BACKGROUND:Studies have reported that underin vitro experimental environment, antisense endothelin nucleic acid nanometer carrier can express target nucleic acid and produce RNA interference effect after enfolding by 12-alkylated chitosan nanoparticles, which can effectively inhibit the excessive generation of endothelin from inflammatory cytokines induced by alergen. OBJECTIVE:To investigate the effect of 12-alkylated chitosan nanoparticles enfolding antisense endothelin converting enzyme RNA expression plasmid on airway hyperresponsiveness in asthmatic mouse models. METHODS: Forty Balb/c mice were randomly divided into four groups: control, chitosan nanoparticles, normal saline and plasmid groups. Mice in the chitosan nanoparticles, normal saline and plasmid groups were subjected to sensitization by an intraperitoneal injection with ovalbumin (0, 14 days) and motivation by aerosol inhalation of ovalbumin (24, 25, 26 days) to induce asthma models. Mice in the control group were subjected to sensitization and motivation by the perfusion of normal saline. At 24hours before the first excitation, mice in the control, chitosan nanoparticles, normal saline and plasmid groups were perfused with normal salinevia airway, 12-alkylated chitosan nanoparticles, normal saline and antisense endothelin converting enzyme RNA. At 48 hours after the last excitation, the airway reactivity of mice was detected. After 28 days, bronchoalveolar lavage fluid cytology, lung histopathology, cytokines in spleen cel culture supernatant were detected. RESULTS AND CONCLUSION:Compared with the control group, the total number of plasmid cels, the percentage of eosinophils, eosinophil absolute counts, interleukin-4 levels, endothelin levels and airway hyperresponsiveness of mice in chitosan nanoparticles, normal saline and plasmid groups were increased (P < 0.05), and the lung inflammation was more severe. These indicators in the chitosan nanoparticles group were al lower than those in the normal saline and plasmid groups (P < 0.05) and the degree of inflammation was lighter than that in the saline and plasmid groups. These results demonstrate that 12-alkylated chitosan nanoparticles enfolding antisense endothelin converting enzyme RNA expression plasmid can reduce the synthetic amount of asthma endothelin and inhibit airway responsiveness.

BACKGROUND:Pancreas or islet cel transplantation and stem cel transplantation bring hope to cure diabetes, but pancreas or islet transplantation appears to have a lack of donors as wel as immune rejection problems, limiting their clinical development. Therefore, stem cel transplantation therapy has become the current hotspot.
OBJECTIVE:To study the therapeutic effects of huaman telomerase reverse transcriptase (hTERT)-modified bone marrow mesenchymal stem cel s transplantation on diabetes mel itus in SD rats.
METHODS:Bone marrow mesenchymal stem cel s were transfected with PLXSN carrying hTERT. Thirty-six male SD rats were randomly divided into control group (n=6), stem cel group (n=10), hTERT transfection group (n=10), diabetes mel itus group (n=10). Except the control group, the rats were injected with stretozotocin (45 mg/kg) to make diabetes mel itus models. After modeling, rats in the stem cel group and hTERT transfection group were respectively intravenously injected with 1 mL of bone marrow mesenchymal stem cel s (1.5×1010/L) and 1 mL of hTERT-modified bone marrow mesenchymal stem cel s (1.5×1010/L).
RESULTS AND CONCLUSION:At 24 hours after modeling, the fasting blood-glucose level was significantly increased in the diabetes mel itus group, which was higher than the normal value (6.7 mmol/L). At 15 days after cel transplantation, the fasting blood-glucose levels were signficiantly decreased in the stem cel group and hTERT transfection group as compared with the diabetes mel itus group (P<0.05), but the body mass of rats was increased in these two group (P<0.05), especial y in the hTERT transfection group. At 45 days after cel transplantation, the fasting blood-glucose level and body mass in the stem cel group and hTERT transfection group were close to those in the control group (P>0.05), and moreover, the hTERT group had better outcomes than the stem cel group. Meanwhile, in the diabetes mel itus group, the fasting blood-glucose level was stil at a higher level, and the body mass decreased continously. These findings suggest that hTERT-modified bone marrow mesenchymal stem cel transplantation is effective for treatment of diabetes mel itus in rats.

Primary liver cancer ( PLC) is one of the most invasive malignant tumors in the clinics .The PLC within Milan criteria can be cured by liver transplantation or liver resection .However,PLC exceeding Milan criteria has been a question for us to overcome all the time .In recent years ,transcatheter arterial chemoemboliza-tion( TACE) and its combination therapy for PLC exceeding Milan criteria have made some breakthrough .A great deal of literatures have confirmed that TACE and its combined therapy for PLC exceeding Milan criteria can im -prove the survival rate obviously,it also contains many advantages,such as low cost,safe,painless,minimally in-vasive,repetitive operation and so on .The progress of TACE for PLC exceeding Milan criteria in recent years is summarized in this paper .

Clinically, chronic obstructive pulmonary disease (COPD) and coronary heart disease (CAD) often interact to promote disease progression. This article discussed that zongqi is the heart-lung correlation functional basis and yingwei relevant is heart-lung correlation core. Zongqi deficiency is the incidence basis of COPD and CAD from the pathology. Communication and transformation of nutrient and defensive qi is the core mechanism of COPD combined with CAD. Phlegm and blood stasis are toxic intricate. Further damage to jingqi is the key to combination of COPD and CAD. It lays the foundation for next clinical syndrome differentiation, development of comprehensive treatment programs and clinical research.

