Objective:To establish the experimental nude mouse model of endometriosis for human eutopic endometrium.Methods:Female nude mouse aged 6~8 weeks were selected.Nude mouse in experimental group(n=20)were implanted with the endometrium of patients with endometriosis or without endometriosis into pelvic and abdominal cavities.Nude mouse in control group(n=3)were implanted with the greater omentum.Nude mouse were killed at 5th,15th,and 30th day after implantation for observ ation of the growth of endometriotic lesions.The morphological and histological changes of endometriotic lesions from diferent sources and at diferent time points of growth were examined by light microscopy.Results:The achievement ratio of transplantation was 100%.At the 5th day,endometriotic lesions were found in the pelvic and abdominal cavities,and the adhesion to mice tissues was already tight.There were nascent vascular net between endometriotic lesions and mice mesepithelium.Endometrial glands around with stroma cells were found under light microscope.At the 15th day,endometriotic lesions mixed with mice tissues.Endometriotic lesions presented parenchymatous and cystiform.A lot of endometrial glands and stroma cells with rich blood supply were seen under light microscope.At the 30th day,endometriotic lesions became atrophied,and the adhension was serve partly.Endometriotic lesions presented severe fibrosis and some endometrial cells were not in integrity under light microscope.In the control group,some of the greater omentum adhered to the incisal opening of abdominal wall,while the others were free and atrophied or merged gradually.There was no change in the constructed greater omentum except for some inflammatory reaction.Conclusions:Nude mouse model of endometriosis was good for studying the endometria cell proliferation,neovascularization,hormone,immune response and so on,since the implanted endometrium remain the original morphological and histological features.It is a perfect model of endometriosis because of its significant and steady character,low cost,simple operation and short observation cycle.

Objective To research the effect of nimodipine in the prevention and treatment of delayed facial palsy after microvas-cular decompression .Methods A retrospective analysis was taken from January 2009 to March 2012 ,193 cases of patients with fa-cial spasm undergoing MVD in our department .According to whether applied vasodilators after operation ,all cases were divided into two groups :the experimental group(72 cases)with the treatment of nimodipine for two weeks ;the control group(121 cases) ,and not treated with nimodipine .The follow-up time was 6-12 months .Observe and compare the incidence ,level of delayed facial paraly-sis onset time and disease duration time between two groups .Results In the experimental group had 6 cases of late facial paralysis , the incidence was 8 .3% (6/72) ,onset time was (14 .5 ± 5 .2)days ,disease duration was (41 .3 ± 14 .4)days .The incidence of control group was 9 .1% (11/121) ,onset time was (12 .2 ± 7 .4)days ,disease duration was (55 .7 ± 36 .4)days .There was no significant difference between groups(P>0 .05) .According to House-Brackman classification ,there were 4 cases of grade Ⅱ and 2 cases of grade Ⅲ in experimental group .In control group ,there were 2 cases of grade Ⅱ ,6 cases of grade Ⅲ ,3 cases of grade Ⅳ .The differ-ence was statistically significant (P<0 .05) .Conclusion The delayed facial paralysis is influenced by a variety of causes .Vasodila-tor drugs can reduce the incidence of delayed facial paralysis ,and play a certain role in promoting facial paralysis restored .

AIM:To compare the effect of recombinant human epidermal growth factor eye drops and deproteinized calf blood extract eye drops on corneal edema after phacoemulsification.METHODS:Totally 72 cases (72 eyes) of patients undergoing phacoemulsification were selected and divided into the observation group and the control group by random number table method.After surgery,the observation group were treated with deproteinized calf blood extract eye drops while the control group were treated with recombinant human epidermal growth factor eye drops.The degree of corneal edema,subjective symptom score,corneal endothelium count,changes of corneal thickness and postoperative visual acuity recovery were compared between the two groups at different time points after surgery.RESULTS:Corneal edema in the two groups was significantly milder at 1wk after surgery than that on the 1st day after surgery (P<0.001).There was no significant difference in the degree of corneal edema and test results of visual acuity between the two groups at 1wk after surgery (P>0.05).Compared with 1d after surgery,the subjective symptom score and corneal thickness of the two groups significantly decreased on the 7th day after surgery (P<0.05).At 7d after surgery,the above indexes were significantly better in the observation group than the control group(P<0.05).At 1mo after surgery,the corneal endothelium count was significantly larger in the observation group than the control group (P<0.001).CONCLUSION:Both of recombinant human epidermal growth factor eye drops and deproteinized calf blood extract eye drops can significantly relieve corneal edema and improve visual acuity of patients after phacoemulsification.However,the latter has obvious advantages over the former in the repair of corneal endothelial cell injury after surgery.

