BACKGROUND:Pancreas or islet cel transplantation and stem cel transplantation bring hope to cure diabetes, but pancreas or islet transplantation appears to have a lack of donors as wel as immune rejection problems, limiting their clinical development. Therefore, stem cel transplantation therapy has become the current hotspot.
OBJECTIVE:To study the therapeutic effects of huaman telomerase reverse transcriptase (hTERT)-modified bone marrow mesenchymal stem cel s transplantation on diabetes mel itus in SD rats.
METHODS:Bone marrow mesenchymal stem cel s were transfected with PLXSN carrying hTERT. Thirty-six male SD rats were randomly divided into control group (n=6), stem cel group (n=10), hTERT transfection group (n=10), diabetes mel itus group (n=10). Except the control group, the rats were injected with stretozotocin (45 mg/kg) to make diabetes mel itus models. After modeling, rats in the stem cel group and hTERT transfection group were respectively intravenously injected with 1 mL of bone marrow mesenchymal stem cel s (1.5×1010/L) and 1 mL of hTERT-modified bone marrow mesenchymal stem cel s (1.5×1010/L).
RESULTS AND CONCLUSION:At 24 hours after modeling, the fasting blood-glucose level was significantly increased in the diabetes mel itus group, which was higher than the normal value (6.7 mmol/L). At 15 days after cel transplantation, the fasting blood-glucose levels were signficiantly decreased in the stem cel group and hTERT transfection group as compared with the diabetes mel itus group (P<0.05), but the body mass of rats was increased in these two group (P<0.05), especial y in the hTERT transfection group. At 45 days after cel transplantation, the fasting blood-glucose level and body mass in the stem cel group and hTERT transfection group were close to those in the control group (P>0.05), and moreover, the hTERT group had better outcomes than the stem cel group. Meanwhile, in the diabetes mel itus group, the fasting blood-glucose level was stil at a higher level, and the body mass decreased continously. These findings suggest that hTERT-modified bone marrow mesenchymal stem cel transplantation is effective for treatment of diabetes mel itus in rats.

BACKGROUND:Studies have reported that underin vitro experimental environment, antisense endothelin nucleic acid nanometer carrier can express target nucleic acid and produce RNA interference effect after enfolding by 12-alkylated chitosan nanoparticles, which can effectively inhibit the excessive generation of endothelin from inflammatory cytokines induced by alergen. OBJECTIVE:To investigate the effect of 12-alkylated chitosan nanoparticles enfolding antisense endothelin converting enzyme RNA expression plasmid on airway hyperresponsiveness in asthmatic mouse models. METHODS: Forty Balb/c mice were randomly divided into four groups: control, chitosan nanoparticles, normal saline and plasmid groups. Mice in the chitosan nanoparticles, normal saline and plasmid groups were subjected to sensitization by an intraperitoneal injection with ovalbumin (0, 14 days) and motivation by aerosol inhalation of ovalbumin (24, 25, 26 days) to induce asthma models. Mice in the control group were subjected to sensitization and motivation by the perfusion of normal saline. At 24hours before the first excitation, mice in the control, chitosan nanoparticles, normal saline and plasmid groups were perfused with normal salinevia airway, 12-alkylated chitosan nanoparticles, normal saline and antisense endothelin converting enzyme RNA. At 48 hours after the last excitation, the airway reactivity of mice was detected. After 28 days, bronchoalveolar lavage fluid cytology, lung histopathology, cytokines in spleen cel culture supernatant were detected. RESULTS AND CONCLUSION:Compared with the control group, the total number of plasmid cels, the percentage of eosinophils, eosinophil absolute counts, interleukin-4 levels, endothelin levels and airway hyperresponsiveness of mice in chitosan nanoparticles, normal saline and plasmid groups were increased (P < 0.05), and the lung inflammation was more severe. These indicators in the chitosan nanoparticles group were al lower than those in the normal saline and plasmid groups (P < 0.05) and the degree of inflammation was lighter than that in the saline and plasmid groups. These results demonstrate that 12-alkylated chitosan nanoparticles enfolding antisense endothelin converting enzyme RNA expression plasmid can reduce the synthetic amount of asthma endothelin and inhibit airway responsiveness.

