Objective To explore the mechanisms of TGF-? 1?TGF-? 1RⅡ and NF-?B on the angiogenesis in patients with hepatocellular carcinoma.Methods The expression of TGF-? 1 ?TGF-? RⅡ and NF-?B protein in 36 cases of HCC and surrounding HCC tissue was separately detected using immunohistochemistry technique.To observe the relationship of TGF-? 1 protein and MVD, TGF-? 1RⅡ protein and MVD, NF-?B and TGF-? 1 protein, using CD34 labelling vessel endothelial cell. Results The expression of TGF-? 1 and MVD in HCC tissue was higher than that in surrounding HCC tissue (P

Objective To observe the effects of intranasal dexmedetomidine (DM)as premedi-cation on postoperative behavioral outcomes in children.Methods Sixty ASA physical status Ⅰ or Ⅱchildren of both genders,2-5 years,weighing 10-30 kg,undergoing hernia surgery,were equally as-signed into three groups (n =20 each)using a random number table:control group (group C),mid-azolam group(group M)and dexmedetomidine group(group D).Thirty minutes before anesthesia in-duction,the children were respectively received intranasal normal saline 0.02 ml/kg (group C),in-tranasal midazolam 0.2 mg/kg (group M)and intranasal DM 2 μg/kg (group D).The sedation score of children apart from parents,the receipt score of face mask for sevoflurane anesthesia induction,the postoperative recovery time,adverse effects,and the percentage of patients requiring rescue analgesic were recorded.To observe the postoperative behavioral outcomes on 1th、7th、30th day using the PH-BQ.Results Compared with group C,the sedation score and the receipt score of face mask of groups M and D were significantly increased (P <0.05).Compared with groups C and M,the adverse effects and the percentage of patients requiring rescue analgesic of group D were decreased (P <0.05).The incidence of the postoperative behavioral outcomes of group C was higher than groups M and D on 1th,7th day (P <0.05).And on 30th day after operation,there was no significant difference among three groups.Conclusion Intranasal dexmedetomidine as premedication can significantly decrease the incidence of the postoperative behavioral outcomes in children.

Background and purpose:In recent years,sleeve resection of bronchus and pulmonary artery has been more extensively applied in treatments of lung cancer patients with poor pulmonary functions.This kind of surgery meets the basic surgical principles of lung cancer being maximum both in resecting the carcinoma of the lungs and preserving pulmonary functions,has a good prospect.Therefore,we summarize our recent experience in treating central type lung cancer by this method and discuss its principles and methods in the application of the surgical operation.Methods:From Dec.1993 to Dec.2003,63 patients with central type lung cancer underwent sleeve lobectomy of bronchus and pulmonary artery,including sleeve resection of bronchus and pulmonary artery in 43 cases,wedge resection of bronchus and pulmonary artery in 3 cases,sleeve resection of bronchus and wedge resection of pulmonary artery in 17 cases.The surgical procedures of sleeve resection included sleeve resection of right upper lobe and pulmonary artery in 20 cases,sleeve resection of left upper lobe and pulmonary artery in 23 cases,and the length of pulmonary artery that had been respected is from 1.5cm to(4.5cm),the average length is 2.5cm.The surgical procedures of wedge resection included wedge resection of right upper lobe and pulmonary artery in 2 cases, wedge resection of left upper lobe and pulmonary artery in 1 case.The group of sleeve resection of bronchus with wedge resection of pulmonary artery included sleeve resection of right upper lobe and wedge resection of pulmonary artery in 5 cases,sleeve resection of left upper lobe and wedge resection of pulmonary artery in 12 cases.Results:No case of death and anastomotic leak occurred.Conclusions:Because sleeve resection of bronchus and pulmonary artery can not only effectively resect the tumor but also maximally preserve the pulmonary function at the same time,it enlarges the surgical indications of lung cancer,and is an effective and safe method for the treatment of lung cancer.

Objective:To analyse the quality control assessment results of arsenic testing laboratories of national endemic disease prevention and control institutions from 2006 to 2020, so as to further strengthen the quality control of arsenic determination in laboratories of national endemic disease prevention and control institutions, and to ensure the accuracy and reliability of national endemic arsenic poisoning monitoring data.Methods:The water arsenic and urinary arsenic test results of arsenic quality control laboratories from 2006 to 2020 were collected and evaluated by Z-ratio scoring method(│Z│≤2 was qualified, 2 < │Z│ < 3 was basically qualified, │Z│≥3 was unqualified), and the qualification rates of water arsenic and urinary arsenic in different years and different provinces were calculated.Results:From 2006 to 2020, the number of laboratories participating in water arsenic quality control in 14 provinces has increased from 25 to 167, and the number of laboratories participating in urinary arsenic quality control increased from 22 to 90. The standard deviation of laboratory assessment samples was relatively stable, all < 0.05 mg/L. The quality control assessment results from 2006 to 2020 were calculated according to │Z│ < 3, the qualified rate of water arsenic ranged from 64.0% to 100.0%, and that in urine was 54.5% to 100.0%. The quality control assessment results from 2009 to 2020 were calculated according to │Z│≤2, the qualified rate of water arsenic was 84.6% - 100.0%, and that in urine was 83.6% - 100.0%. Among the 13 water arsenic assessment results from 2009 to 2020, according to │Z│≤2, provincial laboratories reported all qualified years for 8 times, prefecture, city and county-level laboratories reported all qualified years for 2 times; there were 8 provinces with all qualified laboratories at province level and 3 provinces with all qualified laboratories at prefecture, city and county-level. Among the 13 assessment results of urinary arsenic from 2009 to 2020, according to │Z│≤2, the provincial laboratories reported all qualified years for 6 times, and the prefecture, city and county-level laboratories all qualified for 1 times; there were 6 provinces in which all provincial laboratories were qualified, and there were 6 provinces in which all prefecture, city and county-level laboratories were qualified.Conclusions:The quality control work of laboratories is improving day by day, but there are still some problems in individual links. Laboratories at all levels should continue to strengthen the construction of laboratories and the training of relevant personnel, further improve the theoretical and technical level of inspectors, and continuously improve the detection quality, to make the analysis data more reliable and comparable, so as to ensure the quality of arsenic poisoning prevention and monitoring work.

