ncreased. But it remains controversial how vaspin is correlated with insulin sensitivity. More studies based on large population are needed to identify the associ-ation between vaspin and insulin sensitivity.

Objective:To investigate the inhibitory effect and its possible molecular mechanisms of MicroRNA-34a(miR-34a) on the human nasopharyngeal carcinoma CNE-2 cell line subcutaneous xenograft tumor in nude mice.Methods: The human nasopharyngeal carcinoma CNE-2 cell line was cultured in vitro.miR-34a and Scrambled miRNA recombinant plasmids were successfully established and stably transfected into CNE-2 cells.Fifteen six-week-old male nude mice were divided randomly into three groups:miR-34a group(5 mice) ,Scrambled miRNA group(5 mice) ,Blank control group(5 mice).Different CNE-2 cells were subcuta-neously injected on the back near right lower limb.Tumor volumes were examined every 7 days.Mice were executed on the 35 days,and the eventual average tumor volumes and weights were examined.Total RNA and protein were isolated from tumors,and the expression of miR-34a,CDK6,and Bcl-2 mRNA and protein were determined by qRT-PCR and western blot,respectively.Results: The relative expressions of miR-34a was significantly up-regulated in miR-34a transfected group compared to Scrambled miRNA transfected group (P<0.05).Eight subcutaneous xenograft tumor models were successfully established.The nude mice of miR-34a transfected group appeared a smaller tumor volume compared to the other two groups at the beginning of 21th day( P<0.05).The eventual average tumor volumes in miR-34a group,Scrambled miRNA group and blank control group were(351.37±98.19)mm3,(798.75±91.04)mm3 and
(849.62±101.32) mm3 ,respectively,and the eventual average tumor weights in miR-34a group,Scrambled miRNA group and blank control group were(0.81±0.13)g,(1.47±0.21)g and(1.58±0.37)g,respectively.Both the eventual average tumor volumes and weights in miR-34a group were lower compared to the other two groups(P<0.05).qRT-PCR results revealed that the expression of miR-34a in miR-34a transfected group was significantly higher than in the other two groups,while the mRNA and protein expression of CDK6 and Bcl-2 were lower than the other two groups ( P<0.05 ) .Conclusion: miR-34a may inhibit the growth of human nasopharyngeal carcinoma CNE-2 cell line subcutaneous xenograft tumor in nude mice by down-regulating CDK6 and Bcl-2.

Objective To investigate the effect of implementation of QCC (quality control circle) in management of preoperative examination.Methods Through analysis of the common factors of patients' preoperative examination,the QCC was used to manage every parts of preoperative examination,and the nursing process of preoperative examination for resident patients was formulated.The effect of nursing management of preoperative examination before and after the implementation of QCC was investigated and compared.Results The result showed that the degree of satisfaction was significantly higher than before,and the risk incident was significantly lower than before.Conclusions To carry out QCC could improve the efficiency of preoperative examination and the patients' safety,as well as enhance the awareness of the nursing management.

The caudal-type homeobox gene is a member of homeobox gene—Para-HOX family,including CDX1,CDX2 and CDX4.As a transcriptional factor,CDX plays an important role in embryo development,hematopoietic system formation,intestinal epithelium tissue development and so forth.The abnormal expression of CDX is usually closely related with tumorigenesis.Researching the relationship between CDX and tumors will contribute to tumor diagnosis and treatment.

Objective To analyze the distribution of multidrug resistance bacteria infections in clinical departments and put for‐ward the corresponding countermeasures of prevention and control ,for providing basis for clinical diagnosis and treatment .Methods By carrying out the monitor of the drug resistance of bacteria ,the infection site and drug resistance strain and pathogenic bacteria distribution ,with 156 cases of multiple drug‐resistant bacteria infection ,were analyzed .Results The multidrug resistance bacteria of our hospital was distributed in the ICU unit ,accounted for 34% .Nosocomial infection of multidrug resistance bacteria was mainly the gram‐negative bacillus ,followed by the gram‐positive cocci ,and the majority of multidrug‐resistant bacteria were Escherichia co‐li(46 .2% ) ,Acinetobacter Baumannii(19 .9% ) ,Pseudomonas Aeruginosa(17 .9% ) ,Klebsiella Pneumoniae(17 .9% ) ,Staphylococcus Aureus(4 .5% ) .The priority was given to respiratory system infection ,accounted for 48 .1% ,followed by urinary system infection , accounted for 39 .0% .Conclusion The increase of multidrug resistance bacteria was very important problem that the hospital faced .It can effectively prevent and control multiple drug‐resistant bacteria infection by strengthening the monitoring of multi‐re‐sistant bacteria ,strictly executing disinfection and isolation measures ,the implementation of hand hygiene and reasonable application of antibiotics .

