1.Stable expression of Hantavirus H8205 strain G1/IL-2 gene and immune protection of the fusion gene.
Ying, XIONG ; Yuan, YUAN ; Min, JIA ; Bing, YU ; Hanju, HUANG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2007;27(2):124-7
To explore the feasibility of stable expression of Hantavirus H8205 strain G1 segment and human IL-2 fusion gene in Vero cells, and to examine the immune protection effects on mice vaccinated with this recombinant eukaryotic expression vector containing Hantavirus G1 gene and IL-2 gene. With the help of lipofectamine, the Vero cells were transfected with pcDNA3.1/HisB-IL-2-G1 and the positive cells were selected by G418. IFAT and SDS-PAGE electrophoresis were used to determine the stable transfection and expression of recombinant protein. Each mouse was inoculated with plasmids intramuscularly (i.m.) three times, 2 boosts were given at 2-week intervals, serum anti-hantavirus antibodies were detected by ELISA and neutralizing antibodies (NAb) were detected by Plaque Reduction Neutralization Test. The fusion protein expressed in Vero cells was 78 kD, corresponding to the estimated molecular size. The neutralizing antibody titers of mice with pcDNA3.1/HisB-IL-2-G1 were 1:20-1:80. IL-2/G1 fusion gene could be transferred in Vero cells and stably express the fusion protein. Specific humeral immune responses in mice can be induced with the recombinant eukaryotic expression vector containing the fusion gene, which lays the foundation for further development of therapeutic HTNV vaccine.
2.Studies on saponins from seeds of Trigonella foenum-graecum Ⅲ. Isolation and structural elucidation for a new saponin D
Xuemin XU ; Jia WANG ; Hong YANG ; Weiping HUANG ; Chongjun YUAN
Chinese Traditional and Herbal Drugs 1994;0(06):-
Objective To investigate the saponins from the Chinese materia medica Huluba (the seeds of Trigonella foenum-graecum L.). Methods To isolate and purify the single saponin by the column chromatography and dry column chromatography of silica gel H. The structural elucidation was carried on by spectroscopic evidence of (()~(13)C-NMR), FAB-MS, DEPT and the results of the fraction-hydrolysis of acquiring the secondary glucosides. Results The newly isolated saponin D was consisted by six molecules of sugar with diosgenin. The chemical structure of saponin D is: diosgenin-3-O-?-L-rhamnopyranosyl (1→3)-?-D-glucopyranosyl (1→4)-?-L-rhamnopyranosyl [(1→3)-?-L-rhamnopyranosyl] (1→4)-?-D-glucopyranosyl (1→4)-?-D-glucopyranoside. Conclusion Saponin D is a new one consisted of six molecules of su-(gar) with diosgenin.
3.Studies on saponin from seeds of Trigonella foenum-graecum (Ⅰ) Isolation and structural elucidation for a new saponin A and its secondary glucosides
Xuemin XU ; Jia WANG ; Hong YANG ; Weiping HUANG ; Chongjun YUAN ;
Chinese Traditional and Herbal Drugs 1994;0(08):-
Object To investigate the saponin from the seeds of Trigonella foenum graecum Linn (STFG) Methods The total saponin from STFG was extracted and purified by using the absorptive resin, the single saponin was isolated by using the column chromatography as well as dry column chromatography of silica gel H The chemical structure was elucidated by 13 CNMR , FAB MS, DEPT spectroscopic evidence and the results of fraction hydrolysis of acquiring their secondary glucosides Results A new saponin A from the total saponin has been obtained, the fraction hydrolysis carried out and the secondary glucoside Ⅰ and Ⅱ identified by determining the structure of saponin A The chemical structure of saponin A is: diosgenin 3 O ? L rhamnopyranosyl(1→4) ? D glucopyranosyl(1→4) ? D glucopyranoside The secondary glucoside Ⅰ is: diosgenin 3 O ? D glucopyranoside; Ⅱ is: diosgenin 3 O ? D glucopyranosyl(1→4) ? D glucopyranoside Conclusion Glucoside A is a new saponin with three molecules of sugar
4.Studies on saponins from seeds of Trigonella foenum-graecum Ⅱ.Isolation and structural elucidation for two new saponins B and C