Objective To investigate the efficacy of oxygen uptake efficiency slope (OUES) on evaluation the cardiopulmonary function of patients with chronic obstructive pulmonary disease (COPD). Methods The cardiopulmonary function of 54 stable COPD patients with the cardiopulmonary function of Ⅱ~Ⅳ were evaluated, following a symptom-limited Steep protocol with simultaneous respiratory gas measurement,they were performed exercise tests on a treadmill, simultaneously the oxygen uptake (VO2), carbon dioxide production (VCO2),peak oxygen uptake (VO2peak), minute ventilation (VE), and respiratory gas exchange rate (RER) were measured. OUES was derived from the relation between VO2 and VE during incremental exercise and was determined by VO2=algVE+b, where a=OUES, to measure anaerobic threshold (VAT) meanwhile. Results OUES correlated with the VO2peak (P<0.001). 75% OUES, 90% OUES and 100% OUES were not significantly different (F=0.239, P=0.830). Conclusion OUES can respond the cardiopulmonary function in patients with COPD, 75% OUES from sub-maximal exercise can be an index for cardiopulmonary function.

Aim To establish a model of the vascular endothelial cell (VEC) injury in SDrats.Methods SD rats were randomly divided into the control and the modelgroups. The model rats were injected with adrenaline diluted to 2. 5 times 0. 05 mg?100 g-1 (tid) for 5 d continously. From the 4th d, they were irritated for 5 min in the0℃ cold-water in the middle between adrenaline injections.The control rats weregiven 0. 9% NS as above. At 6th d, blood samples were taken from carotid arteries ofthe rats and the CEC counts, t - PA、PAI activities, 6-keto-PGF1? concentrations andthe platelet aggregation rate(max) were detected respectively. Results In the modelgroup, as compared with those in the control group, t - PA activity and 6-keto-PGF1?concentration decreased significantly(P

We retrospectively analyzed the clinical manifestations,treatments and prognosis of infective endocarditis (IE)associated with renal lesions in children.There were 23 confirmed IE cases who were admitted to the Capital Institutes of Pediatrics from 1992 to 2012.There were 6 cases (26％) with renal lesions.Renal lesions included asymptonatic microscopic hematuria(1 case),asymptomatic proteinuria (1 case) and acute nephritic syndrome (4 cases).There were 3 cases of acute renal injury (AKI) resulted from nephritis accompanied with acute heart failure.The serum level of C3 was lower in patients with renal lesions than patients without renal lesions (P ＜ 0.05),but the clinical manifestations,physical signs and other laboratory findings were the same.Patients of both groups were treated with antibiotics.All patients were recovered and discharged.The average hospital days were (39.2-± 15.2)days for patients with renal lesions and (34.9-± 19.2) days for patients without renal lesions (P ＞ 0.05).Renal lesions secondary to IE are relatively common in IE patients in children.Most of renal lesions can be improved by effective antibiotics.Prognosis of those patients accompanying with AKI is bad.

AIM: To study the effect of VEGF on extracellular H2O2 production in HUVECs and the role of H2O2 in the VEGF-induced proliferation. METHODS: HUVECs was stimulated with 500 ?g/L VEGF. Products of extracellular H2O2 was detected by H2DCFDA staining. MTT method was used to value the influences of 3?106 U/L catalase and 5-20 mmol/L H2O2 to VEGF function. RESULTS: After treatment for 15 min with VEGF, HUVECs appeared fluorescence, and continued to become stronger, peaked at 45 min then decreased. HUVECs, which was treated simultaneity with VEGF and 3?106 U/L catalase, only appeared very faint fluorescence. The proliferation of HUVECs by VEGF was restrained when treated with 3?106 U/L catalase. The extrinsic H2O2 at concentration of 5-10 mmol/L promoted the proliferation of HUVECs but inhibited the proliferation effect of VEGF on HUVECs (P

As the existing electrical field method for colon hollow structure virtual eversion is time consuming, a new faster method is presented here to overcome this disadvantage. In the new method, the cross section we used is composed of an orthogonal section and an electrical field section rather than the electrical field section only. We have applied this new method to a series of CT data of a patient for virtual eversion. The result shows that the time cost by using the fast method is only about 1/6 of the existing method, and the image quality is also acceptable.
Colon ; diagnostic imaging ; pathology ; Humans ; Image Interpretation, Computer-Assisted ; methods ; Image Processing, Computer-Assisted ; methods ; Phantoms, Imaging ; Tomography, X-Ray Computed ; User-Computer Interface

Objective To investigate the typical value of gamma-radiation dose rate and its confidence interval in tank-transported copper ore by using bootstrap resampling techniques. Methods Bootstrap resampling method, coupled with kernel density estimation, introduced to acquire the typical value of gamma-radiation dose rate in copper ore. Results The typical value of gamma-radiation dose rate in copper ore was expressed as the central tendency of the means of resampling, and two kinds of confidence interval, empirical percentile and bias-corrected accelerated confidence interval, were provided as standard error. Conclusion It is clearly demonstrated that this method has an advantage to give a robust description in explanation of central tendency and variation range of gamma-radiation dose rate data profiles.