Evidence-Based Medicine ; Humans ; Tinnitus ; diagnosis ; Vertigo ; diagnosis

Ear Diseases ; congenital ; diagnosis ; surgery ; Ear, External ; abnormalities ; Ear, Middle ; abnormalities ; Humans

Biomedical Research ; Humans ; Multicenter Studies as Topic ; Vestibular Diseases ; diagnosis ; therapy

Humans ; Vertigo

Audiology ; Deafness ; genetics ; Humans ; Otolaryngology ; trends

Anthropometry ; Female ; Humans ; Male ; Postural Balance ; Vision, Ocular ; Young Adult

Objective To optimize the water extractive process of Guben Zhike Electuary. Methods To arrange experiments with uniform design for optimizing suitable extractive proces, with the content of polysaccharide and astragaloside Ⅳ as the detecting indexes, inspecting the extacting times, lasting time and additional water which would affect the extraction rate of the two effective components. Result The best process was that water as extraction solvent, extracted for three times with two hours each time, and the amount of water added up to nine times of herbs. Conclusion The best process could extract most of the effective components out of the herbs, which is scientific.

Objective To discuss the probability of extracting the effective part of Yinhuang prescription with membrane separation method. Methods To extract and concentrate Yinhuang decoction with amb-class UF and first class NF, baicalin and chlorogenic acid were detected with HPLC, and polysaccharide with anthrone agent colorimetry. Results The content of polysaccharide in final membrane fraction was 3.41 mg/mL. The diversion ratio of baicalin and chlorogenic acid from primal decoction was 77.24%, 79.02%. Conclusion The effective part of Yinhuang decoction can be extracted with membrane separation method. Multiple active components including polysaccharide were retained and the method is simple.

Objective To investigate the effect of different subtypes of fractionated doses on multidrug resistance in ovarian cancer cells.Methods The human ovarian cancer cell lines SKOV3 and its drug-resistant subtype SKVCR were divided into four groups i.e., sham-irradiated, single dose (10 Gy),fractionated dose (2 Gy × 5 ) and multi-fractionated dose (1 Gy × 2 × 5).Cell sensitivity to vincristine(VCR),etoposide ( VP-16),pirarubicin (THP) and cisplatin (DDP) was measured by MTT assay.Western blot was applied to detect the expression of P-gp after irradiation.Results The doubling time of SKVCR was about 1.8-fold of that of SKOV3 cells.P-gp was expressed in SKVCR but not in SKOV3.IC50 values of SKVCR were higher than those of SKOV3.To SKOV3 cells,single dose irradiation decreased cell sensitivity to THP and DDP and fractionated irradiation decreased cell sensitivity to VCR,THP and VP-16.Multi-fractionated irradiation decreased cell sensitivity to VP-16.In SKVCR cells,all these irradiation treatments increased cell sensitivity to VCR and VP-16 but not to DDP.In addition,single and fractionated irradiation decreased P-gp expression in SKVCR cells.Conclusions Single,fractionated and multi-fractionated radiation induced chemotherapy resistance in SKOV3 cells,while reversed drug resistance to VCR and VP-16 in SKVCR cells.