Although the medicial sciences greately developed nowadays,diabetes mellitus(DM) which acts as a whole-life disease,remains a difficult problem to overcome.And it is a great challenge of lowering glucose levels effectively,preventing and postponing the onset of chronic complications to improve the quality of DM patients’ life.For better glycemic control,the writer who has many years of clinical experiences on DM therapy,combined results of several large-scale clinical trials,and then summarised the new trends of lowering glucose levels effectively,that is,"SELECT",which means sixkey points of glucose-lowering.

Objective To study the effects of NEP1-40 on axon regeneration, motor function recovery of affected limbs and RhoA signal pathway in cerebral ischemia-reperfusion rats for exploring the possible mechanisms. Methods A total of 60 adult male Sprague-Dawley (SD) rats were equally divided into sham operation group (sham group), cerebral ischemia-reperfusion control group (control group), intra-lateral ventricle injection of PBS group (PBS group), and intra-lateral ventricle injection of NEP1-40 group (NEP1-40 group). The middle cerebral artery ischemia-reperfusion model (MCAI/R) was established by nylon monofilament occlusion method in rats. The changes in growth associated protein-43 (GAP-43) and RhoA expressions were determined by Western blotting technique. The motor function of affected limbs was tested by the "staircase test" of Montoya’s design. Results ① The expression of GAP-43 in NEP1-40 group was higher than that in the control and the PBS groups at 7 d and 14 d after MCAI/R, peaking at 7 d; ② The expression of RhoA in NEP1-40 group was significantly lower than that in the control and the PBS groups at each time point; ③ The results of "staircase test" in NEP1-40 group were much higher than those in the control group and the PBS groups at each time point after MCAI/R. Conclusion NEP1-40 can improve axon regeneration in cerebral ischemia-reperfusion rats and promote the recovery of motor function. The mechanism may be associated with the inhibition of RhoA signal pathway.

Along with the advance in national economy, modern concept of burn rehabilitation from major burn injury implies that measures should be taken to help the patients return to society with dignity. This article briefly reviews the development and achievement of burn rehabilitation in our country, as well as the current difficulties in carrying out rehabilitation measures such as outmoded ideology, lack of trained personnel, low rat of popularization, outdated techniques and methodology, and relatively low level in scientific research, etc. The future development of burn rehabilitation in our country needs more social support, popular attention, and multidisciplinary joint efforts to help burn patients return to society with dignity. In order to fulfill this goal, we still have a long way to go.
Animals ; Burns ; rehabilitation ; China ; Humans ; Rats

Objective To investigate the role of rosiglitazone in high glucose(HG)-induced intercellular adhesion molecule-1(ICAM-1) expression of rat mesangial cells.Methods The rat mesangial cells(MCs) were cultured in the medium with normal glucose(5.6 mmol/L,NG),high glucose(25 mmol/L,HG),HG+5 ?mol/L rosiglitazone,HG+20 ?mol/L rosiglitazone and HG+PDTC(?NF-?B inhibitor).ICAM-1 mRNA expression was measured by semi-quantitative RT-PCR assay.Activation of nuclear factor-?B(NF-?B) of rat mesangial cells was measured by electrophoretic mobility shift assay(EMSA).The levels of ICAM-1 in the supernatants were determined by enzyme-linked immunosorbant assay(ELISA.) Results RT-PCR results showed that high-glucose increased the ratio of PCR products of ICAM-1 over GAPDH to 2.9-fold,which was prevented by rosiglitazone(5 and 20 ?mol/L) pre-treatment.The NF-?B binding activity was 2.5-fold higher in MCs exposed to HG as compared with NG(P

Objective To investigate the auditory brainstem response (ABR) of newborns in the neonatal intensive care unit (NICU) after hear screening failure.Methods To test ABR of 833 healthy newborns with no perinatal diseases at the clinic,and 425 newborns born in NICU who failed the hearing screening of monaural or both-ear from March 2014 to May 2015.Results 49.6% of children′s wave V response thresholds were ≥30 dB in the NICU group and 36.1% children′s wave V response thresholds were ≥30 dB in healthy group.The hearing abnormality rate of the NICU group was significantly higher than that of the healthy group(P<0.05);The average auditory thresholds healthy of the group[(19.76±6.59)dB] was significantly lower than those of the NICU group[(41.52±20.35)dB](P<0.05);both ear latency for waves Ⅰ,Ⅲ and Ⅴ and interpeak latency for waves Ⅰ-Ⅴ in healthy group were significantly shorter than those in NICU group (P<0.05).Conclusion The hearing loss extent of the NICU group is greater than that of the healthy group.The ABR abnormality reflects auditory pathway is abnormal.