Primary liver cancer ( PLC) is one of the most invasive malignant tumors in the clinics .The PLC within Milan criteria can be cured by liver transplantation or liver resection .However,PLC exceeding Milan criteria has been a question for us to overcome all the time .In recent years ,transcatheter arterial chemoemboliza-tion( TACE) and its combination therapy for PLC exceeding Milan criteria have made some breakthrough .A great deal of literatures have confirmed that TACE and its combined therapy for PLC exceeding Milan criteria can im -prove the survival rate obviously,it also contains many advantages,such as low cost,safe,painless,minimally in-vasive,repetitive operation and so on .The progress of TACE for PLC exceeding Milan criteria in recent years is summarized in this paper .

Objective To compare cerebral oximetry(rSO 2 %)and somatosensory evoked potential (N 35 ) in their accuracy in monitoring cerebral perfusion during carotid endarterectomy procedure under general anesthesia. Methods Ten patients (6 males, 4 females), scheduled for selective carotid endarterectomy, were enrolled in the study. Their rSO 2 %and N 35 were also continuously monitored and recorded during perioperative periods. The patients' cognitive function was evaluated at the same time. The MAP, HR, SpO 2 were continuously monitored during operation. Results There was a significant difference in rSO 2 % between that at the period of elamping and that at the period of declamping of the carotid artery. No patients suffered from cognition dysfunction during perioperative period. Conclusion With comparison to the amplitude of N 35 it was rSO 2 % which was earlier to show alteration in cerebral perfusion in case of cerebral oxygen deficit. In monitoring cerebral perfusion, rSO 2 % was better than N 35

Objective To explore the clinical value of tuberculous infection of T cells spot test (T-SPOT. TB)for diagnosing tuberculous pleurisy in immunocompromised patients. Methods The sensitivities of T-SPOT. TB,ADA, TB-DNA and tuberculosis antibodies to tuberculous pleurisy in 54 immunocompromised patients were compared.Among these patients,22 were retested by T-SPOT. TB after 2 months anti-tuberculosis treatment. Spot forming cells(SFCs) were compared before and after treatment. Results The diagnostic sensitivity of T-SPOT.TB (79.63%)was significantly higher than those of ADA (64.81%),TB-DNA (48.15%)and tuberculosis antibodies (55.56%) (P<0.05).22 patients were retested by the T-SPOT.TB after anti-TB treatment (2 months), the results showed that the positive rate of T-SPOT.TB was 90.91%,not significantly than previous tests (P<0.05).Both ESAT-6 and CFP-10 specific SFCs decreased significantly compared with those before anti-TB treatment (t=2.728 and 2.400,P<0.05 respectively). Conclusions T-SPOT.TB assay has satisfactory sensitivity and could be used in diagnosis of tuberculous pleurisy in immunocompromised patients.

Biology-related disciplines in medical school of comprehensive university are cur-rently confronted with some difficulties, including unclear position and indistinguishable characteris-tics. In this paper, we proposed that biology-related disciplines in medical school of comprehensive university should center on disease research, emphasize medical feature and give full play to its tech-nical advantage thus to provide technical support for basic medicine and clinical medicine. Mean-while, sources of multiple disciplines should be effectively integrated by breaking through the limitation of discipline and administrative system. An interdisciplinary molecular medicine platform was built up for researching and teaching.

In this paper, POCT detection technology which has been carried out in community health institutions is summarized and introduced. The paper has made the research on the existing resistance and urgent problem of POCT in the community. At the same time, the paper described the prospect of the development of POCT detection technology in community health institutions in view of the future.
Humans ; Point-of-Care Systems ; Public Health

