with high staining annexin V and low staining proaium iodide (PI) were much more,and survival cells with low staining annexin V and PI were less,and necrotic and late stage of apoptotic cells with high staining of annexin V/PI were more in the with contact lens wearing group.The early stage of apoptosis proportion of corneal epithelial cells was (11.23 ± 5.31)％ in the with contact lens wearing group,and that in the without contact lens wearing group was (7.31± 5.43)％,showing a significant difference between them (t=2.754,P=0.008).Conclusions A long term wearing of contact lens induce more apoptosis of corneal epithelial cells,which can result in a disorder of structure and function of ocular surface during the wound healing stage.

Acupuncture Therapy ; Female ; Humans ; Polycystic Ovary Syndrome ; etiology ; therapy

Objective To observe the hindpaw withdrawal threshold (HWT), and the ultrastructure and expression of glia cell line-de-rived neurotrophic factor (GDNF) in sciatic nerve (SN) in chronic constriction injury (CCI) rats after pulsed radiofrequency (PRF). Meth-ods 30 Sprague-Dawley rats were divided into sham modeling-sham treating (SS) group, CCI-Sham treating (CS) group and CCI-PRF (CP) group. The right SNs of the rats in the CS and CP groups were ligated, and it was separated without ligation in the SS group. The CP group accepted PRF at the ligation 14 days after modeling, while the electrodes were placed without electricity in the SS and CS groups. Their HWT was measured before and 1, 7, 14 days after modeling, and 1, 7, 14 days after treatment. The right SN of ligation was observed under electron microscope 14 days after treatment, meanwhile, the GDNF expression was determined with enzyme-linked immunosorbent assay (ELISA). Results HWT was significantly shorter in the CS and CP groups than in the SS group after modeling, and it increased in the CP group 14 days after treatment compared with that of the CS group (P<0.01). The degeneration of SN significantly improved in the CP group compared with the CS group, while the expression of GDNF increased compared with that in the CS and SS groups (P<0.01). Conclusion PRF could relieve the CCI-induced neuropathic pain by upregulating the GDNF expression in the SN to prevent the SN from injury.

Tumor metastasis and recurrence have become a key to curative effect and long-term survival, and a hotspot of eurrent clinical oncology research. Recently, a survey of extracellular matrix protein 1 (ECM1) expression in different tumors indicated that ECM1, although not tumor specific, is significantly el-evated in many malignant epithelial tumors that gives rise to metastases, emphasizing its relevance in the cancer process. Herein, this article reviews the research progress of ECM1 in tumor.

Objective To confirmthe protective effect of nerve growth factor (NGF)on cadiac adrenergie nerve in rats after acute myocardial infarction(AMI)and its mechanism.Methods 120 Wister rats were divided in-to sham-operated group.AMI group and NGF group.The samples were taken after6 h, d,4 d-7 d and 14 d sepa-lately.Immunohistochemistry method WaS used to show the distribution of adrenergic nerve fibers.The density of them were calculated by Medical Pathobgical Image Analysis Processing System.Results ①The densities of adrenergic nerve fibers in AMI group were obviously lower than that of sham-operated group greatly in 6 h and 2 d.4 d-7 d and14 d later.②The densities of adrenergic innervation in NGF group were obviously higher than that of AMI group 7 dand 14 d later.Conclusion NGF has protection effect 011 cardLac adrenergic nerve after AMI in the rat.

Objective To detect the expression of extracellular matrix protein 1 ( ECM1 ) in primary liver cancer tissues, and explore its clinical significance in liver cancer metastasis. Methods Sixty cases of primary liver cancer tissues and adjacent tissues from 60 patients who were admitted to the Affiliated Provincial Hospital of Anhui Medical University from June 2008 to December 2009 were collected, and nine cases of normal liver tissues were collected from patients with liver trauma as control. The expression of ECM1 and the relationship between ECM1 and clinicopathological features of liver cancer were detected and analyzed using the immunohistochemistry and Western blot. All data were analyzed using the chi-square test, Fisher exact test and t test. Results ECM1 was mainly expressed in the cytoplasm of liver cells. The positive expression rate of ECM1 in liver cancer tissues was 73%, which was significantly higher than those in adjacent tissues (20%) and normal liver tissues (22%)( x2 = 34.286, 7. 044, P ＜ 0.05 ). The expression of ECM1 was correlated with liver cancer metastasis and TNM stages ( x2 = 5. 455, 4.275, P ＜ 0.05), while not with sex, age, size, capsule and differentiation of the tumor,alpha fetoprotein level and the expression of hepatitis B surface antigen ( x2 = 2. 841, 0. 014, 0. 000, 0. 734,0.075, 0.000, 0.031, P＞0.05). The result of Western blot indicated that the relative content of ECM1 in the liver cancer tissues was 25.49 ± 4.61, which was significantly higher than those in adjacent tissues (3.00 ±0.37) and normal liver tissues (2.94 ± 0.21 ) ( t = 31. 962, 31. 699, P ＜ 0. 05 ). Conclusions The expression of ECM1 in liver cancer tissues is higher than those in adjacent and normal liver tissues, and ECM1 expression is correlated with metastasis of liver cancer and TNM stages, which indicate that ECM1 may play a role in the metastasis of liver cancer, and it could be used as an indicator for liver cancer metastasis.

