Based on computer and network technologies,the information system has grown into an important part for normal operation of hospitals,pushing up the importance of its risk analysis and defense.This article briefly described general methods and processes of risk analysis for information system,brought forward methods of risk classification and level-defense to identify and manage risks effectively,highlighted the importance of risk analysis,after-defense evaluating and tracking.This method is called into play to identify risks exposure of the information system of Beijing Red Cross Blood Center,contributing to a risk response table,developing a sustained improvement process for risk identification,control,appraisal and tracking,with defense carried out in specific system upgrading projects.

From Aug 2014 to July 2015, researches in the field of gonadal diseases have achieved rapid advances with image technology, the technology of biochemistry, and molecular biotechnology progressing. Considerable literatures concerning the pathogenesis, gene and clinical diagnosis, and treatment of gonadal diseases have been published. We reviewed and analyzed some more instructive literatures for clinical practice, proposed some suggestions, and the work plans for the clinicians.

Hepatitis C virus (HCV)infection is one of the most common causes of chronic hepatitis,liver cirrhosis,and hepatocellular car-cinoma.MicroRNAs (miRNAs),a class of small,non -coding RNA,are involved in a variety of physiological and pathological processes in human bodies.The mechanism by which miRNAs regulate HCV replication is described,and the effects of liver -specific microRNA -122 antagonists on hepatitis C antiviral therapy are discussed.Our study indicates that miRNAs play an important regulatory role in HCV ex-pression.Targeting miRNAs may be a potential therapeutic approach for treating HCV infection,but further studies are still in need.

Objective To evaluate the efficacy of treating female conyloma acuminatum with the CO2 laser under colposcopy guidance and traditional Chinese medicine sticking therapy.Methods Female patients with conyloma acuminatum whose diagnosis were confirmed by pathologic examination,were randomly assigned to two groups:a treatment group of 100 cases and a control group of 80 cases,with no statistical differences of the age and the duration.The treatment group was treated by CO2 laser to remove conyloma acuminatum and subclinical damage,and then by TCM wart-washing decoction sticking after operation; The control group was treated by CO2 laser to remove the wart,and then by TCM wart-washing Decotion for fumigating after operation.Results The curative rate of the treatment group and the control group was 79.00％and 60.00％ respectively.There was significant difference between the two groups (x2=7.723,P＜0.01).Conclusion The treatment of CO2 laser under colposcopy guidance combined with Chinese medicine sticking therapy is effective for female conyloma acuminatum.

Objective To evaluate effects of human papilloma virus(HPV)16E7 on expressions of six tumor suppressor genes(including MT1G, NMES1, RRAD, SFRP1, SPARC and TFPI2)in a cell line SiHa, as well as on its proliferation and apoptosis. Methods SiHa cells were divided into two groups to be transfected with a small interfering RNA targeting HPV16E7(E7SiRNA, experimental group)and an empty vehicle(negative control group) respectively, with SiHa cells receiving no treatment serving as the blank control group. After 48 hours, qPCR was performed to measure the mRNA expressions of E7 and six tumor suppressor genes, CCK?8 assay to evaluate cellular proliferative activity, and flow cytometry to assess apoptosis of SiHa cells. Results At 48 hours after the transfection, the experimental group showed significantly decreased E7 mRNA expression(0.25 ± 0.036, P<0.05), but increased mRNA expressions of the six genes(MT1G 1.403 ± 0.190, NMES1 1.720 ± 0.060, RRAD 1.390 ± 0.160, SFRP1 1.493 ± 0.120, SPARC 2.157 ± 0.144, TFPI2 2.060 ± 0.122, all P < 0.05). The proliferative activity of SiHa cells was significantly decreased(0.554 ± 0.130 vs. 1.028 ± 0.236 and 1.220 ± 0.126, both P<0.05), but the apoptosis rate was significantly increased(9.222%vs. 0.246%and 0.123%, both P<0.05)in the experimental group compared with the negative control group and blank control group. No significant differences were observed between the negative control group and blank control group in proliferative activity or apoptosis rate of SiHa cells(both P>0.05). Conclusion E7 may participate in HPV16?induced cellular malignant transformation by suppressing the mRNA expressions of 6 tumor suppressor genes, including MT1G, NMES1, RRAD, SFRP1, SPAR and TFPI2.

