Objective To investigate the clinical features,diagnostic modalities and treatment of duodenal Brunner′gland adenoma(BGA).Methods Fifteen patients with duodenal BGA were evaluated base on clinical presentations,the results of serologic test,barium meal examination,gastroscopy and endoscopic ultrasonography(EUS)as well as treatment.The post-operative histological results were compared with pre-operation EUS findings.Results Eight out of 15 patients with duodenal BGA had presentations,including duodenal ulcer(4 patients),upper gastrointestinal hemorrhage (4 patients). The serologic tests of all patients were within the normal limits.Six patients underwent barium meal examination and 13 patients received gastroscopy.Seven patients underwent surgical treatment and 8 patients received endoscopic resection.The post-operative histological results were compatible with the pre-operative EUS findings.Conclusions Duodenal BGA is a benign tumor.EUS was helpful in making the diagnosis and determination of treatment methods(surgical operation or endoscopic resection).

Objective To evaluate the detection of alpha 5 (Ⅳ) collagen chain of skin basement membrane in diagnosis of Alport syndrome among suspected patients. Methods Data of suspected patients with the detection of alpha 5 (Ⅳ) collagen chain of skin basement membrane were retrospectively collected and analyzed from January 2007 to March 2008. Results A total of 254 suspected patients ranged from 1 to 71 years old with an average age of (25.85±17.03) years old were enrolled (male/female ratio, 0.76). There was no significant difference in average age between male and female. Abnormal alpha 5 (Ⅳ) collagen chain expression of skin basement membrane was found by indirect immunofluorescense in 19 patients among whom 12 cases were negative and 7 cases were diseontinous deposit. These 19 patients were diagnosed as Alport syndrome and the diagnostic rate was 7.5%. Conclusions The diagnostic rate of Alport syndrome by detection of alpha 5 (Ⅳ) collagen chain in skin basement membrane is significant and helpful for early and differential diagnosis of Alport syndrome.

The aim of this study was to investigate the clinical value of fluorescence in situ hybridization (FISH) in detecting the genomic aberration of chronic lymphocytic leukemia (CLL). FISH was used for 32 patients who were newly diagnosed as CLL. Five types of fluorescence probes with labeled DNA probes were included as sequence specific probes D13S25 for 13q14.3, P53 for 17p13.1, ATM for 11q22.3, RB1 for 13q14 and chromosomes 12. Meanwhile, FISH was used to detect IGH/CCND1 fusion gene in 10 CLL patients with untypical immunophenotypes. The results showed that out of 32 patients, 26 cases (81.3%) were abnormal including 14 cases of D13S25 deletion, 11 of RB1 deletion, 9 of trisomia 12, 6 cases of P53 deletion, and 1 of ATM deletion. 12 cases showed 1 kind of genomic aberration, including 7 cases of trisomia 12, 3 cases of D13S25 deletion, 1 of P53 deletion, 1 of ATM deletion. 11 eases displayed 2 kinds of abnormalities. Out of 11 cases, 7 were D13S25/RB1 deletion, 4 were of P53 deletion, and 3 cases had 3 kinds of abnormalities. Among 10 patients with CD5(+)CD23(-), two were positive with IGH/CCND1. It is concluded that the FISH can improve the detecting of chromosomal abnormalities in CLL, and every abnormality has its special feature. Detection of IGH/CCND1 seems important in diagnoses of CLL.
Aged ; Aged, 80 and over ; Chromosome Aberrations ; Female ; Humans ; In Situ Hybridization, Fluorescence ; methods ; Leukemia, Lymphocytic, Chronic, B-Cell ; diagnosis ; genetics ; Male ; Middle Aged ; Sequence Deletion

OBJECTIVETo investigate the effects of incorporating three different zinc oxide (ZnO) on the antibacterial activity of composite resin.

METHODSThe minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of nano-ZnO, tetrapod-like zinc oxide whiskers (T-ZnOw), micro-ZnO against Streptococcus mutans were examined by the broth dilution test. Then the three different ZnO were added to the powder of one kind of bicomponent self-cured composite resin at 5% respectively, and the antibacterial activities of the resin specimens were evaluated using the membrane covering method before and after 3-month accelerating aging.

RESULTSThe MIC values of the three different ZnO against Streptococcus mutans were 78.13, 312.50 and 1 250.00 microg/mL respectively and the MBC values were 156.25, 625.00, 1,250.00 microg/mL respectively. The antibacterial ratios of the resin specimens incorporating with 5% of the three different ZnO were (93.58+/-5.95)%, (89.42+/-4.11)% and (78.97+/-3.90)% respectively, while after 3-month accelerating aging those were (89.01+/-7.91)%, (84.63+/-4.72)% and (72.27+/-3.89)%.

