Objective:To prepare loxoprofen sodium sustained release pellets, and investigate the in vitro drug release behavior. Methods:Loxoprofen sodium loaded pellets were prepared by extrusion-spheronization technology, and the sustained release pellets were prepared with Eudragit RL 30D and Eudragit RS 30D as the sustained release coating film materials. The drug release behavior of loxoprofen sodium sustained release pellets in vitro was studied. Results:Eudragit RL 30D and Eudragit RS 30D with the ratio of 20 ∶80 was used as the sustained release coating film materials, the coating weight was 20%, the plasticizer content was 10%, and the content of talc was 45%. The in vitro release of loxoprofen sodium from the sustained release pellets was steady and entire in 12 h. Conclusion:The release behavior of loxoprofen sodium sustained release pellets is quite satisfactory. And the preparation technology may be used in the industrial production.

This paper introduced the medical device classification in China and USA. Through the comparison between the two systems, several problems in Chinese classification system were exposed. To the end, some suggestions were proposed referred to the classification system of USA.
China ; Device Approval ; standards ; Equipment and Supplies ; classification ; United States

Evidence-Based Medicine ; Humans ; Resuscitation ; Shock ; therapy

Burns ; Evidence-Based Medicine ; Humans

Anterior Cruciate Ligament ; surgery ; Anterior Cruciate Ligament Injuries ; Anterior Cruciate Ligament Reconstruction ; methods ; Humans ; Knee Injuries ; surgery

Objective To investigate the effects of intravenous injection of α-crystallin on retinal ganglion cells (RGC) and some important organs of the Long Evans rats. Methods RGC were retrogradelabeled by fluorogold through bilateral superior colliculus and lateral geniculate body for seven days before optic nerve crush injury. Twenty-three Long Evans rats were used for this study, including three rats of normal control group and 20 rats of experimental group. Twenty rats were randomly divided into saline control group and three α-crystallin injection groups, which received tail vein injection of 1.25 ml isotonic saline and three different concentrations (1 × 10-2 , 1 × 10-1 and 1 g/L) of α-crystallin respectively, once every two days and totally seven times. After two weeks, the labeled RGC were counted, and the pathological changes on liver, kidney, brain, spleen and the lungs were investigated. Results Compared with the normal control group, although the number of RGC markedly decreased after two weeks of optic nerve crush injury in every group, the number of RGC in α-crystallin-treated groups was more than those in the saline control group. There were 2074± 150 RGC per mm2 in normal control group, 85 ± 15 RGC per mm2 in saline control group, 124±26 RGC per mm2 in 1 × 10-2 g/L α-crystallin group, 128± 31 RGC per mm2 in 1 × 10-1 g/L α-crystallin group, 164 ± 20 RGC per mm2 in 1 g/L α-crystallin group (F= 18. 660,P＜0. 01). No congestion, swelling, inflammation and other pathological changes were found in liver,kidney, brain, spleen and lung. Conclusions Intravenous injection of α-crystallin protein has protective effects on RGC after the optic nerve crush injury, and no significant effects on important organs.

The chitinase gene of baculovirus is an unnecessary and high conservative gene Chitinase is expressed in the late phase virus replication in insect cells There are signal peptide in its N termini, chitinases active motifs in its central section and ER attaching motifs of chitinase in its C termini Baculoviral chitinase possesses both endo and exo chitinase activities, which can hydrolyze inherent chitin in insects body Chitinase is necessary for liquefaction of the virus infected host insect, and it may also serve as a molecular chaperone for pro V Cath Perhaps Baculoviral chitinase has same ancestors with bacteriums chitinase

Hutchinson-Gilford progeria syndrome (HGPS) is a rare disease mainly characterized by accele-rated aging, with an incidence rate of 1 in 8 million to 1 in 4 million.It can affect the skin, fat, cardiovascular, bone and other organ systems.Most HGPS children can only live to 6-20 years old, with an average life expectancy of only 14.6 years.HGPS has distinctive clinical features, such as severe growth retardation, special skin manifestations, and craniofacial manifestations.The prognosis of this disease is poor, and no treatment has been proven effective so far.Upon the diagnosis, the progress of the disease should be observed and monitored via long-term and careful follow up, so as to extend the life span of the children as much as possible.In this article, the disease type, clinical manifestations, pathogenesis and clinical examination of HGPS were reviewed.

AIM: To establish a high performance liquid chromatography (HPLC) method to determine the concentration of valsartan in human plasma. METHODS: Using lrbesartan as internal standard, valsartan in plasma samples was determined by HPLC with liquid-liquid extraction, achieved by the column of Agilent ZORBAXSB-C_ 18( 150 mm? 4.6 mm, 5 ?m) at room temperature. The mobile phase consisted of a mixture acetonitrile : water : phosphoric acid : triethylamine was the ratio of 4060 1.0 1.5 (v/v), pumped at a flow rate of 1.0 ml?min~ -1, the wavelengths of fluorimetric excitation and emission were set at 265 and 378 nm respectively. RESULTS: The drug-free plasma did not interfere with the determination of drugs and internal standard. There was good linear relationships (1/C~2 weighted) between peak area ratio of valsartan to internal standard and C (r= 0.9996) within the range of 25-2 500 ng?ml~ -1. The precision of within-day and between-day was good. The lower limit of quantification was 25 ng?ml~ -1. The analytes reconstituted in the mobile phase were also stable at ambient conditions for at least 24 h. Furthermore, valsartan was stable for at least three freeze thaw cycles. CONCLUSION: The HPLC method can be used to determine the concentration of valsartan in human plasma.

Professor Dai Yuguang is good at treating many kinds of internal diseases.He thinks that the nasopharyngeal carcinoma is intimately connected with five viscera and two fu-large intestine and small instestine.Cold phlegm obstructs the lucid yang to get to nasopharynx.Radioactive rays burn the body fluid and interferes with zang-fu organs’ function.The poison of chemotherapy medicine is harmful to qi and blood,and blocks the transportation and transformation of the middle energizer.So,the principle to solve the problem is relieving the branch-aspect in acute cases;the methods include clearing away heat to remove toxin,replenishing qi and nourishing yin,etc.In the same time,to treat primary cause,dissipate phlegm and dredge orifices are needed.