2.The antidiabetic and hepatoprotective effects of magnolol on diabetic rats induced by high-fat diet and streptozotocin.
Jun-Jun WANG ; Rong ZHAO ; Ji-Chao LIANG ; Yong CHEN
Acta Pharmaceutica Sinica 2014;49(4):476-481
The effects of magnolol (Mag) on hyperglycemia and hyperlipemia, hepatic oxidative stress and cytochrome P4502E1 (CYP2E1) activity of diabetic rats induced by high-fat diet (HFD) and streptozotocin (STZ) were studied. After oral administration of Mag (25, 50 and 100 mg x kg(-1) x d(-1)) for continuous 10 weeks, the blood glucose and lipids (TC, TG and LDL-C) levels, as well as the hepatic CYP2E1 activity and MDA content of diabetic rats, decreased significantly (P < 0.05 or P < 0.01), whereas the oral glucose tolerance and hepatic antioxidant enzymatic activities (CAT and GSH-Px) of diabetic rats, increased significantly (P < 0.05 or P < 0.01). The results indicated that Mag was effective against the hepatic oxidative damage, hyperglycemia and hyperlipemia of diabetic rats induced by HFD and STZ, and the inhibition of Mag on hepatic CYP2E1 activity could be an important mechanism of Mag against hepatic insulin resistance and oxidative damage.
Animals
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Biphenyl Compounds
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isolation & purification
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pharmacology
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Blood Glucose
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metabolism
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Cholesterol
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blood
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Cholesterol, LDL
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blood
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Cytochrome P-450 CYP2E1
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metabolism
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Diabetes Mellitus, Experimental
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blood
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drug therapy
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metabolism
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Diet, High-Fat
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Glucose Tolerance Test
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Hypoglycemic Agents
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isolation & purification
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pharmacology
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Lignans
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isolation & purification
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pharmacology
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Liver
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metabolism
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Magnolia
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chemistry
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Male
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Oxidative Stress
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drug effects
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Plants, Medicinal
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chemistry
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Protective Agents
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pharmacology
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Rats
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Rats, Wistar
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Streptozocin
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Triglycerides
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blood
5.Network pharmacology study of flavone extracts of Silybum marianum in the treatment of nonalcoholic fatty liver disease
Chen YE ; Wen JIANG ; Man HU ; Yan ZHANG ; Yu-cai LIU ; Ji-chao LIANG ; Yong CHEN
Acta Pharmaceutica Sinica 2022;57(2):399-408
To investigate the therapeutic effect and molecular mechanism of the main flavonoid components of
6.Experimental study of the monitoring bias of pressure in intubation balloon using handheld pressure gauge
Yanxia LIN ; Dan LIN ; Biqun CHEN ; Chao JI ; Congli YUAN ; Baochun WANG
Chinese Critical Care Medicine 2014;26(5):347-350
Objective To explore the bias between the real pressure and the measured values when handheld pressure gauge (HPG) was used to monitor intermittently the pressure in the intubation balloon,so as to provide some measures for the correct use of HPG.Methods In the first part of the study,HPG was used to measure the pressure with the balloon connected with a three-way tube with which to control the inflation and deflation in a laboratory to measure the pressure in the air bag.After gaining the deviation in this in vitro experiment,it was tested and verified in vivo in adult patients undergoing endotracheal intubation.Results After 132 times of measurements,it was found that measurement with a HPG might result in an inherent loss (3.928 ± 0.291) cmH2O (1 cmH2O=0.098 kPa,t =155.273,P =0.000) between inflation value [(30.000 ± 0.000) cmH2O] and measured value [(26.072 ± 0.291) cmH2O].In addition,after 214 times repeated measurements,the pressure loss during disconnection of the gauge was as high as (1.196 ± 0.954) cmH2O (t=18.348,P=0.000) between filled values [(30.000 ± 0.000) cmH2O] and measured values [(28.804 ± 0.954) cmH2O] and it was named as error loss.At last,the total error was verified by clinical test,and it was (5.270 ± 2.583) cmH2O (t=29.632,P=0.000) between pressure of filled value [(30.000 ± 0.000) cmH2O] and measured value [(24.730 ± 2.583) cmH2O].Conclusions When the balloon pressure was Monitored intermittently with HPG,the real value should be the measured value plus the error.In addition,subglottic aspiration should be done before the connection of the balloon to the gauge to prevent the secretions on the cuff falls into the deeper airway,and to maintain the cuff pressure at 30 cmH2O.