Xuemin XU ; Jia WANG ; Hong YANG ; Weiping HUANG ; Chongjun YUAN ;
Chinese Traditional and Herbal Drugs 1994;0(02):-
Object To do detail investigation of the saponins from the Chinese materia medica Huluba (the seeds of Trigonella foenum graecum L ) Methods The pure saponins from the total saponins were isolated by employing the column chromatography and dry column chromatography of silica gel H Their chemical structures were elucidated by 13 C NMR , FAB MS, DEPT spectroscopic evidence and the results of the fraction hydrolysis of acquiring their secondary glucosides were obtained Results Two new saponins B and C were isolated and both were the glucosides consisted by four molecules of sugar with diosgenin The chemical structure of B is: diosgenin 3 O ? L rhamnopyranosyl (1→3) ? L rhamnopyranosyl (1→4) ? D glucopyranosyl (1→4) ? D glucopyranoside And saponin C is: diosgenin 3 O ? D glucopyranosyl (1→4) ? L rhamnopyranosyl (1→4) ? D glucopyranosyl (1→4) ? D glucopyranoside Conclusion Saponins B and C are two new ones with four molecules of sugar respectively
5.Study on the acute toxicity of Tripterygium Hypoglaucum (Levl) Hutch solution in mice
Lujun YANG ; Houyong YU ; Ping HUANG ; Jian YUAN ; Jia CAO ;
Journal of Third Military Medical University 2003;0(17):-
Objective To explore the acute toxicity and LD 50 of Tripterygium Hypoglaucum (Levl) Hutch(THH) solution to provide information for safe clinical application. Methods After oral administration of THH solution in mice, the mortality and the physiological and pathological changes were observed. Results The LD 50 (95% confidence limit) of THH in male and female mice was 79 g/kg(69~89 g/kg) and 100 g/kg (90~112 g/kg), respectively. No marked pathological change of the organs was found. Conclusion According to the standard of grading of acute toxicity, THH solution belongs to the moderate class. Therefore, it is safe in clinical practice and has a wide application.
6.Two new triterpenes from Maytenus guangxiensis with their antiproliferative activity
Yuan-yuan HUANG ; Xue-gong JIA ; Fu-sheng DENG ; Jing-ya MO ; Jing-quan YUAN
Acta Pharmaceutica Sinica 2022;57(2):441-445
Four triterpenoids were isolated and purified from the 95% ethanol extract of
7.Evaluation on the accuracy of high-frequency ultrasound being used in the breast cancer screening program in women from Asian countries: a systematic review
Yuan HUANG ; Yi PANG ; Qiong WANG ; Jia-Yuan LI
Chinese Journal of Epidemiology 2010;31(11):1296-1299
Objective To evaluate the accuracy and value of high-frequency ultrasound for breast cancer screening in Asian women. Methods Published studies on high-frequency ultrasound for screening breast cancer in Asian women were systemically searched and assessed by the Quality Assessment of Diagnostic Accuracy Studies (QUADAS) tool. Meta-DiSc 1.4 software was used for extracting data, calculating the summary sensitivity and specificity, and drawing the Summary Receiver Operating Characteristic (SROC) curve. Furthermore, the proportion of screening of diagnosis on early breast cancer (TNM stage 0, Ⅰ and Ⅱ ) by high-frequency ultrasound was calculated. Results Seven screening studies including 22 244 women were selected for Metaanalysis. According to the QUADAS items, 5 studies were classified as A degree and 2 studies were evaluated as B degree. For study in the heterogeneous of these 7 studies (Q=38.97, P<0.0001 ),Random Effects Model (REM) was selected. The combined sensitivity (95%CI) and specificity (95%CI) were 0.785 (0.726-0.837) and 0.975 (0.973-0.977) respectively. The Area Under the Curve (AUC) of SROC was 0.9800. Among the follow-up studies of following period over one year, 96.9%of the diagnosed patients with breast cancer were at clinical stage Ⅱ or prior to it. Conclusion Because of its high accuracy, high- frequency ultrasound could be recommended for screening breast cancer in Asian women.