Objective By virtual screening in MDL,to search for a novel γ-secretase inhibitor.Methods A series of compounds were designed,synthesized,and evaluated based on pharmacophore model of γ-secretase inhibitors by virtual screening in MDL.Results The drug-likeness analytic data synthesized indicated that target compounds had drug-likeness.Each svnthesized compound was checked by IR spectroscopy,~1H and ~(13)C-NMR spectroscopy.Conclusion The designed compounds had better activity by model prediction.And the optimal compound showed a significant estimated activity value of 0.025 nmol/L and can be used as a lead for further drug development.

Objective To evaluate the feasibility and therapeutic effect of kyphoplasty in treating severe osteoporotic vertebral compressive fractures. Methods Thirty-five patients (48 vertebral bodies) with severe osteoporotic compressive fractures were included. There were 33 females and 2 males with the mean age of 74.2 years. The average compressive rate of the affected vertebral bodies was 77.0%. The thoracolumbar vertebrae were treated with kyphoplasties. Percutaneous puncture direction was adjusted according to compressive rate and shape of the vertebral bodies. The inflatable bone tamp was inserted into the fractured vertebral body. The balloon was inflated with low pressure and dilate-relieve-dilate method was applied. The balloon was deflated and withdrawn, leaving a cavity within the vertebral body, which then fulfilled with visualized bone cement. Preoperative and postoperative symptom level, complications and radiographic findings were recorded. Results All 35 patients tolerated procedure well. The mean heights of the anterior, mid and posterior vertebral body had improved from (0.8±0.1) cm, (0.8±0.2) cm, (2.1 ±0.8) cm preoperatively to (1.2±0.3) cm, (1.3±0.2) cm, (2.3±1.0) cm respectively after operation (P ＜0.05). There was significance difference between preoperative and postoperative heights of the anterior and mid vertebral body. The mean kyphosis was improved from 28.2°±5.2° before operation to 19.1°±4.9° after operation. Conclusion Kyphoplasty is feasible and effective for severe osteoporotic vertebral compressive fractures.

Objective To preliminarily evaluate the efficacy and safety of chemotherapy on hemophagocytic lymphohistiocytosis(HLH) with hepatic dysfunction in children.Methods The children diagnosed as non-malignancy-associated HLH from Mar.2004 to Apr.2008 were selected,and the therapeutic effect was evaluated according to the HLH-04 protocol at the 8th week of chemotherapy,and the level of serum alanine aminotransferase(ALT),serum albumin(Alb) and plasma fibrinogen(Fib) were detected at pretherapy,2 weeks and 8 weeks of post-treatment.Results Altogether 60 HLH children complicated with hepatic dysfunction before chemotherapy,47 children had increased ALT,58 children had decreased Alb,and 38 children had decreased Fib.Forty-two cases(70%) were virus-associated HLH,1 case(1.7%) was fungi-associated HLH,and 17 cases(28.3%) had unknown origin.Among the 60 children,55 cases showed improvement in the 4 weeks of inductive treatment,15 cases gave up therapy,45 cases completed the 8 weeks of inductive treatment according to the protocol(among these children,42 cases had no active disease,3 cases had active disease),and these 45 children had obviously improved ALT,Alb and Fib at 2 weeks and 8 weeks of post-treatment,compared with pretherapy,the differences had statistical significance(Pa

Day care is described in the paper in terms of its meaning and management.As to areas deserving attention given the initial success,the authors proposed to priortize medical insurance compensation policy,medical resource deployment,patient safety and follow-up for discharged patients.These efforts may further quality of care and patient satisfaction.