Animals ; Animals, Newborn ; Cell Survival ; drug effects ; Cells, Cultured ; Cerebral Cortex ; cytology ; drug effects ; Dose-Response Relationship, Drug ; Neurites ; drug effects ; Neurons ; cytology ; drug effects ; Organometallic Compounds ; toxicity ; Rats ; Rats, Wistar

OBJECTIVETo observe the clinical efficacy on fibromyalgia syndrome treated with acupuncture at the acupoints selected from the affected meridians based on the location differentiation and the heavy moxibustion at painful points by the randomized controlled trial with medication.

METHODSThirty-two patients of fibromyalgia were randomized into an acupuncture-moxihustion group and a medication group, 16 cases in each one. In the acupuncture-moxibustion group, acupuncture at the acupoints selected from the affected meridians based on the location differentiation and the heavy moxibustion at painful points were applied, once every two days, three times a week, for 4 weeks totally. In the medication group, tramadol sustained release tablets and amitriptyline were prescribed for oral administration, once a day, for 4 weeks. The follow-up visit was conducted for 4 weeks in the two groups. Before and after treatment and in follow-up visit, the revised version of the fibromyalgia impact questionnaire (FIQR) and visual analogue scale (VAS) were adopted for assessment and comparison of the efficacy between the two groups.

RESULTSAfter treatment and in follow-up visit, FIQR score and VAS score were improved apparently as compared with those before treatment in the two groups (all P<0. 05). At the end of treatment, the differences were not significant statistically between the two groups (all P>0. 05). But the differences were significant in follow-up visit (all P<0. 05). The scores in the acupuncture-moxibustion group were improved persistently, but those in the medication group were rebound.

CONCLUSIONThe acupuncture at the acupoints selected from the affected meridians based on the location differentiation and the heavy moxibustion at painful points are safe and effective in the treatment of fibromyalgia syndrome and present the better persistent effect as compared with the combined medication of tramadol sustained release tablets and amitriptyline.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Female ; Fibromyalgia ; therapy ; Humans ; Male ; Meridians ; Middle Aged ; Moxibustion ; Pain Measurement ; Young Adult

Objective To evaluate the regulatory effect of hydroxychloroquine on in vitro differentiation of regulatory T cells(Treg). Methods Fifteen milliliters of peripheral blood were obtained from a healthy human subject followed by isolation of monouclear cells and sorting of naive T cells. Then, the naive T cells were classified into two groups:control group cultured with the presence of tumor growth factor(TGF)?βand interleukin(IL)?2, experimental group cultured with the presence of 20μmol/L hydroxychloroquine and inducing factors. The number and percentage of CD4+Foxp3+Treg cells were determined by flow cytometry at days 0, 6, and 12. Results The number of Treg cells at days 0, 6, and 12 were(2 ± 0.4)× 104,(13.2 ± 5.2)× 104, and(143.5 ± 35.9)× 104 respectively in the control group, (2 ± 0.5)× 104,(2.4 ± 0.4)× 104, and(5.6 ± 3.5)× 104 respectively in the experimental group. There was a significant difference in the number of CD4+Foxp3+ Treg cells between the two groups at days 6 and 12 (t = 3.78 and 6.16, respectively, both P < 0.05). At day 12, the percentage of CD4 +Foxp3 + Treg cells was 79.7% ± 18.1% in the experimental group, compared to 16%± 13%in the control group(t=11.77, P<0.01). Conclusion Hydroxychloro?quine could inhibit the differentiation and proliferation of CD4+Foxp3+Treg cells in vitro.

Objective The study aimed to improve the medication safety of aged patients through the reengineering of the dispense process of discharge medications for elderly cardiac patients.Methods The original dispense process was analyzed to find the existing problems,changing the order of process,increasing or decomposing steps of process in order to redesign,perfect and implement the distribution process of medications.Time and correction rate of drug dispensing for three times a day,mastering degree of related knowledge of oral medication and satisfaction degree with medication instruction were compared before and after reengineering of the process.Results Compared with the original process,patients' ability to understand the drug knowledge,the timing when the patients take three medications per day and the satisfaction degree with the instructions of taking medications all had statistical significance.Conclusions Reengineering of the dispense process of discharge medications for elderly cardiac patients can help to improve patients' understanding of drug knowledge,improve the security of taking medications outside the hospital as well as their satisfaction degree with nursing.

