1.Community Structure and Ecological Distribution of Airborne Microbes in Autumn in Xi'an
Yi-Ping CHEN ; Ying CUI ; Jun-Ji CAO ;
Journal of Environment and Health 2007;0(10):-
Objective To understand the air microorganisms pollution and the community structure and distribution characteristics of outdoor airborne microbes in Xi'an in autumn.Methods Airborne microbes were sampled by fallen plates method in autumn,2006 in Xi'an at three typical sites,factories region,garden greenbelt region and main traffic region.Results The results showed that the total concentration of airborne microbes at the factories region and garden greenbelt region,main traffic region was 2 500 cfu/m~3,1 354 cfu/m~3 and 4 404 cfu/m~3 respectively.Bacteria and fungal genus were predominant.Gram positive bacteria were much more than Gram negative bacteria.There were 20 genera of bacteria and 8 fungal genus were detected. Micrococcui,Bacillus,Staphylococcus and Pseudornonus were predominant.The most frequently isolated fungal genus were Penicillium,Paecilomces,Cladosporium and Nonsporulatiny mycelia.The community structure of airborne fungal genus and the dominant genus in different function region was significantly different.The distribution characteristic of airborne microbe concentration in different function region was different.The concentration of airborne bacteria was significantly lower in garden greenbelt region than factories region and main traffic region,whereas the concentration of airborne fungi in garden greenbelt region was higher than that in factories region and main traffic region.Conclusion The distribution of the airbome microbes shows a characteristic of time and space,it is affected not only by the environment and pollution,but also by the other factors such as human activities,animals and plants.
2.Expression of survivin and P53 detected by tissue microarray and its significance in gastric adenocarcinoma
Qi-Chang YANG ; Yi-Zhong FENG ; Chong-Jun ZHONG ; Ping WANG ; Xiaohua JI ; Yi SHEN ;
Chinese Journal of Digestion 2001;0(11):-
Objective To investigate the role of survivin and P53 in apoptosis of gastric adeno- carcinoma,as well as its relationship with the clinicopathologic features and the prognosis.Methods The gas- tric tissue microarrays were composed of those from 100 cases of gastric cancer and 30 controls.At these tissue microarrays,expressions of survivin and P53 were investigated immunohistochemically,and tumor cell apoptosis index was examined by TUNEL method.Of the 100 cases,47 cases were followed-up from 14 months to 13 years,in which the survival was analyzed.Results Two paraffin-embedded gastric carcinoma tissue micro- arrays were successfully constructed,including 114 and 116 tissue spots,respectively.Immunohistochemical analysis showed that survivin was expressed in 78 cases (78%).No expression of survivin was detected in control tissue (P0.05).In the 47 cases with followed-up data,univariant analysis revealed that the survival was correlated with invading of vessel and nerve,TNM stages,and expression of survivin.The histological grades and expression of P53 were not related to prognosis.However,Cox stepwise proportional hazards analysis showed that only TNM staging and survivin status retained significant independently in prospecting prognosis.Conclusions The expression of survivin was associated with the pathologic features,TNM stages and prognosis in gastric carcinoma,indicating that overex- pression of survivin may be a poor prognosis factor for gastric carcinoma.
3.Detection technologies of microRNA and their prospects for forensic applications.
Zheng WANG ; Ji ZHANG ; Dan-Zhou TANG ; Yan-Bin WANG ; Yi-Ping HOU
Journal of Forensic Medicine 2014;30(1):55-59
MicroRNA (miRNA) belongs to a class of small, non-coding RNA molecules that contains 18-25 nucleotides and regulates gene expression at post-transcriptional level. Many miRNAs are highly conserved and display timing- and tissue-specific expression. With the advance of the miRNA detection technologies, miRNA has been introduced to forensic science as a potentially novel set of genetic markers of forensic body fluid identification, species identification and PMI estimation. In this article, the detection methodologies of miRNA are reviewed, and their potential applications in forensic practice and research future are also discussed.
Body Fluids
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Forensic Medicine
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Genetic Markers
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Humans
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MicroRNAs
4.To Strengthen Teaching Quality of Otorhinolaryngological Advanced Students
Ping LV ; Ji WU ; Siquan TANG ; Tianming ZHOU ; Hongying PU ; Yi WANG
Chinese Journal of Medical Education Research 2006;0(07):-
The teaching for medical advanced students is a routine and important task in teaching hospital.To increase the teaching quality,we should emphasize professional theory learning,increase clinical skill training,strengthen medical records writing training and enhance emotional exchange.
