ncreased. But it remains controversial how vaspin is correlated with insulin sensitivity. More studies based on large population are needed to identify the associ-ation between vaspin and insulin sensitivity.

Astrocyte elevated gene (AEG) -1 is cloned as a human immunodeficiency virus (HIV) -1-inducible and tumor necrosis factor-alpha (TNF-α)-inducible transcript in primary human fetal astrocytes (PHFA) by a rapid subtraction hybridization approach. AEG-1 has been reported to be up-regulated in various types of human cancers. Multivariate analyses indicat that AEG-1 correlates with the ability of growth, invasion, metastasis, angiogenesis and chemoresistance of tumors. AEG-1 over-expression activates the PI3K-Akt,nuclear factor kappa B (NF-κB) and Wnt-β-catenin signaling pathways in several crucial aspects of tumorprogression. AEG-1 represents a viable potential target for the therapy of human cancers.

Bidding invitation and procurement of medical equipment is one of the most important works in hospital.It can increase the working efficiency to hold the key taches of medical equipment procurement from 4 aspects including the science of decison-making,the comfirmability of bidding invitation,the right choice of the way of bidding,etc.So some important taches of medical equipment procurement are analyzed and discussed and some points for attention are raised.[Chinese Medical Equipment Journal,2008,29(2):76-77]

Objective: To investigate the expression and subcellular localization of G protein-coupled receptor 30 (GPR30) in Ishikawa endometrial adenocarcinoma cells. Methods: The expressions of GPR30 mRNA and protein in Ishikawa cells were detected by RT-PCR, Western-blotting and immunofluorescence assay. The positive expression rate of GPR30 in the cell membrane and cytoplasm was determined by flow cytometry (FCM). The subcellular localization of GPR30 labeled with colloidal gold was observed under a transmission electron microscope. Results: The expressions of GPR30 mRNA and protein were both detectable and predominately localized in the cell membrane and cytoplasm of Ishikawa cells. The positive expression rate of GPR30 protein in the cytoplasm [(20.10±0.13)%] was significantly higher than that in the cell membrane [(8.54±0.17)%] (P < 0.01). The subcellular localization of GPR30 protein was mainly in the mesh-like network of cytoplasm, which may refer to rough endoplasmic reticulum. Conclusion: The expression level of GPR30 is higher in cytoplasm than in cell membrane of Ishikawa cells. GPR30 is predominantely localized in the rough endoplasmic reticulum of Ishikawa cells, which suggests an important role of GPR30 in endometrial adenocarcinoma. Copyright© 2011 by TUMOR.

Dental Implantation ; Humans ; Surgery, Computer-Assisted

OBJECTIVE To investigate the clinical distribution and antibiotics resistance of Pseudomonas aeruginosa nosocomial infection after open-heart operation.METHODS The clinical data of 393 patients with nosocomial infection from Jan 2004 to Dec 2007 were analyzed retrospectively.RESULTS There were 57 infected cases caused by P.aeruginosa,and accounted for 14.5% of all infections during period.All infected patients had serious original heart diseases,and received broad-spectrum antibiotic therapy previously.Forty-two patients developed postoperative cardiorespiratory function failure,and 28 patients needed circulatory support.Thirty-six patients prolonged mechanical ventilation time for over 1 week.Results of susceptibility test showed that ciprofloxacin,levofloxacin and piperacillin/tazobactam were the most active antibiotics,followed by tobramycin,netilmicin,gentamicin,meropenem and imipenem/cilastatin.P.aeruginosa presented high resistance to ceftazidime and cefoperazone/sulbactam.CONCLUSIONS P.aeruginosa is one of the most common pathogenic bacteria after open-heart operations in our hospital and presented multidrug resistance.Rational use of antibiotics is important to reduce drug resistant strains.

Chromosome Aberrations ; Environmental Exposure ; adverse effects ; Humans ; Magnetic Fields

The embryo quality and endometrial receptivity is the two important factors that affects human implantation. Many patients with infertility caused by the disorder of endometrial receptivity fails in embryo implantation. Improving the receptivity of endometrium to improve clinical pregnancy rate has become a hot issue of research in recent years. In this paper, the current use of drugs and surgery on the measures to improve the receptivity of endometrium was summarized, in order to provide references for improving the success rate of assisted reproductive technology.

The present therapy for primary hepatocellular carcinoma (HCC)consists of surgery as well as local radiotherapy and chemother-apy.However,the majority of patients are susceptible to recurrence after comprehensive treatment,and the overall treatment outcome is not ideal due to the lack of effective drugs and strategies.Increasing evidence has demonstrated that the immune system is closely related to the development,progression,metastasis,and recurrence of HCC.Thus,immune therapy,especially cellular immunotherapy,could regulate immune function and induce specific antitumor immunity to achieve the goal of controlling HCC and reducing its recurrence and metastasis, which has become an essential part in the comprehensive treatment of HCC.The findings in preclinical and clinical studies on cellular immu-notherapy for HCC data are reviewed,and the current problems are discussed.

OBJECTIVE:To investigate the effects of triptolide(TP)on the proliferation of fibroblast-like synoviocytes(FLS) from patients with rheumatoid arthritis(RA)in vitro. METHODS:5 RA patients received knee arthroplasty or synovectomy to ob-tain synovial tissue. FLS was isolated,cultured and identified,and then incubated in the presence of TP [0 (blank control),50, 100 and 200 nmol/ml] for 24,48 and 72 h. The effects of TP on FLS was evaluated by MTT,and then proliferation inhibitory rate was calculated;flow cytometry was used to detect the apoptosis and cell cycle of FLS. RESULTS:The inhibitory rates of TP(50, 100 and 200 nmol/ml)on the proliferation of FLS were 17.46%-52.56%,which was positively correlated with drug concentration. Compared with blank control group,100 and 200 nmol/ml TP could increase the percentage of cells in G0/G1 phase and decrease the percentage of cells in S phase,with statistical significance(P<0.05);200 nmol/ml TP could induce cell apoptosis. CONCLU-SIONS:TP could inhibit the proliferation and also could induce the apoptosis of FLS in RA patients in vitro,which may be one of its mechanism for treating RA.