Informed consent is the important thesis in life ethics and life law,which gives the patients and experimenters the right of self-decision making on medical procedures and experiments.As the development of public health and public health ethics,informed consent has been paid more attention,especially on informed consent problems in epidemiology and public health practice.The requirement of informed consent varies in different research fields.According to different practice purposes,the researchers choose relevant applicable methods.

OBJECTIVE:To observe the therapeutic effects of Lixiding(LXD)plus dobutamine(DBT)on severe acute congestive heart failure.METHODS:A total of40patients,including25patients with coronary heart disease,10hypertensive cardiopathy and5ectatic myocardiopathy,were enrolled in this study.Before and after combined use of LXD and DBT,the SBP,DBP,HR,CI,PCWP,TPR and LVEF were measured and the data obtained were detected with t test.RESULTS:After use of drugs,the SBP,DBP,HR,PCWP and TPR significantly decreased in comparison with those before medication(P

As targeted drugs to B-cell malignancies, anti-CD20 monoclonal antibodies have been proved to be important in therapeutic antibody field. With three generations in more than ten years' development, the structures of these drugs have been improved, and many new indications have been found. Nowadays, these kinds of antibodies are not only used in the treatment of lymphoid malignancies, but also been proved to be useful in some autoimmune diseases treatment, and their new indications are still being expanded. With the optimization of their clinical dosage regimens, drug reaction has been increased, thus, therapeutic and side effects of anti-CD20 monoclonal antibody have been further improved as well. However, the exact mechanism of action of their combination therapy with other chemical drugs is still unclear, which remains to be further studied. This article reviewed new development of anti-CD20 therapeutic monoclonal antibodies research in recent years.

Objective To observe the effects of salvianolic acid B on the stem cells factor(SCF) secretion and mRNA expression of mesenchymal stem cells(MSCs) in vitro.Methods MSCs were isolated,cultured and purified in vitro by adherent culture.The 3rd passage of MSCs was chosen to undergo the experiments.MSCs were cultured in five groups of lower glucose DMEM media containing different concentration of salvianolic acid B as follows: group of control(no salvianolic acid B added),four groups of salvianolic acid B treatment(0.03?g/ml,0.3?g/ml,3?g/ml and 30?g/ml of salvianolic acid B were added,respectively,into the lower glucose DMEM media).The quantities of SCF were measured by ELISA in the supernatant of cultured MSCs harvested at 24h,48h and 72h culturing.The expression of SCF mRNA in the supernatant of cultured MSCs harvested at 24h,48h and 72h culturing were analyzed with reverse transcription-polymerase chain reaction.Results RT-PCR assay displayed that dissolvent and membrane type of SCF mRNA expressed in MSCs basically.The expression of SCF mRNA and the quantities of SCF increased significantly(compared with the control group,P0.05).Conclusion Salvianolic acid B may promote the secretion of SCF and reinforce the expression of SCF mRNA of MSCs,implying that SCF might play an important role on the treatment of myocardial ischemia with the traditional Chinese medicine Salviae miltiorrhizae.

Aim To investigate the glycosidic constituents in the rhizomes of Alpinia officinarum Hance. Methods The isolation and purification of glycosides were done with column chromatography on macro porous resin, polyamides and Sephadex LH-20, whilst the structure elucidation was done by HRCI-MS and NMR (1D and 2D ) methods. Results A glycosidic ester identified as 4'-hydroxy-2'-methoxyphenol-β-D-{ 6-O-[ 4"-hydroxy-3", 5"-dimethoxy ( benzoate ) ] }-glucopyranoside ( I), along with a known compound n-butyl-β-D-fructopyranoside (II) , were isolated and characterized. Conclusion Ⅰ was found to be a new compound, named as alpinoside A, whilst Ⅱ was isolated from the genus Alpinia for the first time.