A zinc chloride and ethionine resistant mutant 0 5Eth400 5 was o btained from its parent strain Saccharomyces cerevisiae 346 by UV and 60 Co ? ray tr e atment and rational screening The glutathione productivity of the mutant reach e d 165 96mg/L by flask culture, which was 350% higher than the parent strain, an d the glutathione content in the dried cells reached 19 76mg/g, which was 318 6% higher than the parent strain A desecend of only 10 7% in the glutathione yie ld of the mutant was observed after ten times of subculture Therefore, the obtai ned mutant is stable strain that is worthwhile to be studied further

Objective To study the county high-risk women human papilloma virus (HPV)type distribution and subtypes of cervical intraepithelial neoplasia relevance,designed to provide a clinical risk assessment theoretical support.Methods 520 female patients with first visit high-risk HPV infection were selected.All subjects were HPV subtypes was measured and cervical intraepithelial neoplasia pathology,accounting analysis and correlation analysis and different subtypes of HPV accounting for patients with cervical lesions.Results 520 cases of HPV subtypes result patients showed that HPV infection subtypes,HPV16,HPV18,HPV31 and HPV58 infection higher proportion (44.8%,19.2%,11.5% and 16.7%),other types of infection was relatively low.520 cases of HPV infection in patients with CIN Ⅰ and CIN Ⅱ-Ⅲ and cervical squamous cell carcinoma was more,more than 15.0% of the total, a relatively small proportion of other types of cancer patients.In paragraph CIN Ⅱ-Ⅲ,HPV16 infection subtypes higher proportion of more than 60.0%,while the other was closer in cervical intraepithelial neoplasia,there was no difference between the written press and hold;in squamous cell carcinoma,accounting for HPV31 subtype only inferior HPV16(24.3%),which was significantly higher than the proportion of cervical adenocarcinoma (9.5%)(χ2 =3.821,P =0.049).Conclusion HPV infection in patients with high-risk subtypes higher risk of cervical lesions, require special attention,neoplasia different risks in different subtypes of patients infected cervical epithelium,clinical research is worth attention.

Objective To observe the effect of application of dexmedetomidine on cerebral injury in infants after cardiopulmonary bypass.Methods 60 cases of congenital heart disease were divided into 3 groups,each group 20 infants,namely D1,D2 and N group.Group D1 dexmedetomidine 1.0μg/kg,in 0.2μg · kg-1 · h-1 intravenous infusion,until the end of operation; group D2 with dexmedetomidine 0.5 μg/kg,in 0.1 μg · kg-1 · h-1,drug concentration and injection speed was the same as the group D1.Group N intravenous normal saline in the whole operation process,infusion speed was the same as the experimental group,anesthesia and experimental group agreement.Take blood samples sent to laboratory before the operation (T1),at the end of CPB (T2),after CPB 2 h (T3),6 h (T4),24 h (T5) respectively,colleced records of perioperative data.Results There were no significant in 3 groups,for infant's age,weight,operation time,CPB time difference (P ＞0.05).In T1,there was no statistical difference between the 3 groups in S-100β protein and NSE (P ＞ 0.05).In group N,S-100β protein and NSE in T2,T3,T4,T5 were increased significantly than the level in T1 (P ＜ 0.05).In T2,compared with group D2,S-100β and NSE increased significantly in group N and D1 (P ＜ 0.01),and there were more in D2 than D1 group.Differences in heart rate,blood pressure and hemodynamics were observed in infant during perioperative period was not significant (P ＞ 0.05).Conclusion Dexmedetomidine can reduce the ascensional range of S-100β and NSE in infant after cardiopulmonary bypass,and relieve the cerebral injury.

Arthritis, Juvenile ; complications ; Child ; Female ; Humans ; Macrophage Activation Syndrome ; complications

Objective To observe the renal expression of cyclooxygenase-2 (COX-2) in rats with type 2 diabetes, and explore the effect of selective COX-2 inhibitor Mobic on the expression of renal COX-2, matrix metalloproteinase-9(MMP-9), tissue inhibitor of matrix metalloproteinase-1(TIMP-1), TXB 2 and 6-Ket-PGF1?, as well as renal structure and function. Methods All rats were divided into control group, diabetes mellitus group and treatment group. Type 2 diabetic rats were treated with Mobic and vehicle respectively. Immunohistochemistry was used to detect the expression of COX-2,MMP-9 and TIMP-1 in renal tissues. The urinary TXB 2 and 6-Ket-PGF1? concentration was determined by radioimmunoassay at 6th week. Results There were an increasing expression of COX-2, TIMP-1 and decreasing MMP-9 expression in the renal tissue of type 2 diabetic rats. Mobic could increase MMP-9 expression and depress TIMP-1 expression througth inhibiting the expression of COX-2 in the renal tissues of type 2 diabetic rats. Conclusion COX-2 was involved in the pathogenesis of type 2 diabetic nephropathy. Selective COX-2 inhibitor Mobic might exert its renoprotective effects through inhibiting COX-2 activity, decreasing prostagladins systhesis, and modulating MMP-9 and TIMP-1 expression.

Objective To investigate the effect of postoperative analgesia with oxycodone on T cell function after operative of cesarean section with chronic hepatitis B.Methods Sixty cesarean sec-tion women with chronic hepatitis B undergoing CS,aged 22-35,were randomly divided into two groups:oxycodone group (group O)and morphine group (group M).The changes of immune cells (Th1,Th2)and liver function were recorded after the analgesia (immediate,postoperative 24 h,48 h,72 h).The total number of pressing analgesia pump and the cumulative amount of PCA were re-corded.Results The Th1 of group O was higher than that of group M at 24 h,48 h after operation (P <0.05),while there was no significant difference of Th1 and Th2 in group M.The total patient-controlled pressing times and accumulated amount of PCA of group O were significantly lower than those in group M (P <0.05).There was no significant difference in the incidence of adverse reactions between the two groups.Conclusion Oxycodone can activate T cell function in postoperative analge-sia,while morphine causes the inhibition of Th1 cells.

Simulated teaching method is a necessary complement to theoretical teaching of health law,which meets the practical-oriented reform need and cultivates students with the literacy as a legal professional.The teaching method demands more requirements on teachers,students,teaching materials,organizational management,and more attention on contextual design,preparation details,implementation activities and evaluation of teaching.

The construction of the model curricula is the important branch of teaching quality engineering. The key to the development of the model curricula lies in the reform of the teaching model. As required to the national standards of the model curricula and to the purpose of innovative educational philosophy,with the years of continuous exploration and practice,the clinical immunology examination curriculum has kept developing from the teaching staff,teaching condition,content of course,teaching method and means etc.,which has brought about satisfactory teaching effects. Thus it is forming the model curricula with special features.