Inhibin B(INHB) and anti-Müllerian hormone(AMH) detection have important clinical significance in assisted reproductive technology.INHB for evaluation of male testis function is very meaningful,playing a guiding role in the treatment of oligospermatism.INHB has very high clinical diagnosis value for evaluation of ovarian reserve function,being an important indicator to predict the effect of controlled ovarian hyperstimulation.AMH for evaluation of women ovarian function is significant,and is a diagnosis index of reproductive domain related diseases such as polycystic ovary syndrome and one of the important indices for evaluation of assisted reproductive outcomes.

Objective To study the changes of mean platelet volume and urinary albumin excretion rate and retinal thickness and relationship among them in diabetes mellitus patients with retinopathy(DR).Methods Mean platelet volume was measured with MEK-6318K automated blood cell counter;retinal thickness was measured with optical coherence tomography(OCT);urinary albumin excretion rate was measured with rate nephelometry in DR,which were compared with those of 30 normal controls.Results The mean platelet volume and retinal thickness and urinary albumin excretion rate in DR were significantly higher than those in normal controls(P

Objective To compare cerebral oximetry(rSO 2 %)and somatosensory evoked potential (N 35 ) in their accuracy in monitoring cerebral perfusion during carotid endarterectomy procedure under general anesthesia. Methods Ten patients (6 males, 4 females), scheduled for selective carotid endarterectomy, were enrolled in the study. Their rSO 2 %and N 35 were also continuously monitored and recorded during perioperative periods. The patients' cognitive function was evaluated at the same time. The MAP, HR, SpO 2 were continuously monitored during operation. Results There was a significant difference in rSO 2 % between that at the period of elamping and that at the period of declamping of the carotid artery. No patients suffered from cognition dysfunction during perioperative period. Conclusion With comparison to the amplitude of N 35 it was rSO 2 % which was earlier to show alteration in cerebral perfusion in case of cerebral oxygen deficit. In monitoring cerebral perfusion, rSO 2 % was better than N 35

Objectives: To study the correlation between the dynamic expression of aquaporin-4 (AQP-4) around hematoma and the blood-brain barrier permeability after intracerebral hemorrhage in rats. Methods: Forty-two Sprague-Dawley rats were randomly allocated into sham-operation group, and groups of 6 hours, 12 hours, 1, 3, 7 and 14 days (n=6 in each group) after intracerebral hemorrhage. The intracerebral hemorrhage model in rats was established by injecting autologous arterial blood into caudate nucleus. Immunohistochemical method and Evans blue method were used respectively to detect the dynamic changes of AQP-4 around hematoma and the blood-brain barrier permeability in different time periods after intracerebral hemorrhage in rats. Results: (1) The expression of AQP-4 around hematoma increased gradually 12 hours after intracerebral hemorrhage, it increased significantly at day 1 and reached a maximum at day 3. It was slightly higher than normal level at day 7 and almost returned to normal at day 14 (P

Objective To investigate the correlation analysis of amplitude-integrated electroencephalogram (aEEG) and magnetic resonance imaging (MRI) in infants with hypoxic-ischemic encephalopathy (HIE) to early evaluate the severity of HIE.Methods Ninety term infants with HIE were selected.They were detected by aEEG within 12 hours after birth,and accepted cranial MRI 3 to 7 days after birth.The infants were divided into 3 groups (aEEG normal,mild abnormality and severe abnormality) according to the result of aEEG.The Spearman correlation analysis was completed in infants with HIE between aEEG clinical grading and cranial MRI grading.Results In 90 infants with HIE,the clinical grading:mild HIE 44 cases (48.9％),midrange HiE 29 cases (32.2％) and severe HIE 17 cases (18.9％).Cranial MRI grading:mild 49 cases (54.4％),midrange 23 cases (25.6％) and severe 18 cases (20.0％).The result of aEEG:aEEG normal 43 cases (47.8％),aEEG mild abnormality 25 cases (27.8％) and aEEG sever abnormality 22 cases (24.4％).The result of correlation analysis showed that aEEG grading was positive correlation with the HIE clinical grading (r =0.970 7,P ＜ 0.01) and MRI grading (r=0.933 5,P ＜ 0.01).Conclusion The aEEG can early evaluate the severity of HIE.

Objective To analyze the value of carbon nanoparticles in the cleaning of thyroid cancer lymph node.Methods 80 patients with thyroid cancer from March 2012 to March 2013 were included in this study.Based on the sequence of enrollment,they were randomly divided into two groups:the therapy group (n=40)were received Nano carbon lymphatic tracer before basic operative treatment and the control group (n=40)were directly received the same basic operative treatment.The liver function parameters in two groups were observed and compared statistically. Results The operating time,operative blood loss and postoperative drainage volume had no statistically significant difference between two groups.The incidence of postoperative hypocalcemia in experimental group was lower than that in blank control group(P<0.05 ).The parathyroid hormone level in experimental group on the first postoperative day was higher than that in blank control group(P<0.05 ).The number of resected lymph nodes in experimental group was more than that in blank control group(P<0.01).Conclusion Nano carbon lymphatic tracer technique can increase the cleaning number of lymph node of thyroid cancer patients in central region,and realize the accurating tumor stage.