Purpose　The aim is to investigate the effects of thymopeptide on LPS-induced release of nitric oxide (NO) from mouse peritoneal microphase in vitro.Methods　NO was detected by Griess reagent.Results　Thymopeptide 83.3～333.3 μg/ml reduced NO synthesis and secretion from mouse peritoneal microphase in vitro significantly.Conclusion　The effects of thymopeptide on LPS-induced release of NO from mouse peritoneal microphase may be one of mechanisms of its pharmacological effect.

OBJECTIVE:To investigate the effect of NGF on release of NO from peritoneal macrophages in mice METHODS:The content of NO in macrophages was detected with Griess agent RESULTS:NGF,in concentration of 0 73mg～5 83mg free amino acid per ml,could significantly inhibit release of NO from peritoneal macrophages in mice CONCLUSION:Inhibiting NO release may be one of the mechanism of NGF in protecting neurons

Objective:To observe the anesthetic effects of SumianxinⅡ combined with diazepam on the Beagle dogs in experimental periodontal surgery to Provide the reference. Methods:Healthy adult male Beagle dogs were anesthetized with SumianxinⅡat the doses of 0.05、0.1、0.15 ml/kg in the combination group and diazepam(0.1 ml) by hip intramuscular injection simultaniously. Added injection dosage was half of the first dosage. Healthy adult Beagle dogs in the contral group were only anesthetized with SumianxinⅡ at the doses of 0.05、0.1、0.15 ml/kg by hip intramuscular injection. The time of adding anesthetic was not sure in surgery. Anesthesia induction period,initial duration of anesthesia,and adverse reactions after anesthesia were compared between the two groups. Results: The combined treatment group induction dose respectively (11.00?1.41) min,(9.00?0.82) min,(12.75?0.50) min,For the first time maintaining anesthesia(29.75?6.62) min,(46.00?5.29) min,(45.25?6.02) min,Intraoperative additional one times longer cansustain anesthesia for(38.75?3.50) min,(45.25?4.03) min,(37.25?2.75) min. Pure Sumianxin Ⅱ new standards of sleep narcotic induction respectively respectively was (15.75?2.50) min,(14.25?2.99) min,(14.50?2.08) min,For the first time maintaining anesthesia was (20.75?5.19) min,(20.5?3.42) min,(22.25?5.19) min,the time of adding anesthetic was not sure in surgery. 2 Dogs died in SumianxinⅡ group. United drug group simple Sumianxin Ⅱ induction period in each group by t test,Sumianxin Ⅱat the doses of 0.1 ml/kg supplemented with 0.3 ml dose stability (P

The feasibility of screening for antibody to HIV by pooling 10 serum specimens was demonstrated in this experiment. The test was conducted using the reagent developed by Organon Teknika corporation (U. S. A) for testing for antibody to HIV by ELISA. The determination was made by changing only the ratio between the serum specimen volume and the serum specimen diluent volme, with their total volume being constant. When the serum specimen volume was increased from 5to 59?l as a result of pooling 10 serum specimens, while the serum specimen diluent volume was correspondingly decreased, the optical density of the pooled serum specimens was somewhat raised, but it was always below cutoff value, indicating that the specificity of test did not be subjected to any influence. In addition, when one serum specimen estabished as being positive intesting for antibody to HIV, and nine serum specimens from normal donors were pooled, the serum specimen containing the antibody to HIV had exactly been shown through multiple paired tests, indicating that the serumpooling method did not have any influence on the test sensitivity and could reach the level of original method. The serum-pooling method could be easy to spread because of its many advantages, such as saving the expenditure by 10 times, saving the manpower and material resource, and saving the working hours. (Original article on page 5)

Phototherapy is the most common and effective method to intervene and treat neonatal hyperbilirubinemia in clinic.The application of phototherapy varies from gestational age,birth weight,age and many other factors.Beside the traditional phototherapy instruments,more advanced high-intensive phototherapy instruments are applied to phototherapy.With the increasing application of phototherapy,many problems have showed up.This paper reviews based on the research of the indication,clinical application and adverse reactions on phototherapy.