This paper was to explore the application of case based learning method in medical psychology course for medical undergraduates.According to curriculum standard,nine cases were compiled which matched six articles,forming the complete instructional design.We followed the five steps of scientific teaching stage.The preliminary results showed that the case teaching was helpful to improving the medical students' knowledge acquisition,and their ability to master and solve practical problems.

Cricoid Cartilage ; surgery ; Humans ; Laryngectomy ; methods ; rehabilitation ; Larynx ; Recovery of Function

Carcinoma, Hepatocellular ; surgery ; Hepatectomy ; Humans ; Liver Neoplasms ; surgery

As a member of GTPase Rab family,Rab10 protein is not only involved in vesicle formation,transport,anchoring and fusion process,but also affects the occurrence and development of tumors.Research about the mechanisms of Rab10 in intracellular vesicle transport and tumor may provide a potential target and new idea for the anti-cancer therapy.

Objective:To establish a method for the separation and purification of haemocoagulase from agkistrodon blomhoffii in Changbai Mountain. Methods: An enzyme component with clotting activity was isolated from the venin of agkistrodon blomhoffii in Changbai Mountain by gel filtration, anion-exchange chromatography and heparin-Sepharose affinity chromatography. SDS-Page and RP-HPLC were used to determine its purity, SDS-Page was applied to study its action mode with bovine fibrinogen, HPSEC was used to determine the molecular weight, an IEF method was employed to detect its isoelectric point, and Lowry method was used for the deter-mination of protein concentration. Results:One haemocoagulase was purified from agkistrodon in Changbai Mountain. SDS-Page dis-played one band, and HPLC showed one single chromatographic peak. The haemocoagulase acted only on α chain of fibrinogen. Its molecular weight was 32. 2kD with isoelectric point of 5. 21. The enzyme had clotting activity in vitro. Conclusion:The method can be used for the separation and purification of haemocoagulase from agkistrodon blomhoffii in Changbai Mountain.

ABSTRACT:Objective To observe the influence of Osthole on cerebral ischemia-reperfusion injury and its acting mechanism.Methods We randomly divided 50 SD rats into sham (Sham)group,ischemia-reperfusion injury (IRI)group and Osthole (25,50,and 100 mg/kg)(Osthole)groups (n=10).The neurological symptoms, extent of cerebral infarction and cerebral water content were evaluated by physical approach.The activities of ROS and ATP were examined by the kit;the mitochondrial membrane potential (MMP ) was measured by flow cytometry.The expressions of Bax,Bcl-2,Caspase3,clevage-Caspase3,Caspase9,clevage-Caspase9,cytoplasmic AIF and cytochrome C were measured by Western blotting.Results Compared with those in sham group,the extent of cerebral infarction and cerebral water content,changes of neurological symptoms,activities of ROS,and expressions of clevage-Caspase3 ,clevage-Caspase9 ,Bax,cytoplasmic AIF and cytochrome C in IRI group were significantly increased (P<0.05).However,the activities of ATP,expressions of Caspase3,Caspase9,Bcl-2 and MMP,and ATP activity were decreased (P<0.05).Compared with those in IRI group,the extent of cerebral infarction and cerebral water content,changes of neurological symptoms,activities of ROS,and expressions of clevage-Caspase3 ,clevage-Caspase9 ,Bax,cytoplasmic AIF and cytochrome C in Osthole group were significantly decreased (P<0.05).But the activities of ATP,expressions of Caspase3,Caspase9,Bcl-2 and MMP,and ATP activities were increased (P < 0.05 ). Conclusion Osthole pretreatment can attenuate cerebral ischemia-reperfusion injury,which is associated with suppressing mitochondria-mediated apoptosis.

The authors searched the literatures about medical ethics published in CNKI from January 1 st,2005 to December 31st,2015 in the past eleven years.Totally 2171 effective literatures were included.Through the quantitative method of bibliometrics and qualitative analysis of content,the authors draw the main conclusions as following:there was a large number of publication of medical ethics,the direction of literature research was detailed,the universities should cooperate with hospitals,the quality of the literatures was satisfied,the research level was not much high,and so on.