8.Intestinal absorption kinetics of Polygonum capitatum extract in rats.
Wu YANG ; Jia HOU ; Yuan LU ; Peng-cheng CHEN ; Shang-gao LIAO ; Yong HUANG
China Journal of Chinese Materia Medica 2015;40(21):4281-4287
A UPLC-ESI-MS/MS method was used to determinate the main active fractions gallic acid, protocatechuic acid, myricetrin, hyperoside and quercitrin in Polygonum capitatum extracts by in situ intestinal perfusion models; the absorption rate constants and cumulative penetration rate of absorption were calculated. The effect of different drug concentrations, different intestine segments, bile and P-gp inhibitors on the absorption mechanism of Gallic acid and other compositions in P. capitatum extracts. The experimental results showed that gallic acid, protocatechuic acid, myricetrin and quercitrin were observed saturated at high concentration (P < 0.05). Bile had significant inhibition effect on protocatechuic acid absorption and had promotion effect on myricetrin and hyperoside absorption (P < 0.05). P-gp inhibitor verapamil could significantly enhance the absorption of Protocatechuic acid (P < 0.05). The overall trend for absorption of various compositions was that small intestine > colon. This indicated that the absorption mechanism of P. capitatum extracts in rat intestine was in line with fist-order kinetics characteristics. The composition could be absorbed in all of the different intestinal segments, and the absorption was mainly concentrated in small intestine. The protocatechuic acid may be the substrate of P-gp.
Animals
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Drugs, Chinese Herbal
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chemistry
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metabolism
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Female
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Intestinal Absorption
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Intestines
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chemistry
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metabolism
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Male
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Polygonum
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metabolism
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Rats
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Rats, Sprague-Dawley
9.Effects of a compound Chinese medicine Xinji' erkang on isoproterenol-induced ventricular remodeling in mice.
Shan GAO ; Xinghui WANG ; Lingling HUANG ; Tingting YU ; Suming DU ; Yanwei GUO ; Yuan JIA ; Jian WANG
Journal of Integrative Medicine 2012;10(3):330-6
To investigate the effects of Xinji' erkang (XJEK), a compound Chinese herbal medicine, on isoproterenol-induced ventricular remodeling in mice.
10.Treatment of Herbert B2-type scaphoid fracture with double screws prograde internal fixation through volar approach.
Wei-Jie HU ; Jia-Yuan HONG ; Qiang LIU ; Li-Ping HUANG
China Journal of Orthopaedics and Traumatology 2014;27(3):203-206
OBJECTIVETo explore the clinical and function efficacy of the treatment of Herbert B2-type scaphoid fracture through volar approach with double screws prograde internal fixation.
METHODSFrom December 2005 to June 2010,18 patients with Herbert B2-type scaphoid fracture were treated with double screws prograde internal fixation through volar approach, including 14 males and 4 females with an average age of 33.11 years (ranged from 21 to 52 years). The fracture healing situation was evaluated by X-ray examination. Functional outcome was assessed by using Modified Mayo wrist score.
RESULTSAll patients were followed up with a mean time of (25.06 +/- 4.00) months. The bone healing time was (3.55 +/- 0.65) months. The pain,passive range of motion, grip of wrist were improved at 4 months after operation (P < 0.05). The Mayo wrist score were improved from preoperative 42.78 +/- 7.32 at 4 months after operation (93.89 +/- 5.83) (P < 0.05), the results were excellent in 9 cases and good in 9 cases.
CONCLUSIONVolar approach with double screws prograde internal fixation is an efficacy and safety surgical technique for Herbert B2-type scaphoid fracture.
Adult ; Bone Screws ; Female ; Fracture Fixation, Internal ; instrumentation ; methods ; Humans ; Male ; Middle Aged ; Palmar Plate ; surgery ; Range of Motion, Articular ; Scaphoid Bone ; injuries ; surgery ; Wrist Injuries ; physiopathology ; surgery ; Young Adult