Child ; Glomerulonephritis ; classification ; diagnosis ; therapy ; Humans

Child ; Early Diagnosis ; Evidence-Based Medicine ; Glomerulonephritis ; diagnosis ; therapy ; Glomerulonephritis, Membranous ; diagnosis ; therapy ; Humans ; Kidney Diseases ; diagnosis ; therapy ; Kidney Glomerulus ; pathology ; Nephrosis, Lipoid ; diagnosis ; therapy ; Renal Replacement Therapy

Objective To study the effect of using Dexamethasone in different time for treatment and prevention of bronchopulmonary dysplasia (BPD), in order to provide the clinical reference as to whether dexamethasone can be used to prevent and treat BPD.Methods A total of 135 very low birth weight infants hospitalized for 1 week who still survived on mechanical ventilation were studied.The total cases were divided into early Dexamethasone group and none Dexamethasone group.Whether early using dexamethasone could prevent BPD or not were observed.The BPD infants were divided into 3 groups, early Dexamethasone group, later Dexamethasone group and none Dexamethasone group.The effect of using Dexamethasone on BPD in different time were observed.Results (1) Among these 135 infants,there were 65 infants developing BPD.The incidence of BPD was 48.15％.There were no significant differences in the prevalence of BPD, the death rate and the disease index of BPD between the early Dexamethasone group and the none Dexamethasone group (all P ＞ 0.05).(2) There were no significant differences in mechanical ventilation time among the 3 BPD groups [(19.81 ± 5.67) d vs (22.32 ± 6.20) d, (21.46 ± 7.02) d, P =0.405].But the average time of inhaling oxygen and hospital stays of later Dexamethasone BPD group were shorter than early Dexamethasone BPD group and none Dexamethasone BPD group.The differences were significant [(37.27 ± 10.14) d vs (45.96 ± 9.91) d,(43.42 ±8.73) d,P=0.012;(64.11 ±8.14) d vs (76.13 ±7.57) d,(68.59 ±8.53) d,P =0.000].(3) The incidence of infection (90.90％, 100.00％ vs 81.90％), hyperglycemia (27.27 ％, 30.43 ％ vs 10.00 ％), hypertension(9.09％ ,13.04％ vs 0) and neonatal necrotizing enterocolitis(22.73％ ,34.78％ vs 15.00％) of all the infants who received Dexamethasone were higher than the infants who did not receive Dexamethasone.But the differences showed no significance(all P ＞ 0.05).(4) There were no significant differences among the 3 BPD groups in growth [weight : (9.16 ± 1.53) kg vs (8.92 ± 1.13) kg, (9.07 ± 1.46) kg;height: (71.26 ± 8.59) cm vs (69.54 ± 9.32) cm, (70.32 ± 9.07) cm] or been in hospital again because of pulmonary infection during one year(75.00％ vs 65.00％ ,72.22％) (all P ＞ 0.05).The differences of abnormal Chinese children development scale scores among the BPD groups (mental development index ＜ 80:20.00％ vs 15.00％, 16.67 ％;physical development index ＜ 80:25.00％ vs 20.00％, 16.67％) showed no significance(all P ＞ 0.05).Conclusions Early use of Dexamethasone has no effect to prevent BPD.Dexamethasone therapy on BPD should be used in the later stages ,and small doses and short protocol are preferred.

Computer technology is one of the greatest achievements of the 20th Century. Its appearance not only brings a breakthrough development in various fields, but also brings promotion to the development of traditional Chinese medicine (TCM). TCM is a traditional medicine, and the modernization of TCM is urgent. Whether in the medical management or scientific research, the application of computer technology has penetrated into all levels. The following article described the contribution made by computer technology, pointed out possible problems and provided relevant thinking based on the application of TCM related systems and data mining technology.

Antineoplastic Agents ; adverse effects ; Child ; Child, Preschool ; Hematopoietic Stem Cell Transplantation ; adverse effects ; Humans ; Kidney Diseases ; etiology ; pathology ; therapy ; Kidney Neoplasms ; etiology ; pathology ; therapy ; Neoplasms ; complications ; therapy ; Paraneoplastic Syndromes ; etiology ; pathology ; therapy ; Radiotherapy ; adverse effects ; Risk Factors ; Tumor Lysis Syndrome ; etiology ; pathology ; therapy