CONCLUSIONThe three different ZnO could improve the antibacterial activity of the composite resin. The nano-ZnO exhibit the strongest antibacterial activity, while the micro-ZnO weakest. The T-ZnOw presents comparatively strong antibacterial activity although with smaller specific surface area.

Anti-Bacterial Agents ; Composite Resins ; Microbial Sensitivity Tests ; Streptococcus mutans ; Zinc Oxide

OBJECTIVETo investigate the relationship between pemphigus vulgaris (PV) and human leukocyte antigen (HLA) in Han nation of northeast China.

METHODSStandard microcytotoxicity test and polymerase chain reaction-sequence specific primers method were used to detect the HLA class I antigens and HLA-DRB1 and DQB1 alleles in 27 patients with PV and results were compared with control group.

RESULTSGene and phenotype frequencies of HLA-A3, A26(10), B60(40), and B13 (27.99%, 48%; 16.11%, 30%; 23.02%, 41%; 16.11%, 30%, respectively) increased significantly in PV group compared with control (1.01%, 2%; 0.5%, 1%; 4.61%, 9%; 5.13%, 10%, respectively). After P value correction, the difference of A3, A26 (10), and B60 (40) between the two groups was still significant. The gene frequencies of HLA-DRB1*140x (1401, 1404, 1405, 1407, 1408), DRB1*120x, and DQB1*0503 alleles in PV group (42.26%, 25.46%, and 23.02%) were significantly higher than control group (5.09%, 7.74%, and 1.89%). After P value correction, the difference was still significant between the two groups.

CONCLUSIONPV significantly relates with HLA in PV patients of Han nation of northeast China.

Adult ; Aged ; Asian Continental Ancestry Group ; ethnology ; China ; ethnology ; Female ; Gene Frequency ; HLA-A Antigens ; genetics ; HLA-A3 Antigen ; genetics ; HLA-B Antigens ; genetics ; HLA-DQ Antigens ; genetics ; HLA-DQ beta-Chains ; HLA-DR Antigens ; genetics ; HLA-DRB1 Chains ; Humans ; Male ; Middle Aged ; Pemphigus ; genetics ; Phenotype

Objective To observe the effects of applying 3D printing technology to interventional neuroradiology teaching so as to evaluate the feasibility and efficiency of the technology.Methods Computed tomography angiography was transformed into three-dimensional images and printed out as the case models. The 3D printed models were used in the teaching for interventional neuroradiology.Results The 3D printed entity model in diseases of the nervous systemnot only helps students deepen the understanding of anatomy and operation process, but also can significantly improve students' learning enthusiasm and help master teaching contents ( <0.05) . Conclusions The 3D print technique based on CT angiography has remarkable effect in the education of interventional neuroradiology.

Objective: To identify the relationship between Dietary Diversity and Nutritional Status in Elderly Inpatient. Methods: 136 elderly inpatients were selected in a tertiary hospital in Yunnan Provience. Food frequency questionnaire was used to collect dietary information and DDS9 was calculated; We used Mini Nutritional Assessment (MNA) to evaluate nutritional status, then analyzed the effect of Dietary Diversity on nutritional status in elderly inpatients. Results:① Patients at risk of malnutrition and with confirmed malnutrition in participants were 46. 3％, 33. 8％,, respectively. ② The incidence of insufficient diversity, moderate diversity and adequate diversity were 19. 9％, 77. 2％ and 2. 9％, respectively; the total scores of dietary diversity were (4. 68 ± 1. 27). However, apart from vegetables, the rest 8 kinds of food all showed lower scores of intra-group variety; while the consumption of beans, nuts and seafood was seriously inadequate. ③ The incidence of malnutrition risk and confirmed malnutrition in insufficient-diversity group and moderate-diversity group were 5. 109 times and 1. 094 times of adequacy group (P＜ 0. 05). Conclusion: Dietary diversity is strongly associated with putritional status in elderly inpatient. Improvement of dietary diversity status is potentially beneficial in the prevention of malnutrition.