5.In vitro and in vivo effect of recombinant human growth hormone on the growth of gastric cancer cells
Jiayong CHEN ; Daoming LIANG ; Ping GAN ; Ji LIN ; Feng LIN ; Yi ZHANG
Chinese Journal of General Surgery 2000;0(12):-
0 05)in all parameters The tumor inhibitory rate and the cells detained in G 0~G 1 phase significantly increased, meanwhile, cells in S phase, the PI and PCNA significantly decreased in rhGH+L OHP group compared with control group or rhGH group ( P
6.Influence of Excessive Na Stress on Growth and Element Accumulation of Three Species of Ectomycorrhizal Fungi
Yi HUANG ; Xue-Yan JIANG ; Hai-Bo JI ; Tian-Ping XU ;
Microbiology 1992;0(03):-
This study deals with colony expansion characteristics, biomass increment and Na accumulation in mycelia of three ectomycorrhizal fungi ( Boletus edulis, Xerocomus chrysenteron and Gomphidius viscidus ) under treatment of Na2SO4 and NaCl. The experimental results showed that no impact was observed from Na2SO4 and NaCl treatments on the growth mode of the mycelia, but the biomass increment of X. chrysenteron and G. viscidus was significantly restrained under 0.1 mol/L Nacl. Under the treatment of Na2SO4, biomass increment of X. chrysenteron is significantly higher than the control, but the biomass of G. viscidus is lower than that of the control, and no significant impact was measured on growth of B. edulis under both treatments of Na2 SO4 and NaCl. The experiment also indicated that Na accumulation in mycelia varied significantly among the three tested strains, highest Na accumulation was measured in X. chrysenteron under treatment of NaCl, while under the treatment of Na2SO4 , accumulation of Na in B. edulis is much higher than the other two strains.
7.Progress on the pathogenesis and treatment of IgG4-related disease
Xing JI ; Chen-yang LU ; Ping-ying QING ; Yi ZHAO ; Yi LIU ; Chun-yu TAN ; Yu-bin LUO
Acta Pharmaceutica Sinica 2022;57(8):2283-2291
IgG4-related disease (IgG4-RD) is a newly recognized fibro-inflammatory condition of autoimmune etiology in recent twenty years, mainly manifesting as mass-forming lesions in single or multiple organs. In the past, it was often missed or misdiagnosed as inflammation or tumor. Patients may die from multiple organ failure due to end-stage fibrosis if they are not treated promptly. However, the number of clinically confirmed cases has gradually increased with the improvement of diagnostic level in recent years, and these patients have benefited greatly after receiving early treatment. Although patients generally respond well to traditional immunosuppressors including glucocorticoids and disease-modifying anti-rheumatic drugs, refractory and recurrent cases, even patients with glucocorticoid contraindication are common. Important mechanistic insights have been derived from studies of B-cell depletion therapy, but greater awareness of the pathophysiology of IgG4-RD is still badly needed to identify novel therapeutic targets. In this article, we reviewed the pathogenesis progress and promising therapy of IgG4-RD to seek better clinical management of IgG4-RD.
8.Establishment of a rat silicosis model via non-exposed tracheal instillation in combination with a ventilator
China Occupational Medicine 2022;49(05):548-
Objective -
To establish a new non exposed intratracheal instillation method for establishing a rat silicosis model.
