Acute appendicitis is the most common surgical or acute abdomen in childhood.Rapid and correct diagnosis is the premise of rational therapy,but there are still some problems in diagnosis and differential diagnosis.Sufficient comprehension about clinical features and differential diagnostic diseases,typical clinical symptoms,physical examination,laboratory examinations,ultrasonography or CT would make a definite diagnosis.Laparoscopy is also used to make a diagnosis if necessary.

Objective: To observe effects of Ganzhikang Capsule on ultrastructures in non-alcoholic fatty liver model rats so as to provide morphological basis for clinical treatment. Methods: Fatty liver model rats were induced by high fat diet, and the effects of Ganzhikang Capsule on microstructures in liver tissue were investigated with Dong Bao Gan Tai used as control medicine. Results: In the model group, serious fatty degeneration of hepatocytes with inflammatory infiltration, degeneration and necrosis, and deforming, swelling of mitochondrion, dilatation of endoplasmic reticulum were found. After treatment of Ganzhikang Capsule, microstructures of hepatocytes restored obviously, fatty degeneration relieved significantly, and Ganzhikang Capsule was superior to Dong Bao Gan Tai in restoration of fatty degeneration (P

Objective To investigate the curative effect of different biofeedback and electrical stimulation solutions on female stress urinary inconti-nence(SUI). Methods A total of 40 female patients with mild-to-moderate SUI were randomly divided into two groups(n=20 each group)and treated by the biofeedback and electrical stimulation. In the treatment group,the current increased in the middle of the treatment on the basis of the initial threshold of stimulus intensity to make a new tolerable threshold of stimulus intensity. In the control group,the patients were treated with the initial threshold of stimulus intensity during the whole treatment. The curative effects in these two groups were determined by the comprehensive strength of pelvic floor,the 1 h urine pad test results,and the life quality scores of urinary incontinence. Results After treatment,there were signifi-cant improvements in all the above indicators in two groups(P<0.05),and the results is better in the treatment group than in the control group. The effective rate in treatment group(95%,19/20)was higher than that in the control group(65%,13/20)(P<0.05). Conclusion The biofeedback and electrical stimulation were effective and safe in the treatment of women with SUI. The curative effect of patients treated with increasing current in the middle of the treatment is better,suggesting that it can be widely applied in clinic.

The etiology and pathogenesis of systemic lupus erythematosus(SLE) remain unclear. Interferon-γ (IFN-γ), a Th1-type cytokine, may be involved in the pathogenesis and inflammatory process of SLE. This article reviews reports on the serum IFN-γ of SLE patients and mouse SLE models, in vitro secreted IFN-γ, expression of IFN-γ induced protein and IFN-γ related genes, and treatment with traditional Chinese medicines.

Extremely severe pancytopenia induced by low dosage of azathioprine in systemic lupus erythematosus patients is rare. A 40-year-old Chinese female was diagnosed with systemic lupus erythematosus. She suffered worse erythema, oral ulceration, raised erythrocyte sedimentation rate and high anti-dsDNA in August 2013. Then she was initiated on oral azathioprine 50mg/d and extremely severe pancytopenia was seen in September 2013. She was recovered by a series of treatments. Regular monitoring of blood counts is highly recommended to reduce the possible serious myelosuppression induced by azathioprine.

Objective:To express secreted protein-pgp3 of Chlamydia trachomatis(Ct)plasmid,produce monoclonal antibodies (mAbs)and identify their basic biological characteristics.Methods: Construction pGEX-6p2-pgp3 prokaryotic expression vector,then expressed GST-pgp3 fusion protein in E.coli as antigen used to immune BALB/c mice, spleen cells were fused with SP2/0 mouse myeloma cells.The hybridoma cell lines of screening mAbs were secreted by ELISA,and mAbs specificity,type,class and titer were identified.Results:GST-pgp3 fusion protein was successful expressed,5 strains stable hybridoma cell lines that secreted mAbs were screened out,including 4 strains secreted anti-pgp3 mAbs(P1B3,P2A1,P2B6,P2C2),mAbs type were IgG1/κ,the other strain secretion anti-GST mAbs(P3B4),mAbs type was IgG2b/κ.The titer of P1B3,P2A1,P2B6,P2C2,P3B4 were 1∶6 400,1∶3 200,1∶12 800,1∶6 400 and 1∶6 400 respectively.Conclusion:Successful prepared anti-pgp3 mAbs,and lay a foundation for further study the function of Ct plasmid protein pgp3 and the establishment of Ct detection method.

OBJECTIVE:To establish an HPLC method for the determination of related substances in Donepezil hydrochloride tablets.METHODS:The separation was performed on AlltimaTM C18 column with mobile phase consisted of 0.01 mol?L-1 dipotassium hydrogen phosphate(3% triethylamine,pH=4.3)-methanol(60 :40) and flow rate of 1.0 mL?min-1.The detection wavelength was set at 271 nm and the column temperature was maintained at 35 ℃.The content of related substance was calculated by self-control with main component.RESULTS:Related substances were completely separated from the main constituent and the impurities were separated well from each other.The determination limit was 0.05 ng and the contents of related substances were 0.42%.CONCLUSION:The method is specific,sensitive and reproducible for the determination of related substances in Donepezil hydrochloride tablets.

Objective To investigate the heritability of Graves′ disease (GD) and to discuss its clinical significance. Methods The heritability of GD was calculated using Falconer′s formula and by investigating the GD numbers of 1968 first relatives of 526 established GD patients. Results The heritability of GD was 45%, appearantlymuchlowerthanthoseofprevious reports in China. Conclusion The onset of GD is affected by both inheritant and environmental factors, the latter seems to be predominant over the former.

[Objective]To evaluate the efficacy and short-term results of FASTIN anchor in cervical expansive open-door laminoplasty(ELAP).[Method]From February 2005 to February 2006,a total of 89 cases of cervical spondylotic myelopathy(CSM) were assessed in this study.Forty patients undergoing expansive open-door laminoplasty(ELAP) by FASTIN anchor were classified as study group.Fouty-nine patients undergoing conventional ELAP served as controls.The clinical results and radiological examinations of both groups were evaluated at 24 months after surgery.[Result]There were no significant differences in operation time,bleeding quantity and recovery rate of Japanese Orthopaedic Association(JOA) scores.The incidences of axial symptoms and C5 palsy in the study group were significantly lower than those in the control group(P

Objective To optimize the extracting conditions of Anchuan Keli. Method Six crude drugs, including Ephedra herb and so on, with the ephedrina hydrochloridum content as the index, extracting times, water volume and extracting time were screened by orthogonal test. Two crude drugs, including Sophora flavescens and so on, with matrine and ammothamnine content as the indexes, alcohol concentration, extracting times, alcohol volume and extracting time were screened by orthogonal test. Result The optimal extracting condition of six crude drugs including Ephedra herb was heat reflux and extracting 2 times (the first time 854 mL, the second time 732 mL), 2 hours for each time using water. The optimal extracting condition of two crude drugs include Sophora flavescens was heat reflux and extracting 2 times (the first time 288 mL, the second time 240 mL), 2 hours for each time using 80% alcohol. Conclusion The extracting technology selected is reasonable, practical and controllable.