Objective To study clinical efficacy of ulinastatin combined with naloxone in patients with cardiogenic shock(CS) after acute myocardial infarction (AMI).Methods Eighty patients with CS after AMI were randomly divided into routine treatment group (n =19),ulinastatin group (n =20),naloxone group (n =21) and ulinastatin combined with naloxone group (n =20).The levels of serum cardiac troponin I (cTnI),brain natriuretic peptide(BNP),tumor necrosis factor-α(TNF-α) and interleukin-6 (IL-6)were measured before and a week after treatment.In the meantime,recovery time of shock,the average hospitalization days and 28-day mortality rate were recorded.Results After the treatment,the levels of serum cTnI,BNP,TNF-α and IL-6decreased in all groups(P ＜ 0.01),and there was significant difference on the decreasing degree of cTnI,BNP,TNF-α and IL-6 in ulinastatin combined with naloxone group when compared with those in routine treatment group,ulinastatin group and naloxone group(cTnI:(1.04 ± 0.17) ng/L vs.(2.06 ± 0.15) ng/L,(1.59 ± 0.16)ng/L,(1.97 ± 0.14) ng/L; BNP:(143.21-56.94) ng/L vs.(261.07 ± 71.43) ng/L,(203.46 ± 65.73) ng/L,(252.96 ± 68.85) ng/L; TNF-α:(13.42 ± 8.93) ng/L vs.(31.21 ± 12.32) ng/L,(20.39 ± 11.08) ng/L,(28.98 ± 11.76) ng/L ; IL-6:(37.58 ± 11.14) ng/L vs.(80.46 ± 27.15) ng/L,(59.84 ± 20.72) ng/L,(76.15 ±26.45) ng/L; P ＜ 0.01).The recovery time of shock,the average hospitalization days and 28-day mortality rate in ulinastatin combined with naloxone group were significantly lower than those in routine treatment group,ulinastatin group and naloxone group(recovery time of shock:(7.16 ± 1.52) d vs.(11.43 ± 2.40) d,(8.05 ±1.81)d,(8.74 ± 1.98)d;the average hospitalization days:(15.03 ±3.23)d vs.(22.64 ±4.18)d,(18.93 ±3.97)d,(19.21 ±3.94)d ;28-day mortality rate:(41.62％ vs.61.20％,50.74％,52.31％ ; P ＜0.01)).Conclusion The application of ulinastatin combined with naloxone can effectively inhibit the cardiac injury and inflammatory response,promote the recovery of circulation function and improve prognosis in patients with CS after AMI.

Objective: To confirm the optimum ingredient of the filler in Weiling Capsules (Rhizoma Coptidis, Fructus Evodiae, Fructus Toosendan and Radix et Rhizoma Rhei. Methods: The dissolution of berberine in weiling capsules of four different prescriptions were determined. Results: The T 50 , T d and m of the capsules of whole extraction, extraction which contains 1/4 raw drugs were significantly different from that containing 1/2 raw drugs ( P 0.05), and there is no significant difference between them ( P

Based on the basic data of China's total health expenditure from 2003 to 2015, this paper compares and analyze the structural characteristics of total health expenditure financing and the per capita disposable income before and after the new health care reform, in order to understand the overall level of total health expenditure financing before and after the new health care reform, whether the financing structural changes is reasonable and the overall trend is sustainability, and the relationship of the changes between per capita disposable income and health expenditure.The study found that, 1) although the growth rate of total health expenditure in China has declined after the new health care reform, the growth rate of personal health expenditure is still on the rise;2) the growth rate of government health insurance expenditure is limited to the substitution of personal health expenditure, and the growth rate of per capita personal health expenditure is still higher than the growth rate of urban and rural residents' per capita income;3) the problem of residents' serious burden of medical treatment is still very prominent.Based on the above problems, this paper accordingly proposes to speed up the reform of public hospitals, strengthen the construction of medical insurance fund specialization, and improve the ability of medical insurance fund to control costs, etc.

Objective To explore the relationship between HPV infection and the incidence of cervical canc-er and precancerous lesions in women.Methods 108 patients with cervical disease who accepted cervical screening were chosen as the research subjects, and they were given colposcopy and pathological examination, hybrid capture 2 generation method for quantitative detection of HPV-DNA content,RLU/CO was used for viral load and HPV-DNA content was more than 1ng/L(RLU/CO>1) can be judged as positive.Results The positive rates of HPV-DNA was 35.00%,67.74%and 95.83%of chronic cervicitis,cervical cancer precursor lesions( CIN) and invasive cervical squamous cell carcinoma,the difference was statistically significant(χ2 =10.73,P<0.05) .The positive rates of HPV DNA in patients with typeⅠCIN,typeⅡCIN,typeⅢCIN were 44.44%,62.50%and 95.00%respective-ly,the difference was statistically significant(χ2 =11.43,P<0.05).HPV-DNA and the positive rate of cervical cancer patients with histological grade,clinical stage,lymph node metastasis,there was no significant correlation(χ2 =1.76,1.44,1.89, all P >0.05 ) .Conclusion HPV -DNA positive rate increased with the severity of cervical lesions in patients,but it had no significant correlation with clinical and pathological parameters,the active prevention of HPV infection of cervical cancer prevention had important significance.

Objective:Defensins from human neutrophils exhibit a broad antimicrobial activity.The transfection of the gene fused by human neutrophil peptide 1(HNP1) with the J chain into other cells may induce the in vitro expression of HNP1 to play antimicrobial roles.The authors analyzed the influencing factors on the expressed proteins of HNP1 and the J chain gene in COS-7 cells.Methods: Western blot was used to analyze the technical factors influencing the HNP1 protein expression.Results: Recombinant J-HNP1 was expressed in the cells successfully.Conclusion: The expression of J-HNP1 can be detected inside and outside the cells by Western blot.