BACKGROUND: With the development of 3D printing technology, organ and tissue construction can be achieved by constructing a three-dimensional scaffold that is conducive to cell growth. OBJECTIVE: To solve the scaffold over-accumulation during 3D printing.METHODS: Fluent, a finite element analysis software developed by ANSYS Company in the United States, was used to analyze the extrusion process of print heads and to obtain suitable viscosity and extrusion pressure of materials for the 3D printing of cellulose gel composites. We then compared simulation results with experimental results. RESULTS AND CONCLUSION: The error between simulation results and experimental results was less than 5%. The simulated values at a kinetic viscosity of 45 and a pressure of 0.10-0.12 MPa solved the phenomenon of over-accumulation of cellulose gel composites during the 3D printing process, ensuring enough space for the 3D printed scaffold.

Objective: To conduct an epidemiological investigation on a cluster of overseas imported cases of coronavirus disease 2019(COVID-19)in Qingtian County, Lishui,so as to provide reference for the prevention and control of COVID-19 imported from abroad. Methods: According to the COVID-19 Prevention and Control Program (FiFth Edition) issued by National Health Commission of China,a field investigation was employed and the close contacts of the case were tracked down ;the real-time reverse transcription polymerase chain reaction (RT-PCR) assay was performed to detect the nucleic acid of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from the sputum specimens or throat swabs of cases;the epidemiological characteristics,source of infection,route of transmission and disposal of the cluster were analyzed. Results: From March 1 to March 6,2020,eight confirmed cases and one asymptomatic case of COVID-19 were reported. Their median age was 33 years old. The nine cases all had no fever,no decrease in leukocyte and lymphocyte levels,and the clinical symptoms of seven cases were not obvious. The asymptomatic case had been tested negative for SARS-CoV-2 for six times,but had been weakly positive for IgM antibody and strongly positive for IgG antibody. Nine cases were all the employees of the same restaurant in Begamo,Italy. They lived in three independent villas and usually had lunch and dinner in the restaurant where they worked. Begamo had COVID-19 epidemic,but the staff of the restaurant did not take any protective measures such as wearing masks and environmental disinfection. Eight cases reported to have cold symptoms in Italy during mid February. Through a closed-loop management of“all people,all sites,all chains ”,such as treatment of current cases,screening and isolation of close contacts and health education,totally 372 close contacts were traced back,yet no fever or respiratory symptoms have been found. Conclusions It was a cluster of COVID-19 cases imported from abroad. The clinical symptoms of the cases were not obvious. Qingtian County adopted the closed-loop management in time,and effectively controlled the spread of the epidemic. No second-generation cases have been found.

OBJECTIVEPrader-Willi syndrome (PWS) is an example of a human genetic disorder that involves imprinting genes on the proximal long arm of chromosome 15 and SNRPN gene as a candidate gene for this syndrome. The purpose of this study was to show the molecular genetic defects and genomic imprinting basis in Chinese PWS patients and to evaluate the clinical applications of a differential diagnostic test for PWS.

METHODSFluorescence in situ hybridization (FISH) and methylation-specific PCR (MSPCR) techniques were applied for 4 clinically suspected PWS patients. Using three probes, including SNRPN probe for identification of the critical locus in PWS region, D15Z1 and PML control probes for identification of the 15p arm and 15q arm, the authors detected the deletions 15q in PWS. MSPCR was based on sodium bisulfite treatment of DNA and PCR primers specific for the maternal and paternal allele.

RESULTSWhen hybridized with mixed probes, it was found in 2 patients that the central specific signal was absent, but both the flanking control signals were retained, indicating SNRPN gene deletion of chromosome 15q11-13. Bisulfite-modified DNA from all PWS children amplified with methylated allele-specific primer pair showed only maternal 131bp PCR product, indicating the maternal uniparental disomy (UPD15).

CONCLUSIONGenomic imprinting plays an important role in the molecular pathogenesis of PWS that caused by paternal microdeletions of 15q11-q13 or maternal UPD of chromosome 15. The basic defect seemed to be an absence of function of PWS genes that are normally expressed only from the paternal chromosome 15. MSPCR is a rapid and simple PCR-based assay compared with other cyto-molecular tests and its results were consistent with the clinical diagnosis of PWS, so it seems to be a reliable diagnostic method for PWS patients who show abnormal methylation at SNRPN. The genetic differential tests for PWS are important in determining familial recurrence risk.

Adolescent ; Autoantigens ; Chromosome Deletion ; Chromosomes, Human, Pair 15 ; genetics ; Gene Deletion ; Genomic Imprinting ; genetics ; Humans ; In Situ Hybridization, Fluorescence ; Male ; Polymerase Chain Reaction ; methods ; Prader-Willi Syndrome ; genetics ; Ribonucleoproteins, Small Nuclear ; genetics ; snRNP Core Proteins