Objective To study the effect of gastrin, motilin of chronic atrophic gastritis model in rats by acupuncturing at back-shu points. Methods 60 SD rats were randomly divided into blank control group, model control group, omeprazole group, back-shu points group, with 15 rats in each group, and to establish the model of chronic atrophic gastritis by irregular diet that given rats odd and even days. The blank control group, model control group were fed with normal saline, 2ml/time, 1 time/day. The omeprazole group was given oral gavage(Omeprazole 0.44 mg/kg, Clarithromycin 6.92 mg/kg, Metronidazole 7.6 mg/kg), 1 time/day. Back-shu points group received acupuncture at “Geshu”“Ganshu”“Pishu”“Weishu”“Shenshu”, retaining 15 min, 1 time/day. After 30 days, the content of ELSIA was used to detect the serum gastrin, motilin. Results ①GAS of model control group(54.25±5.70)pg/ml was significantly decreased compared with blank control group(66.63± 5.99)pg/ml, the difference was statistically significant (P<0.05). Compared with the model control group, GAS content of omeprazole group(62.70±8.55)pg/ml and back-shu Points group(66.27±3.79)pg/ml were higher, showed significant differences(P<0.05). ②MOT of model control group(223.60±17.83)pg/ml was significantly increased compared with the blank control group(188.45 ± 8.90)pg/ml, the difference was statistically significant(P<0.05). MOT of omeprazole group(200.16 ± 4.38)pg/ml and back-shu Points group(190.11±10.04)pg/ml were lower than those in the model control group, there was statistically significant difference(P<0.05). Conclusion Acupuncturing at back-shu acupoints can increase the content of GAS, reduce content of MOT, regulate gastrointestinal hormone in rats of chronic atrophic gastritis model.

Objective: To study the constituents from the sea cucumber Holothuria nobilis. Methods: The compounds of the sea cucumber were separated by multi-chromatography and their structures were elucidated by physico-chemical and spectral analyses. Results: Two new triterpene glycosides were isolated and their structures have been deduced by extensive spectral analysis (IR, 2D-NMR, and ESI-MS) and chemical structures as 3-O-{3″″-O-methyl-β-D- glucopyranosyl-(1→3)-β-D-glucopyranosyl-(1→4)-β-D- quinovopyranosyl-(1→2)-4′-O-sulfate-β-D-xylopyranosyl} -holosta-22, 25-epoxy-9-ene-3β, 12α, 17α, 25β-tetraol- sodium salt (nobiliside I, 1) and 3-O-{β-D-quinovopyranosyl-(1→2) 4′-O-sulfate-β-D-xylopyranosyl}-holoshillaside 18 (16)-lactone-22, 25-epoxy-9-ene-3β, 12α, 17α-triol sodium (nobiliside II, 2). Conclusion: Two aglycons are both new compounds named nobiliside I and nobiliside II.

Objective: To study the constituents from the sea cucumber, Stichopus variegatus. Methods: The crude saponins of S. variegatus were desulfated with pyridine-dioxane (1:1), the desulfated products were separated by multi-chromatography to afford two compounds. Results: On the basis of chemical methods and spectral evidences, especially 2D-NMR and ESI-MS, these two compounds were identified as 3-0-[β-D-xylopyranosyl-(1→2)-4′-O- sulfate-β-D-xylopyranosyl]-holosta-9-ene-3β,12α,17α, 25β-tetraol sodium salt, named as variegatuside A (1) and 16β-acetoxy-3-O-β-D-xylopyranosyl-holosta-22,25-epoxy-9-ene-3β, 12α,17α-triol, named as variegatuside B (2). Conclusion: Compounds 1 and 2 are new compounds.

Adult ; Hearing Loss, Noise-Induced ; etiology ; Hearing Tests ; Humans ; Male ; Middle Aged ; Noise, Occupational ; adverse effects ; Young Adult

Platelet-rich plasma(PRP) is a highly-concentrated plasma enriched in platelets and various growth factors. These growth factors will be released abundantly by platelets when PRP is activated by thrombin, which plays a key role in accelerating wound healing, bone repair, and meanwhile, in controlling of inflammatory process. Biocompatibility and versatility of PRP have been confirmed in clinical studies as well as its excellent effects on tissue reparation. These have lead to the extensive use of PRP in many medical fields as a therapeutic alternative. Mechanism of PRP in bone and tissue repair the application of PRP in the field of orthopedics is reviewed in this article. In addition, attempt is made to discuss about some problems still existing in the clinical application of PRP.

As a member of homeobox gene family,HOXA5 is expressed in many organs and can regulate gene expression,cell differentiation and morphogenesis.Its structure and (or) function abnormalities are closely related to the development of leukemia,breast cancer,brain hemangioma,hepatocellular carcinoma and so on.Researching the relationships between HOXA5 and tumors contributes to cancer diagnosis,treatment and prevention.

