Objective To establish a method for determination of puerarin in Yangwei Soft Capsule. Methods HPLC was used for quantitative ananlysis. The Phenomenex Kromasil C18 (250 mm?4.6 mm,5 ?m) column was used,and mobile phase was composed of methanol-water (22∶78). Detection wavelength was at 294 nm,the flow rate was 1.0 mL/min,column temperature was at 40 ℃,the injection volume was 10 ?L. Results The linear response ranges from 0.058 8～0.882 0 ?g of magnolol (r=0.999 9,n=6). The average recovery of magnolol is 99.35%,RSD=1.94%. The linear response ranges from 0.020 4～0.306 0 ?g of honokio (r =0.999 8,n =6). The averagy recovery of honokio is 99.21%,RSD=2.10%. Conclusion The method is simple,accurate,and suitable for the determination of the purarin in compound preparations.

[Objective]To explore the combined curative effect of the ginkgo leaf and benazepril on the early diabetic nephropathy(DN).[Method]Ninety patients with the early diabetic nephropathy(DN)were randomly divided into single group(n=45,the ginkgo leaf injection 20ml,once a day for 3 months)and combined group(n=45,added benazepril 10mg,once a day for 3 months).The changes in glycosylated hemoglobin(Hba1c) and 24-hour urinary microalbum inuria were observed before and after treatment.[Result]Hba1c remained unchanged throughout the course of the treatment,urinary microalbum inuria decreased in both groups,a more profound reduction was achieved after the combined treatment(P

Aged ; Chondroma ; diagnosis ; Diagnostic Errors ; Humans ; Laryngeal Neoplasms ; diagnosis ; Male ; Tracheal Neoplasms ; diagnosis

Objective: To investigate whether plasma lipoprotein-associated phospholipase A2 (Lp-PLA2) activity correlates with severity and stability of coronary atherosclerosis and other established cardiovascular risk factors. Methods: Coronary angiography (CAG) was performed in 180 hospitalized patients who were suspected as having coronary heart disease (CHD). The severity of pathological changes of the coronary artery was assessed by the number of diseased coronary branches and Gensini's score. According to the results of CAG, the 180 patients were divided into 2 groups: CHD group (n=112) and non-CHD group (n=68). The CHD patients were further divided into subgroups according to the clinical types, the number of diseased coronary branches and Gensini's score. Lp-PLA2 activity, white blood cell (WBC) count, high sensitive C reactive protein (hsCRP), lipids, blood pressure and body mass index were measured. The age, sex and prior medical histories including hypertension, diabetes mellitus and smoking status were obtained before CAG in all patients. Lp-PLA2, WBC count and hsCRP were compared statistically between the subgroups, and correlation coefficients of Lp-PLA2 activity with WBC count, hsCRP and other conventional risk factors for CHD were calculated. Results: Plasma Lp-PLA2 activity in CHD patients was significantly higher than that in controls (P<0.01). No significant difference was found in Lp-PLA2 activity between patients with acute coronary syndrome and stable angina pectoris. Lp-PLA2 activity increased with the increasing number of diseased coronary branches and Gensini's score. Plasma Lp-PLA2 activity was positively correlated with TC and LDL-C, negatively correlated with HDL-C, weakly correlated with WBC count, and not correlated with hsCRP. Conclusion: Plasma Lp-PLA2 activity can be used as a parameter to predict pathological severity of coronary atherosclerosis, though it is not associated with stability of pathological changes of the coronary artery.

Objective To investigate the relationship between ?-catenin gene expression and the mechanism of tumorigenesis, development and metastasis of human nasopharyngeal carcinoma. Methods RT-PCR method was used to detect the mRNA expression of ?-catenin in normal human nasopharyngeal tissues and nasopharyngeal carcinoma. Results The results showed that ?-catenin expression in 11 out of 15 nasopharyngeal carcinoma specimens was decreased (73.3%). Conclusion The abnormal expression of ?-catenin changed in the normal adhesive function and the signal transduction pathway of the cell in human nasopharyngeal carcinoma, indicating that the expression level of ?-catenin was a biological marker of tumorigenesis and development of human nasopharyngeal carcinoma.