Methods ,
The specific pathogen free SD rats were randomly divided into control group and experimental group with ten rats in
,
each group. Rats in the control group were given 1.0 mL of 0.9% sodium chloride solution and rats in the experimental group
-
were given 1.0 mL of silica suspension with a mass concentration of 50 g/L adopting to the one time intratracheal instillation
, - ,
method and then followed by ventilator assisted ventilation immediately. When the tidal volume stabilized at 2.0 mL the
ventilator was removed and the tracheal intubation was pulled out. Five rats in each group were sacrificed after two and four
, - Results
weeks after modeling and hematoxylin eosin staining and Masson staining of lung tissue were performed. There was
, ,
no death in the two groups of rats during the experiment. After two and four weeks the control group had normal lung structure
, , ,
normal alveolar cavity size no inflammatory cell infiltration thin alveolar wall only a small amount of collagen distribution
,
around the lung interstitium and bronchus. At the second week of modeling the alveolar wall of the rats in the experimental
, , ,
group was slightly thickened interstitial lymphocytes and macrophages were infiltrated slight hyperplasia was found and a
,
small amount of fibroblasts were visible. At the 4th week of modeling the alveolar wall of the rats in the experimental group was
, , , ,
significantly thickened fibrous nodules were formed and fibroblasts fibrocytes collagen fibers were significantly increased.
Conclusion -
The combination of ventilator and non exposed intratracheal instillation method can be used to successfully
, , .
establish a rat silicosis model which is simple safe and effective
9.Application of hydrogen proton magnetic resonance spectroscopy in diagnosis of peripheral tumor cell infiltration of gliomas
Yi-ling, FAN ; Yong, WANG ; Jie-qing, WAN ; Zheng-wen, ZHOU ; Ji-yao, JIANG ; Jia-lin, SHENG ; Fei-ping, LI
Journal of Shanghai Jiaotong University(Medical Science) 2009;29(7):854-857
Objective To explore the application of hydrogen proton magnetic resonance spectroscopy (1H-MRS) in the diagnosis of peripheral tumor cell infiltration of gliomas. Methods Forty patients with glioma were examined by 1H-MRS preoperation, and were divided into low grade glioma group (n=20) and high grade glioma group (n=20) according to postoperative pathological diagnosis. Tumor resection with peripheral tissues marked previously was carried out under the guidance of neuronavigator system. All the pathological sections were divided into positive group and negative group according to the presence or absence of tumor cells, and the differences in pathological findings of peripheral regions (region 1, 2 and 3) and 1H-MRS values were analyzed in these two groups. Results No infiltration was found in the peripheral regions of low grade glioma group except for one case in peripheral region 1, while infiltration was found in all peripheral regions of high grade glioma group. There was no significant difference in 1H-MRS values between positive group (n=24) and negative group (n=36) in patients with high grade glioma (P>0.05). Conclusion 1H-MRS enjoys some advantages over routine radiological examinations in the diagnosis of peripheral tumor cell infiltration of gliomas. Total removal can be expected when combined with neuronavigator system, while there is room for improvement for relevant techniques.
10.Effects of HO-1 on Lipopolysaccharide-induced Endoplasmic Reticulum Stress of Rat Hepatocytes.
Yan-sha WANG ; Ying-lei JI ; Tao WANG ; Lin-lin WU ; Cheng-ping FEI ; Yi-chang LIU ; Zhen-yong GU
Journal of Forensic Medicine 2015;31(6):417-421
OBJECTIVE:
To investigate effects of antioxidant stress protein heme oxygenase-1 (HO-1) on lipopolysaccharide (LPS)-induced endoplasmic reticulum stress (ERS) of rat hepatocytes.
METHODS:
The BRL cells (rat hepatocyte cell line) were cultured. The hepatocytes were treated with LPS, LPS+HO-1 siRNA, HO-1 siRNA and PBS solution, respectively. The cell viability was measured by trypan blue exclusion test. The apoptosis cells were detected by the fluorescent dye Hoechst 33258. Expressions of GRP78, CHOP, caspase-12 and HO-1 were detected by Western blotting.
RESULTS:
LPS caused an increase of HO-1 protein expression of rat hepatocytes in a dose-dependent and time-dependent manner, a up-regulation of GRP78, CHOP and caspase-12, a decrease in cell viability, and an increase in apoptosis rate of hepatocytes. Pretreatment of HO-1 siRNA inhibited the up-regulation of LPS-induced HO-1, however, aggravated ERS and cellular injury.
CONCLUSION
HO-1 inhibites ERS-mediated cellular injury of rat hepatocytes induced by LPS.
Animals
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Apoptosis/physiology*
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Endoplasmic Reticulum/metabolism*
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Endoplasmic Reticulum Stress/physiology*
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Heme Oxygenase-1/pharmacology*
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Hepatocytes/metabolism*
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Lipopolysaccharides/pharmacology*
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Rats