Objective To explore the impact of season and latitude on multiple sclerosis by study the onset/relapse season and latitude distribution in multiple sclerosis (MS) patients.Methods A total of 264 MS patients , with 88 males and 176 females, who were hospitalized in Beijing Tiantan Hospital from January 2002 to December 2012, were enrolled in the study and all the clinical data were retrospectively analyzed.The mean age was (33.9 ± 12.3) years old, with the disease duration of (6.3 ±4.5) years and 453 cases of relapse.The recurrence of MS was collected by four seasons, with March to May defined as spring, June to August as summer, September to November as autumn and December to February as winter.MS patients lived in Beijing (39.39° N-41.07° N) were chosen to test the correlation between the incidence/recurrence and monthly mean temperature, sunlight exposure intensity and duration.All the patients were divided into the high latitude group and the low latitude group, taken the latitude median (40.22° N) of Beijing area as the boundary.Gender composition, age of onset, disease duration and recurrence rate were compared between the two groups.Results Most of the onset/ relapse of MS were observed in winter (134 cases), while summer (97 cases) took the least.In the same latitude region (Beijing area), the onset/ relapse of MS was negatively correlated to the mean temperature and sunlight exposure intensity (r =-0.699, P =0.006;r =-0.623, P =0.015).Recurrence was higher in the high latitude group than in the low latitude group [68.7％ (123/179) vs 63.0％ (51/81), P =0.000], while no significant difference was found in gender composition, age of onset and disease duration between the two groups.Conclusion The onset/recurrence of MS has obvious seasonal characteristics.The onset/recurrence of MS is correlated with latitude, temperature and sunlight exposure intensity of the habitation of MS patients.Environmental factors are important cause of the onset/recurrence of MS, with sunshine exposure as the most key factor.

OBJECTIVE:To establish a RP-HPLC method to determine the content of ofloxacin in compound ofloxacin eye drops METHODS:Using Shim-pack ODS as fixed phase,a mixture to citric acid(0 05mol/L)-acetonitrile-ammonium acetate(0 5mol/L)-0 3% phosphoric acid(78∶22∶1∶3)as mobile phase,and detecting wavelength:293nm,flow rate:1 0ml/min RESULTS:The linear range of ofloxacin was 8 12?g～40 6?g(r=0 9 996,n=5) The average recovery was 99 29% and RSD(n=6)was 0 57% CONCLUSION:The method is rapid,simple and accurate

Bioglass is one of the most important inorganic biomedical materials and is widely used in clinic. Orthopedists and researchers pay more and more attentions to bioglass due to its excellent biocompatibility and bioactivity. This paper introduces the current research status and development of bioglass in bone repairing,bone tissue engineering, anti-infection drug delivery material and interventional therapy. Brief review is given to the potential application of bioglass in orthopaedics.

Objective To understand the status of the improper avademic behavior in medical journal,and to explore the prevention measures.Methods Total of 1 321 article in Zhongguo Yishi Jinxiu Zazhi were included in this study.Similarity detection system of Wanfang data and Google and baidu search engine were used to duplicate checking.The suspected existence of contributions for the improper avademic behavior,to check the whole paper of relevant original manuscript,and analyzed the characteristics of improper avademic behavior.Results One hundred and forty-five improper avademic behavior were found in 1 321 articles,accounted for 11.0％.The similarity of 58 paper were higer than 50.00％,accounted for 40.0％.Among them,the similarity of 10 papers were more than 90.00％,8 papers were 80.00％-89.99％,12 papers were 70.00％-79.99％,13 papers were 60.00％-69.99％,15 papers were 50.00％-59.99％,13 papers were 40.00％-49.99％,accounted for 9.0％,24 papers were 30.00％-39.99％,accounted for 16.6％,50 papers were 20.00％-29.99％,accounted for 34.5％.In 145 papers,copying accounted for 35.2％ (51/145),data forge or tamper accounted for 28.3％(41/145),breach of medical ethics and academic moral accounted for 28.3％ (41/145),fraud accounted for 6.9％ (10/145),a draft cast more accounted for 1.4％ (2/145).Original articles in the clinic were 92 papers,accounted for 63.4％;case reports were 28 papers,accounted for 19.3％;review were 25 papers,accounted for 17.2％.76 papers with a single author,accounted for 52.4％;38 papers with two authors,accounted for 26.2％,31 papers with more than three authors,accounted for 21.4％.Conclusions Contributions of suspected improper avademic behavior ratio is higher,and have certin characteristic.Medical journal editors should strengthen the responsibility consciousness,improve the ability to identify misconduct papers.