Objective:To discuss the relation among t-PA,PAI,unstable angina risk strati cation and its TCM syndrome.Methods:Syndrome di erentiation,coronary angiography and risk strati cation for 86 cases of unstable angina pectoris were carried out,and Gensini scoring system was used to assess the results of coronary angiography,blood stasis score was used to assess the degree of blood stasis.Serum blood serum t-PA and PAI levels were detected for statistical analysis.Results:In 86 cases of patients with low-risk,middle-risk and high-risk groups,with the incremental risk strati cation,blood stasis and Gensini score were increased,by comparison among the three groups,the di erences was existed(P

The aim of the study was to explore the role of NF ?B activation in the pathogenesis of smoke inhalation injury and to evaluate the effects of dexamethasone on NF ?B activation. 130 Wistar rats were inflicted with smoke inhalation injury and randomized equally into the control group, smoke inhalation injury group and dexamethasone treatment group.The expressions of NF ?B P 65 ,I?B ?,TNF ?,and ICAM 1 proteins in the lung tissue were determined by immunohistochemistry at 2,6,12,24,48,72 hours after smoke inhalation injury. The results showed that,after smoke inhalation ,the expressions of NF ?BP 65 ,TNF ?,and ICAM 1 increased,whereas the expression of I?B ? decreased. In the dexamethasone treatment group,the expressions of NF ?BP 65 ,TNF ?,and ICAM 1 were down regulated, and I?B ? was up regulated.These results suggest that NF ?B activation may participate in the pathogenesis of smoke inhalation injury.Dexamethasone could suppress NF ?B activation, thus partially blocked the production of cytokines and adhesion molecules,and in turn reduced the damage of inflammatory response. Therefore NF ?B activation might be a key point in the development of smoke inhalation injury and modulation of activation of NF?B might be a potential therapeutic strategy to treat this injury at the transcription level.

Measures were taken to reform the current teaching mode including establishing cur-riculum system which embodies medical characteristics,exploring probation mode suitable for local med-ical college,setting up students' autonomous learning platform,improving performance evaluation meth-ods,teaching students in accordance with their aptitude,etc. Meanwhile,new partnership between school and hospital was explored though improving teaching management system. After taking above measures, students' practical ability was significantly enhanced and quality of teaching was improved.

Luteinized unruptured follicle syndrome (LUFS), a specific type of ovulation disorder, is for a cause of female infertility. Acupuncture therapy has a good effect for LUFS. In order to understand the clinical effects and research situation of acupuncture therapy for LUFS, the author analyzed and reviewed the relevant literature. It was indicated by the findings that acupuncture therapy and integrative therapy of acupuncture and Chinese herbs have good therapeutic effect for this disease. Acupuncture therapy has the effect to promote ovulation. By the therapeutic principle to tonify the kidney and circulate blood, acupuncture therapy mostly was adopted in the period of ovulation. The acupoints are mostly selected from the lower abdomen and lower limbs, possibly based upon syndrome differentiation at the same time. The needling techniques are of certain characteristics. But, the issues of low quality, un-standardized inclusive, exclusive criteria and effective criteria still exist in the current clinical study.

Objective To observe whether peripheral blood mononuclear cells (PBMCs) from patients with active systemic lupus erythematosus (SLE) can induce the adhesion of T lymphocytes to endothelial cells. Methods Sera and PBMCs were obtained from patients with active SLE and normal human controls. PBMCs were cultivated and culture supematants were harvested. Human umbilical vein endothelial cells (HUVECs) were cultured in vitro with or without the presence of the sera or culture super-natants of PBMCs. Some cells were pretreated with the antibody to IL-17 before the treatment with the sera or supematants. After another 48-hour culture, RT-PCR and real-time PCR were used to detect the mRNA expressions of adhesion molecules, including intercellular adhesion moleeule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and E-cadherin in HUVECs, wound healing assay to estimate the motility of HUVECs. Additionally, T lymphocytes were added to HUVECs 48 hours after stimulation with the sera or supematants, the adhering of T lymphocytes to HUVECs was observed by microscopy. Results After stimulation with supematants of PBMCs from patients with active SLE, the mRNA expressions of ICAM-1, VCAM-1 and E-cadherin were significantly increased in HUVEC, while the increase could be inhibited by the antibody to IL-17. The elevation of adhesion molecule expression subsequently promoted the motility of HUVEC, mediated the adhesion of T lymphocytes to HUVEC, and the antibody to IL-17 could suppress the adhesion of T lymphocytes and motility of HUVEC. Conclusion The culture supematants of PBMCs from patients with active SLE can induce the expression of vascular cell adherin molecules and promote the adherin of T lymphocytes, which may in turn mediate the development of lupus vasculitis.

Aim To investige the protective effect of FMCL on the apoptosis of PC12 cells induced by H_2O_2 and its mechanisms.Methods Apoptosis of PC12 cells induced by H_2O_2 at the concentration of 0.5 mmol?L~(-1).The cell activity was determined by MTT.The morpholog changes were observed by invert microscope.DNA ladder was observed through agarose gel electrophoresis.The subdiploid peaks showing cell apoptosis rate and △?m were detected by flow cytometry.Results Compared with those of the Model group the cell activity of FMCL 100、30 mg?L~(-1) group reinforced,the injury of the cell relieved.The apoptosis rate of those two groups decreased,but the△?m and the cell activity increased in a dose-dependent manner.Conclusion FMCL can inhibit the apoptosis of PC12 induced by H_2O_2 which might be correlated with the increasion of △?m.