Background Glaucoma is a kind of psychosomatic disorder.Investigating the psychological and behavioral characteristics of glaucoma patients has important implication for the prevention and treatment of the disease.Objective Present study aimed to characterize the personality profile of initial diagnosed glaucomatous patients.Methods Eighty-seven patients with primary open angle glaucoma (POAG),ninety-two patients with primary angle-closure glaucoma (PACG) and eighty-nine normal controls (normal control group) were enrolled in this study from January 2008 to May 2014 in Peking University People 's Hospital.Clinical and demographic information which could relate to personality type was collected by filling in Symptom Checklist 90 (SCL-90),Life Event Scale (LES),Eysenck Personality Questionnaire (EPQ),Self-Rating Anxiety Scale (SAS) and Self-Rating Depression Scale (SDS) voluntarily under the informed consent.The psychological,personality and behavior characteristics of the individuals were analyzed and compared among the different populations.Results In SCL-90,the scores of compulsivity,interpersonal sensitivity,depression,anxiety,hostility,phobic anxiety,paranoid ideation and psychoticism inpatients with primary glaucoma were significant different among the three groups (all at P＜0.01),and the scores were significantly higher in the patients of the POAG group and PACG group than those in the normal control group,and the scores were increased in the PACG group compared with the POAG group (all at P＜0.05).In LES,the scores of family events,social intercourse and other events,life events,stress response were significantly different among the three groups (P =0.032,0.002,0.001,0.000),and these scores were significant higher in the POAG group and PACG group compared with the normal control group,and the PACG group also had a significantly greater frequency of clinically abnormal score than the POAG group (all at P＜0.05).EPQ data showed that the scores of psychology and nervousness in the POAG group and the PACG group were higher than those in the normal control group (all at P＜0.05),but no significant difference were found in extroversion and stability of the personality among the three groups (P=0.321,0.315).The total scores of SAS and SDS were considerably higher in the POAG group and the PACG group compared with the normal control group or the PACG group compared with the POAG group (all at P＜0.05).The incidences of anxiety and depression were elevated in the POAG group and the PACG group in comparison with the normal control group (x2 =15.520,P =0.000 ; x2 =14.870,P =0.000).Conclusions The personality and behavioral factors are associated with the pathogenesis,development and therapy of primary glaucoma.The anxiety,depression and stronger stress response are found in glaucoma patients,especially PACG patients.

Objective To investigate the influences of Tangbikang on the oxidative stress in rats with diabetic penipheral neuropathy (DPN). Methods Diabetes rats were induced by intraperitoneal injection of STZ, 55 mg/kg and randomly divided into five groups: model group, methycobal treatment group, and Tang Bi Kang low, medium and high dosage treatment group. Body weight was examined every week. All rats were sacrificed at week 8 and the values of blood serum, superoxide dismutase, malondialdehyde, nitric oxide synthase and nitric oxide were detected. Results After treated with Tang Bi Kang, the eighth week's body weight, the levels of SOD, NOS and NO in the blood serum and ATP of sciatic nerve were increased greatly (P＜0.01 or P＜0.05) and the contents of MDA and AGEs and AR of sciatic nerve were decreased (P＜0.01), compared with the model group. Conclusion Tang Bi Kang may lighten the oxidative stress reaction of diabetic penipheral neuropathy and slow down the course of the diabetic penipheral neuropathy.

Purpose To investigate the effects of oxytocin(OT) and prostaglandins intervention on oxytocin receptor(OTR) expression of primary culture of human myometrial smooth muscle cell.Methods Using respectively oxytocin, prostaglandin E2(PGE2),and prostaglandin F2alpha(PGF2alpha) intervened on human myometrial smooth muscle cell.The expression of OTR mRNA and protein of cell hemogenate was examined.Results The expression of OTR between oxytocin intervention group and untreated group was similar.The expression of OTR in cell was significantly higher in the PGE2 or PGF2alpha intervention group than that in the untreated group.The expression of OTR in cell was significantly higher in the PGE2 and PGF2alpha joint intervention group than that in the untreated group and than that in the PGE2 or PGF2alpha individual intervention group.Conclusion Oxytocin didn′t increse the expression of OTR in human myometrial smooth muscle cell.PGE2 and PGF2alpha incresed the expression of oxytocin receptor in human myometrial smooth muscle cell.Furthermore PGE2 and PGF2alpha joint intervention more significantly increased the expression of oxytocin receptor than PGE2 or PGF2alpha individual intervention in human myometrial smooth muscle cell.