7.Five cases of subglottic cysts
Shuaishuai LIU ; Chao WANG ; Juan LI ; Lei WANG ; Jinshan JI ; Jinlu REN ; Shaochao WANG ; Chen MENG
Chinese Journal of Applied Clinical Pediatrics 2021;36(2):134-136
Objective:To summarize the clinical features and experience in diagnosis and treatment of subglottic cysts in children.Methods:The clinical data of 5 children with subglottic cysts admitted in Qilu Children′s Hospital of Shandong University from April 2018 to June 2019 were retrospectively analyzed.Results:Among the 5 patients, 2 cases were premature infants and 3 cases were full term infants.All patients had an endotracheal intubation history.One patient was asymptomatic.Four patients had laryngeal stridor, 2 cases of whom were accompanied by hoarseness and dyspnea.These 2 patients were used to be misdiagnosed with laryngomalacia, laryngitis, etc.Bronchoscopy revealed subglottic gray-white cyst-like lesions, and enhanced CT showed low density without enhancement.All patients were treated with laser and forceps, and bronchoscope was applied in the follow up period for 5-12 weeks.There was no recurrence or subglottic stenosis.Conclusions:Laryngeal stridor in children with a history of endotracheal intubation must be examined by bronchoscopy in time to find out whether there is subglottic cysts.Laser therapy is an effective treatment for subglottic cysts.
8.Screening of Poly-?-glutamic Acid High Productive Strain and Optimization of Fermentation Medium
Xue-Chao WU ; Xin-Jiang CAO ; Zhi-Xia JI ; Shou-Wen CHEN ;
Microbiology 2008;0(10):-
Bacillus subtilis B6-1 was used as an original strain for mutagenic treatment and a defined medium was used as the selective medium. A mutant named B. subtilis W003 was isolated after three serial ultraviolet (UV) irradiations and one diethyl sulfate (DES) treatment. The ?-PGA yield on a rotary shaker was enhanced from 10.9 g/L in parental strain to 20.5 g/L in the mutant. It was illustrated by single factor experiments that the optimal carbon and nitrogen sources were glucose and (NH4)2SO4 respectively. The optimal fermentation medium was achieved by orthogonal test. In the optimal medium, a ?-PGA yield of 45.3 g/L was obtained after 36 h cultivation.
9.Change of immunological response and cell proliferation in breast augmented by polyacrylamide hydrogel injection
Rui MA ; Zhen-Yu CHEN ; Hui-Chao LI ; Ji-Zhen REN ; Zhen-Hua ZHANG ;
Academic Journal of Second Military Medical University 1985;0(05):-
Objective:To study the change of immunological response and cell proliferation in breast tissues augmented by polyacrylamide hydrogel injection(PHI).Methods:The expression of CD68,CD25 and PCNA in 20 breast tissues with indurations,12 without indurations after breast augmentation by PHI,and 10 normal breast tissues was examined by immunohistochemistry P-V6000; analysis was also done by H-E staining.Results:Hyperplasia of fibrous tissue and infiltration of inflammatory cells and macrophages were found in the breast and adjacent tissues 3-8 years after PHI.Positive cells of CD68,CD25 and PCNA hardly existed in the normal tissues,but the breast tissues around the polyacrylamide hydrogel had many positive cells of CD68 and PCNA,especially in cases with indurations;there were significant differences between the 3 groups(P
10.A new research paradigm in modernization of traditional Chinese medicine: single cell pharmacology
Jia-yun CHEN ; Qiu-yan GUO ; Cheng-chao XU ; Ji-gang WANG
Acta Pharmaceutica Sinica 2021;56(12):3300-3312
Single cell "omics" technology enables the capture of genome, transcriptome, proteome and other omics information in a high-throughput and unbiased manner at single-cell resolution, allowing the characterization of the functional state of individual cells to reveal their heterogeneity and differential responses to drug treatment. This technology has wide application in pharmacological research, facilitating drug screening, efficacy evaluation, and mechanistic studies. We envision that, in the field of traditional Chinese medicine (TCM), single cell omics technology can be applied in the identification of active ingredients and drug targets, and elucidation of drug mechanism of action. In this article, we briefly introduce the single cell omics technology - particularly single cell transcriptome sequencing, and review its application in the field of modern drug research. Based on that, we propose the concept of "single cell pharmacology" and articulate how it can be applied to transform the pharmacological research of TCM and promote TCM modernization.