Objective To investigate the clinic characteristics,therapeutical approaches and outcome of food-borne infection caused by Group C,Type? hemolytic streptococcus.Methods We retrospectively analyzed 51 patients infected by Group C,Type? hemolytic streptococcus who were hospitalized in the First Hospital of Hebei Medical University from May to June 2003.Results All the patients had eaten the same food which was infected by Group C,Type ? hemolytic streptococcus.The clinical manifestations were 51 pharyngal hyperaemia(100%),44 fever(86%),39 tonsillitis(76.5%),31 headache(60.8%)and 23 ache from head to foot(45.1%).There was no obvious digestive symptom.Seventeen cultivated pharyngal swab samples had all positive results,and 23(52.3%)of 44 cultivated collutory samples were positive.The cultivated bacteria were all Group C,Type? hemolytic streptococcus equisimilies.All the patients were cured after antimicrobial therapy,and hadn't get any complication.Conclusion Infection caused by Group C,Type? hemolytic streptococcus should be considered if the patients have the same food and have such symptoms as fever,headache,ache from head to foot,pharyngal hyperaemia and tonsillitis.Diagnosis can be confirmed with the positive cultivated result of pharyngal swab and collutory.The patients without serious basic disease will have good prognosis after effective and timely therapy.

OBJECTIVE To strengthen the cleaning and disinfection management for endoscope to fulfil the specification.METHODS The management of cleaning and disinfection of the endoscopes were to be improved by methods of education,inspection,condition improvement and perfect institution.RESULTS The endoscopes which were disinfected were all reached the standards.CONCLUSIONS It is important to strengthen the cleaning and disinfection management for endoscopes.

Objective To evaluate the efficacy and safety of open occluded coronary artery branch through outside stent balloon expansion technique.Methods A retrospective analysis of 26 patients,26 interventional treat-ment of coronary bifurcation lesions were taken.All patients initially used conventional interventional techniques, branch reserve protection guidewire and main branch stent.When branch occlusion occured during operation and failed again through guidewire,small outer diameter of the balloon branch guidewire which was squeezed could be used to branch opening.The blocked branch opening was expanded outside of the stent,and the balloon was expanded after the guide wire enter into branch again.The main branch/branches kissing balloon inflation or main branch /branch double stenting would be used when necessary.Results Among 26 cases of bifurcation branch occlusion,25 cases successfully completed the outer stent balloon,achieved a branch balloon expansion after reentry guidewire,saved blocked branches and the rate of success was 96.2%,and 6 cases of the main branch and the branch kissing balloon dilatation,2 cases of remedial double stenting.Following -up for 1 -12(5.3 ±6.8)months after operation in 25 cases of patients,there was no death and myocardial infarction and other adverse cardiovascular events.Conclusion Out-side stent balloon expansion technique can improve the success rate of coronary occlusion branch opening,and it have fewer complications and worth of clinical application becasuse of its satisfactory results.

International Cooperation ; International Educational Exchange ; Medicine, Chinese Traditional

Objective To identify genes associated with hepatocellular carcinoma (HCC) as candidate diagnostic markers in a genome-wide scale. Methods The gene expression profiles of 40 pairs of HCC tumor tissue and peripheral non-tumorous liver tissue were analyzed by using gene chip technology.The gene chips were fabricated at the National Cancer Institute (NCI). Each gene chip contained 9 180 genes. The fluorescent targets were prepared by a direct labeling approach using two kinds of fluorescences as following: 100 ?g of total RNA from non-cancerous liver tissue was labeled with Cy3-dUTP and 200 ?g of total RNA from HCC was labeled with Cy5-dUTP. The targets were mixed together and hybridized with genes on the gene chips. Unsupervised hierarchical clustering analysis was done by CLUSTER and TREEVIEW software using median centered correlation and complete linkage. Results A total of 10 genes were found up-regulated in over 80% of primary tumors comparing with that of their corresponding non-tumorous liver tissues at a two-fold filter with an unsupervised hierarchical clustering algorithm, including protocadherin-alpha 9, ESTs, Homo sapiens cDNA FLJ, KPNA2, RPS20, SNRPE, CDKN2A, UBD, MDK and ANXA2. Conclusion These genes are supposed to be candidates for the diagnosis of HCC. Further investigation of these genes in a large scale of patients with HCC and patients with non-malignant hepatic diseases will be needed to disclose whether they could be used clinically as novel diagnostic tumor markers for HCC.