Objective To study the cause of blurred vision or cecitas after cardiac or cerebral angiography. Methods Six patients including 4 performed with cerebral angiography and 2 with cardiac angiography were analyzed. Results In those 6 patients, blurred vision happened in 4 cases, and cecitas appeared in 2 cases. Ophthalmologic examination revealed bilateral isocoria, thinning ophthalmic arteries and normal light reflex. Color Doppler flow imaging showed clearly the central retinal arteries. No cerebral infarction and brain hemorrhage were detected under CT. Conclusions Blurred vision and cecitas are the rare complications occurred with cardiac or cerebral angiography, probably with direct relationship to the concentration or dosage of the contrast media used, and the primary diseases of the patients.

OBJECTIVE:To optimize the extraction technology of Bushen huoxue capsules. METHODS:Central composite de-sign-response surface method was used to optimize the extraction technology with the amount of water and boiling time as main fac-tors using normalized value of the contents of icariin and salvianolic acid B,the yield of dry extract as index. Validation test was al-so conducted. RESULTS:The optimal extraction technology was as follows as 15-fold water,reflux extracting for 75 min,extract-ing for 2 times. The deviation of measured value and predicted value was 0.000 3% in validation test. CONCLUSIONS:The cen-tral composite design-response surface method can be used to optimize the extraction technology of Bushen huoxue capsules with good prediction,and the technology is simple and stable.

Objective: To study the difference in tooth profile among Chinese,Caucasian and Japanese with normal occlusion.Methods: The data of the measurments of Chinese,Caucasian and Japanese with normal occlusion were collected from published studies.Crown angulation,crown inclination and crown convex were used in the comparation among the peoples. Results: Tooth profile in Chinese was significantly different from that of Caucasian but was similar to that of Japanese. Conclusion: The orthodontic appliances should be modified when we use the straight wire appliances that is suitable for Caucasian and Japanese.

Objective To investigate the changes in non-linear dynamics properties of EEG and the application of real-time monitoring of non-linear indexes during the course of general anesthesia. Methods EEG was recorded in 65 patients undergoing operations. They were randomly divided into five groups: isoflurane, sevoflurane, desflurane (n=15, respectively), propofol 8mg/(kg?h) and propofol 10mg/(kg?h) (n=10, respectively). The EEG derived parameters correlation dimension (D 2 ) and approximate entropy (ApEn) non-linear indexes were calculated simultaneously during the whole operation, including the time of entering the operation room, anesthetic induction, intraoperation, recovery, and regaining consciousness. Results At the time of entering the operation room, their D 2 and ApEn nonlinear indexes appeared to be highest. Both the indexes decreased swiftly during anesthetic induction, and they dropped to lower values and leveled off during the intra-operation period. During recovery period, both of them rose gradually and returned to a high level in the post-operation awaking period. Conclusions Changes in the depth of anesthesia could be real-timely monitored and precisely measured with non-linear indexes of EEG. Non-linear dynamic analysis might provide us with more information on consciousness during general anesthesia

0 05) and neither implicit nor explicit memory in other groups. D 2 and ApEn nonlinear indexes were higher while entering the operative room. Both indexes dropped to a relative lower value and leveled off in the intra operative period. In 5mg group, both indexes increased significantly during the memory test compared with intraoperative period (D 2 and ApEn nonlinear index were 4 07 and 3 30, 0 67 and 0 53 respectively, P